##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043473_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	752621
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53639879833276	31.0	31.0	33.0	30.0	34.0
2	30.59889506139212	31.0	31.0	34.0	26.0	34.0
3	31.460955779867955	31.0	31.0	34.0	28.0	34.0
4	35.089466012774025	37.0	35.0	37.0	32.0	37.0
5	35.39457841330497	37.0	35.0	37.0	33.0	37.0
6	35.80245701355663	37.0	35.0	37.0	35.0	37.0
7	33.80585181651854	37.0	35.0	37.0	32.0	37.0
8	34.810839718796046	37.0	35.0	37.0	32.0	37.0
9	36.7922792481209	39.0	37.0	39.0	32.0	39.0
10	36.72423171822205	39.0	35.0	39.0	32.0	39.0
11	36.367377471529494	38.0	35.0	39.0	32.0	39.0
12	36.17494861291407	39.0	35.0	39.0	32.0	39.0
13	36.268613286102834	39.0	35.0	39.0	32.0	39.0
14	37.65711028525646	40.0	35.0	41.0	33.0	41.0
15	37.827727368755326	40.0	35.0	41.0	33.0	41.0
16	37.80003082560811	40.0	35.0	41.0	33.0	41.0
17	37.79970263917696	40.0	36.0	41.0	33.0	41.0
18	37.506858033459075	39.0	36.0	41.0	33.0	41.0
19	37.185459879540964	38.0	35.0	41.0	33.0	41.0
20	36.84634231572066	38.0	35.0	41.0	33.0	41.0
21	36.74031551072851	38.0	35.0	40.0	32.0	41.0
22	36.65534445624026	37.0	35.0	40.0	32.0	41.0
23	36.52903652701692	37.0	35.0	40.0	32.0	41.0
24	36.33855154187832	37.0	35.0	40.0	32.0	41.0
25	36.18494036174914	36.0	35.0	40.0	32.0	41.0
26	35.9215102953545	35.0	35.0	40.0	31.0	41.0
27	35.85790856221126	36.0	35.0	40.0	31.0	41.0
28	35.750410897383944	35.0	35.0	40.0	31.0	41.0
29	35.68970039369085	35.0	35.0	40.0	31.0	41.0
30	35.52689733610941	35.0	35.0	40.0	31.0	41.0
31	35.06778710665793	35.0	34.0	40.0	29.0	41.0
32	34.934271034159295	35.0	34.0	40.0	28.0	41.0
33	34.88462320344503	35.0	34.0	40.0	27.0	41.0
34	34.74680749009129	35.0	34.0	40.0	27.0	41.0
35	34.555603683660166	35.0	34.0	40.0	25.0	41.0
36	34.41836196438845	35.0	34.0	40.0	24.0	41.0
37	34.37028065918968	35.0	34.0	40.0	24.0	41.0
38	34.29596038377882	35.0	34.0	40.0	24.0	41.0
39	34.166556606844615	35.0	34.0	40.0	23.0	41.0
40	34.11102002202968	35.0	34.0	40.0	23.0	41.0
41	34.055607005385184	35.0	34.0	40.0	23.0	41.0
42	33.955500843053805	35.0	33.0	40.0	23.0	41.0
43	33.79696952383736	35.0	33.0	40.0	22.0	41.0
44	33.601596288171606	35.0	33.0	40.0	21.0	41.0
45	33.4543867364849	35.0	33.0	39.0	21.0	41.0
46	33.46815196493321	35.0	33.0	39.0	22.0	41.0
47	33.466184175036304	35.0	33.0	39.0	22.0	41.0
48	33.43135123787404	35.0	33.0	39.0	22.0	41.0
49	33.34057646544542	35.0	33.0	39.0	20.0	41.0
50	33.20611436566346	35.0	33.0	39.0	20.0	41.0
51	32.85539600941244	35.0	33.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	5.0
13	8.0
14	24.0
15	39.0
16	95.0
17	188.0
18	421.0
19	751.0
20	1324.0
21	2022.0
22	3195.0
23	4926.0
24	7607.0
25	11040.0
26	13783.0
27	15330.0
28	16202.0
29	17152.0
30	19582.0
31	23366.0
32	29109.0
33	39216.0
34	71346.0
35	111631.0
36	100285.0
37	63130.0
38	76343.0
39	124417.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.069855876995195	18.033246481296693	25.316859348862174	31.580038292845934
2	32.3573219455742	20.58220538624354	28.696116637723367	18.36435603045889
3	21.20894846144341	21.633996393935327	39.07012958713615	18.08692555748511
4	21.373706022021707	24.503435328007058	36.991128336838855	17.131730313132373
5	19.06643582892319	31.187144658466874	34.08050001262256	15.665919499987377
6	77.22891070007347	1.836515324446169	19.353831476931948	1.5807424985484062
7	73.82161805211388	7.171737169172797	17.321334376797886	1.6853104019154395
8	73.33704480741304	3.9373070908199477	20.26411699912705	2.461531102639974
9	40.93879920969519	22.581883843262414	25.95967957311848	10.519637373923928
10	30.773257722014137	24.005043707257702	34.55351365428283	10.668184916445329
11	28.368328813572834	23.1694305633247	35.28761488185953	13.174625741242938
12	16.114086638560444	36.108213828739835	34.43751901687569	13.340180515824034
13	11.090841206928854	38.46132382699924	35.77351681656504	14.674318149506856
14	10.408558889534042	27.504414572540497	47.05927684717806	15.027749690747402
15	11.564120586590063	24.5739887672547	47.31491680407536	16.54697384207988
16	12.244675606978811	26.09188422858251	35.97547769727393	25.68796246716475
17	14.72839583269667	27.394664778155274	40.18676066705553	17.69017872209253
18	19.044645312846704	29.385042405141498	35.46884819849566	16.10146408351614
19	20.71799750472017	28.693592126714506	34.557101117295424	16.031309251269896
20	14.947098207464315	33.178186630455436	35.224103499636605	16.650611662443644
21	15.585400885704756	29.679081503173578	33.57161174083635	21.16390587028531
22	12.279221547100068	33.834028016757436	33.7188305933531	20.1679198427894
23	11.171891297213339	28.836559171216326	45.702285745415026	14.289263786155315
24	12.907160443304132	29.27422965875255	41.999093833416815	15.819516064526502
25	11.199926656311742	36.72167000389307	35.218523001617015	16.85988033817818
26	11.51921086443243	36.67131265271631	34.78470571509432	17.024770767756944
27	12.06211360033802	32.48700209002938	40.63931248264399	14.811571826988617
28	10.7710255228063	29.653437786083565	40.37463743371498	19.200899257395157
29	12.298088945166292	34.79785974614049	36.6132488995125	16.290802409180717
30	19.232389210505684	33.22163479360794	32.98765248378666	14.558323512099717
31	19.65252098998035	31.266334582744836	33.36300741010415	15.71813701717066
32	17.546680201588845	32.93357480059685	32.58412932937029	16.93561566844401
33	13.816781620496904	33.055681412025436	32.8353846092522	20.292152358225454
34	13.95602833298566	32.57549284433998	35.45702285745415	18.01145596522021
35	20.94865808952979	31.684074720211104	31.86570664384863	15.501560546410479
36	14.30919413622527	37.07536728313454	33.48325385552622	15.132184725113968
37	14.978322422573912	36.30778306744032	33.31982498495258	15.394069525033183
38	14.467573984781184	36.964421667745114	32.3873503396796	16.180654007794097
39	18.268424612122168	32.226445980114825	32.26152339623795	17.243606011525056
40	12.58335868916759	31.345391638022324	34.526541247188156	21.544708425621927
41	15.366432772936179	31.80564985563783	31.614849970968123	21.213067400457867
42	15.348761195874152	31.306461020885678	32.93875669161503	20.406021091625135
43	15.030805677758128	32.91298010552456	34.54540864525438	17.510805571462928
44	11.879684462697693	39.72251637942603	30.57515004231878	17.8226491155575
45	15.641471603901566	36.35853902561847	31.722340992345416	16.277648378134547
46	15.102422069009503	34.32856643649327	31.936658690097673	18.632352804399556
47	14.622499239324974	32.590507041392684	34.841839385294854	17.945154333987492
48	15.259207489559817	30.911308613498694	36.02291193044042	17.806571966501068
49	15.449608767228126	34.48734489205058	32.54293993922572	17.520106401495575
50	13.338320349817504	34.310894859431244	32.83551747825267	19.515267312498587
51	13.742640718236668	30.358174964557193	35.87981201693814	20.019372300267996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	876.0
1	5491.5
2	10107.0
3	40721.5
4	71336.0
5	40301.0
6	9266.0
7	9639.5
8	10013.0
9	13389.5
10	16766.0
11	19486.5
12	22207.0
13	21617.0
14	21027.0
15	18248.0
16	15469.0
17	12970.0
18	10471.0
19	8871.5
20	7272.0
21	6498.5
22	5725.0
23	5269.5
24	4814.0
25	4758.5
26	5284.5
27	5866.0
28	6720.0
29	7574.0
30	9823.0
31	12072.0
32	13170.5
33	14269.0
34	17315.5
35	20362.0
36	21796.5
37	23231.0
38	23964.0
39	24697.0
40	31199.5
41	37702.0
42	44901.0
43	52100.0
44	55021.5
45	57943.0
46	56539.5
47	55136.0
48	54384.0
49	53632.0
50	49714.5
51	45797.0
52	39543.5
53	33290.0
54	28707.0
55	24124.0
56	22531.5
57	20939.0
58	19286.5
59	17634.0
60	14298.0
61	10962.0
62	9241.5
63	7521.0
64	6473.5
65	5426.0
66	4276.5
67	3127.0
68	2737.5
69	2348.0
70	2137.5
71	1927.0
72	2016.5
73	2106.0
74	1830.5
75	1115.0
76	675.0
77	448.5
78	222.0
79	172.0
80	122.0
81	101.0
82	80.0
83	74.5
84	69.0
85	42.0
86	15.0
87	16.0
88	17.0
89	13.5
90	10.0
91	9.5
92	9.0
93	9.5
94	10.0
95	5.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	752621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.46335744044239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.84091977478843	24.49136682366969
2	9.212819929110701	5.797324929280823
3	3.6590250223231653	3.453756364826294
4	2.0260773649590997	2.5498878534279124
5	1.2660185237531045	1.991659666953257
6	0.9351439202977982	1.7653660453551718
7	0.5917875122551028	1.3033735418810741
8	0.48909772093121373	1.2310925133571615
9	0.36988998304673515	1.0474182675214743
>10	2.6250706866478035	17.814223951628456
>50	0.7127332606305239	15.955681596915703
>100	0.2658945298692875	12.012733745161817
>500	0.0025485098709516377	0.5626291503552338
>1k	0.0021237582257930313	0.989478914306449
>5k	4.247516451586063E-4	0.8329851530081169
>10k+	4.247516451586063E-4	8.201021482351372
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61366	8.153639082619273	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6233	0.8281724799068854	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2498	0.33190676316499274	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1592	0.21152744874246135	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1161	0.1542609095414558	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1078	0.14323278250274707	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	1075	0.14283417550134794	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	799	0.10616233137262979	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	779	0.10350495136330239	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	766	0.10177765435723957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11320438839734741	0.0
2	0.0	0.0	0.0	0.3655226202829844	0.0
3	0.0	0.0	0.0	0.5953859910898048	0.0
4	0.0	0.0	0.0	0.7455279616168031	0.0
5	0.0	0.0	0.0	1.6342887057363533	0.0
6	0.0	0.0	0.0	1.9131807377152643	0.0
7	0.0	0.0	0.0	2.6361209692527847	0.0
8	0.0	0.0	0.0	3.4848881442319573	0.0
9	0.0	0.0	0.0	4.106847935415036	0.0
10	0.0	0.0	0.0	4.502133211802487	0.0
11	1.328690004663702E-4	0.0	0.0	4.963720119422658	0.0
12	1.328690004663702E-4	0.0	0.0	5.343991198757409	0.0
13	1.328690004663702E-4	0.0	0.0	5.500909488308192	0.0
14	1.328690004663702E-4	0.0	0.0	5.623813313739585	0.0
15	1.328690004663702E-4	0.0	0.0	6.015378258113977	0.0
16	1.328690004663702E-4	0.0	0.0	6.492909445790112	0.0
17	1.328690004663702E-4	0.0	0.0	7.0958689699065	0.0
18	1.328690004663702E-4	0.0	0.0	7.348054332791671	0.0
19	1.328690004663702E-4	0.0	0.0	7.641030478820017	0.0
20	1.328690004663702E-4	0.0	0.0	7.950748118907126	0.0
21	2.657380009327404E-4	0.0	0.0	8.254486653973249	0.0
22	2.657380009327404E-4	0.0	0.0	8.574435207096268	0.0
23	2.657380009327404E-4	0.0	0.0	8.826089093979572	0.0
24	0.0010629520037309615	0.0	0.0	9.025259725678662	0.0
25	0.0010629520037309615	0.0	0.0	9.223101667373086	0.0
26	0.0010629520037309615	0.0	0.0	9.45137061017431	0.0
27	0.0010629520037309615	0.0	0.0	9.655988870892521	0.0
28	0.0010629520037309615	0.0	0.0	9.872166734651305	0.0
29	0.0010629520037309615	0.0	0.0	10.162751238671257	0.0
30	0.0010629520037309615	0.0	0.0	10.408027413532176	0.0
31	0.0010629520037309615	0.0	0.0	10.613708626254118	0.0
32	0.0010629520037309615	0.0	0.0	10.828956407009636	0.0
33	0.0010629520037309615	0.0	0.0	11.047924519778215	0.0
34	0.0010629520037309615	0.0	0.0	11.319774494732409	0.0
35	0.0010629520037309615	0.0	0.0	11.544721712521973	0.0
36	0.0010629520037309615	0.0	0.0	11.771661965318534	0.0
37	0.0010629520037309615	0.0	0.0	12.013882153168726	0.0
38	0.0010629520037309615	0.0	0.0	12.257696769024516	0.0
39	0.0010629520037309615	0.0	0.0	12.50536458589383	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	35	1.2111741E-7	45.000004	1
AAGTAGG	100	0.0	45.000004	1
TCTTGAC	35	1.2111741E-7	45.000004	28
CGTATTT	25	3.889517E-5	45.000004	1
GGTCGTA	25	3.889517E-5	45.000004	8
ACGTTGG	30	2.1644992E-6	45.000004	1
CTATCCG	25	3.889517E-5	45.000004	15
CCTAGAC	25	3.889517E-5	45.000004	41
ACGCAGT	25	3.889517E-5	45.000004	13
TAGACGG	35	1.2111741E-7	45.000004	1
CCGGCTA	25	3.889517E-5	45.000004	11
ACCGCGT	25	3.889517E-5	45.000004	34
GACCGAT	100	0.0	45.000004	8
GTGATTA	200	0.0	45.000004	43
CACGAGA	30	2.1644992E-6	45.000004	3
TCGATGG	35	1.2111741E-7	45.000004	1
GCACCGG	30	2.1644992E-6	45.000004	1
AGTATGC	25	3.889517E-5	45.000004	7
ATCGTCG	30	2.1644992E-6	45.000004	32
AGTATCT	35	1.2111741E-7	45.000004	24
>>END_MODULE