##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043475_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	418557
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.543720449066676	31.0	31.0	34.0	30.0	34.0
2	30.59822915397425	31.0	31.0	34.0	26.0	34.0
3	31.464202008328616	31.0	31.0	34.0	28.0	34.0
4	35.059812642005745	37.0	35.0	37.0	32.0	37.0
5	35.35359102822316	37.0	35.0	37.0	33.0	37.0
6	35.80695341375249	37.0	35.0	37.0	35.0	37.0
7	33.863992240005544	37.0	35.0	37.0	32.0	37.0
8	34.825963966675985	37.0	35.0	37.0	32.0	37.0
9	36.82895280690563	39.0	37.0	39.0	32.0	39.0
10	36.721192095700225	39.0	35.0	39.0	32.0	39.0
11	36.09473261706291	37.0	35.0	39.0	32.0	39.0
12	35.678942175139824	38.0	35.0	39.0	31.0	39.0
13	35.80228021512004	38.0	35.0	39.0	31.0	39.0
14	37.19972429083733	39.0	35.0	41.0	32.0	41.0
15	37.426106838495116	39.0	35.0	41.0	33.0	41.0
16	37.37240566995654	39.0	35.0	41.0	33.0	41.0
17	37.37666315460021	39.0	35.0	41.0	33.0	41.0
18	37.103536674813704	39.0	35.0	40.0	32.0	41.0
19	36.78991630769525	37.0	35.0	40.0	32.0	41.0
20	36.38939021447497	36.0	35.0	40.0	32.0	41.0
21	36.34365689738793	36.0	35.0	40.0	32.0	41.0
22	36.30004993346187	36.0	35.0	40.0	32.0	41.0
23	36.20872187061738	35.0	35.0	40.0	32.0	41.0
24	36.056085551071895	35.0	35.0	40.0	32.0	41.0
25	35.95097441925473	35.0	35.0	40.0	31.0	41.0
26	35.670087945011076	35.0	35.0	40.0	31.0	41.0
27	35.64118387698689	35.0	35.0	40.0	31.0	41.0
28	35.58500992696335	35.0	35.0	40.0	31.0	41.0
29	35.504870304402985	36.0	35.0	40.0	31.0	41.0
30	35.30177729676006	35.0	35.0	40.0	30.0	41.0
31	34.82635817821706	35.0	34.0	40.0	28.0	41.0
32	34.598592306424216	35.0	34.0	40.0	26.0	41.0
33	34.46501671218018	35.0	34.0	40.0	25.0	41.0
34	34.278822239264905	35.0	34.0	40.0	24.0	41.0
35	34.02684222220629	35.0	34.0	40.0	23.0	41.0
36	33.81764490857876	35.0	33.0	40.0	22.0	41.0
37	33.772996270519904	35.0	33.0	40.0	22.0	41.0
38	33.717328822597636	35.0	33.0	40.0	21.0	41.0
39	33.527736962946506	35.0	33.0	40.0	21.0	41.0
40	33.51806563980533	35.0	33.0	40.0	21.0	41.0
41	33.42420984477622	35.0	33.0	40.0	20.0	41.0
42	33.297789787293006	35.0	33.0	40.0	20.0	41.0
43	33.111289501788285	35.0	33.0	39.0	18.0	41.0
44	32.88864838002948	35.0	33.0	39.0	18.0	41.0
45	32.74584823572417	35.0	33.0	39.0	18.0	41.0
46	32.761941623243665	35.0	33.0	39.0	18.0	41.0
47	32.7332860279484	35.0	33.0	39.0	18.0	41.0
48	32.686656775540726	35.0	33.0	39.0	18.0	41.0
49	32.515215370905274	35.0	33.0	39.0	18.0	41.0
50	32.36754850593826	35.0	32.0	38.0	17.0	40.0
51	31.919709860305765	35.0	31.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	5.0
13	3.0
14	8.0
15	19.0
16	53.0
17	127.0
18	296.0
19	516.0
20	905.0
21	1411.0
22	2183.0
23	3374.0
24	5374.0
25	7866.0
26	9863.0
27	10376.0
28	10323.0
29	10438.0
30	11739.0
31	13976.0
32	17160.0
33	23222.0
34	44359.0
35	65195.0
36	41162.0
37	32578.0
38	42521.0
39	63446.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.934716179636226	18.67630931987758	24.260256070260443	32.12871843022575
2	31.886218603439914	21.32349954725402	28.759523792458374	18.03075805684769
3	21.83669129891508	22.371624414356944	38.40743315725218	17.384251129475796
4	22.793789137441255	25.11485890810571	34.497332501905355	17.594019452547684
5	18.492343934040047	31.603580874289523	32.99574490451719	16.90833028715324
6	77.54117121443436	2.3621633373710154	18.621358620211822	1.4753068279828077
7	74.30576958454881	7.1901795932214725	16.594872382972927	1.9091784392567799
8	73.5486445095889	3.8637509347591843	19.897170516799385	2.690434038852534
9	44.972847186882554	21.32373846333952	24.424630337086704	9.278784012691222
10	37.039638567745854	20.12509646236952	33.11950343680789	9.715761533076737
11	34.32435725600097	19.44346887042864	33.99608655451946	12.23608731905093
12	16.16721258992204	38.24425347085344	33.08557735266643	12.502956586558103
13	10.932561156544987	40.729697508344145	35.21479750667173	13.122943828439137
14	10.142704577871115	22.961508229464563	54.36822224929938	12.527564943364942
15	11.529612454217705	19.03229428727748	52.45354874007603	16.984544518428795
16	12.305372983846883	20.14683782615032	35.59873565607551	31.94905353392728
17	15.891742343336752	22.306878155185554	44.593926275274335	17.207453226203363
18	22.417257386688075	24.303260965651035	35.67566663560758	17.603815012053317
19	23.790308130075474	23.34664095929587	35.36579247270981	17.497258437918852
20	14.180625338962196	28.922464562771616	38.783964908005366	18.112945190260824
21	17.322610779415946	24.242098447762192	33.89669746295009	24.53859330987177
22	12.130964241429483	29.44234596482677	34.865502189665925	23.56118760407782
23	12.066217982258092	23.62665061150572	49.708402917643234	14.598728488592952
24	12.212673542671608	24.940211249602804	45.63942306543672	17.207692142288863
25	12.396161096338133	32.94342228179197	36.51927933351969	18.14113728835021
26	12.59637277598989	34.73338159438261	35.93512950446415	16.73511612516336
27	12.336909907133316	29.064858549731575	44.469450994727126	14.128780548407981
28	11.594597629474599	24.869253172208325	42.267361434643306	21.268787763673767
29	14.269979954940426	31.98632444326579	37.961376825617535	15.782318776176243
30	24.673819814266633	28.770275016306023	31.731639896119283	14.824265273308058
31	25.0708983483731	26.077451816598458	33.902192532916665	14.94945730211178
32	23.372204980444717	29.47651096505374	30.8173080368982	16.333976017603337
33	16.583882243039778	28.506033825739387	33.10039014996763	21.809693781253213
34	15.391929892463871	26.927993080990163	37.110118812969326	20.569958213576644
35	26.697200142393985	26.932293570529225	30.99100003105909	15.3795062560177
36	16.522241892979928	33.083666023982396	35.02724837955165	15.366843703486024
37	17.223699520017586	32.81464651170569	32.320090214713886	17.641563753562835
38	16.353567136614608	32.507639341833965	33.633411936725466	17.50538158482596
39	21.58152891959757	25.38889565817798	32.80915144173912	20.220423980485332
40	14.193048975408368	24.62555876499497	35.489789921086015	25.691602338510645
41	18.766619600197824	25.124893383696843	31.385689404310526	24.722797611794807
42	17.86925078304747	25.147351495734156	33.60402525820856	23.37937246300982
43	16.94106179086719	29.205102291922007	34.57187432058238	19.281961596628417
44	13.809349742090085	39.25797442164389	29.10356295558311	17.829112880682917
45	18.439782395229322	32.965402561658266	31.922772764521916	16.67204227859049
46	17.615521900242978	30.114177997262022	31.65136409139018	20.618936011104818
47	17.574667249621914	26.869936472212864	36.90441206335099	18.65098421481423
48	18.503095157887696	23.745391906000858	37.40756933942092	20.343943596690533
49	18.15332200871088	31.77990094539095	32.293809445308526	17.772967600589645
50	14.993895694015391	30.635492895830197	33.10803546470373	21.262575945450678
51	16.61231325721467	25.456269994289904	37.52702738217256	20.404389366322867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	664.0
1	2531.5
2	4399.0
3	16548.0
4	28697.0
5	16774.5
6	4852.0
7	4845.5
8	4839.0
9	5788.5
10	6738.0
11	7368.5
12	7999.0
13	7936.5
14	7874.0
15	6973.5
16	6073.0
17	5318.0
18	4563.0
19	4103.5
20	3644.0
21	3388.5
22	3133.0
23	2790.0
24	2447.0
25	2507.0
26	2962.0
27	3357.0
28	3430.0
29	3503.0
30	3983.0
31	4463.0
32	5567.0
33	6671.0
34	7386.5
35	8102.0
36	9606.5
37	11111.0
38	12813.5
39	14516.0
40	19317.5
41	24119.0
42	30170.0
43	36221.0
44	40543.5
45	44866.0
46	42967.5
47	41069.0
48	37133.5
49	33198.0
50	27911.0
51	22624.0
52	20267.5
53	17911.0
54	15715.0
55	13519.0
56	12668.0
57	11817.0
58	10860.0
59	9903.0
60	8343.0
61	6783.0
62	5398.0
63	4013.0
64	3706.5
65	3400.0
66	2957.5
67	2515.0
68	2032.5
69	1550.0
70	1524.5
71	1499.0
72	1483.0
73	1467.0
74	1177.5
75	695.5
76	503.0
77	387.0
78	271.0
79	199.5
80	128.0
81	82.5
82	37.0
83	30.0
84	23.0
85	19.0
86	15.0
87	9.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	418557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.25892962946845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49427962130792	28.381421407424522
2	8.583449686336532	6.052864969372456
3	3.239216779078671	3.4263394940438485
4	1.691015141160733	2.384935354582078
5	1.0120158035501405	1.7841297000642182
6	0.7124497359940845	1.507212906356928
7	0.4634142997980288	1.1437644530107665
8	0.3707627199564512	1.0458157321739865
9	0.28381698410085776	0.9006374763054098
>10	2.245601314598773	18.455709411522466
>50	0.6920012474184546	17.163117668744803
>100	0.20787389250514843	10.619930704663368
>500	0.002735182796120374	0.698946129513692
>1k	6.837956990300935E-4	0.6273397133475773
>5k	0.0	0.0
>10k+	6.837956990300935E-4	5.807834878873861
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24089	5.7552495836887205	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2602	0.6216596544795571	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1000	0.2389160855032887	No Hit
CAAGGGGCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAG	805	0.19232744883014738	No Hit
GCATCGGGATTTAACAAAACTATTTGCCAGAGAACTACTAGCCATAGCTTA	569	0.13594325265137128	No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	525	0.12543094488922654	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	487	0.11635213364010158	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	472	0.11276839235755225	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	467	0.11157381193003582	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	437	0.10440632936493716	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12399744837620683	0.0
2	0.0	0.0	0.0	0.4061573453555908	0.0
3	0.0	0.0	0.0	0.6715931163497445	0.0
4	0.0	0.0	0.0	0.8362062992615104	0.0
5	0.0	0.0	0.0	1.8714296977472602	0.0
6	0.0	0.0	0.0	2.135193056142891	0.0
7	0.0	0.0	0.0	2.9682934462928587	0.0
8	0.0	0.0	0.0	3.902455340610717	0.0
9	0.0	0.0	0.0	4.60224055504985	0.0
10	0.0	0.0	0.0	5.051402795796032	0.0
11	0.0	0.0	0.0	5.606643778505675	0.0
12	0.0	0.0	0.0	6.0971385020439275	0.0
13	0.0	0.0	0.0	6.291377279558101	0.0
14	0.0	0.0	0.0	6.462202280692952	0.0
15	0.0	0.0	0.0	7.071677214811841	0.0
16	0.0	0.0	0.0	7.729413198202395	0.0
17	0.0	0.0	0.0	8.726648939093122	0.0
18	0.0	0.0	0.0	9.095296459024697	0.0
19	0.0	0.0	0.0	9.549475937566449	0.0
20	0.0	0.0	0.0	9.960411604632105	0.0
21	0.0	0.0	0.0	10.318068984630528	0.0
22	2.3891608550328868E-4	0.0	0.0	10.724226329986118	0.0
23	2.3891608550328868E-4	0.0	0.0	11.042701471962003	0.0
24	0.002389160855032887	0.0	0.0	11.28090080920878	0.0
25	0.003822657368052619	0.0	0.0	11.53295727941475	0.0
26	0.003822657368052619	0.0	0.0	11.805082700802997	0.0
27	0.004061573453555908	0.0	0.0	12.076252457849229	0.0
28	0.005017237795569062	0.0	0.0	12.351244872263514	0.0
29	0.005017237795569062	0.0	0.0	12.692655958447714	0.0
30	0.005017237795569062	0.0	0.0	13.01997099558722	0.0
31	0.005017237795569062	0.0	0.0	13.25171959852541	0.0
32	0.005017237795569062	0.0	0.0	13.53005683813674	0.0
33	0.005017237795569062	0.0	0.0	13.805766000807536	0.0
34	0.005017237795569062	0.0	0.0	14.21431250701816	0.0
35	0.005017237795569062	0.0	0.0	14.498622648767073	0.0
36	0.005017237795569062	0.0	0.0	14.783649538772497	0.0
37	0.005256153881072351	0.0	0.0	15.069871009205437	0.0
38	0.005256153881072351	0.0	0.0	15.390257479865348	0.0
39	0.005256153881072351	0.0	0.0	15.703954300131166	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTAG	20	7.0281397E-4	45.000004	38
CTATGTG	20	7.0281397E-4	45.000004	16
CATGGGT	40	6.7975634E-9	45.000004	3
AGCGTGG	20	7.0281397E-4	45.000004	1
TGATATC	20	7.0281397E-4	45.000004	22
AACCGAT	20	7.0281397E-4	45.000004	32
GTACCCA	20	7.0281397E-4	45.000004	8
CGAACAT	20	7.0281397E-4	45.000004	12
GTCGACT	20	7.0281397E-4	45.000004	33
TTTACGG	20	7.0281397E-4	45.000004	1
ACTTGAC	20	7.0281397E-4	45.000004	34
TTTAACG	20	7.0281397E-4	45.000004	12
TTAGATT	20	7.0281397E-4	45.000004	12
CGTTGGT	40	6.7975634E-9	45.000004	32
TGTGGCA	20	7.0281397E-4	45.000004	19
ACATTGT	20	7.0281397E-4	45.000004	32
GTCTATG	20	7.0281397E-4	45.000004	14
CGTCTAT	20	7.0281397E-4	45.000004	13
GCTGATA	20	7.0281397E-4	45.000004	29
CCTAAGA	40	6.7975634E-9	45.000004	30
>>END_MODULE