##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043477_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	561803
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53294482229536	31.0	31.0	34.0	30.0	34.0
2	30.556061466385906	31.0	31.0	34.0	26.0	34.0
3	31.431676228144028	31.0	31.0	34.0	28.0	34.0
4	35.04792071242055	37.0	35.0	37.0	32.0	37.0
5	35.35532918122545	37.0	35.0	37.0	33.0	37.0
6	35.82746087151546	37.0	35.0	37.0	35.0	37.0
7	33.7582266381632	37.0	35.0	37.0	32.0	37.0
8	34.79188790376698	37.0	35.0	37.0	31.0	37.0
9	36.81320676464882	39.0	37.0	39.0	32.0	39.0
10	36.700001601985036	39.0	35.0	39.0	32.0	39.0
11	36.22341639329089	38.0	35.0	39.0	32.0	39.0
12	35.97734615158694	38.0	35.0	39.0	31.0	39.0
13	36.06227271837281	38.0	35.0	39.0	32.0	39.0
14	37.43836184570036	40.0	35.0	41.0	32.0	41.0
15	37.64395348547445	40.0	35.0	41.0	33.0	41.0
16	37.620888460901774	40.0	35.0	41.0	33.0	41.0
17	37.612077543195745	39.0	36.0	41.0	33.0	41.0
18	37.28015870331771	39.0	36.0	40.0	33.0	41.0
19	36.90418171494278	37.0	35.0	40.0	33.0	41.0
20	36.534011032336956	37.0	35.0	40.0	32.0	41.0
21	36.4258289827573	36.0	35.0	40.0	32.0	41.0
22	36.352034431998405	36.0	35.0	40.0	32.0	41.0
23	36.24629451960919	36.0	35.0	40.0	32.0	41.0
24	36.05968996249575	35.0	35.0	40.0	32.0	41.0
25	35.926321148160476	35.0	35.0	40.0	31.0	41.0
26	35.65414745026281	35.0	35.0	40.0	31.0	41.0
27	35.590906420934026	35.0	35.0	40.0	31.0	41.0
28	35.51287906970949	35.0	35.0	40.0	31.0	41.0
29	35.44563307778705	35.0	35.0	40.0	31.0	41.0
30	35.19629478660669	35.0	35.0	40.0	30.0	41.0
31	34.67612490499339	35.0	34.0	40.0	27.0	41.0
32	34.45693597221802	35.0	34.0	40.0	25.0	41.0
33	34.319334357417105	35.0	34.0	40.0	24.0	41.0
34	34.096450179155326	35.0	34.0	40.0	23.0	41.0
35	33.81412701605367	35.0	33.0	40.0	22.0	41.0
36	33.63070684919803	35.0	33.0	40.0	21.0	41.0
37	33.53466784620232	35.0	33.0	40.0	21.0	41.0
38	33.44343301833561	35.0	33.0	40.0	20.0	41.0
39	33.27290705104814	35.0	33.0	40.0	19.0	41.0
40	33.2209279765327	35.0	33.0	40.0	18.0	41.0
41	33.13737199694555	35.0	33.0	39.0	18.0	41.0
42	33.01829822909454	35.0	33.0	39.0	18.0	41.0
43	32.859594911383525	35.0	33.0	39.0	18.0	41.0
44	32.64826104524184	35.0	33.0	39.0	17.0	41.0
45	32.451464303323405	35.0	33.0	39.0	15.0	41.0
46	32.43290975662287	35.0	32.0	39.0	17.0	41.0
47	32.381993332182276	35.0	32.0	38.0	17.0	41.0
48	32.31543619382595	35.0	32.0	38.0	15.0	41.0
49	32.15904863448576	35.0	32.0	38.0	15.0	40.0
50	32.00271803461356	35.0	32.0	37.0	15.0	40.0
51	31.558220586219726	35.0	31.0	37.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	7.0
14	9.0
15	32.0
16	93.0
17	157.0
18	382.0
19	667.0
20	1207.0
21	1930.0
22	2901.0
23	4671.0
24	7296.0
25	10777.0
26	13362.0
27	14475.0
28	14655.0
29	14884.0
30	16293.0
31	19196.0
32	23389.0
33	30915.0
34	55581.0
35	83865.0
36	66387.0
37	45111.0
38	56154.0
39	77357.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.99524388442212	18.643367870944086	23.88969087028727	33.471697374346526
2	33.32467074757522	19.804273028089916	28.71219982805361	18.15885639628126
3	21.001489846084837	20.887036915075214	41.08682224908019	17.024650989759756
4	21.13712457925643	24.28751715458978	37.421480483372285	17.15387778278151
5	18.867467777850955	29.265596659327205	36.17424613254112	15.692689430280721
6	74.33691169324479	2.2440250408061186	21.849651924251027	1.5694113416980686
7	70.95173931075483	7.53911958462308	19.700144000655033	1.808997103967049
8	70.7182054919607	3.7135793151691967	23.02604293675897	2.542172256111128
9	43.913435848509174	19.28718785766541	27.776640566177115	9.022735727648303
10	34.232284270464916	21.144778507768738	36.17727210427854	8.445665117487803
11	31.12016133769311	20.083908416295394	36.964736749358764	11.831193496652741
12	16.971429486848592	33.945884945434614	36.19542793470309	12.887257633013707
13	11.905774800063368	36.60642609598027	37.56654912843114	13.92124997552523
14	10.736503721055245	24.293747096402118	51.30606280137343	13.6636863811692
15	11.907732781775819	21.49614722598491	51.38936602332134	15.20675396891793
16	13.33777142521489	22.473892093847844	38.32375405613712	25.864582424800155
17	15.485143368760937	23.620557383994033	44.34045386016094	16.553845387084085
18	20.545280107083798	25.667360266855106	37.72372166043969	16.063637965621403
19	21.47710140387289	24.707415232741724	37.52721149584463	16.288271867540757
20	14.924626603987518	30.859215774924664	39.0806029871681	15.135554633919721
21	16.216360539192564	25.968355455560044	36.56993643679368	21.24534756845371
22	13.273336027041507	30.326466750800545	36.250963416001696	20.14923380615625
23	12.50865516916072	25.223966408153746	47.980163865269496	14.287214557416034
24	12.475369480049055	26.28216652456466	45.5999700962793	15.642493899106983
25	11.760706155004511	33.382876203936256	38.34742783502402	16.508989806035213
26	12.097657007883546	34.903338002823055	37.91524787158488	15.08375711770852
27	12.984978720298754	29.83002938752552	43.88566810785987	13.299323784315856
28	11.764088123416927	26.210967189566446	42.807710175986955	19.21723451102967
29	14.285434574041078	31.712717803215718	39.117448643029675	14.884398979713529
30	20.40394942711235	29.942168340147703	35.12957388977987	14.524308342960076
31	22.35552319941332	27.80939226027629	36.01440362547014	13.820680914840256
32	20.947556349823692	29.58047571835679	34.47365001610885	14.998317915710668
33	15.64373988746945	29.386457530486666	34.95994147414663	20.009861107897255
34	16.526077646434782	28.357983136437504	37.54376534123171	17.572173875896
35	24.12215669905643	26.836809344200724	33.95478486230938	15.086249094433457
36	17.0189550429599	32.8316153527126	34.835342637899764	15.314086966427734
37	16.88438829981328	32.58615564530628	33.52919083735758	17.00026521752287
38	16.31639560486505	33.42595180161017	34.29048972682595	15.967162866698825
39	20.3859715950253	28.002520456458935	33.32680672762516	18.284701220890597
40	13.957205639699325	27.98561061439686	36.06406516163139	21.993118584272423
41	17.013437094497537	28.60468883220631	33.10341881406828	21.27845525922788
42	17.34842996566412	27.7971103749891	34.65681030539175	20.197649353955033
43	17.030702933234604	30.19118801430395	35.05801855810667	17.720090494354782
44	14.135737972207341	36.86701566207372	31.120873331043086	17.876373034675854
45	17.402363461925265	32.61160940756813	33.33001069770008	16.656016432806517
46	16.75978946356641	30.74387285222756	32.991279861446095	19.505057822759937
47	15.951143016324226	28.745307518827772	37.704319841652676	17.59922962319532
48	17.581963784458253	26.868137051599938	37.24152416416431	18.308374999777502
49	17.38705560490065	31.716989763315613	33.73175294542749	17.16420168635625
50	14.8934768949258	31.66323426539196	33.96902472930903	19.47426411037321
51	15.631636000519755	27.871335681724734	37.20467850830273	19.29234980945278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	579.0
1	4176.5
2	7774.0
3	29969.5
4	52165.0
5	30278.0
6	8391.0
7	8353.5
8	8316.0
9	9963.0
10	11610.0
11	13074.0
12	14538.0
13	14008.5
14	13479.0
15	12178.0
16	10877.0
17	9348.5
18	7820.0
19	6924.0
20	6028.0
21	5667.5
22	5307.0
23	4846.5
24	4386.0
25	4227.0
26	4142.5
27	4217.0
28	4766.0
29	5315.0
30	5926.5
31	6538.0
32	6816.5
33	7095.0
34	9039.5
35	10984.0
36	12878.0
37	14772.0
38	15180.5
39	15589.0
40	20499.5
41	25410.0
42	30821.0
43	36232.0
44	40080.5
45	43929.0
46	42031.0
47	40133.0
48	39075.0
49	38017.0
50	38361.0
51	38705.0
52	36829.0
53	34953.0
54	30386.5
55	25820.0
56	21934.0
57	18048.0
58	15534.0
59	13020.0
60	10564.5
61	8109.0
62	7237.0
63	6365.0
64	5040.0
65	3715.0
66	3099.0
67	2483.0
68	2168.0
69	1853.0
70	1612.5
71	1372.0
72	1472.0
73	1572.0
74	1259.0
75	767.0
76	588.0
77	427.5
78	267.0
79	233.5
80	200.0
81	166.0
82	132.0
83	83.5
84	35.0
85	28.5
86	22.0
87	15.5
88	9.0
89	6.0
90	3.0
91	5.5
92	8.0
93	8.5
94	9.0
95	4.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	561803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.298711563637845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08442362809389	27.91578258824632
2	9.035974071345748	6.379164848818878
3	3.469102076680084	3.6736450076864204
4	1.8421321680725762	2.6009956825157077
5	1.2349401692749038	2.1795898416792463
6	0.7860622322692097	1.664819040476408
7	0.5678610560939954	1.40313345390993
8	0.4281353918143372	1.2090102164671492
9	0.32060384870101305	1.0185212503340324
>10	2.4493387972754093	18.682199134779207
>50	0.6024348744281829	14.880387347794667
>100	0.17490044741462912	8.837864441451842
>500	0.002557024085009198	0.549139744901681
>1k	0.001022809634003679	1.1901638192428612
>5k	0.0	0.0
>10k+	5.114048170018395E-4	7.815583581695688
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43295	7.706438021868876	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4743	0.8442461147412882	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1850	0.3292969243667264	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	815	0.14506864505885517	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	633	0.11267294763466909	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	572	0.10181504904744189	Illumina PCR Primer Index 8 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10840098753477642	0.0
2	0.0	0.0	0.0	0.36721057025327386	0.0
3	0.0	0.0	0.0	0.5852585336852953	0.0
4	0.0	0.0	0.0	0.7463470291187481	0.0
5	0.0	0.0	0.0	1.6560965320583905	0.0
6	0.0	0.0	0.0	1.9359099186013602	0.0
7	0.0	0.0	0.0	2.723908558694062	0.0
8	0.0	0.0	0.0	3.6260041331213966	0.0
9	0.0	0.0	0.0	4.278724036717497	0.0
10	0.0	0.0	0.0	4.732085802318607	0.0
11	0.0	0.0	0.0	5.217309270331415	0.0
12	0.0	0.0	0.0	5.610507597859036	0.0
13	0.0	0.0	0.0	5.787793942004582	0.0
14	0.0	0.0	0.0	5.922360685151201	0.0
15	0.0	0.0	0.0	6.413280099963866	0.0
16	0.0	0.0	0.0	6.964540951187516	0.0
17	0.0	0.0	0.0	7.706438021868876	0.0
18	5.339950124865834E-4	0.0	0.0	8.037870926285548	0.0
19	5.339950124865834E-4	0.0	0.0	8.44459712746283	0.0
20	5.339950124865834E-4	0.0	0.0	8.845449383502759	0.0
21	5.339950124865834E-4	0.0	0.0	9.231349779193062	0.0
22	8.89991687477639E-4	0.0	0.0	9.639499967070307	0.0
23	8.89991687477639E-4	0.0	0.0	9.965236924687124	0.0
24	0.004627956774883722	0.0	0.0	10.253060236417392	0.0
25	0.004627956774883722	0.0	0.0	10.528601662860469	0.0
26	0.004627956774883722	0.0	0.0	10.828350863202937	0.0
27	0.004627956774883722	0.0	0.0	11.106918261383438	0.0
28	0.004627956774883722	0.0	0.0	11.409515435125837	0.0
29	0.004627956774883722	0.0	0.0	11.78348994220394	0.0
30	0.004627956774883722	0.0	0.0	12.123110770145407	0.0
31	0.004627956774883722	0.0	0.0	12.42428395718784	0.0
32	0.004627956774883722	0.0	0.0	12.716379229018	0.0
33	0.004627956774883722	0.0	0.0	13.041048196609843	0.0
34	0.004627956774883722	0.0	0.0	13.427126590637643	0.0
35	0.004627956774883722	0.0	0.0	13.762475458479217	0.0
36	0.004627956774883722	0.0	0.0	14.091060389495961	0.0
37	0.004627956774883722	0.0	0.0	14.438157147612241	0.0
38	0.004627956774883722	0.0	0.0	14.781871937316106	0.0
39	0.004627956774883722	0.0	0.0	15.140538587369594	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTGG	45	3.8380676E-10	45.000004	1
GTCTAGG	65	0.0	45.000004	1
AATGCGG	130	0.0	45.000004	1
GCGATAG	45	3.8380676E-10	45.000004	8
ACGTAGG	45	3.8380676E-10	45.000004	1
ATCGGTC	45	3.8380676E-10	45.000004	13
ATCGCGG	45	3.8380676E-10	45.000004	1
AGTACGG	45	3.8380676E-10	45.000004	1
GCATAAC	40	6.8048394E-9	45.0	8
TCGCCGT	20	7.030205E-4	45.0	24
AACGTGT	20	7.030205E-4	45.0	19
AGTCCGT	25	3.8882514E-5	45.0	29
ACACGGC	20	7.030205E-4	45.0	2
TCACCTA	20	7.030205E-4	45.0	35
ACGCCAT	20	7.030205E-4	45.0	42
CACGGCA	20	7.030205E-4	45.0	3
CGTGCTA	20	7.030205E-4	45.0	38
CGACGGT	55	1.8189894E-12	45.0	27
GCGGTGA	20	7.030205E-4	45.0	23
ACGTTCT	20	7.030205E-4	45.0	44
>>END_MODULE