##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043470_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1053654
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51145157708318	31.0	31.0	33.0	30.0	34.0
2	30.555395794065223	31.0	31.0	34.0	26.0	34.0
3	31.455341127163187	31.0	31.0	34.0	28.0	34.0
4	35.07611511938454	37.0	35.0	37.0	32.0	37.0
5	35.396466012562	37.0	35.0	37.0	33.0	37.0
6	35.81835213457169	37.0	35.0	37.0	35.0	37.0
7	33.799951407198186	37.0	35.0	37.0	32.0	37.0
8	34.80356075144213	37.0	35.0	37.0	32.0	37.0
9	36.80052654856338	39.0	37.0	39.0	32.0	39.0
10	36.73548527315418	39.0	35.0	39.0	32.0	39.0
11	36.4562152281489	39.0	35.0	39.0	32.0	39.0
12	36.397665647356725	39.0	35.0	39.0	32.0	39.0
13	36.443109407832175	39.0	35.0	39.0	32.0	39.0
14	37.79348343953518	40.0	36.0	41.0	33.0	41.0
15	37.94144092842622	40.0	36.0	41.0	33.0	41.0
16	37.90908021039164	40.0	36.0	41.0	33.0	41.0
17	37.854760670960296	40.0	36.0	41.0	33.0	41.0
18	37.53338002797882	39.0	36.0	41.0	33.0	41.0
19	37.17519793025035	38.0	35.0	41.0	33.0	41.0
20	36.819968414678826	38.0	35.0	40.0	32.0	41.0
21	36.71687385042908	38.0	35.0	40.0	32.0	41.0
22	36.616261125568734	38.0	35.0	40.0	32.0	41.0
23	36.53317787433066	37.0	35.0	40.0	32.0	41.0
24	36.35040155496966	37.0	35.0	40.0	32.0	41.0
25	36.24267928560989	36.0	35.0	40.0	32.0	41.0
26	35.96427954527767	36.0	35.0	40.0	31.0	41.0
27	35.84064028609012	36.0	35.0	40.0	31.0	41.0
28	35.7213003509691	36.0	35.0	40.0	31.0	41.0
29	35.63544389334639	36.0	35.0	40.0	30.0	41.0
30	35.43542092565491	35.0	35.0	40.0	30.0	41.0
31	34.89883111533767	35.0	34.0	40.0	27.0	41.0
32	34.666915325144686	35.0	34.0	40.0	24.0	41.0
33	34.42475708344485	35.0	34.0	40.0	23.0	41.0
34	34.134948474546675	35.0	34.0	40.0	21.0	41.0
35	33.861349171549676	35.0	33.0	40.0	20.0	41.0
36	33.66102060069055	35.0	33.0	40.0	18.0	41.0
37	33.54740645411112	35.0	33.0	40.0	18.0	41.0
38	33.45416427024431	35.0	33.0	40.0	18.0	41.0
39	33.32428387307408	35.0	33.0	40.0	18.0	41.0
40	33.2666710324262	35.0	33.0	40.0	18.0	41.0
41	33.17649152378295	35.0	33.0	40.0	17.0	41.0
42	33.084136727996096	35.0	33.0	40.0	16.0	41.0
43	32.92289594117234	35.0	33.0	40.0	15.0	41.0
44	32.742322432221584	35.0	33.0	40.0	15.0	41.0
45	32.578965201100175	35.0	32.0	40.0	15.0	41.0
46	32.58762838654815	35.0	32.0	40.0	15.0	41.0
47	32.54175943905685	35.0	32.0	40.0	15.0	41.0
48	32.46566330123551	35.0	32.0	39.0	13.0	41.0
49	32.36352445869327	35.0	32.0	39.0	12.0	41.0
50	32.2551577652626	35.0	32.0	39.0	12.0	41.0
51	31.846135448638737	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	3.0
12	5.0
13	15.0
14	35.0
15	70.0
16	151.0
17	298.0
18	748.0
19	1395.0
20	2212.0
21	3693.0
22	5762.0
23	8941.0
24	14474.0
25	21733.0
26	27806.0
27	29305.0
28	28763.0
29	27968.0
30	29390.0
31	33753.0
32	40794.0
33	52823.0
34	87806.0
35	123056.0
36	118918.0
37	94974.0
38	119774.0
39	178917.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.361033128522266	17.868958880239624	24.909695213039576	32.860312778198534
2	34.19120508250336	19.20326786592183	27.846902303792326	18.75862474778248
3	20.805976155360298	20.64339906648672	40.56986449062026	17.980760287532718
4	21.26979065233938	23.704081225905277	38.06116618928036	16.964961932474985
5	19.22993696222859	29.868913324487924	35.96930301598058	14.931846697302909
6	75.51207512143455	1.932323134539422	21.276434199462063	1.279167544563965
7	72.153951866552	6.912041334252041	19.508016863220753	1.4259899359751873
8	71.02938915431442	3.8510744513853696	22.559682780115672	2.5598536141845427
9	39.498070524099944	21.096963519333674	28.387876855210536	11.017089101355854
10	28.836790825071606	24.459357625937926	36.28828818568524	10.415563363305221
11	26.36282878440171	22.00978689398987	39.111226265927904	12.516158055680519
12	20.1219755251724	27.131487186495757	38.09087233570033	14.655664952631508
13	14.612386988518052	30.176699371900074	38.164520800946036	17.046392838635832
14	14.069039741698889	24.606369832981226	45.031291106947826	16.293299318372064
15	16.411269733707652	22.195141858712635	44.967418146754056	16.42617026082566
16	17.51011242779888	23.369815897818448	38.01504099068575	21.105030683696928
17	18.595762935460787	23.488071036602147	41.85871263242013	16.057453395516934
18	21.86932332625321	25.563894788991455	37.038344655835786	15.52843722891955
19	21.769195580332823	25.797178200813548	37.341764943710174	15.091861275143453
20	17.660351500587478	28.106854811921185	38.39400790012661	15.838785787364731
21	18.306483912176102	25.32605580199952	37.70383826189622	18.66362202392816
22	15.445867428966245	28.61451671990995	37.82323229447238	18.116383556651424
23	14.787871540372835	25.923405596144466	44.212995917065754	15.075726946416946
24	15.790667524633323	26.354951435670532	42.34416611145594	15.5102149282402
25	14.955668559128519	30.552534323411667	38.029941517803756	16.461855599656054
26	15.256716151601948	30.503561890335916	38.30545890776289	15.934263050299244
27	15.142352233275819	28.86469372298686	41.04089198161825	14.952062062119065
28	14.485685054106945	26.872293940895208	41.25509892241666	17.386922082581187
29	15.675259620330772	30.259743710933567	39.084367353989066	14.980629314746588
30	20.3418769349331	28.6275190907072	35.94699967921159	15.083604295148124
31	19.414532664423046	28.249121628162566	36.591044118847364	15.745301588567026
32	18.698453192414206	30.487047930345256	35.30589738187299	15.50860149536755
33	16.437464290934216	29.999221755908483	35.86329098546582	17.70002296769148
34	16.56378659408117	29.780838871204402	35.97338405207022	17.68199048264421
35	20.50037298771703	30.098495331484532	33.408690139267726	15.992441541530711
36	17.93966520318814	32.02218185476447	34.29446478635301	15.743688155694374
37	16.84395446702618	32.76920127480179	33.478637199687945	16.908207058484095
38	17.519603209402707	32.458567992908485	33.22741621063461	16.794412587054193
39	19.426016510163677	30.070307710121156	32.731902503098745	17.771773276616422
40	16.356982462933754	29.366281530749184	34.6335704130578	19.64316559325927
41	17.162939636730844	29.71943351422763	32.83639600855689	20.28123084048464
42	17.324282923995924	28.745964045122975	34.30424029140496	19.625512739476147
43	16.68859037217151	30.660539418063237	34.385671197565806	18.265199012199453
44	15.5191362629478	35.11228543715489	32.08055016162801	17.288028138269297
45	17.861840794036752	32.471760179337814	33.17834886974282	16.48805015688262
46	17.004728307395027	31.69161793150313	32.29153023668111	19.01212352442073
47	16.533700816397033	30.944123972385622	34.60955873560011	17.91261647561723
48	17.897715948499222	29.208354924861478	34.70513090635066	18.188798220288636
49	17.3123245391751	32.07722838806667	32.86534289244856	17.745104180309664
50	16.102724423767196	31.597943917073344	33.40916467834792	18.890166980811536
51	16.287130310329577	29.20484333566807	35.26290414120765	19.245122212794712
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	579.0
1	6139.0
2	11699.0
3	49032.5
4	86366.0
5	51832.0
6	17298.0
7	17249.5
8	17201.0
9	19364.0
10	21527.0
11	23518.0
12	25509.0
13	24982.5
14	24456.0
15	21979.0
16	19502.0
17	17022.5
18	14543.0
19	13001.5
20	11460.0
21	10365.0
22	9270.0
23	8867.0
24	8464.0
25	8376.0
26	8801.0
27	9314.0
28	10021.5
29	10729.0
30	12810.5
31	14892.0
32	17381.0
33	19870.0
34	21875.0
35	23880.0
36	26700.0
37	29520.0
38	33139.5
39	36759.0
40	41949.0
41	47139.0
42	54244.5
43	61350.0
44	64707.0
45	68064.0
46	67935.0
47	67806.0
48	68947.5
49	70089.0
50	70476.5
51	70864.0
52	65624.5
53	60385.0
54	54631.5
55	48878.0
56	43353.0
57	37828.0
58	34624.5
59	31421.0
60	25702.5
61	19984.0
62	17415.0
63	14846.0
64	12548.5
65	10251.0
66	8548.5
67	6846.0
68	5923.0
69	5000.0
70	4358.5
71	3717.0
72	3495.5
73	3274.0
74	2730.5
75	1727.5
76	1268.0
77	959.5
78	651.0
79	503.5
80	356.0
81	274.5
82	193.0
83	120.5
84	48.0
85	42.5
86	37.0
87	29.5
88	22.0
89	17.5
90	13.0
91	8.0
92	3.0
93	3.5
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1053654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.80490948120208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.15604404723462	32.30408987152624
2	8.19777951174854	6.526237428240073
3	3.085128986493083	3.6840984013556968
4	1.5989266444687937	2.545805214006501
5	1.004349650538822	1.9989023463587374
6	0.6998057112617114	1.6713421794720396
7	0.5081783334877039	1.4159594795350328
8	0.39160159206532397	1.2470132739883877
9	0.30946412802142237	1.1086372443214667
>10	2.528233676340342	21.21919100822323
>50	0.4034701901658213	11.063881325756594
>100	0.11336073041937965	6.813593555129967
>500	0.002194078652644615	0.5417687504187821
>1k	9.751460678420509E-4	0.6259015655026232
>5k	2.4378651696051271E-4	0.6654934784832542
>10k+	2.4378651696051271E-4	6.568084877681403
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67685	6.423835528551118	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6858	0.650877802390538	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2842	0.2697280131807975	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	1251	0.11872967786389081	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1210	0.11483845740632125	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1147	0.10885926499590948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06833362754756306	0.0
2	0.0	0.0	0.0	0.20946154999648842	0.0
3	0.0	0.0	0.0	0.35656866485582556	0.0
4	0.0	0.0	0.0	0.45365936066298806	0.0
5	0.0	0.0	0.0	0.9980505934585737	0.0
6	0.0	0.0	0.0	1.2043801855257987	0.0
7	0.0	0.0	0.0	1.7171671155806365	0.0
8	0.0	0.0	0.0	2.2983825809990757	0.0
9	0.0	0.0	0.0	2.7927573947424866	0.0
10	0.0	0.0	0.0	3.0907679371026924	0.0
11	0.0	0.0	0.0	3.416586469562114	0.0
12	0.0	0.0	0.0	3.689446440672175	0.0
13	0.0	0.0	0.0	3.814345126578554	0.0
14	0.0	0.0	0.0	3.91456778031498	0.0
15	0.0	0.0	0.0	4.2109648898025345	0.0
16	0.0	0.0	0.0	4.576170165917844	0.0
17	0.0	0.0	0.0	5.012556304061865	0.0
18	0.0	0.0	0.0	5.248497134733034	0.0
19	0.0	0.0	0.0	5.533125675031841	0.0
20	0.0	0.0	0.0	5.855337710481809	0.0
21	0.0	0.0	0.0	6.193209535578093	0.0
22	0.0	0.0	0.0	6.526241062056425	0.0
23	0.0	0.0	0.0	6.807642736609931	0.0
24	3.796312641531281E-4	0.0	0.0	7.067025797842556	0.0
25	3.796312641531281E-4	0.0	0.0	7.313786119542089	0.0
26	3.796312641531281E-4	0.0	0.0	7.5851275655955375	0.0
27	3.796312641531281E-4	0.0	0.0	7.859316246130134	0.0
28	3.796312641531281E-4	0.0	0.0	8.143185523900636	0.0
29	4.745390801914101E-4	0.0	0.0	8.481342072445035	0.0
30	4.745390801914101E-4	0.0	0.0	8.797954546748743	0.0
31	4.745390801914101E-4	0.0	0.0	9.09036552796269	0.0
32	4.745390801914101E-4	0.0	0.0	9.393880723653115	0.0
33	4.745390801914101E-4	0.0	0.0	9.69967370692846	0.0
34	4.745390801914101E-4	0.0	0.0	10.043619632251195	0.0
35	4.745390801914101E-4	0.0	0.0	10.356815425177524	0.0
36	4.745390801914101E-4	0.0	0.0	10.668967232127434	0.0
37	4.745390801914101E-4	0.0	0.0	11.009401568256752	0.0
38	4.745390801914101E-4	0.0	0.0	11.349646088753993	0.0
39	4.745390801914101E-4	0.0	0.0	11.713048116364575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGG	70	0.0	45.000004	1
CACGACG	225	0.0	45.000004	25
TACCCGA	25	3.8905833E-5	45.0	39
CGAACGT	20	7.0330204E-4	45.0	42
CTACTCG	25	3.8905833E-5	45.0	42
ACGTTCT	25	3.8905833E-5	45.0	43
GACGTTG	20	7.0330204E-4	45.0	27
CACATCG	25	3.8905833E-5	45.0	16
TTGTCGT	25	3.8905833E-5	45.0	16
GCACGGT	20	7.0330204E-4	45.0	21
TGACGTT	20	7.0330204E-4	45.0	41
CGTTATG	25	3.8905833E-5	45.0	42
AATTCGC	25	3.8905833E-5	45.0	12
GTATCGG	75	0.0	45.0	1
ACATCGC	25	3.8905833E-5	45.0	17
ATACGCC	20	7.0330204E-4	45.0	10
AATACGG	25	3.8905833E-5	45.0	1
AATACGC	20	7.0330204E-4	45.0	9
GCGTAAC	20	7.0330204E-4	45.0	15
CCGCAAT	20	7.0330204E-4	45.0	32
>>END_MODULE