##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043467_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3093961
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.511685829265463	31.0	31.0	33.0	30.0	34.0
2	30.52489607981484	31.0	31.0	34.0	26.0	34.0
3	31.512183573096106	31.0	31.0	34.0	28.0	34.0
4	35.13632654063836	37.0	35.0	37.0	32.0	37.0
5	35.467452240025004	37.0	35.0	37.0	33.0	37.0
6	35.74627799122226	37.0	35.0	37.0	35.0	37.0
7	33.65186762211935	37.0	35.0	37.0	31.0	37.0
8	34.694921493839125	37.0	35.0	37.0	31.0	37.0
9	36.724328781132016	39.0	37.0	39.0	32.0	39.0
10	36.72665557193513	39.0	35.0	39.0	32.0	39.0
11	36.60263881800708	39.0	35.0	39.0	32.0	39.0
12	36.62712749126443	39.0	35.0	39.0	33.0	39.0
13	36.64140498215718	39.0	35.0	39.0	33.0	39.0
14	37.874433452781076	40.0	36.0	41.0	33.0	41.0
15	37.93656513446679	40.0	36.0	41.0	33.0	41.0
16	37.73728692766328	40.0	36.0	41.0	32.0	41.0
17	37.702655269410315	40.0	36.0	41.0	32.0	41.0
18	37.51677412869781	39.0	36.0	41.0	32.0	41.0
19	37.29939032844952	39.0	35.0	41.0	32.0	41.0
20	37.01687965685411	38.0	35.0	41.0	32.0	41.0
21	36.92997520007524	38.0	35.0	41.0	32.0	41.0
22	36.87256594378533	38.0	35.0	40.0	32.0	41.0
23	36.814450473034405	38.0	35.0	40.0	32.0	41.0
24	36.6890170884507	38.0	35.0	40.0	32.0	41.0
25	36.58639814787581	38.0	35.0	40.0	31.0	41.0
26	36.319733506660235	38.0	35.0	40.0	31.0	41.0
27	36.18744968019959	38.0	35.0	40.0	31.0	41.0
28	35.99494337517506	37.0	35.0	40.0	30.0	41.0
29	35.88725003320986	37.0	35.0	40.0	30.0	41.0
30	35.73223482778225	37.0	35.0	40.0	30.0	41.0
31	35.50144975970932	38.0	34.0	40.0	29.0	41.0
32	35.37173642460264	38.0	34.0	40.0	27.0	41.0
33	35.048955691425974	37.0	34.0	40.0	25.0	41.0
34	34.9166453617224	37.0	34.0	40.0	24.0	41.0
35	34.76722783512785	37.0	34.0	40.0	23.0	41.0
36	34.6380012547023	37.0	34.0	40.0	23.0	41.0
37	34.500092276534836	37.0	34.0	40.0	23.0	41.0
38	34.37598922546212	37.0	34.0	40.0	22.0	41.0
39	34.258287677187916	37.0	34.0	40.0	22.0	41.0
40	34.14834252920447	36.0	33.0	40.0	22.0	41.0
41	34.04802549224117	36.0	33.0	40.0	22.0	41.0
42	33.92097056168452	36.0	33.0	40.0	21.0	41.0
43	33.74000480290476	35.0	33.0	40.0	21.0	41.0
44	33.49976098599821	35.0	33.0	40.0	20.0	41.0
45	33.283313525930026	35.0	33.0	40.0	20.0	41.0
46	33.28233355236217	35.0	33.0	40.0	20.0	41.0
47	33.235991662467626	35.0	33.0	40.0	20.0	41.0
48	33.149639248846384	35.0	33.0	39.0	20.0	41.0
49	33.09895793773742	35.0	33.0	39.0	20.0	41.0
50	33.02058009134569	35.0	33.0	39.0	20.0	41.0
51	32.59069296607165	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	8.0
12	19.0
13	35.0
14	85.0
15	175.0
16	440.0
17	930.0
18	1832.0
19	3435.0
20	5832.0
21	9078.0
22	13908.0
23	20567.0
24	31310.0
25	46505.0
26	62124.0
27	73857.0
28	79085.0
29	82550.0
30	88367.0
31	100831.0
32	119250.0
33	155018.0
34	269090.0
35	292768.0
36	306896.0
37	338724.0
38	429124.0
39	561708.0
40	402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.844429196101697	16.889967262030776	22.50186734739061	30.76373619447692
2	33.355139253532926	19.945015467227933	25.977315163313307	20.72253011592583
3	26.17111204698443	21.21390670405994	33.47420992055168	19.140771328403947
4	23.0139940354775	23.774346218326606	30.836296902255718	22.37536284394018
5	20.880773868836744	33.48477889669586	29.25065959137817	16.38378764308923
6	80.47186115144956	2.1279841601106155	15.965844430488943	1.434310257950892
7	75.99685322471744	7.454812778829469	14.934803638442759	1.6135303580103306
8	75.22619063394788	3.8358272777194022	17.743727215695348	3.1942548726373734
9	40.46476345370869	21.596361427955944	23.82544576353742	14.113429354797944
10	27.01524033431578	18.906702443889888	32.62953217574494	21.448525046049387
11	24.618183616406284	15.419392810704466	40.47956648451613	19.482857088373123
12	21.803797785427808	16.995883270668248	40.401931375347004	20.79838756855694
13	18.387917624042448	21.227675461972535	41.41587434360032	18.968532570384696
14	18.984563800254755	19.760688644750207	40.87963616865242	20.37511138634262
15	23.41516263456456	15.620591209779308	40.64576121030614	20.31848494534999
16	22.246595868532278	16.816566207524914	37.60755226067814	23.329285663264663
17	22.350669578575815	20.469650393136824	36.52870866827345	20.650971360013912
18	23.688598531138563	24.05692896581437	33.15099964091338	19.103472862133685
19	24.97510472821086	20.66396441325537	32.92510797647417	21.4358228820596
20	25.548576727373103	22.207552066751973	35.21556994415896	17.02830126171597
21	21.893003822607977	18.474214768705878	36.195058696602835	23.437722712083314
22	22.874399515701718	21.10058918001875	35.1544185592514	20.870592745028137
23	24.018079090201848	20.61137810075822	36.06700278381014	19.303540025229797
24	26.11025155132854	18.155594075038437	33.96051210729547	21.773642266337554
25	20.188166560599825	20.000769240465537	34.79904885678908	25.012015342145556
26	19.05919305382324	19.43473107773498	37.30593242771968	24.2001434407221
27	20.574887660187056	21.675192415159724	36.86180918246868	20.888110742184534
28	22.913281712342204	22.805297157915046	34.29448528924573	19.98693584049702
29	21.920735264600943	19.211328132448987	38.84318515973536	20.0247514432147
30	26.90793452147587	20.461537815117904	33.160534344162706	19.46999331924352
31	26.185656509568155	21.753732513111835	32.098336081159395	19.96227489616062
32	26.614233340368543	20.089684388394037	30.96199338000705	22.334088891230365
33	21.71236159731813	22.217022127945373	33.836657928138074	22.233958346598424
34	21.384529410681	24.077872991934935	32.18515036227024	22.35244723511382
35	25.000250487966717	26.118137882151714	29.36821117008262	19.513400459798945
36	23.377347031846877	25.07410403686407	28.953887912614285	22.594661018674767
37	22.84531059053427	29.256412734355735	28.022040355389095	19.876236319720903
38	22.385156115413217	24.679205717201995	27.980184624175937	24.95545354320885
39	21.728586753355973	22.612179015831163	33.4686507037419	22.190583527070963
40	20.183512332573034	23.320429701602574	33.54405566198152	22.95200230384287
41	20.568585059734108	27.110102551389627	28.513223017355422	23.808089371520843
42	19.698406023863907	22.075391383407872	30.464799006839456	27.761403585888765
43	21.26587891702578	21.018913942354153	34.137502056425404	23.577705084194662
44	24.55040642076613	22.717868777272887	31.27437611527747	21.45734868668351
45	24.59132484216834	23.549036332390745	31.287401489546895	20.57223733589402
46	22.05774410213962	24.190447132332952	30.50494172357053	23.246867041956897
47	20.10270976266346	26.68640619581178	32.462723350423616	20.74816069110115
48	23.084841728774215	25.044756543472914	31.16726422860534	20.703137499147534
49	21.002139328840926	22.045138901233727	34.0486838715808	22.904037898344548
50	20.01418246707053	22.917612730089356	35.6037131689766	21.464491633863517
51	19.76818712323782	21.37421900276054	33.13752177225246	25.72007210174918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1337.0
1	14609.0
2	27881.0
3	105512.5
4	183144.0
5	111497.0
6	39850.0
7	35321.0
8	30792.0
9	30119.0
10	29446.0
11	28954.0
12	28462.0
13	27153.0
14	25844.0
15	24446.5
16	23049.0
17	21284.0
18	19519.0
19	18398.5
20	17278.0
21	16748.0
22	16218.0
23	16331.5
24	16445.0
25	16829.0
26	19615.0
27	22017.0
28	24044.5
29	26072.0
30	29502.5
31	32933.0
32	37408.0
33	41883.0
34	48649.5
35	55416.0
36	57273.0
37	59130.0
38	62622.5
39	66115.0
40	73350.5
41	80586.0
42	90631.0
43	100676.0
44	112131.0
45	123586.0
46	140086.0
47	156586.0
48	178727.0
49	200868.0
50	192144.5
51	183421.0
52	164561.5
53	145702.0
54	147910.5
55	150119.0
56	158458.5
57	166798.0
58	171957.5
59	177117.0
60	177781.5
61	178446.0
62	174373.0
63	170300.0
64	160034.5
65	149769.0
66	126307.5
67	102846.0
68	85513.0
69	68180.0
70	59657.0
71	51134.0
72	48368.5
73	45603.0
74	38274.5
75	23019.5
76	15093.0
77	11535.0
78	7977.0
79	6244.5
80	4512.0
81	3314.5
82	2117.0
83	1488.0
84	859.0
85	623.5
86	388.0
87	276.0
88	164.0
89	105.0
90	46.0
91	47.0
92	48.0
93	34.5
94	21.0
95	14.5
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3093961.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.020482910183414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.01208420998842	20.53883222363414
2	8.437596440392909	4.559758608413221
3	2.8793198474226127	2.334018381907038
4	1.5487215214016379	1.6738881360666484
5	1.1162431831518445	1.508071492698157
6	0.8456462918214139	1.3709862705732292
7	0.6962068125916712	1.316829099711056
8	0.5796882835490457	1.2530765887096436
9	0.5289962893197475	1.286436167560321
>10	6.664846879907118	40.027551889276616
>50	0.5853629146859094	10.225192804608161
>100	0.09501797507744249	4.3186011417975765
>500	0.006465887471609261	1.1876264474437614
>1k	0.002789206360302026	1.3256141978274973
>5k	5.07128429145823E-4	0.9067864331324649
>10k+	5.07128429145823E-4	6.1667301166405135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118985	3.8457175122763343	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	34333	1.1096778530821816	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	14354	0.46393603539281847	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12341	0.39887380610162826	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9865	0.31884694086318477	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6299	0.20359015514416634	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5197	0.16797238232802547	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5109	0.1651281318672084	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4945	0.15982748328114024	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	4035	0.13041534783405478	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04111234756999199	0.0
2	0.0	0.0	0.0	0.13461708146935272	0.0
3	0.0	0.0	0.0	0.21687409763730053	0.0
4	0.0	0.0	0.0	0.27256322881897993	0.0
5	0.0	0.0	0.0	0.5925737266888625	0.0
6	0.0	0.0	0.0	0.7383738838337005	0.0
7	0.0	0.0	0.0	1.086018860612658	0.0
8	0.0	0.0	0.0	1.465532370963952	0.0
9	0.0	0.0	0.0	1.8291439355570416	0.0
10	0.0	0.0	0.0	2.0551002420521782	0.0
11	0.0	0.0	0.0	2.2713279191302025	0.0
12	0.0	0.0	0.0	2.445182728547645	0.0
13	0.0	0.0	0.0	2.53981869842574	0.0
14	0.0	0.0	0.0	2.607369646870145	0.0
15	0.0	0.0	0.0	2.772271531541606	0.0
16	0.0	0.0	0.0	2.956404427851547	0.0
17	3.232102796383018E-5	0.0	0.0	3.2147787253944053	0.0
18	3.232102796383018E-5	0.0	0.0	3.3540500348905495	0.0
19	3.232102796383018E-5	0.0	0.0	3.517691399471422	0.0
20	3.232102796383018E-5	0.0	0.0	3.7011132331661583	0.0
21	3.232102796383018E-5	0.0	0.0	3.896461526179548	0.0
22	3.232102796383018E-5	0.0	0.0	4.093846043954659	0.0
23	3.232102796383018E-5	0.0	0.0	4.27561950522324	0.0
24	3.232102796383018E-5	0.0	0.0	4.429079745995505	0.0
25	6.464205592766037E-5	0.0	0.0	4.5773363012655945	0.0
26	6.464205592766037E-5	0.0	0.0	4.737131463518771	0.0
27	6.464205592766037E-5	0.0	0.0	4.918872603759388	0.0
28	9.696308389149056E-5	0.0	0.0	5.1034256734328585	0.0
29	9.696308389149056E-5	0.0	0.0	5.291695661322169	0.0
30	9.696308389149056E-5	0.0	0.0	5.47582855763211	0.0
31	9.696308389149056E-5	0.0	0.0	5.669948651582874	0.0
32	9.696308389149056E-5	0.0	0.0	5.895452463686517	0.0
33	9.696308389149056E-5	0.0	0.0	6.108835890303724	0.0
34	9.696308389149056E-5	0.0	0.0	6.3697958700836885	0.0
35	9.696308389149056E-5	0.0	0.0	6.593812914900996	0.0
36	9.696308389149056E-5	0.0	0.0	6.831372470435148	0.0
37	9.696308389149056E-5	0.0	0.0	7.1371617160009455	0.0
38	9.696308389149056E-5	0.0	0.0	7.443338813902308	0.0
39	9.696308389149056E-5	0.0	0.0	7.8149013513745	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTA	20	7.0351403E-4	45.0	37
CGTTTTT	32765	0.0	43.667786	1
CGTTTTA	1630	0.0	41.54908	1
AACACGT	620	0.0	41.370968	40
ACGTAGG	185	0.0	41.351353	1
CACTTAC	10280	0.0	41.125973	36
CGGTCTA	1005	0.0	40.97015	30
CTTACTG	10270	0.0	40.881207	38
ACTTACT	10285	0.0	40.821587	37
CGACGGT	1010	0.0	40.767326	27
ACGGGAT	740	0.0	40.743244	4
CGCACTT	10440	0.0	40.711205	34
GCACTTA	10440	0.0	40.474136	35
AGGGTAC	1315	0.0	40.38023	5
CCGCACT	10575	0.0	40.12766	33
CACGACG	1040	0.0	40.024036	25
TCACGAC	1035	0.0	39.782608	24
CCCGCAC	10725	0.0	39.776222	32
ACCCGCA	10675	0.0	39.751755	31
TAGGGTA	1280	0.0	39.55078	4
>>END_MODULE