##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043472_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3166475
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.498200680567507	31.0	31.0	33.0	30.0	34.0
2	30.53011124357527	31.0	31.0	34.0	26.0	34.0
3	31.494633306752778	31.0	31.0	34.0	28.0	34.0
4	35.11075596680852	37.0	35.0	37.0	32.0	37.0
5	35.42221113383179	37.0	35.0	37.0	33.0	37.0
6	35.748364348368455	37.0	35.0	37.0	35.0	37.0
7	33.71604007610987	37.0	35.0	37.0	32.0	37.0
8	34.707628514357445	37.0	35.0	37.0	31.0	37.0
9	36.73188545622498	39.0	37.0	39.0	32.0	39.0
10	36.658467538824716	39.0	35.0	39.0	32.0	39.0
11	36.18864289154344	38.0	35.0	39.0	32.0	39.0
12	36.121797266676666	38.0	35.0	39.0	32.0	39.0
13	36.1194056482366	38.0	35.0	39.0	32.0	39.0
14	37.244928508830796	39.0	35.0	41.0	32.0	41.0
15	37.30301834058377	39.0	35.0	41.0	32.0	41.0
16	37.016026022627685	39.0	35.0	41.0	31.0	41.0
17	36.94894196227666	39.0	35.0	41.0	31.0	41.0
18	36.84869484205623	39.0	35.0	40.0	31.0	41.0
19	36.73508965016303	38.0	35.0	40.0	31.0	41.0
20	36.53221547619987	38.0	35.0	40.0	31.0	41.0
21	36.443205772980996	38.0	35.0	40.0	31.0	41.0
22	36.39965892672451	37.0	35.0	40.0	31.0	41.0
23	36.38569576579635	37.0	35.0	40.0	31.0	41.0
24	36.29039547130484	37.0	35.0	40.0	31.0	41.0
25	36.16520957847449	37.0	35.0	40.0	31.0	41.0
26	35.88979732983839	36.0	35.0	40.0	30.0	41.0
27	35.77886166794306	36.0	35.0	40.0	30.0	41.0
28	35.61961676627796	36.0	34.0	40.0	30.0	41.0
29	35.42756819491706	36.0	34.0	40.0	29.0	41.0
30	35.25404084984091	36.0	34.0	40.0	29.0	41.0
31	35.108384244309526	36.0	34.0	40.0	28.0	41.0
32	35.02778041828847	36.0	34.0	40.0	27.0	41.0
33	34.71226521605255	36.0	33.0	40.0	25.0	41.0
34	34.61291751869192	36.0	33.0	40.0	24.0	41.0
35	34.55422133444919	36.0	34.0	40.0	24.0	41.0
36	34.523226616347834	36.0	34.0	40.0	24.0	41.0
37	34.437893872523865	35.0	34.0	40.0	24.0	41.0
38	34.33807625198367	35.0	34.0	40.0	23.0	41.0
39	34.22945862512731	35.0	34.0	40.0	23.0	41.0
40	34.15450366732723	35.0	34.0	40.0	23.0	41.0
41	34.07073133373862	35.0	33.0	40.0	23.0	41.0
42	33.95231321895799	35.0	33.0	40.0	23.0	41.0
43	33.76897212199686	35.0	33.0	40.0	23.0	41.0
44	33.53406516710222	35.0	33.0	40.0	22.0	41.0
45	33.31396268721528	35.0	33.0	39.0	20.0	41.0
46	33.31999684191412	35.0	33.0	39.0	21.0	41.0
47	33.31579564026244	35.0	33.0	39.0	22.0	41.0
48	33.22710995665527	35.0	33.0	39.0	22.0	41.0
49	33.1927354550407	35.0	33.0	39.0	22.0	41.0
50	33.14332688557465	35.0	33.0	39.0	22.0	41.0
51	32.732219581711526	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	12.0
12	21.0
13	45.0
14	87.0
15	186.0
16	429.0
17	971.0
18	1980.0
19	3707.0
20	5820.0
21	9463.0
22	14171.0
23	20896.0
24	30785.0
25	44718.0
26	58443.0
27	70787.0
28	79981.0
29	88033.0
30	99755.0
31	115860.0
32	138929.0
33	183155.0
34	355459.0
35	343476.0
36	292122.0
37	317078.0
38	394658.0
39	495202.0
40	240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.754379870360577	17.516544422425568	24.26344752445543	28.46562818275843
2	31.203246512288903	20.93078265263424	28.240046108053907	19.62592472702295
3	26.51402584893296	21.304794763893604	33.13141584885401	19.049763538319425
4	24.145208788953017	24.581245706977	29.393315911226203	21.88022959284378
5	21.404274469244193	32.938109411885456	28.45688028485935	17.200735834011006
6	82.08042065703977	2.358521699997631	14.106790674172384	1.454266968790216
7	78.07928374612148	7.323885393063264	13.001839585027513	1.5949912757877451
8	77.11341475931438	3.590301518249789	16.184432215634104	3.1118515068017274
9	48.63692276111449	16.810333257012925	21.193535398195156	13.359208583677434
10	36.76318303476263	16.52998997307732	28.036412730244198	18.67041426191585
11	33.321753685091466	14.362027175329034	34.81192809038442	17.504291049195082
12	25.42154919903047	16.732865410274833	35.57432160367601	22.27126378701869
13	19.264008084699864	19.295746847835527	41.07141221705524	20.368832850409365
14	22.700889790697858	17.01952486597873	41.23237985457015	19.047205488753267
15	29.57793761201336	14.353310858288793	37.68180705674291	18.38694447295494
16	26.088473776044342	16.173536819333805	35.695939491074455	22.042049913547398
17	26.486392597446688	18.940525347586828	35.43896604268153	19.134116012284956
18	26.473286541027484	24.937698860720516	31.030688699579184	17.558325898672813
19	29.12756298407535	22.893027735889277	29.390726280801204	18.588682999234162
20	27.917700281859165	25.538745766191113	31.278377375472726	15.265176576476996
21	22.379017677385736	20.750519110367208	36.474376080657514	20.396087131589542
22	26.12753929843122	19.39664769183398	35.096566371122464	19.379246638612337
23	30.040060319440386	18.098548070014765	33.6834808422615	18.177910768283343
24	28.144956142082282	16.009916389676217	31.046100158693818	24.799027309547682
25	20.158567492242952	18.475876171452484	35.559699666032415	25.80585667027215
26	17.934832897780655	18.723091134463402	36.75377193882788	26.588304028928068
27	19.587427660095216	23.925563915710686	33.864502325140734	22.622506099053364
28	25.146479918521386	24.044181621519197	30.888069541051166	19.92126891890825
29	24.50093558294318	20.090731807451505	36.49165081044379	18.916681799161527
30	30.91071301684049	18.410819602239084	32.739276324619645	17.93919105630078
31	32.1886956315777	19.333707040162956	30.12558128518305	18.35201604307629
32	31.434639465020254	19.327106640665093	29.43462367459083	19.803630219723825
33	23.770944030822918	21.180176694905217	35.60849209294247	19.440387181329395
34	21.53764043613166	25.593980688304818	33.30687910057714	19.56149977498638
35	23.167560141798056	30.804001294815215	27.825736820912844	18.202701742473888
36	25.99114156909497	27.511980988322975	26.472023306673826	20.024854135908228
37	24.701758264316	31.045121152069733	25.46636243772649	18.786758145887777
38	25.908115491200785	25.824931509012387	25.746295170497163	22.520657829289668
39	24.166241640941426	20.9993131163202	29.31556383675854	25.518881405979837
40	20.161946644138986	23.878540016895762	29.61466615084597	26.34484718811928
41	19.223900393971213	25.876913602665425	26.689141711208837	28.210044292154524
42	19.003623903552057	19.90197301415612	29.291815030909767	31.80258805138206
43	20.551180729359935	19.434481560726045	31.505538493119325	28.5087992167947
44	24.128091963460946	22.428378559754933	29.70416630480266	23.739363171981463
45	23.951397058243	23.876297775918015	30.691447113904264	21.480858051934725
46	20.422930861604783	23.73759464388634	30.447737626224747	25.39173686828413
47	19.966271642757324	23.114630622379774	33.92336904602121	22.995728688841695
48	24.765993731199522	21.667690412840777	32.3659589922548	21.200356863704908
49	21.473373388389298	20.17142090179142	33.96319250902028	24.392013200798996
50	18.672277532587497	21.38276790437316	36.447248122912704	23.49770644012664
51	18.262800116849178	18.917060769467625	35.5229711271998	27.297167986483394
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1783.0
1	15710.0
2	29637.0
3	102357.0
4	175077.0
5	104868.0
6	34659.0
7	29551.5
8	24444.0
9	23856.5
10	23269.0
11	22782.5
12	22296.0
13	21221.0
14	20146.0
15	18721.5
16	17297.0
17	15990.5
18	14684.0
19	14024.5
20	13365.0
21	13084.0
22	12803.0
23	12482.5
24	12162.0
25	12450.5
26	15658.0
27	18577.0
28	20181.0
29	21785.0
30	24134.0
31	26483.0
32	30149.5
33	33816.0
34	39428.5
35	45041.0
36	49041.0
37	53041.0
38	56465.5
39	59890.0
40	65887.5
41	71885.0
42	82795.5
43	93706.0
44	102299.0
45	110892.0
46	119452.0
47	128012.0
48	150381.5
49	172751.0
50	174474.5
51	176198.0
52	167768.0
53	159338.0
54	167991.0
55	176644.0
56	192869.5
57	209095.0
58	220064.5
59	231034.0
60	236594.0
61	242154.0
62	233210.5
63	224267.0
64	199689.0
65	175111.0
66	144108.5
67	113106.0
68	90349.5
69	67593.0
70	57790.0
71	47987.0
72	43985.5
73	39984.0
74	33568.0
75	20416.0
76	13680.0
77	10327.0
78	6974.0
79	5231.0
80	3488.0
81	2454.0
82	1420.0
83	985.0
84	550.0
85	406.5
86	263.0
87	181.0
88	99.0
89	74.5
90	50.0
91	35.0
92	20.0
93	17.5
94	15.0
95	11.5
96	8.0
97	6.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3166475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.11812500277322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24633796311448	19.40283172963976
2	9.336198013468612	4.690155775058953
3	3.135429256744597	2.3626831202478935
4	1.4310979277628573	1.4378598656302868
5	0.8224671806483865	1.0329416727102316
6	0.5846478083565324	0.8811154039738068
7	0.4615087718058857	0.8114564514067604
8	0.36279560184223414	0.7290196222023664
9	0.3128930295034154	0.7073357604806878
>10	4.843190343979679	30.189758991347443
>50	1.2111755600245144	20.53967230384182
>100	0.24322731690169805	9.394349194607198
>500	0.005391776625169041	0.9976548133142535
>1k	0.0028306827282137464	1.3888143211612491
>5k	4.043832468876781E-4	0.7200883244949279
>10k+	4.043832468876781E-4	4.714262649882466
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105513	3.3321911589385675	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	22061	0.6967053269013651	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11663	0.36832755666790357	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8805	0.2780694620990218	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7407	0.233919421438666	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5056	0.1596728223024025	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4714	0.1488721685786245	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4591	0.14498772294112539	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3558	0.11236469575790113	No Hit
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG	3214	0.10150088031644021	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04699231795608681	0.0
2	0.0	0.0	0.0	0.14985117520270963	0.0
3	0.0	0.0	0.0	0.22555049384568013	0.0
4	0.0	0.0	0.0	0.2770272937572537	0.0
5	0.0	0.0	0.0	0.5955518360321809	0.0
6	0.0	0.0	0.0	0.6938314687467926	0.0
7	0.0	0.0	0.0	0.9920810996455048	0.0
8	0.0	0.0	0.0	1.3632509336091394	0.0
9	0.0	0.0	0.0	1.685944149251139	0.0
10	0.0	0.0	0.0	1.91086302591999	0.0
11	0.0	0.0	0.0	2.1365714240598774	0.0
12	0.0	0.0	0.0	2.324161725578127	0.0
13	6.316171768291238E-5	0.0	0.0	2.4204201833268857	0.0
14	6.316171768291238E-5	0.0	0.0	2.492235056332357	0.0
15	6.316171768291238E-5	0.0	0.0	2.693184061140543	0.0
16	6.316171768291238E-5	0.0	0.0	2.9167449608792113	3.158085884145619E-5
17	6.316171768291238E-5	0.0	0.0	3.204793974372133	3.158085884145619E-5
18	6.316171768291238E-5	0.0	0.0	3.386320750992823	3.158085884145619E-5
19	6.316171768291238E-5	0.0	0.0	3.59835463725436	3.158085884145619E-5
20	6.316171768291238E-5	0.0	0.0	3.8264000189485152	3.158085884145619E-5
21	6.316171768291238E-5	0.0	0.0	4.052929519418281	3.158085884145619E-5
22	6.316171768291238E-5	0.0	0.0	4.2679320064109145	3.158085884145619E-5
23	6.316171768291238E-5	0.0	0.0	4.482681846532817	3.158085884145619E-5
24	1.2632343536582476E-4	0.0	0.0	4.671314316392834	3.158085884145619E-5
25	1.2632343536582476E-4	0.0	0.0	4.860041528829377	3.158085884145619E-5
26	1.2632343536582476E-4	0.0	0.0	5.047505506912261	3.158085884145619E-5
27	1.2632343536582476E-4	0.0	0.0	5.264308102858857	3.158085884145619E-5
28	1.2632343536582476E-4	0.0	0.0	5.47820525979204	3.158085884145619E-5
29	1.2632343536582476E-4	0.0	0.0	5.700913476341989	3.158085884145619E-5
30	1.2632343536582476E-4	0.0	0.0	5.923969082339195	3.158085884145619E-5
31	1.2632343536582476E-4	0.0	0.0	6.154319866728776	3.158085884145619E-5
32	1.2632343536582476E-4	0.0	0.0	6.435421091276577	3.158085884145619E-5
33	1.2632343536582476E-4	0.0	0.0	6.70332151682865	3.158085884145619E-5
34	1.2632343536582476E-4	0.0	0.0	6.984106932788037	3.158085884145619E-5
35	1.2632343536582476E-4	0.0	0.0	7.250902028280659	3.158085884145619E-5
36	1.2632343536582476E-4	0.0	0.0	7.532445384852241	3.158085884145619E-5
37	1.8948515304873716E-4	0.0	0.0	7.956639480810681	3.158085884145619E-5
38	1.8948515304873716E-4	0.0	0.0	8.384023243512107	3.158085884145619E-5
39	1.8948515304873716E-4	0.0	0.0	8.943793966476917	3.158085884145619E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAC	20	7.0351636E-4	45.000004	45
TATACGG	225	0.0	45.0	1
CGTTAGA	30	2.1667147E-6	44.999996	29
CGTTTTT	28800	0.0	44.015625	1
ATAGCGG	440	0.0	42.954544	1
GCGATCG	105	0.0	42.857143	8
ATTACGG	225	0.0	42.0	1
CACTTAC	7235	0.0	40.863857	36
CATGCGG	550	0.0	40.5	1
GCGCGAC	3490	0.0	40.422634	8
GCACTTA	7350	0.0	40.010204	35
ACTTACT	7320	0.0	39.95902	37
CGCACTT	7380	0.0	39.939022	34
CTTACTG	7315	0.0	39.924812	38
CATATGC	1270	0.0	39.862206	32
ACCCGCA	7445	0.0	39.74144	31
CCGCACT	7440	0.0	39.70766	33
AATACGG	85	0.0	39.705883	1
TATGCGG	550	0.0	39.68182	1
CCCGCAC	7525	0.0	39.52824	32
>>END_MODULE