##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043476_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	900495
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48651130766967	31.0	31.0	33.0	30.0	34.0
2	30.531705339840865	31.0	31.0	34.0	26.0	34.0
3	31.397743463317397	31.0	31.0	34.0	28.0	34.0
4	35.02646766500647	37.0	35.0	37.0	32.0	37.0
5	35.33680697838411	37.0	35.0	37.0	33.0	37.0
6	35.79442417781331	37.0	35.0	37.0	35.0	37.0
7	33.766669442917504	37.0	35.0	37.0	32.0	37.0
8	34.77055508359291	37.0	35.0	37.0	31.0	37.0
9	36.717884052659926	39.0	37.0	39.0	32.0	39.0
10	36.67914424844113	39.0	35.0	39.0	32.0	39.0
11	36.37340351695456	38.0	35.0	39.0	32.0	39.0
12	36.208660792119886	39.0	35.0	39.0	32.0	39.0
13	36.27629137307814	39.0	35.0	39.0	32.0	39.0
14	37.64694973320229	40.0	35.0	41.0	33.0	41.0
15	37.83544161822109	40.0	35.0	41.0	33.0	41.0
16	37.782060977573444	40.0	35.0	41.0	33.0	41.0
17	37.752722669198604	40.0	36.0	41.0	33.0	41.0
18	37.42200012215504	39.0	36.0	41.0	33.0	41.0
19	37.091964974819405	38.0	35.0	40.0	33.0	41.0
20	36.74033836945236	38.0	35.0	40.0	32.0	41.0
21	36.64089084336948	37.0	35.0	40.0	32.0	41.0
22	36.52392961648871	37.0	35.0	40.0	32.0	41.0
23	36.41552368419592	37.0	35.0	40.0	32.0	41.0
24	36.20771575633402	36.0	35.0	40.0	32.0	41.0
25	36.05083648437804	36.0	35.0	40.0	31.0	41.0
26	35.77963675534012	35.0	35.0	40.0	31.0	41.0
27	35.70202832886357	35.0	35.0	40.0	31.0	41.0
28	35.60092282577916	35.0	35.0	40.0	31.0	41.0
29	35.52868699992782	35.0	35.0	40.0	31.0	41.0
30	35.339283394133226	35.0	35.0	40.0	30.0	41.0
31	34.85568492884469	35.0	34.0	40.0	27.0	41.0
32	34.70183621230545	35.0	34.0	40.0	25.0	41.0
33	34.580635095142114	35.0	34.0	40.0	25.0	41.0
34	34.40643312844602	35.0	34.0	40.0	24.0	41.0
35	34.2006985047113	35.0	34.0	40.0	23.0	41.0
36	34.018507598598546	35.0	33.0	40.0	22.0	41.0
37	33.958050849810384	35.0	33.0	40.0	22.0	41.0
38	33.86837794768433	35.0	33.0	40.0	21.0	41.0
39	33.72590852808733	35.0	33.0	40.0	21.0	41.0
40	33.671669470680015	35.0	33.0	40.0	21.0	41.0
41	33.60173682252539	35.0	33.0	40.0	21.0	41.0
42	33.49685672879916	35.0	33.0	40.0	20.0	41.0
43	33.31999511379852	35.0	33.0	40.0	19.0	41.0
44	33.11438264510075	35.0	33.0	40.0	18.0	41.0
45	32.932259479508495	35.0	33.0	39.0	18.0	41.0
46	32.93191744540503	35.0	33.0	39.0	18.0	41.0
47	32.92372861592791	35.0	33.0	39.0	18.0	41.0
48	32.86888322533718	35.0	33.0	39.0	18.0	41.0
49	32.76974886034903	35.0	33.0	39.0	18.0	41.0
50	32.64330507109978	35.0	33.0	39.0	18.0	41.0
51	32.260326820248864	35.0	32.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	3.0
12	3.0
13	15.0
14	28.0
15	69.0
16	138.0
17	313.0
18	604.0
19	1161.0
20	1928.0
21	3006.0
22	4474.0
23	6972.0
24	10956.0
25	15985.0
26	19505.0
27	21389.0
28	21735.0
29	22471.0
30	24916.0
31	29119.0
32	35887.0
33	46953.0
34	81948.0
35	122419.0
36	116157.0
37	76661.0
38	92722.0
39	142883.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.908405932292794	18.45718188329752	24.29763630003498	33.3367758843747
2	33.744773707794046	19.707494211516998	28.750742647099653	17.796989433589303
3	21.235986873886027	20.683401906729078	40.969799943364485	17.11081127602041
4	21.26419358241856	23.883530724768047	37.67761064747722	17.174665045336177
5	18.47494988867234	30.35330568187497	35.30902448097991	15.862719948472783
6	75.33600963914292	2.064864324621458	21.195009411490346	1.404116624745279
7	71.7430968522868	7.282439102937828	19.195664606688542	1.77879943808683
8	71.05491979411323	3.702741270079234	22.473528448242355	2.7688104875651725
9	40.07018362123054	21.204226564278535	28.10420935152333	10.62138046296759
10	29.09721875190867	24.74394638504378	35.565105858444525	10.593729004603023
11	27.102315948450574	22.286742291739543	37.960121932936886	12.650819826872997
12	16.48915318796884	32.747322306064994	36.403311512001736	14.36021299396443
13	12.052815395976657	35.4176314138335	37.57622196680715	14.953331223382696
14	10.656250173515676	27.01669637255065	47.46433905796256	14.862714395971105
15	12.697905041116275	24.010683013231613	46.797816756339564	16.493595189312547
16	13.92023276087041	25.914191639042972	36.66527854124676	23.500297058839863
17	15.701031099561908	26.10619714712464	41.80867189712325	16.384099856190208
18	18.619648082443547	27.76284154825957	37.33457709370957	16.282933275587315
19	19.66207474777761	27.934635950227378	36.52124664767712	15.882042654317901
20	15.217963453433944	31.300673518453735	37.500930044031335	15.980432984080977
21	16.17943464427898	27.73552323999578	36.23840221211667	19.84663990360857
22	12.633051821498176	31.335099028867457	36.12268807711314	19.909161072521226
23	12.25226125630903	27.967506760170796	45.614578648410046	14.165653335110134
24	13.380196447509427	28.555738788110986	42.606677438519924	15.457387325859667
25	12.600181011554756	33.21040094614629	37.185547948628255	17.003870093670702
26	12.749765406804038	34.14288807822364	36.823969039250635	16.283377475721686
27	12.954763768816042	31.503006679659524	41.07696322578138	14.465266325743064
28	12.352872586743956	27.519308824590922	40.75125347725418	19.376565111410944
29	13.951770970410719	32.197180439647084	38.68905435343894	15.161994236503256
30	18.267730525988483	31.29045691536322	34.80674517904042	15.63506737960788
31	18.36445510524767	29.91921110056136	36.28326642568809	15.43306736850288
32	17.186880549031365	31.21427659231867	35.26160611663585	16.337236742014113
33	13.76476271384072	31.500119378786113	35.76388541857534	18.971232488797828
34	13.678365787705651	31.19617543684307	37.0461801564695	18.079278618981782
35	19.616988434139003	30.242366698315926	33.883808349852025	16.256836517693046
36	13.752214060044754	35.05694090472463	35.74689476343566	15.443950271794957
37	14.655828183388026	33.80562912620281	34.92012726333849	16.61841542707067
38	13.982753929783065	34.74000410885124	34.336892486909974	16.940349474455715
39	17.46272883247547	31.12665811581408	33.73522340490508	17.675389646805368
40	13.027723640886402	30.4071649481674	35.81652313449825	20.748588276447954
41	15.564550608276559	30.90544644889755	32.65004247663785	20.87996046618804
42	15.244726511529768	29.372622835218408	35.42240656527799	19.960244087973837
43	14.971765528959072	31.62727166724968	35.484816684157046	17.916146119634202
44	13.021282738938028	36.78421312722447	32.718116147230134	17.476387986607367
45	16.260056968667232	33.16409308213816	33.971315776323024	16.60453417287159
46	15.122238324477093	33.17630858583335	32.94843391690126	18.7530191727883
47	14.643945829793614	31.162083076530127	36.17843519397665	18.015535899699607
48	15.581652313449826	29.10099445305082	36.65850448919761	18.658848744301746
49	15.782541824218901	32.5271100894508	33.998523034553216	17.691825051777077
50	13.955768771620052	31.612724112849044	34.69780509608604	19.73370201944486
51	13.728782502956708	29.959633312789073	36.4777150345088	19.833869149745418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1519.0
1	7159.0
2	12799.0
3	50283.0
4	87767.0
5	50168.5
6	12570.0
7	12933.0
8	13296.0
9	16609.5
10	19923.0
11	22696.0
12	25469.0
13	24680.5
14	23892.0
15	21275.0
16	18658.0
17	15638.0
18	12618.0
19	10806.5
20	8995.0
21	8227.0
22	7459.0
23	6783.5
24	6108.0
25	6218.5
26	7521.5
27	8714.0
28	8787.0
29	8860.0
30	10477.5
31	12095.0
32	13361.5
33	14628.0
34	17883.0
35	21138.0
36	22381.0
37	23624.0
38	26399.0
39	29174.0
40	36726.0
41	44278.0
42	52358.5
43	60439.0
44	66063.5
45	71688.0
46	73008.0
47	74328.0
48	69363.0
49	64398.0
50	56897.5
51	49397.0
52	44010.5
53	38624.0
54	34875.5
55	31127.0
56	27703.5
57	24280.0
58	22129.0
59	19978.0
60	16803.0
61	13628.0
62	11621.5
63	9615.0
64	7837.0
65	6059.0
66	5295.5
67	4532.0
68	4068.0
69	3604.0
70	3112.0
71	2620.0
72	2569.0
73	2518.0
74	2077.5
75	1326.5
76	1016.0
77	780.0
78	544.0
79	416.5
80	289.0
81	201.5
82	114.0
83	101.5
84	89.0
85	52.5
86	16.0
87	13.5
88	11.0
89	10.0
90	9.0
91	7.5
92	6.0
93	7.5
94	9.0
95	8.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	900495.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.11832042301896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4645947826845	25.986155918752868
2	9.214881128790438	6.103627717666247
3	3.4136245364213176	3.3916053360324225
4	1.8693565900302405	2.4763980213401435
5	1.1110442190311975	1.839795922500903
6	0.8234254729229592	1.636228119404309
7	0.6179018470334755	1.4324709952020922
8	0.46135727970139184	1.222350257491446
9	0.35532937647632595	1.059112093126905
>10	2.8522994547544958	20.651567026274545
>50	0.6397693526682237	14.772426151526261
>100	0.17083758922504086	8.431010755034011
>500	0.003137833271480333	0.7389850148899236
>1k	0.0017432407063779627	0.8968275954140683
>5k	3.486481412755926E-4	0.8744271121502174
>10k+	3.486481412755926E-4	8.48701196319362
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73502	8.162399569125869	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7573	0.840981904397026	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3079	0.3419230534317237	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1296	0.14392084353605517	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1281	0.14225509303216563	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1071	0.11893458597771227	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	1040	0.11549203493634057	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	996	0.11060583345826461	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1215997867839355	0.0
2	0.0	0.0	0.0	0.3827894657938134	0.0
3	0.0	0.0	0.0	0.6214359879843864	0.0
4	0.0	0.0	0.0	0.7823474866601147	0.0
5	0.0	0.0	0.0	1.7236075713912904	0.0
6	0.0	0.0	0.0	2.0201111610836264	0.0
7	0.0	0.0	0.0	2.803569148079667	0.0
8	0.0	0.0	0.0	3.700964469541752	0.0
9	0.0	0.0	0.0	4.319624206686322	0.0
10	0.0	0.0	0.0	4.78725589814491	0.0
11	0.0	0.0	0.0	5.306747955291256	0.0
12	0.0	0.0	0.0	5.709193277030966	0.0
13	0.0	0.0	0.0	5.885429680342479	0.0
14	0.0	0.0	0.0	6.019022870754418	0.0
15	0.0	0.0	0.0	6.480324710298225	0.0
16	0.0	0.0	0.0	7.021360473961543	0.0
17	0.0	0.0	0.0	7.663118618093382	0.0
18	0.0	0.0	0.0	7.99215986762836	0.0
19	0.0	0.0	0.0	8.35373877700598	0.0
20	0.0	0.0	0.0	8.759515599753469	0.0
21	0.0	0.0	0.0	9.151078018201101	0.0
22	1.1105003359263516E-4	0.0	0.0	9.558409541418886	0.0
23	1.1105003359263516E-4	0.0	0.0	9.877123137829749	0.0
24	6.66300201555811E-4	0.0	0.0	10.152194071038707	0.0
25	6.66300201555811E-4	0.0	0.0	10.423267203038328	0.0
26	6.66300201555811E-4	0.0	0.0	10.722435993536887	0.0
27	6.66300201555811E-4	0.0	0.0	10.999505827350513	0.0
28	7.773502351484462E-4	0.0	0.0	11.294454716572552	0.0
29	7.773502351484462E-4	0.0	0.0	11.655256275715024	0.0
30	7.773502351484462E-4	0.0	0.0	11.992071027601487	0.0
31	7.773502351484462E-4	0.0	0.0	12.28935196752897	0.0
32	7.773502351484462E-4	0.0	0.0	12.590408608598604	0.0
33	7.773502351484462E-4	0.0	0.0	12.89746195148224	0.0
34	7.773502351484462E-4	0.0	0.0	13.289024369929871	0.0
35	7.773502351484462E-4	0.0	0.0	13.602962814896252	0.0
36	7.773502351484462E-4	0.0	0.0	13.911459808216591	0.0
37	7.773502351484462E-4	0.0	0.0	14.244165708860127	0.0
38	7.773502351484462E-4	0.0	0.0	14.573318008428698	0.0
39	7.773502351484462E-4	0.0	0.0	14.92445821464861	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGG	20	7.0324715E-4	45.0	1
GATACGC	55	1.8189894E-12	45.0	8
GTACCGG	25	3.8901308E-5	45.0	1
CCCTCTA	20	7.0324715E-4	45.0	32
GCGACAT	25	3.8901308E-5	45.0	36
CTCGTCA	25	3.8901308E-5	45.0	19
CATGCGG	210	0.0	45.0	1
ATTACGA	25	3.8901308E-5	45.0	1
CAACCCG	25	3.8901308E-5	45.0	22
GTAATCG	20	7.0324715E-4	45.0	8
CTAACGG	80	0.0	45.0	1
GATGCGT	20	7.0324715E-4	45.0	10
AGTTCGG	25	3.8901308E-5	45.0	1
TAGATCA	25	3.8901308E-5	45.0	27
CATCGCG	35	1.2115379E-7	45.0	18
ATGCGAC	20	7.0324715E-4	45.0	11
TGAGGCG	20	7.0324715E-4	45.0	18
CACACGG	50	2.1827873E-11	45.0	1
GGCGAAT	20	7.0324715E-4	45.0	7
GCGTCAG	35	1.2115379E-7	45.0	8
>>END_MODULE