FastQCFastQC Report
Thu 26 May 2016
SRR1043464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043464_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences598063
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424787.102596214780048No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47450.7933946758117457No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.30632224364322824No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.1366076817994091No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT7750.12958501027483726No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC7160.11971982884746254No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC6340.10600889872806044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGA453.8380676E-1045.0000041
ATACCGG453.8380676E-1045.0000041
TCACGAC800.045.024
GGTACTC253.8885533E-545.07
GTACCGG351.2106284E-745.01
GCCCAAA253.8885533E-545.012
CTACTTG207.030569E-445.040
GATAATC207.030569E-445.08
TACTTGC207.030569E-445.041
GATCGGC207.030569E-445.09
TTTACGG551.8189894E-1245.01
CTATCGG207.030569E-445.01
TAGACGG502.1827873E-1145.01
TCCGAAT406.8048394E-945.036
TGGACCG207.030569E-445.031
TAGGTCC207.030569E-445.032
ATGGACC207.030569E-445.030
CGGCAGT351.2106284E-745.024
GAATCCG207.030569E-445.08
GTGATCC253.8885533E-545.013