##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043463_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2426475
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.536197982670334	31.0	31.0	33.0	30.0	34.0
2	30.575347366035093	31.0	31.0	34.0	26.0	34.0
3	31.524399798060973	31.0	31.0	34.0	28.0	34.0
4	35.155079281674034	37.0	35.0	37.0	32.0	37.0
5	35.48284404331386	37.0	35.0	37.0	33.0	37.0
6	35.780935719510815	37.0	35.0	37.0	35.0	37.0
7	33.77029847824519	37.0	35.0	37.0	32.0	37.0
8	34.77703664781215	37.0	35.0	37.0	32.0	37.0
9	36.799312995188494	39.0	37.0	39.0	32.0	39.0
10	36.80517623301291	39.0	35.0	39.0	32.0	39.0
11	36.66132970667326	39.0	35.0	39.0	33.0	39.0
12	36.68264828609403	39.0	35.0	39.0	33.0	39.0
13	36.684591846196646	39.0	35.0	39.0	33.0	39.0
14	37.95322226686861	40.0	36.0	41.0	33.0	41.0
15	38.03966247334096	40.0	36.0	41.0	33.0	41.0
16	37.9156813896702	40.0	36.0	41.0	33.0	41.0
17	37.851844342101195	40.0	36.0	41.0	33.0	41.0
18	37.60710660526072	39.0	36.0	41.0	33.0	41.0
19	37.33538404475628	38.0	36.0	41.0	33.0	41.0
20	37.00286382509608	38.0	35.0	41.0	32.0	41.0
21	36.93052967782483	38.0	35.0	41.0	32.0	41.0
22	36.841030301157026	38.0	35.0	40.0	32.0	41.0
23	36.76577751676815	38.0	35.0	40.0	32.0	41.0
24	36.61937872840231	38.0	35.0	40.0	32.0	41.0
25	36.520217599604365	38.0	35.0	40.0	32.0	41.0
26	36.25810239132898	38.0	35.0	40.0	31.0	41.0
27	36.14762031341761	37.0	35.0	40.0	31.0	41.0
28	35.97039573867441	37.0	35.0	40.0	31.0	41.0
29	35.85867482665183	37.0	35.0	40.0	30.0	41.0
30	35.65698018730875	37.0	35.0	40.0	30.0	41.0
31	35.32899494122132	37.0	34.0	40.0	28.0	41.0
32	35.149205328717585	37.0	34.0	40.0	26.0	41.0
33	34.805355505414234	37.0	34.0	40.0	24.0	41.0
34	34.611274379501126	37.0	34.0	40.0	23.0	41.0
35	34.406923211654764	37.0	34.0	40.0	22.0	41.0
36	34.257320598811035	37.0	34.0	40.0	21.0	41.0
37	34.122065959880075	36.0	34.0	40.0	21.0	41.0
38	34.00787603416479	36.0	33.0	40.0	20.0	41.0
39	33.883363727217464	36.0	33.0	40.0	20.0	41.0
40	33.77398489578504	36.0	33.0	40.0	20.0	41.0
41	33.66453064630792	35.0	33.0	40.0	18.0	41.0
42	33.54460606435261	35.0	33.0	40.0	18.0	41.0
43	33.36297015217548	35.0	33.0	40.0	18.0	41.0
44	33.14664276367982	35.0	33.0	40.0	18.0	41.0
45	32.931232755334385	35.0	33.0	40.0	18.0	41.0
46	32.91907314107914	35.0	33.0	40.0	18.0	41.0
47	32.8919828145767	35.0	33.0	39.0	18.0	41.0
48	32.779855551777786	35.0	32.0	39.0	18.0	41.0
49	32.725170051205964	35.0	32.0	39.0	18.0	41.0
50	32.640953646750944	35.0	32.0	39.0	17.0	41.0
51	32.239554909900164	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	14.0
13	34.0
14	60.0
15	119.0
16	300.0
17	640.0
18	1451.0
19	2545.0
20	4595.0
21	7259.0
22	11402.0
23	17508.0
24	27148.0
25	41759.0
26	55433.0
27	62262.0
28	64626.0
29	65366.0
30	69147.0
31	78001.0
32	92562.0
33	119967.0
34	204628.0
35	238663.0
36	255016.0
37	255819.0
38	320851.0
39	429044.0
40	249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.91625712195675	16.786737963506734	23.21161355464202	32.0853913598945
2	34.39066959272195	19.368466602787997	26.107954955233414	20.132908849256637
3	24.604910415314396	20.151371845990585	36.67323998804851	18.570477750646514
4	22.5150475483984	23.06943199497213	33.487672446656156	20.927848009973317
5	20.380758095591343	31.584829845763917	32.24657167289999	15.787840385744753
6	77.52760691950257	2.1854748142882165	18.762773158594257	1.524145107614956
7	73.5509329376977	7.24734439876776	17.553776568891085	1.6479460946434643
8	72.33052061117465	3.949515243305618	20.55174687561174	3.168217269907994
9	40.05217445059191	19.304175810589435	26.794259161953036	13.849390576865616
10	26.65001699996909	19.163519096631944	34.78131857942077	19.4051453239782
11	24.13154885172936	16.36497388186567	41.45907128653705	18.044405979867914
12	21.019462388856265	18.315828516675424	41.17244150465181	19.492267589816503
13	17.328676372103565	21.223132321577598	42.545626886738994	18.902564419579843
14	17.294758858014198	19.573661381221733	43.379717491422745	19.751862269341327
15	21.323813350642393	16.905675929074068	41.93779041613864	19.832720304144903
16	21.359379346583008	17.72377625980074	39.05142233074728	21.865422062868976
17	21.793218557784442	19.97078065918668	38.15992334559392	20.07607743743496
18	22.76425679226038	23.828310615192823	35.223070503508175	18.184362089038625
19	23.79163189400262	21.43722890200806	35.13673126654921	19.634407937440113
20	23.07272895867462	23.208440227078377	36.83145303372176	16.88737778052525
21	21.01830845156039	20.029342976952165	37.42779134340968	21.524557228077768
22	21.620910992283044	21.396264128004617	36.73856108140409	20.244263798308246
23	21.192388135051875	21.10266951029786	38.77781555548687	18.927126799163396
24	23.410131981578218	19.482830031218125	36.291657651531544	20.815380335672117
25	19.142707013259976	21.450375544771738	36.819872448716765	22.587044993251524
26	17.851080270763145	21.43924829227583	38.02004966051577	22.689621776445254
27	19.618664935760723	22.32151577906222	37.78650099424062	20.27331829093644
28	20.98195942674044	22.782142820346387	35.86140388835657	20.37449386455661
29	20.78232827455465	21.383529605703746	38.911672281807974	18.92246983793363
30	24.686304206719626	21.3753284084938	35.3547017793301	18.583665605456474
31	24.630090975592164	22.282941303743083	33.88685232693516	19.200115393729586
32	25.056553230509277	21.72047929609825	32.8811547615368	20.341812711855674
33	21.31598306184898	23.417344089677414	35.68192542680225	19.584747421671356
34	21.976406103504054	24.520178448160397	33.83822211232343	19.665193336012116
35	24.37667810300951	26.73404836233631	30.69333086061056	18.19594267404362
36	23.03221751718027	25.831422124687048	30.719995054554445	20.416365303578235
37	22.19029662370311	28.463140976107315	30.624094622858262	18.722467777331314
38	21.87032629637643	25.91928620735841	30.154771839808774	22.055615656456382
39	22.044199919636508	23.861445100402847	33.575248045003555	20.519106934957087
40	19.61742857437229	24.388093839829384	34.060767162241525	21.933710423556807
41	19.966989150928814	27.111262221947474	30.552179602097695	22.369569025026014
42	19.93031042973861	23.04058356257534	32.0504435446481	24.978662463037946
43	20.863268733450788	22.99994848494215	34.44836645751553	21.68841632409153
44	22.17529543885678	24.895702613874036	32.47698822365778	20.45201372361141
45	22.901616542515377	25.245881371124778	31.931217094756796	19.921284991603045
46	20.772272535262058	25.327934555270502	32.62329098795578	21.27650192151166
47	20.47385610814041	26.65323153957902	33.126902193511164	19.74601015876941
48	21.90135896722612	25.867152968812785	32.3544235980177	19.877064465943395
49	20.250115908880165	23.981207306895804	34.66209212952946	21.10658465469457
50	19.576340164230004	24.434004059386556	35.29885945661917	20.690796319764267
51	19.22838685747844	22.62430068308967	34.464068247148646	23.68324421228325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1099.0
1	12607.0
2	24115.0
3	97277.0
4	170439.0
5	103212.0
6	35985.0
7	32960.5
8	29936.0
9	30577.5
10	31219.0
11	31638.5
12	32058.0
13	30740.0
14	29422.0
15	27058.0
16	24694.0
17	22749.0
18	20804.0
19	18939.5
20	17075.0
21	16236.0
22	15397.0
23	14805.0
24	14213.0
25	14970.0
26	16890.5
27	18054.0
28	20224.0
29	22394.0
30	24963.5
31	27533.0
32	31276.5
33	35020.0
34	40303.5
35	45587.0
36	49829.5
37	54072.0
38	55898.5
39	57725.0
40	62730.0
41	67735.0
42	75252.0
43	82769.0
44	89305.5
45	95842.0
46	100569.0
47	105296.0
48	120862.0
49	136428.0
50	140368.0
51	144308.0
52	139071.5
53	133835.0
54	131174.0
55	128513.0
56	128267.5
57	128022.0
58	126995.5
59	125969.0
60	123778.5
61	121588.0
62	117993.0
63	114398.0
64	106896.0
65	99394.0
66	84446.0
67	69498.0
68	57763.0
69	46028.0
70	39449.5
71	32871.0
72	30891.5
73	28912.0
74	24593.5
75	16076.0
76	11877.0
77	8533.0
78	5189.0
79	4131.0
80	3073.0
81	2052.0
82	1031.0
83	795.0
84	559.0
85	439.5
86	320.0
87	206.5
88	93.0
89	59.0
90	25.0
91	31.0
92	37.0
93	27.5
94	18.0
95	10.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2426475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.75961266621876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.87110773849243	22.87654391520706
2	8.803381235827711	5.2397043146258175
3	3.098285174650321	2.7661130018124447
4	1.6116962030753863	1.9185381895655578
5	1.1015937975490668	1.6391502365284614
6	0.7818292612570249	1.3960161591675009
7	0.6215187695012157	1.2947310491598751
8	0.5417104107476365	1.2896873600886343
9	0.4252032855869927	1.1388496575125253
>10	5.5334538217754625	37.424565911508026
>50	0.5202494625105368	10.019438295159635
>100	0.08324177659588522	4.137716787002255
>500	0.004095951044555619	0.8437671040233505
>1k	0.0020479755222778096	1.3635140576367435
>5k	1.462839658769864E-4	0.27700392544116087
>10k+	4.3885189763095923E-4	6.374660035560997
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118178	4.870357205411142	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	16475	0.6789684624815834	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11778	0.4853954810991253	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6363	0.2622322504868173	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5000	0.2060602314056399	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4832	0.19913660763041038	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3344	0.13781308276409196	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3142	0.1294882494153041	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2823	0.11634160665162427	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045621735233208664	0.0
2	0.0	0.0	0.0	0.147168217269908	0.0
3	0.0	0.0	0.0	0.24859106316776394	0.0
4	0.0	0.0	0.0	0.3170854840869986	0.0
5	0.0	0.0	0.0	0.6866339030898732	0.0
6	0.0	0.0	0.0	0.8589414685912692	0.0
7	0.0	0.0	0.0	1.2722158686984206	0.0
8	0.0	0.0	0.0	1.6982660031527215	0.0
9	0.0	0.0	0.0	2.1016907241986833	0.0
10	0.0	0.0	0.0	2.3310357617531605	0.0
11	0.0	0.0	0.0	2.5682935121936143	0.0
12	0.0	0.0	0.0	2.759764679215735	0.0
13	0.0	0.0	0.0	2.8591269227995344	0.0
14	0.0	0.0	0.0	2.931206791745227	0.0
15	0.0	0.0	0.0	3.132692486013662	0.0
16	0.0	0.0	0.0	3.3576278346160584	0.0
17	0.0	0.0	0.0	3.6668830299096427	0.0
18	0.0	0.0	0.0	3.824477894888676	0.0
19	0.0	0.0	0.0	4.015372093262861	0.0
20	0.0	0.0	0.0	4.225883225666863	0.0
21	0.0	0.0	0.0	4.446532521456022	0.0
22	0.0	0.0	0.0	4.666357576319558	0.0
23	0.0	0.0	0.0	4.865988728505342	0.0
24	1.236361388433839E-4	0.0	0.0	5.035699935091027	0.0
25	1.236361388433839E-4	0.0	0.0	5.208872953564327	0.0
26	1.236361388433839E-4	0.0	0.0	5.3968823086988325	0.0
27	1.236361388433839E-4	0.0	0.0	5.589795897340792	0.0
28	1.648481851245119E-4	0.0	0.0	5.798617335847268	0.0
29	1.648481851245119E-4	0.0	0.0	6.006490897289278	0.0
30	1.648481851245119E-4	0.0	0.0	6.214158398499881	0.0
31	1.648481851245119E-4	0.0	0.0	6.430974973984895	0.0
32	1.648481851245119E-4	0.0	0.0	6.661432736788964	0.0
33	1.648481851245119E-4	0.0	0.0	6.884595967401271	0.0
34	1.648481851245119E-4	0.0	0.0	7.142624589167414	0.0
35	1.648481851245119E-4	0.0	0.0	7.377533252969843	0.0
36	1.648481851245119E-4	0.0	0.0	7.635685510874828	0.0
37	1.648481851245119E-4	0.0	0.0	7.941684954512204	0.0
38	1.648481851245119E-4	0.0	0.0	8.304021265415882	0.0
39	1.648481851245119E-4	0.0	0.0	8.67225089893776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	32385	0.0	44.152386	1
CGTTTTA	1480	0.0	43.63176	1
ACTACGG	120	0.0	43.125004	1
TACGGGT	295	0.0	40.42373	3
CACTTAC	5415	0.0	40.096954	36
ATAACGC	45	1.9297659E-8	40.0	10
CGCACTT	5490	0.0	39.67213	34
CTTACTG	5445	0.0	39.586777	38
ACTTACT	5440	0.0	39.581802	37
GCACTTA	5480	0.0	39.58029	35
CCGCACT	5545	0.0	39.197475	33
ACCCGCA	5555	0.0	39.086407	31
TTGTAGG	605	0.0	39.049587	1
CGGTCTA	365	0.0	38.835617	30
CCCGCAC	5655	0.0	38.75332	32
GCGCGAC	2160	0.0	38.750004	8
AACACGT	680	0.0	38.713234	40
TCAACGC	5860	0.0	38.70307	15
CAACGCA	5820	0.0	38.698452	16
CGCAAGC	5835	0.0	38.637535	19
>>END_MODULE