##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043465_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1168204
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52974052477136	31.0	31.0	33.0	30.0	34.0
2	30.581918911423006	31.0	31.0	34.0	26.0	34.0
3	31.47330089607637	31.0	31.0	34.0	28.0	34.0
4	35.090824034158416	37.0	35.0	37.0	32.0	37.0
5	35.39363415978716	37.0	35.0	37.0	33.0	37.0
6	35.83305227511633	37.0	35.0	37.0	35.0	37.0
7	33.82663901167947	37.0	35.0	37.0	32.0	37.0
8	34.81584723216151	37.0	35.0	37.0	32.0	37.0
9	36.77009922924421	39.0	37.0	39.0	32.0	39.0
10	36.74896507801719	39.0	35.0	39.0	32.0	39.0
11	36.51408914881305	39.0	35.0	39.0	32.0	39.0
12	36.43645887190936	39.0	35.0	39.0	32.0	39.0
13	36.47615912974104	39.0	35.0	39.0	32.0	39.0
14	37.835424292332505	40.0	36.0	41.0	33.0	41.0
15	37.99078927995453	40.0	36.0	41.0	33.0	41.0
16	37.94677898723168	40.0	36.0	41.0	33.0	41.0
17	37.90324207073422	40.0	36.0	41.0	33.0	41.0
18	37.594347391380275	39.0	36.0	41.0	33.0	41.0
19	37.265499005310716	38.0	35.0	41.0	33.0	41.0
20	36.92715484624261	38.0	35.0	41.0	33.0	41.0
21	36.83944413818134	38.0	35.0	41.0	32.0	41.0
22	36.7591533670489	38.0	35.0	40.0	32.0	41.0
23	36.65604808749157	38.0	35.0	40.0	32.0	41.0
24	36.48940339187334	37.0	35.0	40.0	32.0	41.0
25	36.39568602744041	37.0	35.0	40.0	32.0	41.0
26	36.103059054754134	37.0	35.0	40.0	31.0	41.0
27	36.02263988139058	36.0	35.0	40.0	31.0	41.0
28	35.94746123108635	36.0	35.0	40.0	31.0	41.0
29	35.868524675484764	36.0	35.0	40.0	31.0	41.0
30	35.699726246443255	36.0	35.0	40.0	30.0	41.0
31	35.18761021191504	36.0	34.0	40.0	28.0	41.0
32	34.98800380755416	36.0	34.0	40.0	26.0	41.0
33	34.7933306169128	36.0	34.0	40.0	24.0	41.0
34	34.53816627917727	36.0	34.0	40.0	23.0	41.0
35	34.28430907615451	36.0	34.0	40.0	21.0	41.0
36	34.105697292596155	36.0	34.0	40.0	21.0	41.0
37	33.99867745701949	36.0	33.0	40.0	20.0	41.0
38	33.90681850087827	36.0	33.0	40.0	20.0	41.0
39	33.78235393818203	36.0	33.0	40.0	18.0	41.0
40	33.71489226196795	36.0	33.0	40.0	18.0	41.0
41	33.6304018818631	35.0	33.0	40.0	18.0	41.0
42	33.55294109590448	35.0	33.0	40.0	18.0	41.0
43	33.387427195934954	35.0	33.0	40.0	18.0	41.0
44	33.20690393116271	35.0	33.0	40.0	17.0	41.0
45	33.04871238242636	35.0	33.0	40.0	15.0	41.0
46	33.04503408651229	35.0	33.0	40.0	16.0	41.0
47	33.018488209251124	35.0	33.0	40.0	16.0	41.0
48	32.95608900500255	35.0	33.0	40.0	15.0	41.0
49	32.86333551331788	35.0	33.0	40.0	15.0	41.0
50	32.743166433259944	35.0	33.0	40.0	15.0	41.0
51	32.30628469000278	35.0	31.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	11.0
13	12.0
14	20.0
15	50.0
16	148.0
17	321.0
18	702.0
19	1318.0
20	2222.0
21	3645.0
22	5669.0
23	8945.0
24	14490.0
25	21921.0
26	27580.0
27	29875.0
28	29572.0
29	29664.0
30	31720.0
31	36611.0
32	45035.0
33	57685.0
34	95724.0
35	136692.0
36	125645.0
37	101966.0
38	137201.0
39	223637.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.370486661576233	18.6045416725161	25.01960274061722	32.00536892529045
2	33.150203217931114	20.15803746605901	28.740100187980865	17.95165912802901
3	20.594177044420324	20.741754008717656	40.55207823291138	18.111990713950647
4	21.810231774587315	24.429979695327187	37.33371911070327	16.42606941938223
5	18.972628068385315	30.23821181916857	35.10020510116384	15.688955011282276
6	75.60229206542692	2.1040845605733245	20.729684198992643	1.563939175007105
7	72.24243368452771	7.370630472075083	18.58725017205899	1.7996856713382252
8	70.62345275311503	4.5428709369254	21.966026481676145	2.867649828283416
9	37.90057216034185	22.047262293229608	28.48440854508288	11.567757001345655
10	28.190281834337156	23.907896223604784	37.370270945827954	10.53155099623011
11	27.000335557830653	21.03879117003537	39.39175007104924	12.569123201084743
12	18.61977873727534	28.93989405959918	37.275766903725724	15.164560299399762
13	13.383706955292055	30.992532126238224	37.78021646904136	17.843544449428354
14	13.184341091110799	24.228045786523587	45.6284176393849	16.959195482980714
15	15.057472838648046	21.614033165440283	45.66274383583689	17.66575016007478
16	16.162160033692746	23.739432496379056	38.275078667766934	21.823328802161264
17	17.441474263056794	23.95326501193285	41.714204026009156	16.891056699001204
18	20.435643089734327	25.956168614385845	37.63623476721531	15.971953528664514
19	21.116602922092376	25.191148121389755	37.609355900168126	16.082893056349747
20	16.04325956767825	28.39324296098969	38.38559018801511	17.177907283316955
21	16.47580388356828	26.27871501895217	37.07451780682141	20.17096329065814
22	14.275931258581547	28.615635625284625	37.239471873063266	19.868961243070558
23	13.399885636412817	25.598097592543766	45.77582340070741	15.226193370336002
24	14.775587140602156	26.288559190004484	42.03897606924818	16.89687760014518
25	15.093083057411205	29.40239889608322	38.37514680655091	17.129371239954665
26	14.047546490167814	30.520867930601163	37.75231038414523	17.67927519508579
27	14.209846910299914	28.25131569486151	41.94721127474311	15.591626120095462
28	13.387815826687804	25.331277756282294	42.30887755905647	18.972028857973434
29	14.339105156291195	30.015904756361046	39.90227734197109	15.742712745376664
30	19.50438450818521	28.515310682038407	37.025382553047244	14.954922256729134
31	18.997538101222048	27.945718384802653	37.235363001667515	15.821380512307782
32	18.191600097243292	29.377831269196136	36.485921979380315	15.944646654180264
33	16.3323357906667	28.207230928844616	37.0250401470976	18.435393133391088
34	16.014839873857646	27.648338817535294	38.86298968330873	17.473831625298324
35	20.771800130799072	28.42414509794522	35.49756720572776	15.306487565527938
36	17.37787235791009	30.88373263573828	36.249833077099545	15.488561929252082
37	17.563028375181048	29.934326538857942	36.66260344939754	15.840041636563477
38	16.84782794785842	31.753614950813386	35.218506356766454	16.18005074456174
39	20.067727896839934	28.45949851224615	34.58676737966999	16.886006211243927
40	16.21446254250114	27.9012056113487	36.43019541107546	19.454136435074695
41	17.02134216284142	28.572321272654435	34.11356235725952	20.292774207244623
42	17.580919086050038	27.944006355054423	35.50261769348504	18.972456865410496
43	17.21985201214856	28.680692755717324	36.28321765719001	17.8162375749441
44	14.606866608914196	33.250271356715096	34.507842808276635	17.635019226094073
45	17.445583134452544	31.01059404008204	34.411284330476526	17.13253849498889
46	17.06885098835477	29.690790307172378	34.43807759603631	18.802281108436542
47	16.239714981287516	27.941609513406906	37.299735320200924	18.518940185104658
48	17.437964602072924	27.49776580117856	36.49996062331579	18.564308973432723
49	17.345514995668566	29.28743609848965	35.72826321430161	17.638785691540175
50	16.04283156024119	29.089439858106974	35.62862308295469	19.239105498697146
51	16.024684044909964	27.54929789660025	36.90879332719285	19.51722473129693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1037.0
1	7711.0
2	14385.0
3	53552.0
4	92719.0
5	55505.5
6	18292.0
7	17926.5
8	17561.0
9	19698.5
10	21836.0
11	23423.0
12	25010.0
13	24221.5
14	23433.0
15	20597.5
16	17762.0
17	15402.0
18	13042.0
19	11663.5
20	10285.0
21	9505.0
22	8725.0
23	8180.5
24	7636.0
25	8494.0
26	10296.5
27	11241.0
28	12240.5
29	13240.0
30	15692.0
31	18144.0
32	20767.5
33	23391.0
34	29293.0
35	35195.0
36	38638.0
37	42081.0
38	45092.5
39	48104.0
40	52352.0
41	56600.0
42	64866.5
43	73133.0
44	78841.0
45	84549.0
46	86810.5
47	89072.0
48	89369.0
49	89666.0
50	84670.0
51	79674.0
52	69507.0
53	59340.0
54	52358.5
55	45377.0
56	40051.0
57	34725.0
58	32220.5
59	29716.0
60	24369.5
61	19023.0
62	15698.5
63	12374.0
64	10219.5
65	8065.0
66	6460.0
67	4855.0
68	4080.0
69	3305.0
70	2804.5
71	2304.0
72	2016.5
73	1729.0
74	1431.0
75	827.0
76	521.0
77	394.0
78	267.0
79	226.5
80	186.0
81	124.0
82	62.0
83	49.0
84	36.0
85	23.0
86	10.0
87	8.0
88	6.0
89	3.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1168204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.81395524997065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.29257678084856	26.811956429224747
2	9.248715925741381	6.254713328654198
3	3.3520530560246335	3.4003851609583293
4	1.7126008883036308	2.3163923919263576
5	1.0297414736030717	1.7409816053726546
6	0.6827091249719496	1.385105748032887
7	0.4834553938042754	1.1443277336018292
8	0.3936802675812874	1.0649509560632098
9	0.2782416570894856	0.846760584735139
>10	2.627441572903342	19.87645654279458
>50	0.6820435341014518	16.222290907627592
>100	0.2107414953336865	10.262062380719856
>500	0.003912280234733351	0.8428615763703214
>1k	0.0015649120938933407	0.7888873915070131
>5k	2.6081868231555677E-4	0.6869361534318754
>10k+	2.6081868231555677E-4	6.354931108979413
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72057	6.168186378406512	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7789	0.6667499854477472	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3052	0.261255739579731	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	0.11864366155226312	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1209	0.1034921982804373	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1010097551455054	0.0
2	0.0	0.0	0.0	0.3162118944978788	0.0
3	0.0	0.0	0.0	0.5162625705784264	0.0
4	0.0	0.0	0.0	0.6463768314438232	0.0
5	0.0	0.0	0.0	1.4162766092223618	0.0
6	0.0	0.0	0.0	1.6126464213442173	0.0
7	0.0	0.0	0.0	2.233770814001664	0.0
8	0.0	0.0	0.0	2.9708852221016193	0.0
9	0.0	0.0	0.0	3.5309757542347056	0.0
10	0.0	0.0	0.0	3.929193873672749	0.0
11	0.0	0.0	0.0	4.363364617823599	0.0
12	0.0	0.0	0.0	4.715871542983931	0.0
13	0.0	0.0	0.0	4.8734638813084015	0.0
14	0.0	0.0	0.0	4.993477166659248	0.0
15	0.0	0.0	0.0	5.4311575717939675	0.0
16	0.0	0.0	0.0	5.94365367692629	0.0
17	0.0	0.0	0.0	6.573937428736762	0.0
18	0.0	0.0	0.0	6.895199810991916	0.0
19	0.0	0.0	0.0	7.260118951826907	0.0
20	0.0	0.0	0.0	7.681877480303098	0.0
21	0.0	0.0	0.0	8.112795367932312	0.0
22	8.560148741144527E-5	0.0	0.0	8.533355475584743	0.0
23	8.560148741144527E-5	0.0	0.0	8.887232024543659	0.0
24	4.280074370572263E-4	0.0	0.0	9.204385535403063	0.0
25	4.280074370572263E-4	0.0	0.0	9.507072394889933	0.0
26	5.992104118801168E-4	0.0	0.0	9.851703983208413	0.0
27	6.848118992915621E-4	0.0	0.0	10.161238961688198	0.0
28	6.848118992915621E-4	0.0	0.0	10.505528144057031	0.0
29	7.704133867030074E-4	0.0	0.0	10.907683931916	0.0
30	7.704133867030074E-4	0.0	0.0	11.277311154558621	0.0
31	7.704133867030074E-4	0.0	0.0	11.607818497454211	0.0
32	7.704133867030074E-4	0.0	0.0	11.947570800990238	0.0
33	7.704133867030074E-4	0.0	0.0	12.311034716539234	0.0
34	7.704133867030074E-4	0.0	0.0	12.73544689112518	0.0
35	7.704133867030074E-4	0.0	0.0	13.094630732303605	0.0
36	7.704133867030074E-4	0.0	0.0	13.437464689386443	0.0
37	7.704133867030074E-4	0.0	0.0	13.814282436971626	0.0
38	7.704133867030074E-4	0.0	0.0	14.186306501261766	0.0
39	7.704133867030074E-4	0.0	0.0	14.599333678021988	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCAC	45	3.8562575E-10	45.000004	44
ACACGAT	40	6.8157533E-9	45.0	20
CGTTGAC	20	7.0333347E-4	45.0	38
CGGCACG	35	1.2119563E-7	45.0	28
GTGATCG	20	7.0333347E-4	45.0	34
TCGATCC	25	3.8908456E-5	45.0	20
TTATCGG	25	3.8908456E-5	45.0	1
AGAACGC	25	3.8908456E-5	45.0	7
CCATGCG	25	3.8908456E-5	45.0	33
ATGTACG	35	1.2119563E-7	45.0	28
ACTAGTC	25	3.8908456E-5	45.0	17
GTTCGCA	50	2.1827873E-11	45.0	17
ACAATCG	20	7.0333347E-4	45.0	22
ACGTGAC	20	7.0333347E-4	45.0	38
TTGCGAC	35	1.2119563E-7	45.0	23
TTACGCG	20	7.0333347E-4	45.0	1
ACATCGC	20	7.0333347E-4	45.0	17
CACGTTG	20	7.0333347E-4	45.0	36
CCAACCG	35	1.2119563E-7	45.0	31
GTGACGG	80	0.0	45.0	1
>>END_MODULE