FastQCFastQC Report
Thu 26 May 2016
SRR1043462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043462_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2542242
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT955733.759398200486028No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC200730.7895786475087738No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105700.41577473741681553No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC81010.3186557377307117No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC65830.258944663804626No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA50440.1984075473538711No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39970.15722342719536533No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC37600.147900947274099No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC33190.13055405425604644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGG351.212702E-745.0000041
CCGTACC253.8921455E-545.038
CGCATCG650.044.99999620
CGTTTTT248800.043.4535751
TACGCGG2950.041.9491541
CGTTTTA12600.041.6071431
CGCACTT65700.041.2328834
TCACGAC5050.040.99009724
CACTTAC66300.040.927636
ACTTACT66150.040.78231437
GCACTTA66400.040.76430535
ACCCGCA66450.040.73363531
CTTACGG2600.040.6730771
CTTACTG66300.040.58823438
CCGCACT66600.040.5405433
CCCGCAC67250.040.34944532
GTTGATC21550.040.30162415
AACGCAA71750.039.95121817
CGGTCTA5250.039.8571430
TCAACGC71600.039.84636715