##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043462_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2542242
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46384923229181	31.0	31.0	33.0	30.0	34.0
2	30.49143747920143	31.0	31.0	34.0	26.0	34.0
3	31.467772147576824	31.0	31.0	34.0	28.0	34.0
4	35.09726100032963	37.0	35.0	37.0	32.0	37.0
5	35.41867139320332	37.0	35.0	37.0	33.0	37.0
6	35.72175032904027	37.0	35.0	37.0	35.0	37.0
7	33.69667718494148	37.0	35.0	37.0	32.0	37.0
8	34.68760173107045	37.0	35.0	37.0	31.0	37.0
9	36.649036559068726	39.0	37.0	39.0	32.0	39.0
10	36.702802880292275	39.0	35.0	39.0	32.0	39.0
11	36.62429422533339	39.0	35.0	39.0	32.0	39.0
12	36.63146506115468	39.0	35.0	39.0	32.0	39.0
13	36.66532179076579	39.0	35.0	39.0	33.0	39.0
14	37.799480143904475	40.0	36.0	41.0	33.0	41.0
15	37.93817229044284	40.0	36.0	41.0	33.0	41.0
16	37.83218120068821	40.0	36.0	41.0	33.0	41.0
17	37.71907001772451	40.0	36.0	41.0	32.0	41.0
18	37.576017546716635	39.0	36.0	41.0	33.0	41.0
19	37.36570908670378	39.0	36.0	41.0	32.0	41.0
20	36.98316604005441	39.0	35.0	41.0	32.0	41.0
21	36.95773140401268	38.0	35.0	41.0	32.0	41.0
22	36.918870036762826	38.0	35.0	40.0	32.0	41.0
23	36.8559169426042	38.0	35.0	40.0	32.0	41.0
24	36.752362678297345	38.0	35.0	40.0	32.0	41.0
25	36.64477063945919	38.0	35.0	40.0	31.0	41.0
26	36.36362431271295	38.0	35.0	40.0	31.0	41.0
27	36.234459583312685	38.0	35.0	40.0	31.0	41.0
28	36.05421159747971	38.0	35.0	40.0	30.0	41.0
29	36.01133802368146	38.0	35.0	40.0	30.0	41.0
30	35.872867335210415	38.0	35.0	40.0	30.0	41.0
31	35.6484339413793	38.0	35.0	40.0	29.0	41.0
32	35.51286659570568	38.0	35.0	40.0	28.0	41.0
33	35.12931341705471	37.0	34.0	40.0	25.0	41.0
34	35.06708606025705	37.0	34.0	40.0	25.0	41.0
35	34.952813304162234	37.0	34.0	40.0	25.0	41.0
36	34.830444937972075	37.0	34.0	40.0	24.0	41.0
37	34.68318240356347	37.0	34.0	40.0	24.0	41.0
38	34.560580385344906	37.0	34.0	40.0	23.0	41.0
39	34.43497589922596	37.0	34.0	40.0	23.0	41.0
40	34.33135476480996	36.0	34.0	40.0	23.0	41.0
41	34.218472906985255	36.0	33.0	40.0	23.0	41.0
42	34.09827506586706	36.0	33.0	40.0	23.0	41.0
43	33.91470520902416	36.0	33.0	40.0	22.0	41.0
44	33.677562560920634	35.0	33.0	40.0	21.0	41.0
45	33.42280121247308	35.0	33.0	40.0	20.0	41.0
46	33.43016597161088	35.0	33.0	40.0	20.0	41.0
47	33.39575815362975	35.0	33.0	39.0	20.0	41.0
48	33.29495697105153	35.0	33.0	39.0	20.0	41.0
49	33.21309812362474	35.0	33.0	39.0	20.0	41.0
50	33.14590074430365	35.0	33.0	39.0	20.0	41.0
51	32.701378940321185	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	10.0
12	15.0
13	38.0
14	57.0
15	128.0
16	265.0
17	672.0
18	1378.0
19	2619.0
20	4580.0
21	7151.0
22	10978.0
23	16385.0
24	24956.0
25	36576.0
26	47965.0
27	55895.0
28	60158.0
29	63452.0
30	70147.0
31	81384.0
32	98398.0
33	128156.0
34	210025.0
35	253178.0
36	275311.0
37	295707.0
38	351507.0
39	444952.0
40	191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.843955846846992	16.864798866512317	23.216200503335244	29.075044783305444
2	32.44525894859734	20.723833529616773	26.519465888770622	20.311441633015267
3	27.035781802047172	21.293606194846912	32.754749547840056	18.915862455265863
4	23.696917917334385	24.21150307484496	29.932830942136903	22.158748065683756
5	21.90837850999236	33.09857991489402	28.356073103976726	16.636968471136896
6	81.06667264564113	2.389544347076321	15.053680963496	1.4901020437865473
7	76.07143615753338	7.799100164343127	14.339154179657168	1.7903094984663144
8	74.28152001265025	4.339988089253501	17.742292039860878	3.6361998582353685
9	41.38382577268411	18.07101762932089	24.97869990347103	15.566456694523968
10	26.44524006762535	18.172424183063608	31.12374825055994	24.2585874987511
11	23.308166571081745	15.353259052442686	40.03245953768367	21.3061148387919
12	21.889261525850017	17.970869807044334	39.874921427621764	20.264947239483888
13	19.399530021138823	21.59928126433282	40.918724495937056	18.08246421859131
14	17.009592320479324	19.091652171587125	43.576181968514405	20.322573539419142
15	21.421092091154186	17.387369101761358	40.22323602552393	20.968302781560528
16	22.696777096751607	16.851936204342465	35.877308297164475	24.573978401741456
17	21.114944997368465	19.410347244676156	38.133505779544194	21.34120197841118
18	25.487620769383874	21.137366151609484	33.5086903607131	19.86632271829354
19	26.09110383669218	19.583934180931635	32.65121888474818	21.673743097627998
20	24.953879292372637	20.675805057111006	36.57149870075311	17.798816949763243
21	24.329233802289473	17.901639576405394	34.043493892398914	23.725632728906216
22	24.073396631791937	20.02814838241206	33.61281105417974	22.285643931616267
23	22.45828681927212	19.765742207075487	35.55137551814501	22.22459545550738
24	26.539920275095763	18.03573381291002	33.50105930119949	21.923286610794722
25	20.682295391233406	20.68681895743993	32.94210385950669	25.688781791819977
26	19.922336268537773	21.87569082723045	35.208528535048984	22.993444369182793
27	21.157623861143037	20.73878096577745	35.45972413326505	22.643871039814464
28	23.19543143414356	22.3107792255812	33.246166179301575	21.24762316097366
29	21.773064877379888	19.998686199032193	36.00628893708782	22.221959986500107
30	25.405685218008355	20.766551728749665	31.752877971491305	22.074885081750676
31	27.079601391212954	21.527061546461745	31.269407082409934	20.123929979915367
32	27.25318832746843	19.96658854664505	30.928251519721567	21.851971606164952
33	21.967066864602188	22.309048469815227	34.51614755794295	21.207737107639634
34	22.482674741429022	23.97175406589931	31.999392662067578	21.546178530604088
35	27.924288875724656	23.695580515151587	29.19096608426735	19.189164524856405
36	24.991129876699386	23.339359510227588	29.776118874599668	21.893391738473365
37	23.098037086949237	28.1855149903117	28.44135216080924	20.275095761929823
38	22.194228558886213	23.50999629460925	30.54571516008311	23.750059986421434
39	22.433033519232236	21.630238191328758	35.19035559950626	20.746372689932745
40	20.842429634944274	21.61328465189388	34.932551661092845	22.611734052069004
41	23.420980378736562	25.509963252908257	28.87207433438673	22.196982033968442
42	20.91657678537291	21.43206665612479	30.67465646464813	26.976700093854166
43	21.73632565271127	21.116400405626216	35.1790270163108	21.968246925351718
44	25.24326952351507	23.15700078906729	31.16194288348631	20.43778680393133
45	24.083151800654697	24.56969084768484	30.900008732449546	20.447148619210918
46	22.151785707261542	25.595871675473852	30.402023096148987	21.850319521115612
47	22.867295875058314	25.502607540902872	31.5190292662933	20.11106731774552
48	24.115996824849876	24.772543290528596	30.952403429728566	20.159056454892966
49	21.16427153669871	24.600097079664327	32.84112999470546	21.394501388931502
50	22.3559755522881	23.17855656542532	33.56136040550034	20.904107476786237
51	22.251343499163337	21.682042858233007	32.75762102899724	23.308992613606417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1087.0
1	11569.5
2	22052.0
3	78128.0
4	134204.0
5	80459.5
6	26715.0
7	24468.5
8	22222.0
9	22213.0
10	22204.0
11	21963.5
12	21723.0
13	21329.0
14	20935.0
15	19606.0
16	18277.0
17	17992.0
18	17707.0
19	16783.0
20	15859.0
21	15459.0
22	15059.0
23	14591.0
24	14123.0
25	14439.5
26	16600.5
27	18445.0
28	20246.0
29	22047.0
30	25615.0
31	29183.0
32	31288.0
33	33393.0
34	36301.0
35	39209.0
36	42356.0
37	45503.0
38	49277.5
39	53052.0
40	61331.5
41	69611.0
42	80249.5
43	90888.0
44	102594.5
45	114301.0
46	125949.5
47	137598.0
48	150632.5
49	163667.0
50	153044.0
51	142421.0
52	130857.0
53	119293.0
54	122779.5
55	126266.0
56	132525.0
57	138784.0
58	141415.5
59	144047.0
60	140138.5
61	136230.0
62	137362.5
63	138495.0
64	130048.0
65	121601.0
66	107421.5
67	93242.0
68	77431.0
69	61620.0
70	52929.0
71	44238.0
72	41313.0
73	38388.0
74	32032.0
75	19929.0
76	14182.0
77	10759.5
78	7337.0
79	5473.0
80	3609.0
81	2681.0
82	1753.0
83	1316.5
84	880.0
85	532.0
86	184.0
87	147.0
88	110.0
89	70.5
90	31.0
91	26.5
92	22.0
93	15.0
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2542242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.71532312183248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73564371274475	20.76732839869621
2	9.136343553164883	4.881607403497418
3	3.0172579738084737	2.418210651366568
4	1.521811590760421	1.626227535108582
5	0.8931635207732478	1.1930576029045423
6	0.6199091048799074	0.9936643225819598
7	0.4644930667901389	0.8686357657004633
8	0.3892081281170746	0.8318256723432976
9	0.3229655469768784	0.7765316050236006
>10	4.621814158493744	30.828675329514137
>50	1.0785536335695143	19.213615962878595
>100	0.19151807336898535	7.63977582301486
>500	0.004203920997979773	0.7789676527828896
>1k	0.002179810887841364	1.1111946321590278
>5k	4.671023331088638E-4	0.8206052146153066
>10k+	4.671023331088638E-4	5.250076427812527
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95573	3.759398200486028	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	20073	0.7895786475087738	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10570	0.41577473741681553	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8101	0.3186557377307117	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6583	0.258944663804626	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5044	0.1984075473538711	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3997	0.15722342719536533	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3760	0.147900947274099	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3319	0.13055405425604644	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05526617843619923	0.0
2	0.0	0.0	0.0	0.17909388641993956	0.0
3	0.0	0.0	0.0	0.27904503190490915	0.0
4	0.0	0.0	0.0	0.3482752625438491	0.0
5	0.0	0.0	0.0	0.745483710834767	0.0
6	0.0	0.0	0.0	0.8682886995022504	0.0
7	0.0	0.0	0.0	1.2334781661226586	0.0
8	0.0	0.0	0.0	1.6118843131377736	0.0
9	0.0	0.0	0.0	1.945644828462436	0.0
10	0.0	0.0	0.0	2.184174441300238	0.0
11	0.0	0.0	0.0	2.4391068985564712	0.0
12	0.0	0.0	0.0	2.649511730197204	0.0
13	0.0	0.0	0.0	2.7487548392324572	0.0
14	0.0	0.0	0.0	2.8291563116335894	0.0
15	0.0	0.0	0.0	3.088966353321202	0.0
16	0.0	0.0	0.0	3.364195855469306	0.0
17	0.0	0.0	0.0	3.7356396440622097	0.0
18	0.0	0.0	0.0	3.9253934125862133	0.0
19	0.0	0.0	0.0	4.160225501742163	0.0
20	0.0	0.0	0.0	4.427666602943386	0.0
21	0.0	0.0	0.0	4.695540393086103	0.0
22	0.0	0.0	0.0	4.943825174786665	0.0
23	0.0	0.0	0.0	5.178303245717756	0.0
24	1.9667679158789762E-4	0.0	0.0	5.372383903656694	0.0
25	1.9667679158789762E-4	0.0	0.0	5.577793144791094	0.0
26	1.9667679158789762E-4	0.0	0.0	5.784854470974833	0.0
27	1.9667679158789762E-4	0.0	0.0	5.989870358526057	0.0
28	3.9335358317579524E-4	0.0	0.0	6.203618695623784	0.0
29	3.9335358317579524E-4	0.0	0.0	6.4322751335238735	0.0
30	3.9335358317579524E-4	0.0	0.0	6.666005832646931	0.0
31	3.9335358317579524E-4	0.0	0.0	6.905676170875943	0.0
32	3.9335358317579524E-4	0.0	0.0	7.175477393576221	0.0
33	3.9335358317579524E-4	0.0	0.0	7.418491237262228	0.0
34	3.9335358317579524E-4	0.0	0.0	7.7163385704429395	0.0
35	3.9335358317579524E-4	0.0	0.0	7.964151327843691	0.0
36	3.9335358317579524E-4	0.0	0.0	8.259520533450395	0.0
37	3.9335358317579524E-4	0.0	0.0	8.568657114468254	0.0
38	3.9335358317579524E-4	0.0	0.0	8.957801814304068	0.0
39	3.9335358317579524E-4	0.0	0.0	9.320709830142055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGG	35	1.212702E-7	45.000004	1
CCGTACC	25	3.8921455E-5	45.0	38
CGCATCG	65	0.0	44.999996	20
CGTTTTT	24880	0.0	43.453575	1
TACGCGG	295	0.0	41.949154	1
CGTTTTA	1260	0.0	41.607143	1
CGCACTT	6570	0.0	41.23288	34
TCACGAC	505	0.0	40.990097	24
CACTTAC	6630	0.0	40.9276	36
ACTTACT	6615	0.0	40.782314	37
GCACTTA	6640	0.0	40.764305	35
ACCCGCA	6645	0.0	40.733635	31
CTTACGG	260	0.0	40.673077	1
CTTACTG	6630	0.0	40.588234	38
CCGCACT	6660	0.0	40.54054	33
CCCGCAC	6725	0.0	40.349445	32
GTTGATC	2155	0.0	40.301624	15
AACGCAA	7175	0.0	39.951218	17
CGGTCTA	525	0.0	39.85714	30
TCAACGC	7160	0.0	39.846367	15
>>END_MODULE