FastQCFastQC Report
Thu 26 May 2016
SRR1043468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043468_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4244303
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1062522.5034027966429355No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC367120.8649712332036615No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC157080.37009610294081263No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123100.29003584334106214No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC100930.23780111834616896No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC67080.15804715167602312No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC56000.13194156967586904No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49490.11660336220104928No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA44210.10416315706018162No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA302.1668893E-645.00000415
CGTTTTT333950.043.9961051
ACGGGAT5500.041.7272724
CCTACGG650.041.5384641
CGTTTTA17900.040.9776541
ACGTAGG4300.040.8139531
CGCACTT115450.040.79038634
AACACGT6900.040.7608740
CTTACTG115300.040.74588438
GCACTTA115550.040.69666735
AAACACG7100.040.5633839
CACTTAC116350.040.51353536
CCGCACT116250.040.50967833
TAGTAGG9000.040.51
ACCCGCA116250.040.49032231
TAGCATA42400.040.48938829
ACTTACT116450.040.4014637
TATACGG3400.040.367651
TCACGAC9700.040.36082524
CCCGCAC117650.040.2571232