##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043471_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2473330
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49950795081934	31.0	31.0	33.0	30.0	34.0
2	30.65552716378324	31.0	31.0	34.0	26.0	34.0
3	31.566849551010176	31.0	31.0	34.0	28.0	34.0
4	35.195707002300544	37.0	35.0	37.0	32.0	37.0
5	35.500032749370284	37.0	35.0	37.0	33.0	37.0
6	35.72072671256969	37.0	35.0	37.0	35.0	37.0
7	34.03670597938811	37.0	35.0	37.0	32.0	37.0
8	34.86966033646946	37.0	35.0	37.0	32.0	37.0
9	36.80509677236762	39.0	37.0	39.0	32.0	39.0
10	36.703750813680344	39.0	35.0	39.0	32.0	39.0
11	36.45419212155272	39.0	35.0	39.0	32.0	39.0
12	36.45076354550343	39.0	35.0	39.0	32.0	39.0
13	36.483596608620765	39.0	35.0	39.0	32.0	39.0
14	37.678115738700455	40.0	36.0	41.0	33.0	41.0
15	37.76703108764298	40.0	36.0	41.0	33.0	41.0
16	37.57249335915547	40.0	35.0	41.0	32.0	41.0
17	37.55363942539006	40.0	36.0	41.0	32.0	41.0
18	37.40600930729017	39.0	35.0	41.0	32.0	41.0
19	37.25842245070411	39.0	35.0	41.0	32.0	41.0
20	37.041534692095276	39.0	35.0	41.0	32.0	41.0
21	36.94659143745477	38.0	35.0	41.0	32.0	41.0
22	36.88648583084344	38.0	35.0	41.0	32.0	41.0
23	36.829283597417245	38.0	35.0	40.0	32.0	41.0
24	36.69674406569281	38.0	35.0	40.0	31.0	41.0
25	36.59698544068119	38.0	35.0	40.0	31.0	41.0
26	36.346264752378374	38.0	35.0	40.0	31.0	41.0
27	36.23969344972163	38.0	35.0	40.0	31.0	41.0
28	36.035597756870295	38.0	35.0	40.0	30.0	41.0
29	35.94399493799857	38.0	35.0	40.0	30.0	41.0
30	35.79933935220937	38.0	34.0	40.0	30.0	41.0
31	35.680385553080264	38.0	34.0	40.0	29.0	41.0
32	35.591931929827396	38.0	34.0	40.0	29.0	41.0
33	35.32294760505068	37.0	34.0	40.0	27.0	41.0
34	35.252276485547824	37.0	34.0	40.0	27.0	41.0
35	35.12017967679202	37.0	34.0	40.0	26.0	41.0
36	35.018460132695594	37.0	34.0	40.0	26.0	41.0
37	34.896759833908135	37.0	34.0	40.0	25.0	41.0
38	34.76339631185487	37.0	34.0	40.0	25.0	41.0
39	34.63431163653859	36.0	34.0	40.0	24.0	41.0
40	34.52758346035507	36.0	34.0	40.0	24.0	41.0
41	34.422869572600504	36.0	34.0	40.0	24.0	41.0
42	34.295975061961	35.0	33.0	40.0	23.0	41.0
43	34.128554216380344	35.0	33.0	40.0	23.0	41.0
44	33.89551171901849	35.0	33.0	40.0	23.0	41.0
45	33.688750389151465	35.0	33.0	40.0	23.0	41.0
46	33.65599940161644	35.0	33.0	39.0	23.0	41.0
47	33.606208633704355	35.0	33.0	39.0	23.0	41.0
48	33.50080943505315	35.0	33.0	39.0	23.0	41.0
49	33.44260571779746	35.0	33.0	39.0	23.0	41.0
50	33.37481896875872	35.0	33.0	39.0	23.0	41.0
51	32.92566418553125	35.0	32.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	0.0
11	8.0
12	20.0
13	22.0
14	41.0
15	121.0
16	266.0
17	580.0
18	1174.0
19	2200.0
20	3862.0
21	6098.0
22	9573.0
23	14274.0
24	21165.0
25	31528.0
26	42002.0
27	50540.0
28	55758.0
29	61231.0
30	68318.0
31	80680.0
32	98319.0
33	130788.0
34	231819.0
35	249852.0
36	248204.0
37	286253.0
38	351811.0
39	426594.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.05582352536863	18.006938014741262	24.167054133496137	26.77018432639397
2	29.961792401337465	21.40992103762943	27.41118249485511	21.217104066177985
3	27.8335280775311	22.078008191385702	30.47943460840244	19.609029122680756
4	24.462041053963684	25.316718755685653	27.119066198202425	23.102173992148238
5	22.26439658274472	34.65449414352311	26.021315392608347	17.05979388112383
6	84.69500632750179	2.3085880169649826	11.558425280896605	1.4379803746366235
7	81.05343807740981	6.476086894995816	11.019273610880877	1.4512014167134997
8	79.70250633760962	3.7665818956629322	13.454735114198266	3.076176652529181
9	46.61128923354344	19.02883966150898	19.250565027715673	15.10930607723191
10	30.954745222028603	18.765025289791495	27.265751032009476	23.014478456170426
11	26.645211112144356	15.966288364270032	36.57926762704532	20.80923289654029
12	22.614450962871917	17.995940695337865	36.90029231845326	22.489316023336958
13	19.194082471809264	21.6489105780466	39.05972919100969	20.097277759134446
14	19.63163023130759	18.99160241455851	39.313071850501146	22.063695503632754
15	24.1021214314305	15.381732320393962	38.06576558728516	22.450380660890378
16	23.985072756162744	16.298310375081368	34.125409872520045	25.591206996235844
17	24.576097811452577	19.826064455612475	33.10803653374196	22.48980119919299
18	25.501004718335203	23.490435970937966	29.78898893394735	21.219570376779483
19	27.234133738724715	21.08780470054542	28.85308470766133	22.824976853068534
20	26.60384987041762	22.84337310427642	31.507360522049222	19.045416503256742
21	23.987822086013594	18.53266648607343	33.01079920592885	24.468712221984127
22	25.61255473390126	20.968330145997502	31.136524442755313	22.282590677345926
23	26.22872807106209	20.12509450821362	32.426607043944806	21.219570376779483
24	27.94156056814093	18.17715387756587	30.351792926944643	23.529492627348557
25	22.20649893059155	20.463949412330745	31.517751371632574	25.81180028544513
26	20.627211087885563	20.155903175071664	33.255934307189094	25.96095142985368
27	22.417388702680192	22.213857431074704	32.87127071599827	22.497483150246833
28	23.9516764847392	22.968871925703404	30.558558704257017	22.520892885300384
29	23.654425410276833	20.20935338187787	34.47550468396858	21.660716523876715
30	29.695875600910515	20.247560980540406	29.879959406953375	20.176604011595703
31	29.280524636825657	20.92769666805481	28.403650139690217	21.38812855542932
32	29.453206810251764	19.58068676642421	27.458608434782256	23.507497988541765
33	24.557661128923357	21.98331803681676	30.994812661472587	22.464208172787295
34	24.477364524750033	23.513441392778155	29.457735118241402	22.55145896423041
35	27.788164134992094	25.732433601662535	25.861611673331097	20.617790590014273
36	25.788107531142224	24.91535702878306	26.352569208314296	22.94396623176042
37	25.357716115520372	28.507073459667737	25.18992613197592	20.945284292835975
38	25.23621999490565	23.687053486594994	25.900223585206987	25.17650293329236
39	23.745557608568205	21.94470612494087	30.818532100447577	23.49120416604335
40	22.335879158866792	22.42438332127132	30.891146753567057	24.348590766294834
41	22.21410001900272	26.14196245547501	26.351881875851586	25.292055649670687
42	21.29748153299398	21.103451621902455	28.452774195113474	29.146292649990098
43	22.658561534449507	20.371361686471275	31.298977491883413	25.671099287195805
44	25.994024250706538	22.782402671701714	28.814674952392117	22.40889812519963
45	26.424860410863086	23.019491940016092	28.565698875604955	21.989948773515867
46	23.734479426522135	23.724978066008177	28.33079289864272	24.209749608826968
47	22.53734843308414	25.821544233887106	29.817048270954544	21.82405906207421
48	24.748213946379984	24.199399190564947	29.14681825716746	21.90556860588761
49	22.90005781678951	21.90698370213437	31.34179426117825	23.85116421989787
50	22.230919448678502	22.87167502921163	32.73796864955344	22.15943687255643
51	21.76737434956112	20.478666413297052	31.32727941681862	26.42667982032321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1022.0
1	8240.5
2	15459.0
3	56146.5
4	96834.0
5	59066.5
6	21299.0
7	19261.5
8	17224.0
9	17214.0
10	17204.0
11	17398.5
12	17593.0
13	16992.0
14	16391.0
15	15097.0
16	13803.0
17	12900.0
18	11997.0
19	11284.5
20	10572.0
21	9941.5
22	9311.0
23	9824.5
24	10338.0
25	10734.0
26	12170.5
27	13211.0
28	14917.5
29	16624.0
30	20010.0
31	23396.0
32	25843.5
33	28291.0
34	32158.0
35	36025.0
36	40082.5
37	44140.0
38	47281.0
39	50422.0
40	56073.5
41	61725.0
42	69189.5
43	76654.0
44	83339.5
45	90025.0
46	97833.5
47	105642.0
48	123145.0
49	140648.0
50	142740.5
51	144833.0
52	136124.0
53	127415.0
54	131226.0
55	135037.0
56	143307.5
57	151578.0
58	158111.0
59	164644.0
60	165322.0
61	166000.0
62	165891.5
63	165783.0
64	151342.0
65	136901.0
66	118777.5
67	100654.0
68	85084.0
69	69514.0
70	60765.0
71	52016.0
72	47683.5
73	43351.0
74	36347.0
75	21898.5
76	14454.0
77	10925.5
78	7397.0
79	5675.0
80	3953.0
81	2855.5
82	1758.0
83	1373.0
84	988.0
85	694.5
86	401.0
87	255.5
88	110.0
89	108.5
90	107.0
91	94.5
92	82.0
93	53.0
94	24.0
95	14.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2473330.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.55375180714356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16576157856346	18.947089578092616
2	8.498727489399524	4.173512909025284
3	2.781401274395982	2.0488150970277523
4	1.4567582698205719	1.4307552400071277
5	0.907121188165732	1.1136614256611277
6	0.6657285128025868	0.9807679604576107
7	0.517449010845271	0.8893720209602702
8	0.42425610086221616	0.8333663202589856
9	0.36451315309363763	0.8055148942350451
>10	5.766178261600318	34.70306811018587
>50	1.257330173737049	20.672767170280093
>100	0.18697023581311473	6.71266217209049
>500	0.00450941163142669	0.7674805528173945
>1k	0.0024281447246143717	1.2066249019824689
>5k	5.203167267030796E-4	0.9974436526517041
>10k+	3.4687781780205307E-4	3.7170979942662457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63400	2.563345772703198	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	23885	0.9657021101106605	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9768	0.3949331468101709	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7344	0.2969276238916764	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6310	0.25512163763023943	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4892	0.19779002397577355	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3901	0.1577225845317851	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3346	0.1352832011902981	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03295152688885025	0.0
2	0.0	0.0	0.0	0.10119959730403949	0.0
3	0.0	0.0	0.0	0.1658088487989876	0.0
4	0.0	0.0	0.0	0.2072913844897365	0.0
5	0.0	0.0	0.0	0.45295209292734895	0.0
6	0.0	0.0	0.0	0.5350276752394545	0.0
7	0.0	0.0	0.0	0.777534740612858	0.0
8	0.0	0.0	0.0	1.0326968095644333	0.0
9	0.0	0.0	0.0	1.265904671030554	0.0
10	0.0	0.0	0.0	1.4125490735162716	0.0
11	0.0	0.0	0.0	1.567926641410568	0.0
12	0.0	0.0	0.0	1.6948809904056474	0.0
13	0.0	0.0	0.0	1.760945769468692	0.0
14	0.0	0.0	0.0	1.805905398794338	0.0
15	0.0	0.0	0.0	1.9493961582158466	0.0
16	0.0	0.0	0.0	2.110878855631881	0.0
17	0.0	0.0	0.0	2.328844108954325	0.0
18	0.0	0.0	0.0	2.443426473620584	0.0
19	0.0	0.0	0.0	2.5788714000962267	0.0
20	0.0	0.0	0.0	2.7365939846280116	0.0
21	0.0	0.0	0.0	2.90381792967376	0.0
22	0.0	0.0	0.0	3.07253783360894	0.0
23	4.043132133601258E-5	0.0	0.0	3.223872269369635	0.0
24	8.086264267202516E-5	0.0	0.0	3.354384574642284	0.0
25	8.086264267202516E-5	0.0	0.0	3.4845329980229085	0.0
26	8.086264267202516E-5	0.0	0.0	3.621433452066647	0.0
27	8.086264267202516E-5	0.0	0.0	3.774870316536815	0.0
28	8.086264267202516E-5	0.0	0.0	3.9262451836188457	0.0
29	8.086264267202516E-5	0.0	0.0	4.087404430464192	0.0
30	8.086264267202516E-5	0.0	0.0	4.249857479592291	0.0
31	8.086264267202516E-5	0.0	0.0	4.418334795599455	0.0
32	8.086264267202516E-5	0.0	0.0	4.604197579781105	0.0
33	8.086264267202516E-5	0.0	0.0	4.7848447235104095	0.0
34	8.086264267202516E-5	0.0	0.0	5.001475743228765	0.0
35	8.086264267202516E-5	0.0	0.0	5.189157936870535	0.0
36	8.086264267202516E-5	0.0	0.0	5.392082738655982	0.0
37	8.086264267202516E-5	0.0	0.0	5.636611370096186	0.0
38	8.086264267202516E-5	0.0	0.0	5.904266717340589	0.0
39	8.086264267202516E-5	0.0	0.0	6.2376229617560135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	95	0.0	45.0	8
CGTTTTT	18715	0.0	43.593376	1
AATACGG	190	0.0	41.44737	1
CTTACTG	7400	0.0	41.0777	38
GCACTTA	7450	0.0	40.892616	35
CGCACTT	7460	0.0	40.86796	34
CACTTAC	7475	0.0	40.785954	36
CCGCACT	7490	0.0	40.674236	33
ACCCGCA	7560	0.0	40.59524	31
ACTTACT	7505	0.0	40.532978	37
AATGCGG	400	0.0	40.5	1
CCCGCAC	7625	0.0	40.485245	32
CGTTTTA	960	0.0	40.312496	1
CACGACG	415	0.0	40.120483	25
CGACGGT	410	0.0	40.060978	27
CACGACC	710	0.0	39.929577	26
CGCATCG	85	0.0	39.705883	20
CTTATGA	7950	0.0	39.396225	25
TTATCGG	80	0.0	39.375	1
ACGACCA	715	0.0	39.335663	27
>>END_MODULE