##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043469_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1732090
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.498666928392865	31.0	31.0	33.0	30.0	34.0
2	30.53845123521295	31.0	31.0	34.0	26.0	34.0
3	31.526940863350056	31.0	31.0	34.0	28.0	34.0
4	35.15753973523316	37.0	35.0	37.0	32.0	37.0
5	35.47069667280569	37.0	35.0	37.0	33.0	37.0
6	35.739176948080065	37.0	35.0	37.0	35.0	37.0
7	33.686430843662855	37.0	35.0	37.0	32.0	37.0
8	34.70584265251806	37.0	35.0	37.0	31.0	37.0
9	36.64350755445733	39.0	37.0	39.0	32.0	39.0
10	36.63580241211484	39.0	35.0	39.0	32.0	39.0
11	36.471006125547746	39.0	35.0	39.0	32.0	39.0
12	36.45714137256147	39.0	35.0	39.0	32.0	39.0
13	36.50438891743501	39.0	35.0	39.0	32.0	39.0
14	37.75379743546814	40.0	36.0	41.0	33.0	41.0
15	37.86267630434908	40.0	36.0	41.0	33.0	41.0
16	37.700584265251806	40.0	36.0	41.0	33.0	41.0
17	37.69443793336374	40.0	36.0	41.0	33.0	41.0
18	37.54280493507843	39.0	36.0	41.0	33.0	41.0
19	37.366714778100445	39.0	35.0	41.0	33.0	41.0
20	37.109295706343204	39.0	35.0	41.0	32.0	41.0
21	37.02195613391914	38.0	35.0	41.0	32.0	41.0
22	36.96474952225346	38.0	35.0	41.0	32.0	41.0
23	36.89482705864014	38.0	35.0	40.0	32.0	41.0
24	36.779583047070304	38.0	35.0	40.0	32.0	41.0
25	36.66057421958443	38.0	35.0	40.0	31.0	41.0
26	36.398940008890996	38.0	35.0	40.0	31.0	41.0
27	36.29398414632034	38.0	35.0	40.0	31.0	41.0
28	36.10097974123747	38.0	35.0	40.0	30.0	41.0
29	36.02092212298437	38.0	35.0	40.0	30.0	41.0
30	35.896075261678085	38.0	35.0	40.0	30.0	41.0
31	35.70325791384974	38.0	35.0	40.0	29.0	41.0
32	35.62007228261811	38.0	35.0	40.0	29.0	41.0
33	35.40123088292179	38.0	34.0	40.0	27.0	41.0
34	35.325933987263944	38.0	34.0	40.0	27.0	41.0
35	35.190537443204455	38.0	34.0	40.0	26.0	41.0
36	35.05879197963154	37.0	34.0	40.0	26.0	41.0
37	34.952027896933764	37.0	34.0	40.0	25.0	41.0
38	34.8573388218857	37.0	34.0	40.0	25.0	41.0
39	34.72346009733905	37.0	34.0	40.0	24.0	41.0
40	34.621080313378634	37.0	34.0	40.0	24.0	41.0
41	34.50792914917816	36.0	34.0	40.0	23.0	41.0
42	34.38722987835505	36.0	34.0	40.0	23.0	41.0
43	34.21538257249912	36.0	33.0	40.0	23.0	41.0
44	33.99214359530971	35.0	33.0	40.0	22.0	41.0
45	33.7799179026494	35.0	33.0	40.0	22.0	41.0
46	33.756857322656444	35.0	33.0	40.0	22.0	41.0
47	33.73870988228094	35.0	33.0	40.0	23.0	41.0
48	33.63657777598162	35.0	33.0	40.0	22.0	41.0
49	33.580222159356616	35.0	33.0	39.0	22.0	41.0
50	33.49638817844338	35.0	33.0	39.0	22.0	41.0
51	33.060773400920276	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	7.0
12	10.0
13	18.0
14	57.0
15	98.0
16	186.0
17	423.0
18	940.0
19	1661.0
20	2941.0
21	4734.0
22	7001.0
23	10580.0
24	16113.0
25	23034.0
26	30505.0
27	35018.0
28	38290.0
29	40771.0
30	45938.0
31	53933.0
32	65677.0
33	87705.0
34	153476.0
35	182083.0
36	170215.0
37	191018.0
38	244824.0
39	324658.0
40	170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.01859603138405	17.30002482549983	23.54629378381031	28.135085359305812
2	30.920448706475987	20.929397433158787	27.90709489691644	20.24305896344878
3	26.643072819541707	21.786569981929347	31.747484253127723	19.822872945401222
4	24.17212731440052	25.29435537414337	28.729049876161167	21.80446743529493
5	21.851231748927596	34.17282011904693	27.67968177173242	16.296266360293057
6	83.8671200688186	1.9748973783117505	12.722837727831696	1.43514482503796
7	79.26337545970476	7.4726486498969455	11.91802966358561	1.3459462268126945
8	78.91160390049016	3.5917302218706877	14.742536473277948	2.7541294043612052
9	42.48711094689075	19.44748829448816	22.705633079112516	15.35976767950857
10	29.529932047410934	20.105479507415897	29.724494685611024	20.64009375956215
11	26.870024074961464	17.13652292894711	36.97671599050857	19.01673700558285
12	21.22343527183922	21.67139120946371	36.818525596245	20.286647922452065
13	17.284783123278814	25.039576465426162	38.93775727589213	18.737883135402893
14	17.49262451720176	20.534787453307853	41.18885277323926	20.783735256251116
15	21.70891812781091	17.062046429458054	40.32359750359392	20.905437939137112
16	20.4628512375223	18.15194360570178	36.4143318187854	24.970873337990522
17	21.3320901338845	20.509500083713895	35.77620100572141	22.382208776680194
18	23.828669410942847	24.099209625365887	30.98054950955205	21.091571454139217
19	25.616163132400743	21.988580270078344	31.43404788434781	20.9612087131731
20	23.87041089088904	24.129231160043645	34.0479998152521	17.95235813381522
21	22.373260049997402	19.984642830337915	33.71591545473965	23.926181664925032
22	22.007516930413544	21.98713692706499	32.28844921453273	23.71689692798873
23	22.62307385874868	21.08949304019999	37.0185152041753	19.26891789687603
24	25.069828934986056	19.394604206478878	33.403402825488286	22.13216403304678
25	19.910801401774737	22.98633442834957	32.30345998187161	24.799404188004086
26	18.632750030310202	22.83749689681252	33.96261164258208	24.56714143029519
27	20.182034420844182	23.363104688555445	34.98455622975711	21.470304660843258
28	21.942508761092093	23.018145708363885	33.42262815442616	21.61671737611787
29	21.30836157474496	22.71885410111484	35.27149282081185	20.70129150332835
30	25.86580373999042	22.99193459924138	31.13337066780594	20.008890992962257
31	26.84254282398721	22.194805119826334	30.37440317766398	20.588248878522478
32	26.934570374518646	21.89187628818364	28.68095768695622	22.492595650341496
33	20.979048432818157	23.75124849170655	32.523714125709404	22.745988949765888
34	21.746387312437577	23.50888233290418	32.60656201467591	22.138168339982332
35	25.542783573601834	25.652362175175654	28.08433741895629	20.720516832266224
36	22.63127204706453	25.962680923046722	29.744528286636374	21.661518743252373
37	23.26807498455623	28.462781957057658	28.347834119474165	19.92130893891195
38	22.429088557753925	25.9070256164518	27.81194972547616	23.851936100318113
39	22.651998452736287	23.103649348474963	31.89020201028815	22.354150188500597
40	20.386180856652945	23.506457516641746	31.98534718172843	24.122014444976877
41	21.035050141736285	26.860728945955465	27.478075619627155	24.626145292681095
42	20.319152007112795	23.01970451881831	29.88990179494137	26.771241679127527
43	21.345946226812696	21.681032740792915	32.826007886426225	24.147013145968167
44	23.064679087114413	25.041597145644857	30.596562534279396	21.297161232961336
45	23.717012395429798	24.941775542841306	30.414181711112008	20.927030350616885
46	20.849436230218984	26.294707549838638	29.903122816943693	22.95273340299869
47	20.507825805818406	27.110600488427277	31.713305890571508	20.668267815182816
48	23.315820771437973	25.703629719009978	30.27694865740233	20.703600852149716
49	21.456621769076666	24.224838201248204	31.146014352602926	23.17252567707221
50	20.17822399528893	25.1689577331432	32.64224145396602	22.01057681760186
51	20.013798359207662	22.49207604685669	32.992800605049396	24.501324988886257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	863.0
1	6990.5
2	13118.0
3	43950.0
4	74782.0
5	45473.0
6	16164.0
7	14891.0
8	13618.0
9	13992.5
10	14367.0
11	14599.0
12	14831.0
13	14337.0
14	13843.0
15	12894.5
16	11946.0
17	11006.0
18	10066.0
19	9561.5
20	9057.0
21	8983.0
22	8909.0
23	8920.0
24	8931.0
25	9857.0
26	11439.5
27	12096.0
28	14870.5
29	17645.0
30	20795.0
31	23945.0
32	26399.5
33	28854.0
34	32479.0
35	36104.0
36	39692.5
37	43281.0
38	45882.0
39	48483.0
40	55755.0
41	63027.0
42	68830.0
43	74633.0
44	79802.5
45	84972.0
46	87880.5
47	90789.0
48	99117.0
49	107445.0
50	103710.0
51	99975.0
52	90776.0
53	81577.0
54	83991.0
55	86405.0
56	89068.5
57	91732.0
58	93041.0
59	94350.0
60	92936.0
61	91522.0
62	88313.0
63	85104.0
64	79795.5
65	74487.0
66	63076.5
67	51666.0
68	42788.0
69	33910.0
70	29639.0
71	25368.0
72	25522.0
73	25676.0
74	22006.5
75	14094.5
76	9852.0
77	7195.5
78	4539.0
79	3456.0
80	2373.0
81	1846.0
82	1319.0
83	1041.5
84	764.0
85	584.5
86	405.0
87	255.0
88	105.0
89	70.5
90	36.0
91	23.5
92	11.0
93	11.0
94	11.0
95	10.5
96	10.0
97	6.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1732090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.498533594605377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2439194897302	20.20603743277089
2	8.629518930792411	4.400801567241866
3	2.7702133530648467	2.119091347420452
4	1.3798794399691425	1.4073960902623373
5	0.8218044636573475	1.0477404362381764
6	0.5640976698355595	0.8630198030964373
7	0.4023760571281421	0.7181999587242748
8	0.3332400260292405	0.6797705599019007
9	0.2733947165543717	0.6274047928184334
>10	3.8233988352024477	24.972417074674496
>50	1.457442965631372	25.722175543141407
>100	0.2921017168110855	10.601405131523906
>500	0.005502325518259128	0.9741785548914276
>1k	0.0023923154427213603	1.2670421267157073
>5k	2.3923154427213603E-4	0.35050907804672154
>10k+	4.7846308854427206E-4	4.042810502531582
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51594	2.9787135772390583	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	14681	0.8475887511618911	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5746	0.33173795818923957	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4848	0.27989307714957073	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3131	0.1807642789924311	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2220	0.12816885958581828	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2182	0.1259749782055205	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	1966	0.11350449457014358	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	1808	0.10438256672574751	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037642385788267355	0.0
2	0.0	0.0	0.0	0.12430070031003009	0.0
3	0.0	0.0	0.0	0.18832739638240506	0.0
4	0.0	0.0	0.0	0.23076168097500707	0.0
5	0.0	0.0	0.0	0.492526369876854	0.0
6	0.0	0.0	0.0	0.5551097229358751	0.0
7	0.0	0.0	0.0	0.7498455622975712	0.0
8	0.0	0.0	0.0	0.9606891096882957	0.0
9	0.0	0.0	0.0	1.1310035852640452	0.0
10	0.0	0.0	0.0	1.26996865058975	0.0
11	0.0	0.0	0.0	1.4323736064523207	0.0
12	0.0	0.0	0.0	1.5715118729396278	0.0
13	0.0	0.0	0.0	1.6383675213181763	0.0
14	0.0	0.0	0.0	1.6911361418863915	0.0
15	0.0	0.0	0.0	1.8805604789589456	0.0
16	0.0	0.0	0.0	2.086727594986404	0.0
17	0.0	0.0	0.0	2.344797325774065	0.0
18	0.0	0.0	0.0	2.4893048282710484	0.0
19	0.0	0.0	0.0	2.639585702821447	0.0
20	0.0	0.0	0.0	2.827855365483318	0.0
21	0.0	0.0	0.0	3.0130074072363446	0.0
22	0.0	0.0	0.0	3.1983326501509737	0.0
23	5.773372053415238E-5	0.0	0.0	3.3842929639914785	0.0
24	4.0413604373906666E-4	0.0	0.0	3.527992194400984	0.0
25	6.350709258756762E-4	0.0	0.0	3.6786772049951213	0.0
26	6.350709258756762E-4	0.0	0.0	3.828496209781247	0.0
27	6.350709258756762E-4	0.0	0.0	3.9886495505429855	0.0
28	6.350709258756762E-4	0.0	0.0	4.163986859805206	0.0
29	6.350709258756762E-4	0.0	0.0	4.34867703179396	0.0
30	6.928046464098285E-4	0.0	0.0	4.530769186358676	0.0
31	6.928046464098285E-4	0.0	0.0	4.709224116529741	0.0
32	6.928046464098285E-4	0.0	0.0	4.912215877927821	0.0
33	6.928046464098285E-4	0.0	0.0	5.113013757945604	0.0
34	6.928046464098285E-4	0.0	0.0	5.3385794040725365	0.0
35	6.928046464098285E-4	0.0	0.0	5.530832693451264	0.0
36	6.928046464098285E-4	0.0	0.0	5.748662021026621	0.0
37	6.928046464098285E-4	0.0	0.0	5.986813618229999	0.0
38	6.928046464098285E-4	0.0	0.0	6.2367428944223455	0.0
39	6.928046464098285E-4	0.0	0.0	6.547465778337154	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	20	7.034279E-4	45.0	18
ACCGCAT	25	3.8916274E-5	45.0	18
CGAATCG	25	3.8916274E-5	45.0	35
ATAAGCG	55	1.8189894E-12	45.0	32
CTCGAAT	25	3.8916274E-5	45.0	25
CCGAATA	20	7.034279E-4	45.0	8
AATACGA	20	7.034279E-4	45.0	23
ATCGCAC	25	3.8916274E-5	45.0	38
CGTTTTT	14015	0.0	43.795933	1
CGGTCTA	545	0.0	43.761467	30
TCACGAC	545	0.0	42.935776	24
ATTAGCG	175	0.0	42.42857	10
CACGACG	575	0.0	41.869564	25
GCGTAGG	265	0.0	41.603775	1
CACTTAC	3890	0.0	41.413883	36
GCGCGAC	2490	0.0	41.29518	8
CGCACTT	3895	0.0	41.18742	34
ACGGGCT	790	0.0	41.01266	4
CGACGGT	580	0.0	40.732758	27
ACCCGCA	3950	0.0	40.670887	31
>>END_MODULE