FastQCFastQC Report
Thu 26 May 2016
SRR1043469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043469_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1732090
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT515942.9787135772390583No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC146810.8475887511618911No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57460.33173795818923957No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC48480.27989307714957073No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC31310.1807642789924311No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA22200.12816885958581828No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21820.1259749782055205No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC19660.11350449457014358No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC18080.10438256672574751No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT207.034279E-445.018
ACCGCAT253.8916274E-545.018
CGAATCG253.8916274E-545.035
ATAAGCG551.8189894E-1245.032
CTCGAAT253.8916274E-545.025
CCGAATA207.034279E-445.08
AATACGA207.034279E-445.023
ATCGCAC253.8916274E-545.038
CGTTTTT140150.043.7959331
CGGTCTA5450.043.76146730
TCACGAC5450.042.93577624
ATTAGCG1750.042.4285710
CACGACG5750.041.86956425
GCGTAGG2650.041.6037751
CACTTAC38900.041.41388336
GCGCGAC24900.041.295188
CGCACTT38950.041.1874234
ACGGGCT7900.041.012664
CGACGGT5800.040.73275827
ACCCGCA39500.040.67088731