##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043474_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1075401
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.481136803852703	31.0	31.0	33.0	30.0	34.0
2	30.515275697158547	31.0	31.0	34.0	26.0	34.0
3	31.397038872011464	31.0	31.0	34.0	28.0	34.0
4	35.0386776653546	37.0	35.0	37.0	32.0	37.0
5	35.34701195182076	37.0	35.0	37.0	33.0	37.0
6	35.79745694861731	37.0	35.0	37.0	35.0	37.0
7	33.7622858821965	37.0	35.0	37.0	32.0	37.0
8	34.76616164574889	37.0	35.0	37.0	31.0	37.0
9	36.72132906701779	39.0	37.0	39.0	32.0	39.0
10	36.69777971194001	39.0	35.0	39.0	32.0	39.0
11	36.48912731157959	39.0	35.0	39.0	32.0	39.0
12	36.42401764551084	39.0	35.0	39.0	32.0	39.0
13	36.465151138970484	39.0	35.0	39.0	32.0	39.0
14	37.826554001716566	40.0	36.0	41.0	33.0	41.0
15	37.9669202464941	40.0	36.0	41.0	33.0	41.0
16	37.938672179029034	40.0	36.0	41.0	33.0	41.0
17	37.89331421488357	40.0	36.0	41.0	33.0	41.0
18	37.5723186048739	39.0	36.0	41.0	33.0	41.0
19	37.23656942852015	38.0	35.0	41.0	33.0	41.0
20	36.890306034679156	38.0	35.0	41.0	32.0	41.0
21	36.78611141332396	38.0	35.0	40.0	32.0	41.0
22	36.67661923319766	38.0	35.0	40.0	32.0	41.0
23	36.5886232205475	37.0	35.0	40.0	32.0	41.0
24	36.37642144651158	37.0	35.0	40.0	32.0	41.0
25	36.22160942755307	36.0	35.0	40.0	32.0	41.0
26	35.918038015586745	36.0	35.0	40.0	31.0	41.0
27	35.824736075194274	36.0	35.0	40.0	31.0	41.0
28	35.699465594694445	36.0	35.0	40.0	31.0	41.0
29	35.62439964255194	36.0	35.0	40.0	31.0	41.0
30	35.41810450241352	35.0	35.0	40.0	30.0	41.0
31	34.956196804726794	35.0	34.0	40.0	27.0	41.0
32	34.76602123300982	35.0	34.0	40.0	25.0	41.0
33	34.61930944828952	35.0	34.0	40.0	25.0	41.0
34	34.433012429782	35.0	34.0	40.0	24.0	41.0
35	34.214621336599095	35.0	34.0	40.0	23.0	41.0
36	34.04715822283967	35.0	33.0	40.0	22.0	41.0
37	33.95774320462785	35.0	33.0	40.0	21.0	41.0
38	33.888867501518035	35.0	33.0	40.0	21.0	41.0
39	33.76920051218104	35.0	33.0	40.0	21.0	41.0
40	33.68765883609928	35.0	33.0	40.0	21.0	41.0
41	33.592558496783994	35.0	33.0	40.0	20.0	41.0
42	33.486069847433654	35.0	33.0	40.0	20.0	41.0
43	33.32489462070428	35.0	33.0	40.0	18.0	41.0
44	33.11605903286309	35.0	33.0	40.0	18.0	41.0
45	32.926626439811756	35.0	33.0	40.0	18.0	41.0
46	32.93759723117237	35.0	33.0	39.0	18.0	41.0
47	32.90366012306107	35.0	33.0	39.0	18.0	41.0
48	32.85115133796602	35.0	33.0	39.0	18.0	41.0
49	32.76464407230419	35.0	33.0	39.0	18.0	41.0
50	32.63636820125702	35.0	33.0	39.0	17.0	41.0
51	32.260677644897115	35.0	32.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	19.0
14	32.0
15	59.0
16	145.0
17	321.0
18	665.0
19	1239.0
20	2164.0
21	3410.0
22	5256.0
23	8169.0
24	12860.0
25	19080.0
26	23808.0
27	25896.0
28	26173.0
29	27184.0
30	29556.0
31	34638.0
32	42300.0
33	55218.0
34	90650.0
35	136468.0
36	141989.0
37	95249.0
38	116962.0
39	175783.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.410150260228512	18.14764910949497	24.857611253848564	32.58458937642796
2	33.52795840807289	19.933215609805085	28.71291732107372	17.8259086610483
3	20.88839418970226	20.906062017796152	40.96704392129076	17.238499871210834
4	21.391555336102535	24.156198478521034	37.84365087999732	16.608595305379108
5	18.556333869877374	30.299116329629598	35.46788593278228	15.676663867710742
6	74.83952497719454	2.222426797073836	21.306005852700526	1.6320423730310833
7	71.29284797019902	7.48074439209188	19.453766548478196	1.772641089230901
8	70.38351275477706	4.238604948293706	22.531130248158593	2.846752048770645
9	37.07900587780744	22.613425131648565	28.679999367677734	11.627569622866261
10	26.709943546639813	25.01615676384902	37.208352977168516	11.065546712342652
11	24.537916553918027	22.629233188364154	39.61220047219595	13.220649785521864
12	17.260073219199164	30.09705216937682	38.03557928623834	14.607295325185676
13	12.333352860932806	32.76182558878037	38.73476033591191	16.17006121437492
14	12.05466611989388	26.712919180845095	45.688724485099044	15.543690214161973
15	13.945030737371455	24.01011343675522	45.3017990498428	16.743056776030524
16	14.881890569192329	25.887645631722496	38.13442613499522	21.096037664089952
17	16.109897610286765	25.9957913373709	41.72173914660671	16.172571905735627
18	19.115846089040275	28.443622425495235	36.697938722392855	15.742592763071636
19	19.44084113739898	28.455989905160962	37.018098365167965	15.085070592272093
20	15.343299848149666	30.914514678710546	37.8642943422965	15.877891130843286
21	16.1925644480524	27.809161419786665	36.7886025770852	19.209671555075737
22	13.086188314870453	31.171721060330054	36.674133648750555	19.067956976048933
23	12.581632339936451	27.769455300859864	44.7923146807563	14.85659767844739
24	14.084606579313204	28.554185833935435	41.6483711657326	15.712836421018764
25	13.810476278151127	32.30720447535384	37.2992957975676	16.583023448927424
26	13.019422522389323	32.81566597018228	37.79148429283588	16.37342721459251
27	13.898257487207097	31.174789683104255	40.23885043811564	14.688102391573004
28	12.761844186494153	28.37536881591146	40.69923684281491	18.16355015477947
29	14.765003938065894	31.485650468987846	38.46518647462667	15.284159118319584
30	18.201861445172547	31.12931827290471	36.532512058292674	14.13630822363007
31	17.962229903078015	29.335382801392225	36.95430820689213	15.748079088637635
32	17.707999155663796	31.194875213989942	35.556225073251746	15.540900557094517
33	14.809545462576285	30.037539485271076	37.34058272216597	17.812332329986674
34	15.221112868595062	29.433671718735617	37.295855220517744	18.04936019215158
35	19.522485100906543	30.09128687810407	34.77735282001784	15.608875200971545
36	15.291133260988227	31.980442644185754	36.26154336847372	16.466880726352308
37	14.650349032593423	32.265545596479825	35.8167790433522	17.26732632757455
38	14.716370916523234	33.05752923793078	34.6122051216244	17.61389472392159
39	17.53364558894775	29.413865153556674	34.348117585905165	18.704371671590412
40	14.227158055460244	28.74602125160754	36.4502171748027	20.576603518129517
41	15.1736886984483	30.036795576719754	34.11527420934144	20.6742415154905
42	15.297177517967716	29.216543410318568	35.765263376173166	19.721015695540547
43	15.598274504115208	30.49839083281492	35.96146925658429	17.94186540648558
44	14.062661277049212	34.71040105039888	34.22686049203971	17.0000771805122
45	15.862826982679017	32.31138896095503	34.84997689234062	16.975807164025326
46	15.24807955358048	31.749087084724675	33.507221957204806	19.495611404490045
47	14.53439228715614	30.554276962732974	36.05473679120625	18.856593958904632
48	15.82228396663198	28.24127929953571	36.87136240341975	19.065074330412564
49	16.26323576042797	30.934228255320573	35.01140504797745	17.791130936274005
50	14.350088943566167	30.78089010517937	35.4948526177677	19.374168333486764
51	14.163739851460061	29.098912870640813	36.63647327833989	20.100873999559234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1246.0
1	8280.0
2	15314.0
3	59763.5
4	104213.0
5	60140.0
6	16067.0
7	16053.0
8	16039.0
9	20281.0
10	24523.0
11	27398.5
12	30274.0
13	29656.5
14	29039.0
15	25661.5
16	22284.0
17	18855.0
18	15426.0
19	13218.5
20	11011.0
21	10011.5
22	9012.0
23	8291.5
24	7571.0
25	7980.0
26	8749.0
27	9109.0
28	9954.5
29	10800.0
30	13160.5
31	15521.0
32	18047.5
33	20574.0
34	22900.5
35	25227.0
36	29413.0
37	33599.0
38	35599.0
39	37599.0
40	43091.0
41	48583.0
42	55087.5
43	61592.0
44	67181.0
45	72770.0
46	70340.5
47	67911.0
48	69238.0
49	70565.0
50	70547.0
51	70529.0
52	65885.0
53	61241.0
54	51497.5
55	41754.0
56	37337.5
57	32921.0
58	29055.5
59	25190.0
60	21219.5
61	17249.0
62	15061.0
63	12873.0
64	10880.5
65	8888.0
66	7256.5
67	5625.0
68	4931.5
69	4238.0
70	3793.5
71	3349.0
72	3229.5
73	3110.0
74	2608.0
75	1529.0
76	952.0
77	779.0
78	606.0
79	437.0
80	268.0
81	191.0
82	114.0
83	92.0
84	70.0
85	46.0
86	22.0
87	18.0
88	14.0
89	10.5
90	7.0
91	7.5
92	8.0
93	6.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1075401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.07771205770336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70715483858139	27.95944626366535
2	8.696420351064688	6.101010580147974
3	3.2328089170836183	3.401986209931049
4	1.74862357349885	2.4535085683401974
5	1.1181373178124332	1.961084943759864
6	0.8030499564414779	1.6901493084003232
7	0.578585505293595	1.4206819028814666
8	0.4178630498632908	1.1726143794126604
9	0.346949513016955	1.0953175604552234
>10	2.6365739813591613	20.078621316103515
>50	0.5588420002247294	13.500820255167682
>100	0.1473505945605398	7.749828210889274
>500	0.005184557956642697	1.1831586902316054
>1k	0.0019101002998157307	1.0529834763561112
>5k	2.728714714022472E-4	0.8648994357198273
>10k+	2.728714714022472E-4	8.313888898537902
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86859	8.076894107407377	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9036	0.8402447087179573	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3592	0.33401493954348194	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1574	0.1463640074725614	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1317	0.12246594526134902	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	1175	0.10926156847538733	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1162	0.10805271707948942	TruSeq Adapter, Index 13 (95% over 21bp)
CTGCAGTGGGATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATC	1103	0.10256639151349126	No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	1078	0.10024167729061066	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11093536271586134	0.0
2	0.0	0.0	0.0	0.35772702461686384	0.0
3	0.0	0.0	0.0	0.5836892470808563	0.0
4	0.0	0.0	0.0	0.7275425631927067	0.0
5	0.0	0.0	0.0	1.571506814667273	0.0
6	0.0	0.0	0.0	1.8241567564099346	0.0
7	0.0	0.0	0.0	2.509854463590791	0.0
8	0.0	0.0	0.0	3.3784606858278914	0.0
9	0.0	0.0	0.0	3.9999032918883284	0.0
10	0.0	0.0	0.0	4.445132559854417	0.0
11	0.0	0.0	0.0	4.926999323973104	0.0
12	0.0	0.0	0.0	5.319318096226431	0.0
13	0.0	0.0	0.0	5.487069474549494	0.0
14	0.0	0.0	0.0	5.617532436737552	0.0
15	0.0	0.0	0.0	6.054950664914762	0.0
16	0.0	0.0	0.0	6.602839312963257	0.0
17	0.0	0.0	0.0	7.224932839006101	0.0
18	0.0	0.0	0.0	7.546022367470367	0.0
19	0.0	0.0	0.0	7.9030984721048245	0.0
20	0.0	0.0	0.0	8.300159661372827	0.0
21	0.0	0.0	0.0	8.702056256224422	0.0
22	2.7896570674566974E-4	0.0	0.0	9.115855387897167	0.0
23	2.7896570674566974E-4	0.0	0.0	9.439083653446481	0.0
24	4.6494284457611627E-4	0.0	0.0	9.723442697189235	0.0
25	4.6494284457611627E-4	0.0	0.0	9.995341272697347	0.0
26	4.6494284457611627E-4	0.0	0.0	10.29643825884484	0.0
27	4.6494284457611627E-4	0.0	0.0	10.57112649142041	0.0
28	6.509199824065628E-4	0.0	0.0	10.87092163760309	0.0
29	0.0012088513958979023	0.0	0.0	11.224557165187683	0.0
30	0.0012088513958979023	0.0	0.0	11.549273247839643	0.0
31	0.0012088513958979023	0.0	0.0	11.846092759817036	0.0
32	0.0012088513958979023	0.0	0.0	12.155000785753407	0.0
33	0.0012088513958979023	0.0	0.0	12.464001800258695	0.0
34	0.0012088513958979023	0.0	0.0	12.826006299045659	0.0
35	0.0012088513958979023	0.0	0.0	13.124871559539185	0.0
36	0.0012088513958979023	0.0	0.0	13.444008328056231	0.0
37	0.0012088513958979023	0.0	0.0	13.792250518643742	0.0
38	0.0012088513958979023	0.0	0.0	14.134448452251764	0.0
39	0.0012088513958979023	0.0	0.0	14.49450019109151	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGG	40	6.8139343E-9	45.000004	1
ATTGTCG	20	7.0330827E-4	45.000004	10
ATTCGTT	20	7.0330827E-4	45.000004	43
CAATTCG	20	7.0330827E-4	45.000004	17
CCGTCGG	40	6.8139343E-9	45.000004	26
AGACGCA	20	7.0330827E-4	45.000004	21
GCCGATG	40	6.8139343E-9	45.000004	8
ACGCTTA	20	7.0330827E-4	45.000004	32
GCGATCG	20	7.0330827E-4	45.000004	8
CCGAATA	20	7.0330827E-4	45.000004	24
ATACGAG	20	7.0330827E-4	45.000004	2
TTCGCAT	20	7.0330827E-4	45.000004	27
AACAGCG	20	7.0330827E-4	45.000004	38
TCGGTCA	20	7.0330827E-4	45.000004	10
AGTACGG	40	6.8139343E-9	45.000004	1
CGCTTGT	20	7.0330827E-4	45.000004	32
ACGGTAC	20	7.0330827E-4	45.000004	37
CAGCGTA	25	3.890637E-5	45.0	35
ACCGGCC	35	1.211829E-7	45.0	20
GTCGATT	25	3.890637E-5	45.0	8
>>END_MODULE