##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043468_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4244303
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57461307545668	31.0	31.0	33.0	30.0	34.0
2	30.605877101611267	31.0	31.0	34.0	26.0	34.0
3	31.598567067431333	31.0	31.0	34.0	28.0	34.0
4	35.204568099874116	37.0	35.0	37.0	33.0	37.0
5	35.52711952940212	37.0	35.0	37.0	33.0	37.0
6	35.78386510105428	37.0	35.0	37.0	35.0	37.0
7	33.67787526008393	37.0	35.0	37.0	32.0	37.0
8	34.720583332528335	37.0	35.0	37.0	31.0	37.0
9	36.80445599666188	39.0	37.0	39.0	32.0	39.0
10	36.73875451399205	39.0	35.0	39.0	32.0	39.0
11	36.45604354825751	38.0	35.0	39.0	33.0	39.0
12	36.42632418090791	39.0	35.0	39.0	32.0	39.0
13	36.415931190586534	39.0	35.0	39.0	32.0	39.0
14	37.532864877931665	40.0	35.0	41.0	33.0	41.0
15	37.66944702110099	40.0	35.0	41.0	33.0	41.0
16	37.47131743421711	40.0	35.0	41.0	32.0	41.0
17	37.39894394910071	39.0	35.0	41.0	32.0	41.0
18	37.30981058609623	39.0	35.0	41.0	32.0	41.0
19	37.1817756649325	38.0	35.0	41.0	32.0	41.0
20	36.90707685101653	38.0	35.0	41.0	32.0	41.0
21	36.86652625884627	38.0	35.0	41.0	32.0	41.0
22	36.83870378717071	38.0	35.0	40.0	32.0	41.0
23	36.78389714400692	38.0	35.0	40.0	32.0	41.0
24	36.689318363933964	38.0	35.0	40.0	32.0	41.0
25	36.5647904968142	38.0	35.0	40.0	31.0	41.0
26	36.30371370752748	38.0	35.0	40.0	31.0	41.0
27	36.16008117233854	38.0	35.0	40.0	31.0	41.0
28	36.031534977592315	37.0	35.0	40.0	30.0	41.0
29	35.88762512949712	37.0	35.0	40.0	30.0	41.0
30	35.72367854038696	37.0	34.0	40.0	30.0	41.0
31	35.60725282808508	37.0	34.0	40.0	29.0	41.0
32	35.51967684682267	37.0	34.0	40.0	29.0	41.0
33	35.10895381408915	37.0	34.0	40.0	26.0	41.0
34	35.09481910221773	37.0	34.0	40.0	26.0	41.0
35	35.03257401745351	37.0	34.0	40.0	26.0	41.0
36	34.95733881393482	37.0	34.0	40.0	26.0	41.0
37	34.849304585464324	37.0	34.0	40.0	26.0	41.0
38	34.747265216456036	36.0	34.0	40.0	25.0	41.0
39	34.619398049573746	36.0	34.0	40.0	24.0	41.0
40	34.52150753610192	36.0	34.0	40.0	24.0	41.0
41	34.42332863605638	36.0	34.0	40.0	24.0	41.0
42	34.304733427373115	35.0	33.0	40.0	23.0	41.0
43	34.13965049149413	35.0	33.0	40.0	23.0	41.0
44	33.91925458667772	35.0	33.0	40.0	23.0	41.0
45	33.68851139986943	35.0	33.0	40.0	23.0	41.0
46	33.70790186280291	35.0	33.0	39.0	23.0	41.0
47	33.66914803207971	35.0	33.0	39.0	23.0	41.0
48	33.58419651000412	35.0	33.0	39.0	23.0	41.0
49	33.526369582944476	35.0	33.0	39.0	23.0	41.0
50	33.47712474816242	35.0	33.0	39.0	23.0	41.0
51	33.03697544685193	35.0	33.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	5.0
11	13.0
12	19.0
13	40.0
14	79.0
15	197.0
16	469.0
17	984.0
18	1967.0
19	3622.0
20	6529.0
21	10676.0
22	16192.0
23	24389.0
24	37423.0
25	55107.0
26	72776.0
27	86628.0
28	94446.0
29	102679.0
30	116183.0
31	137139.0
32	169098.0
33	229492.0
34	435305.0
35	439147.0
36	416289.0
37	480436.0
38	579254.0
39	727228.0
40	487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.884058701746792	18.040582870732838	24.737135873664062	27.338222553856312
2	30.41705552124813	21.0387429926657	28.161773558579583	20.382427927506587
3	27.245627845137356	22.07031401857973	31.511864256628236	19.17219387965468
4	24.804755928122944	24.66171712999755	28.40977658758105	22.123750354298455
5	23.432634286477665	33.589119344212705	26.362491085108676	16.615755284200965
6	84.16679958994445	2.1535691490452025	12.350791166417666	1.328840094592681
7	79.5768115518614	7.497084915944974	11.475028997694086	1.4510745344995397
8	79.1223199663172	3.5427960727591787	14.396238911312413	2.938645049611208
9	49.35575994456569	16.861826311646457	19.881049962738288	13.901363781049561
10	32.37212329091491	17.341386795429074	27.03701879908197	23.249471114574053
11	28.689257105348037	14.065466108333924	36.25054573153707	20.994731054780964
12	23.82883597141863	16.65305233862898	36.98918291177609	22.5289287781763
13	19.1807936426782	20.215427597888276	41.44932630870134	19.154452450732194
14	20.336908085968414	17.298317297327735	42.219817953619234	20.14495666308461
15	26.450326472921464	15.340469330299932	38.05416342801162	20.15504076876698
16	25.58179752953547	15.725031883915921	34.114317474506414	24.57885311204219
17	25.436944534827038	19.018505511976876	35.13488551594926	20.40966443724682
18	27.097594116159947	22.769415849905155	30.024364424500323	20.108625609434576
19	28.101410290452872	20.49983236352353	29.30568340667478	22.093073939348816
20	27.335300990527774	22.437912656094532	33.23530388853011	16.991482464847586
21	24.922749389004508	17.993508003552055	33.564851519790174	23.518891087653262
22	26.837739906882234	19.08772771406754	32.127206752204074	21.94732562684615
23	27.173554762701908	18.58441774774327	32.703367313785094	21.53866017576973
24	29.190658631110928	16.016104411018723	30.634806233202482	24.158430724667866
25	21.34213791993644	18.729859767316327	32.69582779551789	27.23217451722933
26	20.07052748119067	19.915778868756544	33.867704544185464	26.145989105867322
27	20.892664826238843	22.378468266756638	33.079047372442545	23.649819534561978
28	25.7073776306734	22.92890022225086	30.390973500242563	20.97274864683318
29	23.679412143760707	19.087916202024218	35.61604814736366	21.616623506851422
30	29.10023153389379	19.6675873517984	30.336288431810832	20.895892682496985
31	31.71241544253556	19.449224053984835	29.373963168982044	19.46439733449756
32	31.029452892500842	19.062423205883274	28.277198871051386	21.630925030564498
33	23.496578825781288	21.204070491668478	33.87986201739131	21.41948866515892
34	23.64628538537423	24.320106269509974	31.25490804968448	20.77870029543131
35	28.0083679228368	26.53005687859703	26.47770434862921	18.983870849936963
36	26.88250579659369	24.71411678195454	26.94414135842799	21.459236063023777
37	25.581467675611286	29.087720645769167	24.995694228239596	20.335117450379954
38	25.091281183270848	23.525393922158717	26.21144154882439	25.17188334574605
39	23.830626607007087	20.63601491222469	31.203474398505477	24.329884082262744
40	21.19565921660164	22.736972360361644	30.99041232447354	25.07695609856318
41	21.97790779781745	25.246359649629163	26.53262502700679	26.2431075255466
42	20.447032174658595	20.35601605257683	29.062486820568655	30.134464952195923
43	22.0763220721989	19.402337674760734	32.29095566456966	26.230384588470713
44	26.129779141592863	21.595324367746603	29.362865940532522	22.91203055012802
45	24.710535510777625	23.693430935538768	29.220086313347565	22.375947240336046
46	22.619426558377196	23.67217891842312	29.237191595416256	24.47120292778343
47	22.47306566001532	24.052312005057132	31.59600056829119	21.878621766636357
48	25.335585136122468	23.195587119958212	30.036262726765738	21.432565017153582
49	22.151999986805844	22.352127074810635	31.915770386798492	23.580102551585032
50	21.746044050106697	22.40824936391205	33.40392992677479	22.441776659206468
51	21.645132310299243	19.94553640491737	31.951583098567653	26.457748186215735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1983.0
1	16533.5
2	31084.0
3	102234.0
4	173384.0
5	106322.0
6	39260.0
7	34832.5
8	30405.0
9	29805.5
10	29206.0
11	28613.5
12	28021.0
13	26676.0
14	25331.0
15	23929.5
16	22528.0
17	21209.0
18	19890.0
19	19435.0
20	18980.0
21	17838.5
22	16697.0
23	17023.5
24	17350.0
25	17875.5
26	20194.0
27	21987.0
28	23463.5
29	24940.0
30	29188.0
31	33436.0
32	38519.5
33	43603.0
34	51874.0
35	60145.0
36	64973.0
37	69801.0
38	73625.0
39	77449.0
40	89373.0
41	101297.0
42	118235.0
43	135173.0
44	153247.0
45	171321.0
46	187052.5
47	202784.0
48	225343.0
49	247902.0
50	238828.5
51	229755.0
52	217675.5
53	205596.0
54	213148.0
55	220700.0
56	244313.5
57	267927.0
58	280832.5
59	293738.0
60	298566.5
61	303395.0
62	299710.0
63	296025.0
64	273120.5
65	250216.0
66	214967.0
67	179718.0
68	145989.0
69	112260.0
70	93722.0
71	75184.0
72	67765.5
73	60347.0
74	50362.5
75	31083.0
76	21788.0
77	17318.0
78	12848.0
79	9836.0
80	6824.0
81	4880.0
82	2936.0
83	2112.5
84	1289.0
85	863.0
86	437.0
87	351.5
88	266.0
89	189.5
90	113.0
91	105.0
92	97.0
93	92.0
94	87.0
95	53.5
96	20.0
97	10.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4244303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.93030552971423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22938832784875	16.936640819026692
2	9.826323814749445	4.309885669827247
3	3.0780714782981144	2.025091438841304
4	1.4859587400173335	1.3035011669251726
5	0.8494758967846405	0.9314632978307579
6	0.551758994176617	0.7260145992636614
7	0.4243211439386112	0.6513844630504115
8	0.327042566594379	0.5737714725309315
9	0.2603842400100483	0.5139275344688519
>10	3.7771214607196084	20.893373239948446
>50	1.5578372600425672	24.442398193835537
>100	0.6206004033507309	19.375967873372215
>500	0.007655786623427782	1.092326846009948
>1k	0.003247909476605606	1.3048904958762135
>5k	2.3199353404325753E-4	0.31309638613623747
>10k+	5.799838351081438E-4	4.606266503056484
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106252	2.5034027966429355	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	36712	0.8649712332036615	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	15708	0.37009610294081263	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12310	0.29003584334106214	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10093	0.23780111834616896	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6708	0.15804715167602312	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5600	0.13194156967586904	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4949	0.11660336220104928	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4421	0.10416315706018162	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0360718827095992	0.0
2	0.0	0.0	0.0	0.1207029752588352	0.0
3	0.0	0.0	0.0	0.18917122552277724	0.0
4	0.0	0.0	0.0	0.23674087358984502	0.0
5	0.0	0.0	0.0	0.5226064208893663	0.0
6	0.0	0.0	0.0	0.6156723495000239	0.0
7	0.0	0.0	0.0	0.8798853427759517	0.0
8	0.0	0.0	0.0	1.1873563221098964	0.0
9	0.0	0.0	0.0	1.4393882811853913	0.0
10	0.0	0.0	0.0	1.6223394041377348	0.0
11	0.0	0.0	0.0	1.8048664291875485	0.0
12	0.0	0.0	0.0	1.953442061040411	0.0
13	0.0	0.0	0.0	2.033997101526446	0.0
14	0.0	0.0	0.0	2.0891298288552913	0.0
15	0.0	0.0	0.0	2.2582035259970836	0.0
16	0.0	0.0	0.0	2.443039528516225	0.0
17	0.0	0.0	0.0	2.7006790043029443	4.712198916995323E-5
18	0.0	0.0	0.0	2.8508096617984156	4.712198916995323E-5
19	0.0	0.0	0.0	3.018728870205544	4.712198916995323E-5
20	0.0	0.0	0.0	3.2295055277627447	4.712198916995323E-5
21	0.0	0.0	0.0	3.4369129630942936	4.712198916995323E-5
22	4.712198916995323E-5	0.0	0.0	3.629853947750667	4.712198916995323E-5
23	7.068298375492984E-5	0.0	0.0	3.816362780885342	4.712198916995323E-5
24	9.424397833990646E-5	0.0	0.0	3.96941500170935	4.712198916995323E-5
25	9.424397833990646E-5	0.0	0.0	4.135991233425135	4.712198916995323E-5
26	9.424397833990646E-5	0.0	0.0	4.3004469756282715	4.712198916995323E-5
27	9.424397833990646E-5	0.0	0.0	4.475952824291762	4.712198916995323E-5
28	9.424397833990646E-5	0.0	0.0	4.655393359050945	4.712198916995323E-5
29	9.424397833990646E-5	0.0	0.0	4.840300044553841	4.712198916995323E-5
30	9.424397833990646E-5	0.0	0.0	5.046034649269856	4.712198916995323E-5
31	9.424397833990646E-5	0.0	0.0	5.244960126550814	4.712198916995323E-5
32	9.424397833990646E-5	0.0	0.0	5.478025484985403	4.712198916995323E-5
33	9.424397833990646E-5	0.0	0.0	5.697189856614855	4.712198916995323E-5
34	9.424397833990646E-5	0.0	0.0	5.946182447388888	4.712198916995323E-5
35	9.424397833990646E-5	0.0	0.0	6.173239752204307	4.712198916995323E-5
36	9.424397833990646E-5	0.0	0.0	6.432222204682371	4.712198916995323E-5
37	9.424397833990646E-5	0.0	0.0	6.779487703870341	4.712198916995323E-5
38	9.424397833990646E-5	0.0	0.0	7.203797655351185	4.712198916995323E-5
39	9.424397833990646E-5	0.0	0.0	7.650961771579456	4.712198916995323E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATA	30	2.1668893E-6	45.000004	15
CGTTTTT	33395	0.0	43.996105	1
ACGGGAT	550	0.0	41.727272	4
CCTACGG	65	0.0	41.538464	1
CGTTTTA	1790	0.0	40.977654	1
ACGTAGG	430	0.0	40.813953	1
CGCACTT	11545	0.0	40.790386	34
AACACGT	690	0.0	40.76087	40
CTTACTG	11530	0.0	40.745884	38
GCACTTA	11555	0.0	40.696667	35
AAACACG	710	0.0	40.56338	39
CACTTAC	11635	0.0	40.513535	36
CCGCACT	11625	0.0	40.509678	33
TAGTAGG	900	0.0	40.5	1
ACCCGCA	11625	0.0	40.490322	31
TAGCATA	4240	0.0	40.489388	29
ACTTACT	11645	0.0	40.40146	37
TATACGG	340	0.0	40.36765	1
TCACGAC	970	0.0	40.360825	24
CCCGCAC	11765	0.0	40.25712	32
>>END_MODULE