##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043466_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3608805
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.519435658064097	31.0	31.0	33.0	30.0	34.0
2	30.554832139724923	31.0	31.0	34.0	26.0	34.0
3	31.544477465532218	31.0	31.0	34.0	28.0	34.0
4	35.15568643913983	37.0	35.0	37.0	32.0	37.0
5	35.47741288321203	37.0	35.0	37.0	33.0	37.0
6	35.744361083516566	37.0	35.0	37.0	35.0	37.0
7	33.69095531623349	37.0	35.0	37.0	32.0	37.0
8	34.703908357475676	37.0	35.0	37.0	31.0	37.0
9	36.70315713927464	39.0	37.0	39.0	32.0	39.0
10	36.70129724382448	39.0	35.0	39.0	32.0	39.0
11	36.45442244731982	38.0	35.0	39.0	32.0	39.0
12	36.42692387092126	39.0	35.0	39.0	32.0	39.0
13	36.46022048849966	39.0	35.0	39.0	32.0	39.0
14	37.675256490722	40.0	35.0	41.0	33.0	41.0
15	37.74337239058359	40.0	35.0	41.0	33.0	41.0
16	37.497621234730055	40.0	35.0	41.0	32.0	41.0
17	37.506134579175104	40.0	35.0	41.0	32.0	41.0
18	37.39653735793428	39.0	35.0	41.0	32.0	41.0
19	37.238947241538405	39.0	35.0	41.0	32.0	41.0
20	37.04944877875086	39.0	35.0	41.0	32.0	41.0
21	36.94488230868667	38.0	35.0	41.0	32.0	41.0
22	36.91361406338109	38.0	35.0	41.0	32.0	41.0
23	36.86947756944473	38.0	35.0	40.0	32.0	41.0
24	36.74949851820755	38.0	35.0	40.0	32.0	41.0
25	36.624786875433834	38.0	35.0	40.0	31.0	41.0
26	36.373636148254064	38.0	35.0	40.0	31.0	41.0
27	36.26074309916995	38.0	35.0	40.0	31.0	41.0
28	36.09282047658436	38.0	35.0	40.0	30.0	41.0
29	35.977531620578006	38.0	35.0	40.0	30.0	41.0
30	35.85356066620391	38.0	34.0	40.0	30.0	41.0
31	35.67552361515793	38.0	34.0	40.0	29.0	41.0
32	35.60822793140666	38.0	35.0	40.0	29.0	41.0
33	35.34371128392917	38.0	34.0	40.0	27.0	41.0
34	35.30419792701462	38.0	34.0	40.0	27.0	41.0
35	35.20668088189858	37.0	34.0	40.0	27.0	41.0
36	35.115622761551265	37.0	34.0	40.0	26.0	41.0
37	35.009310284152235	37.0	34.0	40.0	26.0	41.0
38	34.92129389091403	37.0	34.0	40.0	25.0	41.0
39	34.79572573192511	37.0	34.0	40.0	25.0	41.0
40	34.71572445726494	37.0	34.0	40.0	25.0	41.0
41	34.63040119928896	36.0	34.0	40.0	24.0	41.0
42	34.523889486963135	36.0	34.0	40.0	24.0	41.0
43	34.34698549796955	36.0	33.0	40.0	23.0	41.0
44	34.12245909657075	35.0	33.0	40.0	23.0	41.0
45	33.91189022404924	35.0	33.0	40.0	23.0	41.0
46	33.914398256486564	35.0	33.0	40.0	23.0	41.0
47	33.88304798956995	35.0	33.0	40.0	23.0	41.0
48	33.784743703248026	35.0	33.0	39.0	23.0	41.0
49	33.75276663604711	35.0	33.0	39.0	23.0	41.0
50	33.69231975681701	35.0	33.0	39.0	23.0	41.0
51	33.25665393391996	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	15.0
12	17.0
13	28.0
14	73.0
15	171.0
16	335.0
17	850.0
18	1754.0
19	3289.0
20	5531.0
21	8854.0
22	13574.0
23	20172.0
24	31250.0
25	45093.0
26	60321.0
27	71838.0
28	79837.0
29	87513.0
30	98427.0
31	116041.0
32	140485.0
33	186230.0
34	343672.0
35	368603.0
36	343030.0
37	401950.0
38	507718.0
39	671764.0
40	363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.842645972835882	17.580057664517756	23.85975412913693	26.717542233509427
2	30.36675575432865	21.747143444990794	28.246691079179946	19.63940972150061
3	28.586221754846825	21.610643966631613	30.448250875289744	19.354883403231817
4	24.14926824807658	24.37873478893983	27.740678701121286	23.731318261862306
5	21.362833403301092	35.18028821174877	26.599885557684605	16.856992827265536
6	84.42750439549934	2.234728670571006	11.928519274385842	1.4092476595438104
7	79.80184576334825	7.514842170746272	11.084915920921192	1.5983961449842814
8	79.41797908171819	3.709787588966431	13.728422566472837	3.143810762842548
9	44.75584022966051	19.277877302874497	20.42534855720938	15.54093391025561
10	31.351874096827064	18.84720842494953	27.139704140290206	22.661213337933194
11	28.57023308269635	15.761256149888952	36.55506462665619	19.113446140758505
12	21.79050960082354	19.1592230669155	36.88112824051174	22.16913909174921
13	18.221516540793974	22.8678468357254	39.76549023845844	19.145146385022187
14	20.759558912160674	19.878048273597493	38.92371574523977	20.438677069002065
15	25.197066619005458	15.324518781147775	38.875722018784614	20.60269258106215
16	22.452473879857738	16.91449108499905	35.13855694613591	25.494478089007305
17	24.639291953984767	20.899716111011816	33.45511879971348	21.00587313528994
18	24.990710221250527	25.39455581556776	29.459363972284454	20.155369990897263
19	27.726768279250336	21.151877144927475	28.979592967755252	22.141761608066936
20	26.858863252517107	24.421934684750216	31.078071550000626	17.641130512732055
21	22.374082279314067	19.317918258260004	34.0668171319869	24.241182330439024
22	25.676643653508574	21.535355886505368	31.15768239070828	21.63031806927778
23	27.188168936808722	20.604964801367764	32.321447127234634	19.885419134588876
24	26.942159523720456	17.595187326552693	30.940574511507275	24.522078638219575
25	21.058660692389864	20.729936918176513	32.800774771704205	25.410627617729414
26	19.543893338653653	20.663072679183276	33.863093184586035	25.92994079757704
27	20.44890206037733	24.114963263462556	33.955783147052834	21.48035152910728
28	24.331018162521943	23.295412193232938	31.250898843245896	21.12267080099922
29	23.32941236780596	20.521114330089876	35.85103656196441	20.298436740139742
30	30.388868337302792	20.257619904649875	29.950884018393904	19.40262773965343
31	29.641972896845353	22.12768492617362	28.738793035367667	19.49154914161336
32	28.63995145207347	20.13428267806102	28.483583901042035	22.742181968823473
33	22.817303788927358	22.193828705069958	32.557896589037085	22.430970916965588
34	22.80134282678061	24.3897910804269	30.08425226633193	22.724613826460562
35	26.83774822967714	27.137681310018134	26.181686181436792	19.84288427886794
36	25.701499526851684	24.619119071271513	26.87546154474958	22.80391985712722
37	24.079882398744182	29.354065958121872	25.697536996318725	20.86851464681522
38	25.470619775798358	23.83689337606216	25.636131628059704	25.05635522007978
39	23.25567604788843	21.332019879156675	30.809589323889764	24.602714749065132
40	20.141376439015133	23.814115752998568	31.349574166517723	24.694933641468573
41	21.066308653418513	26.385188448807845	26.210255195279323	26.338247702494318
42	20.76169258244765	20.656505408299978	28.859802621643453	29.721999387608918
43	21.976831665883857	20.851500704526845	31.891415579395392	25.2802520501939
44	25.365349471639504	23.078692254084107	29.18392653523812	22.37203173903827
45	24.803584566082122	23.432909231726292	29.352569617920615	22.41093658427097
46	22.77285694294926	23.61416036610457	29.211442568939027	24.401540122007145
47	21.2209858942226	25.677114723571933	31.54027441216691	21.561624970038558
48	23.54749009713742	24.970398788518636	29.96036083966853	21.521750274675412
49	21.055917402020892	21.885721173629495	33.17269844172794	23.88566298262167
50	20.24947316355414	22.842409052304017	34.421837699737175	22.486280084404672
51	20.000665040089448	20.383257061548075	32.86622580050737	26.749852097855108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1847.0
1	13595.5
2	25344.0
3	83200.5
4	141057.0
5	86715.0
6	32373.0
7	28945.0
8	25517.0
9	25001.0
10	24485.0
11	24039.5
12	23594.0
13	22556.5
14	21519.0
15	20144.0
16	18769.0
17	17217.5
18	15666.0
19	14906.5
20	14147.0
21	14130.0
22	14113.0
23	14243.0
24	14373.0
25	16073.0
26	19624.0
27	21475.0
28	23962.5
29	26450.0
30	29378.5
31	32307.0
32	36267.0
33	40227.0
34	48156.5
35	56086.0
36	63133.5
37	70181.0
38	71009.5
39	71838.0
40	83855.5
41	95873.0
42	112231.0
43	128589.0
44	142494.0
45	156399.0
46	171048.5
47	185698.0
48	215271.0
49	244844.0
50	239784.0
51	234724.0
52	215415.5
53	196107.0
54	196034.5
55	195962.0
56	209946.0
57	223930.0
58	232716.0
59	241502.0
60	242550.5
61	243599.0
62	233987.5
63	224376.0
64	199724.0
65	175072.0
66	141097.5
67	107123.0
68	89977.0
69	72831.0
70	64347.5
71	55864.0
72	53063.5
73	50263.0
74	41524.0
75	25116.5
76	17448.0
77	13177.5
78	8907.0
79	6705.5
80	4504.0
81	3265.0
82	2026.0
83	1377.0
84	728.0
85	542.5
86	357.0
87	221.5
88	86.0
89	59.0
90	32.0
91	25.0
92	18.0
93	13.5
94	9.0
95	7.5
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3608805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.274733779817353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.66111363140848	17.298806311837687
2	9.869257584140183	4.396701705823319
3	3.202437133631904	2.140003037947561
4	1.4027296989307712	1.249817224349051
5	0.8055390602640424	0.8971584058312897
6	0.5368603706971437	0.717505310052776
7	0.3931784616977446	0.6130561893592764
8	0.3281160965881652	0.5846958960299374
9	0.25790013211937973	0.5170191106265016
>10	3.336917762261428	19.095082757036334
>50	1.599353392803924	25.36888139567026
>100	0.593181840194096	19.004256990007395
>500	0.008585494568219198	1.2713848048087353
>1k	0.003756153873595899	1.5000954209600483
>5k	5.365934105136999E-4	0.9590965046522981
>10k+	5.365934105136999E-4	4.386438935007594
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85524	2.3698703587475634	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	34889	0.9667743200311459	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	14247	0.3947844230985049	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12136	0.3362886052308174	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9378	0.2598644149517638	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8073	0.22370286008803467	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7784	0.21569466901093298	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	6862	0.19014604557464312	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3756	0.10407877399859511	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03763018506125989	0.0
2	0.0	0.0	0.0	0.12893464734171006	0.0
3	0.0	0.0	0.0	0.19926263680082465	0.0
4	0.0	0.0	0.0	0.2450672729615482	0.0
5	0.0	0.0	0.0	0.5263792307980065	0.0
6	0.0	0.0	0.0	0.5934928598247896	0.0
7	0.0	0.0	0.0	0.8225160406284074	0.0
8	0.0	0.0	0.0	1.0748987545738824	0.0
9	0.0	0.0	0.0	1.2947776341475918	0.0
10	0.0	0.0	0.0	1.459541316308307	0.0
11	0.0	0.0	0.0	1.6401274105971366	0.0
12	0.0	0.0	0.0	1.7960793115726674	0.0
13	0.0	0.0	0.0	1.872558921859175	0.0
14	0.0	0.0	0.0	1.92831144935789	0.0
15	2.7710003726995503E-5	0.0	0.0	2.112610684146137	0.0
16	2.7710003726995503E-5	0.0	0.0	2.3243705326278365	0.0
17	2.7710003726995503E-5	0.0	0.0	2.6034656901661353	0.0
18	2.7710003726995503E-5	0.0	0.0	2.7673149422038597	0.0
19	2.7710003726995503E-5	0.0	0.0	2.9458504962168917	0.0
20	2.7710003726995503E-5	0.0	0.0	3.146360083185431	0.0
21	2.7710003726995503E-5	0.0	0.0	3.347035930176333	0.0
22	5.5420007453991005E-5	0.0	0.0	3.5649473994854253	0.0
23	1.3855001863497752E-4	0.0	0.0	3.7562018452091483	0.0
24	1.66260022361973E-4	0.0	0.0	3.918804147079158	0.0
25	1.66260022361973E-4	0.0	0.0	4.080436598818722	0.0
26	1.66260022361973E-4	0.0	0.0	4.247029141225419	0.0
27	1.66260022361973E-4	0.0	0.0	4.445488187918161	0.0
28	1.66260022361973E-4	0.0	0.0	4.62995368272877	0.0
29	1.66260022361973E-4	0.0	0.0	4.833289690077463	0.0
30	1.66260022361973E-4	0.0	0.0	5.03806661761996	0.0
31	1.66260022361973E-4	0.0	0.0	5.2435917152630855	0.0
32	1.66260022361973E-4	0.0	0.0	5.4993827596669815	0.0
33	1.66260022361973E-4	0.0	0.0	5.729070980560047	0.0
34	1.66260022361973E-4	0.0	0.0	6.005699947766643	0.0
35	1.66260022361973E-4	0.0	0.0	6.237743518976504	0.0
36	1.66260022361973E-4	0.0	0.0	6.483946902090858	0.0
37	1.66260022361973E-4	0.0	0.0	6.828354538413685	0.0
38	2.4939003354295953E-4	0.0	0.0	7.136572909869057	0.0
39	2.4939003354295953E-4	0.0	0.0	7.566133387644941	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	35	1.2129021E-7	45.0	16
TACGAAC	20	7.035297E-4	45.0	44
CGATATA	25	3.8924714E-5	45.0	9
CGTTTTT	26195	0.0	43.866196	1
CGGTCTA	1065	0.0	43.098595	30
TCACGAC	1105	0.0	41.742085	24
CACGACG	1095	0.0	41.712326	25
CATGCGG	565	0.0	41.41593	1
CGGGATA	420	0.0	41.25	5
CGCACTT	12735	0.0	41.024734	34
ATCACGG	225	0.0	41.0	1
GCACTTA	12805	0.0	40.905895	35
TACGCGG	380	0.0	40.855263	1
ACGGTCT	1120	0.0	40.78125	29
ATTGCGG	675	0.0	40.666668	1
ACCCGCA	12890	0.0	40.65361	31
CACTTAC	12865	0.0	40.64516	36
CCGCACT	12835	0.0	40.634983	33
CGTTTTA	1275	0.0	40.588234	1
CCCGCAC	13020	0.0	40.42051	32
>>END_MODULE