##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1043464_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 598063 Sequences flagged as poor quality 0 Sequence length 51 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.468357012555533 31.0 31.0 33.0 30.0 34.0 2 30.467735004506213 31.0 31.0 34.0 26.0 34.0 3 31.367272344217916 31.0 31.0 34.0 28.0 34.0 4 34.99962545751869 37.0 35.0 37.0 32.0 37.0 5 35.31749330756124 37.0 35.0 37.0 33.0 37.0 6 35.78169523946474 37.0 35.0 37.0 35.0 37.0 7 33.67338056358611 37.0 35.0 37.0 31.0 37.0 8 34.703507824426524 37.0 35.0 37.0 31.0 37.0 9 36.6692505639038 39.0 37.0 39.0 32.0 39.0 10 36.644938743911595 39.0 35.0 39.0 32.0 39.0 11 36.368100016219024 38.0 35.0 39.0 32.0 39.0 12 36.19226068156699 39.0 35.0 39.0 32.0 39.0 13 36.265983683993156 39.0 35.0 39.0 32.0 39.0 14 37.63568219401635 40.0 35.0 41.0 32.0 41.0 15 37.802825120430455 40.0 35.0 41.0 33.0 41.0 16 37.769517592628205 40.0 35.0 41.0 33.0 41.0 17 37.74205225870853 40.0 36.0 41.0 33.0 41.0 18 37.43046468348652 39.0 36.0 40.0 33.0 41.0 19 37.09547656350585 38.0 35.0 40.0 33.0 41.0 20 36.73466674915519 38.0 35.0 40.0 32.0 41.0 21 36.63993091028872 38.0 35.0 40.0 32.0 41.0 22 36.53759219346457 37.0 35.0 40.0 32.0 41.0 23 36.45494538200825 37.0 35.0 40.0 32.0 41.0 24 36.26747014946586 37.0 35.0 40.0 32.0 41.0 25 36.15581970461306 36.0 35.0 40.0 31.0 41.0 26 35.87409018782302 36.0 35.0 40.0 31.0 41.0 27 35.79915995472049 36.0 35.0 40.0 31.0 41.0 28 35.70907078351277 36.0 35.0 40.0 31.0 41.0 29 35.630348307786974 36.0 35.0 40.0 30.0 41.0 30 35.450287009896954 35.0 35.0 40.0 30.0 41.0 31 34.95870000317692 35.0 34.0 40.0 27.0 41.0 32 34.78063515047746 35.0 34.0 40.0 25.0 41.0 33 34.63139334819241 35.0 34.0 40.0 25.0 41.0 34 34.4266089024066 35.0 34.0 40.0 23.0 41.0 35 34.18487182788435 35.0 34.0 40.0 22.0 41.0 36 34.00108516995701 35.0 33.0 40.0 21.0 41.0 37 33.91905367829142 35.0 33.0 40.0 21.0 41.0 38 33.827462993029165 35.0 33.0 40.0 21.0 41.0 39 33.68812984585236 35.0 33.0 40.0 20.0 41.0 40 33.628774894952535 35.0 33.0 40.0 20.0 41.0 41 33.54260337121674 35.0 33.0 40.0 19.0 41.0 42 33.44964159294255 35.0 33.0 40.0 18.0 41.0 43 33.257648441719354 35.0 33.0 40.0 18.0 41.0 44 33.05657263532437 35.0 33.0 40.0 18.0 41.0 45 32.870659111163874 35.0 33.0 40.0 17.0 41.0 46 32.87840913081063 35.0 33.0 40.0 17.0 41.0 47 32.86180552884897 35.0 33.0 40.0 17.0 41.0 48 32.81025744779396 35.0 33.0 40.0 17.0 41.0 49 32.70976803447128 35.0 33.0 39.0 15.0 41.0 50 32.59868609159905 35.0 33.0 39.0 15.0 41.0 51 32.187182621228864 35.0 31.0 39.0 13.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 3.0 12 3.0 13 8.0 14 7.0 15 46.0 16 80.0 17 196.0 18 407.0 19 749.0 20 1295.0 21 1968.0 22 3103.0 23 4910.0 24 7551.0 25 10984.0 26 13696.0 27 14779.0 28 14972.0 29 15464.0 30 16961.0 31 19557.0 32 23954.0 33 31277.0 34 53228.0 35 76902.0 36 68383.0 37 52753.0 38 63925.0 39 100836.0 40 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.66930741410186 17.980547199876938 24.51932321511279 32.83082217090841 2 33.40283548723128 20.824561960863655 28.521242745329506 17.25135980657556 3 20.664043754587727 21.484191464778792 40.10012323116461 17.751641549468868 4 21.087076110710747 24.202968583577317 38.10702216990517 16.602933135806765 5 18.930948746202322 29.653397718969405 35.22404830260357 16.1916052322247 6 75.11215373631205 2.055469072656225 21.193921041763158 1.6384561492685554 7 71.61386007828607 7.5553913216500606 18.87777709037342 1.9529715096904507 8 70.51196947478778 4.268279428755833 22.56217154380057 2.657579552655824 9 37.426491857881196 23.90784917308043 27.82700150318612 10.83865746585226 10 28.84896741647619 23.46274556359447 37.6191137054123 10.069173314517032 11 27.59358127822654 20.9135158001749 37.96824080406245 13.524662117536113 12 16.930323394023706 31.13300772661074 37.70555944775049 14.231109431615064 13 12.53814397479864 33.75848363801138 37.96824080406245 15.73513158312753 14 11.012552189317848 24.8794859404444 48.94283712585463 15.165124744383116 15 13.016521670793878 22.71432942683296 47.95698780897665 16.312161093396515 16 13.734004611554301 24.158826076851437 37.64904366262417 24.458125648970093 17 15.262271700473026 23.815383998006897 43.55561203418369 17.366732267336385 18 19.408992029267818 26.14824859588371 37.37131372447384 17.071445650374624 19 20.431626768417374 25.471229619622015 38.00903918149091 16.0881044304697 20 15.047244186649234 30.200330065561655 38.694251274531275 16.058174473257832 21 16.378709266415076 26.103102850368607 36.40619800923983 21.111989873976487 22 12.53563587782558 30.319213862084766 36.584272894327185 20.56087736576247 23 12.221956549728038 25.003553137378503 47.72774774563884 15.04674256725462 24 13.247099385850655 26.232687860643445 43.83819095981527 16.682021793690634 25 12.575431016464822 31.679104040878634 37.84534405238244 17.900120890274103 26 12.68344639277133 32.179218577307076 37.750705193265595 17.386629836656002 27 12.85533463865847 28.867192921147105 42.73747080157107 15.540001638623355 28 11.740569137365126 25.28044704320448 42.81973638228748 20.15924743714291 29 12.572421300097147 30.632893190182305 39.59515970725492 17.199525802465626 30 18.501228131484478 29.220834594348755 36.816857086962415 15.461080187204356 31 18.419631376627546 27.814461018320813 36.86635020056415 16.899557404487485 32 16.76712988430985 29.683494882646144 36.848459108822986 16.700916124221028 33 13.267832987494629 29.240899370133246 37.1639777080341 20.327289934338022 34 13.449586414809142 27.207167137910222 39.154236259390736 20.189010187889906 35 19.39561551207816 27.678187749451144 35.47703168395303 17.449165054517668 36 14.176265711137455 30.963627577696663 37.19675017514877 17.66335653601711 37 14.692097655263744 30.74458710871597 36.342325139659195 18.220990096361085 38 14.118077861362433 32.00248134393868 35.647582278121206 18.231858516577685 39 18.481497768629726 27.647254553450058 34.44620382802481 19.425043849895413 40 13.315152417053053 26.834463927713305 36.7780651871124 23.07231846812125 41 15.024002488032199 27.232080901175966 34.46175402925778 23.282162581534052 42 15.616080580139549 26.438686225364215 36.74311903595441 21.202114158541825 43 15.051089935341261 28.664204272794002 37.03773682705668 19.246968964808055 44 12.459556936309385 33.9773569005272 33.76634234186031 19.796743821303107 45 15.428976545949173 31.951650578617972 34.238366192190455 18.3810066832424 46 14.992066053241881 29.33453499046087 33.850614400155166 21.82278455614208 47 13.678826478146952 28.578427356315306 37.93981570503442 19.802930460503326 48 15.438507314446806 26.621944510862566 38.26135373698089 19.678194437709738 49 15.502380184027434 29.567119183096096 35.35079080297561 19.579709829900864 50 13.89937180531148 29.695533748116837 34.90669043227888 21.498404014292806 51 13.718621616786193 26.87191817584435 37.51527849072757 21.894181716641892 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 862.0 1 4474.5 2 8087.0 3 29777.0 4 51467.0 5 30024.5 6 8582.0 7 8300.5 8 8019.0 9 9339.5 10 10660.0 11 12009.0 12 13358.0 13 13214.0 14 13070.0 15 11817.0 16 10564.0 17 9212.5 18 7861.0 19 7076.5 20 6292.0 21 5908.5 22 5525.0 23 5056.0 24 4587.0 25 4820.0 26 5453.0 27 5853.0 28 6055.0 29 6257.0 30 7089.5 31 7922.0 32 9557.0 33 11192.0 34 13686.5 35 16181.0 36 17551.0 37 18921.0 38 21221.5 39 23522.0 40 27196.0 41 30870.0 42 36155.0 43 41440.0 44 46300.0 45 51160.0 46 51254.0 47 51348.0 48 47313.5 49 43279.0 50 37430.0 51 31581.0 52 28630.5 53 25680.0 54 22166.5 55 18653.0 56 17262.0 57 15871.0 58 14644.0 59 13417.0 60 11012.5 61 8608.0 62 7394.5 63 6181.0 64 5170.0 65 4159.0 66 3708.5 67 3258.0 68 2780.0 69 2302.0 70 2225.0 71 2148.0 72 1991.5 73 1835.0 74 1566.5 75 976.5 76 655.0 77 469.5 78 284.0 79 200.0 80 116.0 81 83.5 82 51.0 83 32.0 84 13.0 85 11.5 86 10.0 87 8.0 88 6.0 89 3.5 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 598063.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.826222019373745 #Duplication Level Percentage of deduplicated Percentage of total 1 79.50704330217339 30.074510720519733 2 9.161656583245325 6.931017119661896 3 3.3586925513734425 3.8113995042920616 4 1.784265267436249 2.6996805659000334 5 1.0753617862100924 2.033843683816664 6 0.7134048157295372 1.6191245369685536 7 0.5158550493806713 1.3658993339381795 8 0.38478875130498086 1.1644083789935837 9 0.310365195999741 1.0565948529875384 >10 2.599956703493351 22.456883877675814 >50 0.49414722404425226 12.497498102616822 >100 0.08956139948775867 5.158510929692326 >500 0.003564632815433181 0.862617099201638 >1k 8.911582038582952E-4 1.1085253343980408 >5k 0.0 0.0 >10k+ 4.455791019291476E-4 7.159485959337079 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42478 7.102596214780048 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4745 0.7933946758117457 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1832 0.30632224364322824 No Hit CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 817 0.1366076817994091 No Hit CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT 775 0.12958501027483726 No Hit CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC 716 0.11971982884746254 No Hit AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC 634 0.10600889872806044 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1377781270535044 0.0 2 0.0 0.0 0.0 0.42587486602582003 0.0 3 0.0 0.0 0.0 0.6738420534291538 0.0 4 0.0 0.0 0.0 0.8604444682249195 0.0 5 0.0 0.0 0.0 1.8521460113733836 0.0 6 0.0 0.0 0.0 2.099109959987493 0.0 7 0.0 0.0 0.0 2.912903824513471 0.0 8 0.0 0.0 0.0 3.84390942091385 0.0 9 0.0 0.0 0.0 4.592325557675362 0.0 10 0.0 0.0 0.0 5.088259932482029 0.0 11 0.0 0.0 0.0 5.713779317563534 0.0 12 0.0 0.0 0.0 6.22242138370038 0.0 13 0.0 0.0 0.0 6.421564283361452 0.0 14 0.0 0.0 0.0 6.591278845205271 0.0 15 0.0 0.0 0.0 7.172488517096025 0.0 16 0.0 0.0 0.0 7.8553597196282 0.0 17 0.0 0.0 0.0 8.78653252249345 0.0 18 0.0 0.0 0.0 9.188831276972493 0.0 19 0.0 0.0 0.0 9.659517475583677 0.0 20 0.0 0.0 0.0 10.154448611601119 0.0 21 0.0 0.0 0.0 10.616272867574152 0.0 22 1.6720646487075776E-4 0.0 0.0 11.101004409234479 0.0 23 1.6720646487075776E-4 0.0 0.0 11.490595472383344 0.0 24 0.0033441292974151554 0.0 0.0 11.806950103918817 0.0 25 0.0038457486920274284 0.0 0.0 12.124809593638128 0.0 26 0.0038457486920274284 0.0 0.0 12.482129809066938 0.0 27 0.004347368086639702 0.0 0.0 12.800992537575473 0.0 28 0.004514574551510459 0.0 0.0 13.158312753004282 0.0 29 0.004514574551510459 0.0 0.0 13.572315960024278 0.0 30 0.004514574551510459 0.0 0.0 13.951707428816027 0.0 31 0.004514574551510459 0.0 0.0 14.266389995702793 0.0 32 0.004514574551510459 0.0 0.0 14.59210818927103 0.0 33 0.004514574551510459 0.0 0.0 14.927189944872028 0.0 34 0.004514574551510459 0.0 0.0 15.342865216540732 0.0 35 0.004514574551510459 0.0 0.0 15.698680573785705 0.0 36 0.004514574551510459 0.0 0.0 16.076567184393618 0.0 37 0.004514574551510459 0.0 0.0 16.442749342460576 0.0 38 0.004514574551510459 0.0 0.0 16.827993037522802 0.0 39 0.004514574551510459 0.0 0.0 17.212567906725546 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTGCGA 45 3.8380676E-10 45.000004 1 ATACCGG 45 3.8380676E-10 45.000004 1 TCACGAC 80 0.0 45.0 24 GGTACTC 25 3.8885533E-5 45.0 7 GTACCGG 35 1.2106284E-7 45.0 1 GCCCAAA 25 3.8885533E-5 45.0 12 CTACTTG 20 7.030569E-4 45.0 40 GATAATC 20 7.030569E-4 45.0 8 TACTTGC 20 7.030569E-4 45.0 41 GATCGGC 20 7.030569E-4 45.0 9 TTTACGG 55 1.8189894E-12 45.0 1 CTATCGG 20 7.030569E-4 45.0 1 TAGACGG 50 2.1827873E-11 45.0 1 TCCGAAT 40 6.8048394E-9 45.0 36 TGGACCG 20 7.030569E-4 45.0 31 TAGGTCC 20 7.030569E-4 45.0 32 ATGGACC 20 7.030569E-4 45.0 30 CGGCAGT 35 1.2106284E-7 45.0 24 GAATCCG 20 7.030569E-4 45.0 8 GTGATCC 25 3.8885533E-5 45.0 13 >>END_MODULE