##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043460_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1231088
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50882796355744	31.0	31.0	33.0	30.0	34.0
2	30.545504464343736	31.0	31.0	34.0	26.0	34.0
3	31.45444842285848	31.0	31.0	34.0	28.0	34.0
4	35.085854951067674	37.0	35.0	37.0	32.0	37.0
5	35.4016406625684	37.0	35.0	37.0	33.0	37.0
6	35.80799829094265	37.0	35.0	37.0	35.0	37.0
7	33.73822098826404	37.0	35.0	37.0	32.0	37.0
8	34.76508746734596	37.0	35.0	37.0	31.0	37.0
9	36.722965376967366	39.0	37.0	39.0	32.0	39.0
10	36.69983624241321	39.0	35.0	39.0	32.0	39.0
11	36.551267659176276	39.0	35.0	39.0	32.0	39.0
12	36.536199686781124	39.0	35.0	39.0	32.0	39.0
13	36.60940810080189	39.0	35.0	39.0	32.0	39.0
14	37.95652138596104	40.0	36.0	41.0	33.0	41.0
15	38.08459671445096	40.0	36.0	41.0	33.0	41.0
16	38.047404409757874	40.0	36.0	41.0	33.0	41.0
17	38.006452828717364	40.0	36.0	41.0	33.0	41.0
18	37.70216751361397	39.0	36.0	41.0	33.0	41.0
19	37.38764085101958	38.0	36.0	41.0	33.0	41.0
20	37.07272672627789	38.0	35.0	41.0	33.0	41.0
21	36.98260969158988	38.0	35.0	41.0	33.0	41.0
22	36.86054124481759	38.0	35.0	41.0	32.0	41.0
23	36.743881834604835	38.0	35.0	40.0	32.0	41.0
24	36.56378747904293	38.0	35.0	40.0	32.0	41.0
25	36.4375048737377	37.0	35.0	40.0	32.0	41.0
26	36.16158389976996	37.0	35.0	40.0	31.0	41.0
27	36.07160414202721	37.0	35.0	40.0	31.0	41.0
28	35.93628156557452	36.0	35.0	40.0	31.0	41.0
29	35.844558634313714	36.0	35.0	40.0	31.0	41.0
30	35.70010186111797	36.0	35.0	40.0	30.0	41.0
31	35.21832151722704	36.0	35.0	40.0	28.0	41.0
32	35.04894207400283	36.0	34.0	40.0	26.0	41.0
33	34.88153649454791	36.0	34.0	40.0	25.0	41.0
34	34.68900842181875	36.0	34.0	40.0	24.0	41.0
35	34.44338909972317	36.0	34.0	40.0	23.0	41.0
36	34.29480508298351	36.0	34.0	40.0	22.0	41.0
37	34.20605188256241	36.0	34.0	40.0	21.0	41.0
38	34.10910999051246	36.0	34.0	40.0	21.0	41.0
39	33.99764679697958	35.0	33.0	40.0	21.0	41.0
40	33.91727804998505	35.0	33.0	40.0	21.0	41.0
41	33.83676552772832	35.0	33.0	40.0	20.0	41.0
42	33.749341233120624	35.0	33.0	40.0	20.0	41.0
43	33.58977749762811	35.0	33.0	40.0	18.0	41.0
44	33.408964265755166	35.0	33.0	40.0	18.0	41.0
45	33.21932956864172	35.0	33.0	40.0	18.0	41.0
46	33.24141409874842	35.0	33.0	40.0	18.0	41.0
47	33.22928336560831	35.0	33.0	40.0	18.0	41.0
48	33.15875631961322	35.0	33.0	40.0	18.0	41.0
49	33.065855568407784	35.0	33.0	40.0	18.0	41.0
50	32.96395870969419	35.0	33.0	40.0	18.0	41.0
51	32.58758837711033	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	5.0
12	11.0
13	12.0
14	30.0
15	65.0
16	177.0
17	359.0
18	768.0
19	1373.0
20	2385.0
21	3843.0
22	5838.0
23	9214.0
24	14829.0
25	22229.0
26	27052.0
27	28868.0
28	28393.0
29	29303.0
30	32133.0
31	37357.0
32	46074.0
33	59839.0
34	98040.0
35	141532.0
36	151394.0
37	114495.0
38	143846.0
39	231491.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.088864484098615	18.24987328281975	24.97213846613727	31.68912376694436
2	32.7165076745123	20.226742523686365	28.216260738468733	18.840489063332598
3	20.86179054624852	20.84465123533005	39.575156284522315	18.71840193389912
4	21.848397515043604	25.02631818359045	36.405927114877244	16.719357186488697
5	19.619799721872035	30.723311412344202	33.82024680607722	15.836642059706534
6	76.68282039951653	2.085147446811276	19.68713853112044	1.544893622551759
7	72.83630414722587	7.711227792001872	17.810424600028593	1.6420434607436676
8	72.03465552421923	4.5097507245623385	20.764234563248117	2.691359187970316
9	36.399916171711524	24.8571182563716	27.095138609100246	11.64782696281663
10	25.71213430721443	25.752180185331998	36.352640916002755	12.183044591450814
11	25.18585186436713	22.56841103154283	39.34828379449723	12.897453309592816
12	18.57015907879859	29.89997465656395	36.507057172192404	15.022809092445058
13	14.210032101685663	31.46281987965117	36.6725205671731	17.654627451490065
14	13.501309410862586	26.29649545767646	42.836499096733945	17.365696034727005
15	15.223688314726486	24.838029450372353	41.32677761459782	18.61150462030334
16	16.387536877948612	26.14995841077161	36.55750035740743	20.905004353872346
17	17.090492312491065	26.076608658357486	40.20086297648909	16.63203605266236
18	19.702572033843236	29.390669066711723	34.97004275892544	15.936716140519605
19	20.825968574139296	28.05477756261128	36.01253525336938	15.10671860988004
20	16.217930805921267	30.151053377175312	36.35239722911766	17.27861858778576
21	17.119572280779277	28.2417666324422	34.641390379891604	19.997270706886916
22	14.217667624085362	30.500337912480667	35.78298220760823	19.49901225582574
23	13.510163367687769	27.917094472531613	44.23956695215939	14.333175207621226
24	14.934919355886825	30.114175428564	39.30758808468607	15.643317130863107
25	15.391344891673056	32.49515875388275	35.86551083269433	16.247985521749868
26	13.762622980648013	31.856780343890932	36.30187281494093	18.078723860520125
27	14.32310281637056	30.049029801281467	38.709824155543714	16.918043226804258
28	13.561824987328283	28.08296401232081	38.52047944582353	19.834731554527377
29	13.676357823323759	32.7245493417205	37.88518773637628	15.713905098579467
30	17.056457377539218	32.94443614103947	35.12583990746397	14.873266573957345
31	16.5541374783931	30.532342123390045	34.879391237669445	18.03412916054742
32	15.737136581625357	33.516612947246664	33.5439058783775	17.202344592750478
33	14.144642787518032	31.624140597585225	33.937541426770466	20.293675188126276
34	15.117522061785998	32.19388053494145	34.71839543558218	17.970201967690368
35	17.69776003015219	33.05287680490753	32.73502787777966	16.514335287160627
36	15.308897495548655	34.47373380294504	33.842665999506124	16.37470270200018
37	14.889024992526936	33.12825728136413	35.61126418257671	16.371453543532226
38	15.40685962335755	34.6943516629193	33.19917016492728	16.69961854879586
39	17.71595531757275	31.105412448175922	33.705470283196654	17.473161951054678
40	14.643957215081294	31.559401115111186	34.75421740769141	19.04242426211611
41	15.06764747930286	32.494752609074254	32.37664569876402	20.06095421285887
42	15.117359603862598	31.255604798357222	34.3110321926621	19.316003405118074
43	15.247569629465968	31.64785945440131	34.122662230482305	18.981908685650417
44	13.814284600288525	36.66634716608399	31.688555164212467	17.830813069415022
45	15.299150020144783	33.81935328749854	33.129069570981116	17.752427121375565
46	14.464522438688379	34.517597442262456	32.64925009422559	18.36863002482357
47	13.774726095941151	32.572976099190306	35.025847055612594	18.626450749255945
48	15.737380268510456	30.656784892712786	35.3341921942217	18.27164264455506
49	15.24448292892141	33.5741230521295	33.41475182927622	17.766642189672872
50	14.04863015478991	31.61244362710058	34.58842909686391	19.750497121245598
51	14.150816188607152	30.62819229819477	35.08668754792509	20.134303965272995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1067.0
1	7521.5
2	13976.0
3	57971.0
4	101966.0
5	59667.0
6	17368.0
7	17674.5
8	17981.0
9	21999.5
10	26018.0
11	29053.5
12	32089.0
13	31900.5
14	31712.0
15	28108.0
16	24504.0
17	20700.0
18	16896.0
19	14670.0
20	12444.0
21	11760.5
22	11077.0
23	10729.0
24	10381.0
25	10704.5
26	11256.5
27	11485.0
28	13531.5
29	15578.0
30	17840.0
31	20102.0
32	23475.0
33	26848.0
34	33153.0
35	39458.0
36	43439.5
37	47421.0
38	49001.5
39	50582.0
40	53317.0
41	56052.0
42	63553.0
43	71054.0
44	73815.5
45	76577.0
46	79033.5
47	81490.0
48	81124.0
49	80758.0
50	77920.0
51	75082.0
52	66375.5
53	57669.0
54	52147.0
55	46625.0
56	43056.0
57	39487.0
58	36762.0
59	34037.0
60	28558.0
61	23079.0
62	19490.0
63	15901.0
64	13070.0
65	10239.0
66	8426.5
67	6614.0
68	5323.5
69	4033.0
70	3889.0
71	3745.0
72	3668.5
73	3592.0
74	2992.5
75	1933.0
76	1473.0
77	1057.0
78	641.0
79	488.5
80	336.0
81	234.5
82	133.0
83	93.0
84	53.0
85	36.0
86	19.0
87	12.5
88	6.0
89	8.0
90	10.0
91	7.5
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1231088.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.50177698967972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.27279458442464	26.22277708526405
2	8.993946416510322	6.026263742061158
3	3.3681725980420394	3.3851930172706366
4	1.8762824874523414	2.5143518985707947
5	1.2122942750887484	2.030700622494434
6	0.8516368351157507	1.711880839574671
7	0.629563953353293	1.4764057816188203
8	0.4972791123712788	1.332778713943077
9	0.3891621348114683	1.1733860747953335
>10	3.1206010058531883	22.830387487139962
>50	0.6358700055731503	14.591147401307298
>100	0.14689129582350066	7.328946882059022
>500	0.0037536106229351094	0.9103651771246162
>1k	0.00125120354097837	0.7972716488125149
>5k	2.5024070819567397E-4	0.7032925196517548
>10k+	2.5024070819567397E-4	6.964851108311894
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83078	6.748339680022875	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8389	0.6814297596922397	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3421	0.27788427797200527	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1826	0.14832408406222788	No Hit
GCGAAGGGTCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA	1575	0.12793561467579895	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1457	0.1183505971953264	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07107534148655498	0.0
2	0.0	0.0	0.0	0.2345892413864809	0.0
3	0.0	0.0	0.0	0.40183967352455713	0.0
4	0.0	0.0	0.0	0.5129608931286797	0.0
5	0.0	0.0	0.0	1.1184415736324291	0.0
6	0.0	0.0	0.0	1.3396280363385884	0.0
7	0.0	0.0	0.0	1.8538073638927517	0.0
8	0.0	0.0	0.0	2.4284210389509115	0.0
9	0.0	0.0	0.0	2.8833844534265625	0.0
10	0.0	0.0	0.0	3.195547353235512	0.0
11	0.0	0.0	0.0	3.5252557087714282	0.0
12	0.0	0.0	0.0	3.800134515160573	0.0
13	0.0	0.0	0.0	3.926445550602394	0.0
14	0.0	0.0	0.0	4.033586551083269	0.0
15	0.0	0.0	0.0	4.359477145419336	0.0
16	0.0	0.0	0.0	4.758717492169528	0.0
17	0.0	0.0	0.0	5.229926698984963	0.0
18	0.0	0.0	0.0	5.468658617418089	0.0
19	0.0	0.0	0.0	5.745893063696503	0.0
20	0.0	0.0	0.0	6.068209583717817	0.0
21	0.0	0.0	0.0	6.392231911934809	0.0
22	0.0	0.0	0.0	6.753457104609906	0.0
23	0.0	0.0	0.0	7.051892309891738	0.0
24	4.061448084945999E-4	0.0	0.0	7.307601081319938	0.0
25	4.061448084945999E-4	0.0	0.0	7.549094784450827	0.0
26	4.061448084945999E-4	0.0	0.0	7.819505997946532	0.0
27	4.873737701935199E-4	0.0	0.0	8.076676890685313	0.0
28	4.873737701935199E-4	0.0	0.0	8.353586421116932	0.0
29	4.873737701935199E-4	0.0	0.0	8.673384843325579	0.0
30	4.873737701935199E-4	0.0	0.0	8.961016596701453	0.0
31	4.873737701935199E-4	0.0	0.0	9.22159910583159	0.0
32	4.873737701935199E-4	0.0	0.0	9.496721599105832	0.0
33	4.873737701935199E-4	0.0	0.0	9.799705626242803	0.0
34	4.873737701935199E-4	0.0	0.0	10.148665245701363	0.0
35	4.873737701935199E-4	0.0	0.0	10.451161899068142	0.0
36	4.873737701935199E-4	0.0	0.0	10.752277660086039	0.0
37	4.873737701935199E-4	0.0	0.0	11.072157311256385	0.0
38	4.873737701935199E-4	0.0	0.0	11.399022653132839	0.0
39	4.873737701935199E-4	0.0	0.0	11.727431345281571	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAGT	20	7.0334814E-4	45.000004	33
GACGTGT	40	6.8157533E-9	45.000004	43
CCATCGG	20	7.0334814E-4	45.000004	1
CTTGCGG	310	0.0	45.000004	1
CATACGG	60	0.0	45.000004	1
ATGCGAT	20	7.0334814E-4	45.000004	32
CGAGAAT	40	6.8157533E-9	45.000004	5
GAACACG	20	7.0334814E-4	45.000004	8
TCGACTA	20	7.0334814E-4	45.000004	27
CTTACGT	20	7.0334814E-4	45.000004	31
CGGACTG	20	7.0334814E-4	45.000004	5
TCTGCGA	25	3.8909686E-5	45.0	1
CGTGACG	25	3.8909686E-5	45.0	11
TGCGGAT	25	3.8909686E-5	45.0	33
AGCGCAA	25	3.8909686E-5	45.0	13
TCGTCGA	25	3.8909686E-5	45.0	31
AGCGATA	25	3.8909686E-5	45.0	7
TCCCGTA	55	1.8189894E-12	45.0	29
CCGAATC	25	3.8909686E-5	45.0	21
CATCCGG	55	1.8189894E-12	45.0	1
>>END_MODULE