FastQCFastQC Report
Thu 26 May 2016
SRR1043465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043465_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1168204
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720576.168186378406512No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77890.6667499854477472No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.261255739579731No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.11864366155226312No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12090.1034921982804373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCAC453.8562575E-1045.00000444
ACACGAT406.8157533E-945.020
CGTTGAC207.0333347E-445.038
CGGCACG351.2119563E-745.028
GTGATCG207.0333347E-445.034
TCGATCC253.8908456E-545.020
TTATCGG253.8908456E-545.01
AGAACGC253.8908456E-545.07
CCATGCG253.8908456E-545.033
ATGTACG351.2119563E-745.028
ACTAGTC253.8908456E-545.017
GTTCGCA502.1827873E-1145.017
ACAATCG207.0333347E-445.022
ACGTGAC207.0333347E-445.038
TTGCGAC351.2119563E-745.023
TTACGCG207.0333347E-445.01
ACATCGC207.0333347E-445.017
CACGTTG207.0333347E-445.036
CCAACCG351.2119563E-745.031
GTGACGG800.045.01