##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043461_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2462609
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51378436446874	31.0	31.0	33.0	30.0	34.0
2	30.512261995306602	31.0	31.0	34.0	26.0	34.0
3	31.486507602303085	31.0	31.0	34.0	28.0	34.0
4	35.1041724447527	37.0	35.0	37.0	32.0	37.0
5	35.42757498246778	37.0	35.0	37.0	33.0	37.0
6	35.7608678438193	37.0	35.0	37.0	35.0	37.0
7	33.65532287098764	37.0	35.0	37.0	31.0	37.0
8	34.69297521449812	37.0	35.0	37.0	31.0	37.0
9	36.75869169648937	39.0	37.0	39.0	32.0	39.0
10	36.709214495683234	39.0	35.0	39.0	32.0	39.0
11	36.40032826973344	38.0	35.0	39.0	32.0	39.0
12	36.362048948899314	39.0	35.0	39.0	32.0	39.0
13	36.34591646501739	39.0	35.0	39.0	32.0	39.0
14	37.515638495595525	40.0	35.0	41.0	33.0	41.0
15	37.606638325450774	40.0	35.0	41.0	33.0	41.0
16	37.38787115615999	40.0	35.0	41.0	32.0	41.0
17	37.33492811891778	40.0	35.0	41.0	32.0	41.0
18	37.129001802559806	39.0	35.0	41.0	32.0	41.0
19	36.92377677495697	38.0	35.0	40.0	32.0	41.0
20	36.63091542343913	38.0	35.0	40.0	32.0	41.0
21	36.53745803739043	38.0	35.0	40.0	31.0	41.0
22	36.47833131447177	38.0	35.0	40.0	31.0	41.0
23	36.4235625712405	37.0	35.0	40.0	31.0	41.0
24	36.305131671329065	37.0	35.0	40.0	31.0	41.0
25	36.19057592983702	37.0	35.0	40.0	31.0	41.0
26	35.913646868016805	37.0	35.0	40.0	30.0	41.0
27	35.78920039681492	36.0	35.0	40.0	30.0	41.0
28	35.62191033980628	36.0	35.0	40.0	30.0	41.0
29	35.465396252511056	36.0	34.0	40.0	29.0	41.0
30	35.265833512344024	36.0	34.0	40.0	29.0	41.0
31	35.020736950120785	36.0	34.0	40.0	27.0	41.0
32	34.876502928398295	36.0	34.0	40.0	25.0	41.0
33	34.482980854857594	36.0	33.0	40.0	23.0	41.0
34	34.3420831321578	36.0	33.0	40.0	23.0	41.0
35	34.18401581412234	36.0	33.0	40.0	22.0	41.0
36	34.084073842010646	35.0	33.0	40.0	22.0	41.0
37	33.971951292308276	35.0	33.0	40.0	21.0	41.0
38	33.84729691152757	35.0	33.0	40.0	21.0	41.0
39	33.734038168462796	35.0	33.0	40.0	21.0	41.0
40	33.6481065406648	35.0	33.0	40.0	21.0	41.0
41	33.5454077362667	35.0	33.0	40.0	20.0	41.0
42	33.41500660478379	35.0	33.0	40.0	20.0	41.0
43	33.23670667978554	35.0	33.0	40.0	19.0	41.0
44	33.01463163660979	35.0	33.0	39.0	18.0	41.0
45	32.78461582817248	35.0	32.0	39.0	18.0	41.0
46	32.79026796377338	35.0	32.0	39.0	18.0	41.0
47	32.75765377288884	35.0	32.0	39.0	20.0	41.0
48	32.66391172938944	35.0	32.0	39.0	18.0	41.0
49	32.61503429898941	35.0	32.0	39.0	18.0	41.0
50	32.540539728393746	35.0	32.0	39.0	18.0	41.0
51	32.12493457142405	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	13.0
12	16.0
13	30.0
14	67.0
15	168.0
16	383.0
17	923.0
18	1751.0
19	3127.0
20	5397.0
21	8266.0
22	12591.0
23	18795.0
24	28127.0
25	41314.0
26	54059.0
27	62831.0
28	66869.0
29	70506.0
30	77590.0
31	87480.0
32	104149.0
33	136637.0
34	248884.0
35	249887.0
36	247057.0
37	252596.0
38	304523.0
39	378369.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.453603475013693	16.638045260128585	23.025660996122404	31.882690268735313
2	33.43945384752512	19.562220393087166	26.748338855254733	20.249986904132975
3	25.24883162532095	20.738818058408786	35.28680354859419	18.72554676767607
4	23.358641180958895	23.29825806695257	32.34987771099675	20.993223041091785
5	21.376718756408344	31.803587171166843	30.88975960048875	15.929934471936063
6	78.94257675497815	2.1336720526888353	17.490474533309996	1.433276659023012
7	74.69805397446366	7.417905156685451	16.23623563464602	1.6478052342048615
8	73.7594153192813	3.6484476423175587	19.57265647936802	3.019480559033123
9	45.89498373473012	16.35817947550748	24.407366333835377	13.339470455927028
10	31.862142954890526	16.98698412943346	31.64038627325735	19.51048664241867
11	28.233917767700838	14.468638748579252	38.47699736336544	18.820446120354468
12	23.743679975180797	16.204602517086553	39.137475742190496	20.914241765542155
13	19.029736348726086	19.44937259629929	42.547030405557685	18.97386064941694
14	19.450672031166945	17.457460766203646	43.31820439217107	19.77366281045834
15	24.92608449006724	15.207732936897411	40.44101195114612	19.425170621889222
16	24.124861072139346	16.31866853406286	37.88230287471539	21.674167519082406
17	24.42243165683225	18.72603405575144	37.64519661870804	19.20633766870827
18	24.346536539093293	22.708111600339315	34.414233034964134	18.53111882560325
19	26.66663688795095	20.945428202365864	32.980550302544984	19.4073846071382
20	25.253217217999286	23.40050734810114	35.31242677989076	16.033848654008818
21	22.70855828107507	18.75458101550023	37.41304445813363	21.12381624529107
22	24.764223634365017	19.874815693437327	35.65048288217903	19.710477790018636
23	25.710374647376018	18.56786846795411	36.62509964025958	19.0966572444103
24	26.48280746151744	16.927372554879806	34.27543714816278	22.314382835439975
25	20.439338928754015	18.88294081602073	36.56467591891364	24.113044336311614
26	17.861463188025382	19.431545974208657	37.91556028585943	24.791430551906537
27	19.79230157934126	22.104686533672215	36.64105832472796	21.461953562258564
28	23.170101303130135	22.232396616758894	34.01213915810427	20.585362922006702
29	22.28027267016404	19.77561196275982	37.90881946748347	20.03529589959267
30	27.567957398027865	19.439545620112654	34.02290010310203	18.969596878757446
31	27.896024094771033	20.02989512342398	32.42402671313229	19.650054068672695
32	28.32861408368117	19.618177307075545	31.56989193168708	20.4833166775562
33	22.72861830684449	21.417853991437536	35.937170699855315	19.916357001862657
34	22.006173127768154	24.96778822785103	33.32400718100194	19.70203146337888
35	24.93091676348133	27.496488480306862	29.654281292726537	17.918313463485273
36	24.69515054968125	26.001975953145628	29.217874213892664	20.084999283280457
37	23.922189840124844	29.82747159618112	28.004689335578647	18.245649228115386
38	24.221993828496522	24.981229257263333	28.540462574448483	22.25631433979166
39	22.572726730065554	22.269227473788977	32.518398170395706	22.639647625749763
40	20.591738274326133	23.26824924297767	32.719160857448344	23.420851625247856
41	20.255225250943205	25.568533210103595	29.042897187495054	25.13334435145815
42	18.993108528394075	21.465851866861527	31.455582270673094	28.085457334071307
43	20.82681416335277	20.401005600158207	33.770525487399745	25.00165474908928
44	23.80418491120596	22.527287117037258	31.597058241888988	22.071469729867797
45	23.318318092721988	24.008602258823874	31.64152327876654	21.031556369687596
46	21.066113215699282	23.942858975988475	31.607372506151	23.383655302161245
47	20.85316832676239	24.414107152211333	33.64281540431307	21.08990911671321
48	23.322906722098395	23.448911296921274	32.534600498901774	20.69358148207856
49	20.978929257547584	21.908674905354445	34.555018681406594	22.557377155691384
50	20.101526470503437	22.49212116093135	35.963768507302625	21.442583861262587
51	19.79664656467998	20.566521116425708	34.61186895686647	25.024963362027837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1185.0
1	13648.0
2	26111.0
3	95873.5
4	165636.0
5	99645.5
6	33655.0
7	29691.5
8	25728.0
9	25288.5
10	24849.0
11	24888.5
12	24928.0
13	24000.0
14	23072.0
15	21299.0
16	19526.0
17	18082.0
18	16638.0
19	15545.0
20	14452.0
21	13854.0
22	13256.0
23	13290.5
24	13325.0
25	13668.5
26	15123.0
27	16234.0
28	16269.5
29	16305.0
30	18511.5
31	20718.0
32	22889.5
33	25061.0
34	29882.5
35	34704.0
36	37137.5
37	39571.0
38	42326.0
39	45081.0
40	51009.5
41	56938.0
42	64090.0
43	71242.0
44	83238.0
45	95234.0
46	104974.5
47	114715.0
48	125376.0
49	136037.0
50	133310.5
51	130584.0
52	123773.0
53	116962.0
54	120219.5
55	123477.0
56	132308.5
57	141140.0
58	146459.5
59	151779.0
60	153287.0
61	154795.0
62	153328.0
63	151861.0
64	140544.5
65	129228.0
66	109786.5
67	90345.0
68	73545.5
69	56746.0
70	48566.5
71	40387.0
72	37426.5
73	34466.0
74	29538.0
75	19015.5
76	13421.0
77	10181.0
78	6941.0
79	5289.5
80	3638.0
81	3062.5
82	2487.0
83	1720.0
84	953.0
85	611.0
86	269.0
87	228.0
88	187.0
89	128.5
90	70.0
91	52.0
92	34.0
93	22.5
94	11.0
95	7.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2462609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.870381033354548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.80704903913184	22.174487718072847
2	8.862292085617092	5.117154986812956
3	2.933306793353727	2.540570544355485
4	1.5742508995623592	1.8179689324986588
5	0.9953628883836197	1.4368252927047727
6	0.7812835240062365	1.3533571819885233
7	0.6187143193146397	1.2503762704584371
8	0.5203680914759029	1.201858006280705
9	0.43584268155921785	1.1324649858492264
>10	5.767669072440109	37.79466274657665
>50	0.605429679607234	11.31759566717245
>100	0.0899690796280025	4.382238615922944
>500	0.0056412306132956926	1.106535546054085
>1k	0.0022268015578798786	1.3684473410008864
>5k	1.4845343719199192E-4	0.2572830150655617
>10k+	4.4536031157597575E-4	5.7481731491859085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105805	4.296459567881056	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	16934	0.6876446890269629	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11379	0.4620709174700491	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6003	0.24376585970407808	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4763	0.19341275858246273	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4730	0.19207271637519394	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3012	0.1223093069179882	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2914	0.11832978763579602	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2714	0.11020831971295483	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05668784610143145	0.0
2	0.0	0.0	0.0	0.17213451262461885	0.0
3	0.0	0.0	0.0	0.2665059698880334	0.0
4	0.0	0.0	0.0	0.33492933713797035	0.0
5	0.0	0.0	0.0	0.7028724413822901	0.0
6	0.0	0.0	0.0	0.8628247521226472	0.0
7	0.0	0.0	0.0	1.2610609317191646	0.0
8	0.0	0.0	0.0	1.723213063868442	0.0
9	0.0	0.0	0.0	2.142280808687047	0.0
10	0.0	0.0	0.0	2.4110201822538615	0.0
11	0.0	0.0	0.0	2.6789880163680064	0.0
12	0.0	0.0	0.0	2.8930699108141	0.0
13	0.0	0.0	0.0	3.0092881167899574	0.0
14	0.0	0.0	0.0	3.0877821042642175	0.0
15	0.0	0.0	0.0	3.287326571128425	0.0
16	0.0	0.0	0.0	3.5154992124206483	0.0
17	0.0	0.0	0.0	3.8113236815101383	0.0
18	0.0	0.0	0.0	3.9890620070015177	1.6242935845682364E-4
19	0.0	0.0	0.0	4.197702517939307	1.6242935845682364E-4
20	0.0	0.0	0.0	4.431519579437905	1.6242935845682364E-4
21	0.0	0.0	0.0	4.672848998765131	1.6242935845682364E-4
22	4.060733961420591E-5	0.0	0.0	4.906909704301413	1.6242935845682364E-4
23	4.060733961420591E-5	0.0	0.0	5.127651202444237	1.6242935845682364E-4
24	2.0303669807102956E-4	0.0	0.0	5.319561489460974	1.6242935845682364E-4
25	2.4364403768523547E-4	0.0	0.0	5.507370435176677	1.6242935845682364E-4
26	2.4364403768523547E-4	0.0	0.0	5.7116253534361325	1.6242935845682364E-4
27	3.248587169136473E-4	0.0	0.0	5.915636627657903	1.6242935845682364E-4
28	3.248587169136473E-4	0.0	0.0	6.118632718389318	1.6242935845682364E-4
29	3.248587169136473E-4	0.0	0.0	6.3352322678914925	1.6242935845682364E-4
30	3.248587169136473E-4	0.0	0.0	6.555770729336245	1.6242935845682364E-4
31	3.248587169136473E-4	0.0	0.0	6.788938073401015	1.6242935845682364E-4
32	3.248587169136473E-4	0.0	0.0	7.045576459762796	1.6242935845682364E-4
33	3.248587169136473E-4	0.0	0.0	7.2942558075601935	1.6242935845682364E-4
34	3.248587169136473E-4	0.0	0.0	7.590445742706211	1.6242935845682364E-4
35	3.248587169136473E-4	0.0	0.0	7.850698182293657	1.6242935845682364E-4
36	3.248587169136473E-4	0.0	0.0	8.132797370593545	1.6242935845682364E-4
37	3.248587169136473E-4	0.0	0.0	8.528515895133982	1.6242935845682364E-4
38	3.248587169136473E-4	0.0	0.0	8.936132370181381	1.6242935845682364E-4
39	3.248587169136473E-4	0.0	0.0	9.405878074838515	1.6242935845682364E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAT	20	7.03486E-4	45.0	15
TACGTAG	20	7.03486E-4	45.0	15
CGTTTTT	28955	0.0	44.106373	1
CGTTTTA	1510	0.0	43.21192	1
CATATGC	1370	0.0	41.058395	32
TCACGAC	410	0.0	40.060974	24
ACGGGAT	360	0.0	40.0	4
GCCAGTA	1420	0.0	39.929577	24
GTTGATC	1455	0.0	39.896904	15
TAGTCAG	465	0.0	39.67742	33
CGCACTT	5155	0.0	39.544132	34
CGGTCTA	410	0.0	39.51219	30
ATAGCGG	365	0.0	39.452057	1
CACTTAC	5150	0.0	39.451454	36
CTAGCGG	755	0.0	39.33775	1
ACTTACT	5150	0.0	39.23301	37
TAACGGG	660	0.0	39.204544	2
ATTACGG	230	0.0	39.130432	1
GCACTTA	5215	0.0	39.002876	35
CCGCACT	5240	0.0	38.98855	33
>>END_MODULE