##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043459_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4296778
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49379441991185	31.0	31.0	33.0	30.0	34.0
2	30.524161127244646	31.0	31.0	34.0	26.0	34.0
3	31.523492486695844	31.0	31.0	34.0	28.0	34.0
4	35.14506311473388	37.0	35.0	37.0	32.0	37.0
5	35.46890460712655	37.0	35.0	37.0	33.0	37.0
6	35.720255503076956	37.0	35.0	37.0	35.0	37.0
7	33.65148653246688	37.0	35.0	37.0	31.0	37.0
8	34.674095566491914	37.0	35.0	37.0	31.0	37.0
9	36.69209207457309	39.0	37.0	39.0	32.0	39.0
10	36.66925798819488	39.0	35.0	39.0	32.0	39.0
11	36.47540249926806	39.0	35.0	39.0	32.0	39.0
12	36.48339639609028	39.0	35.0	39.0	32.0	39.0
13	36.509978639808715	39.0	35.0	39.0	32.0	39.0
14	37.74256966499084	40.0	36.0	41.0	33.0	41.0
15	37.802007224948554	40.0	36.0	41.0	33.0	41.0
16	37.58683646211184	40.0	36.0	41.0	32.0	41.0
17	37.56562056499079	40.0	36.0	41.0	32.0	41.0
18	37.41964211322996	39.0	36.0	41.0	32.0	41.0
19	37.25479626827357	39.0	35.0	41.0	32.0	41.0
20	37.05421574025933	39.0	35.0	41.0	32.0	41.0
21	36.93914626261817	38.0	35.0	41.0	32.0	41.0
22	36.870125242681844	38.0	35.0	41.0	32.0	41.0
23	36.82467281297754	38.0	35.0	40.0	32.0	41.0
24	36.69681398480443	38.0	35.0	40.0	31.0	41.0
25	36.57906226479469	38.0	35.0	40.0	31.0	41.0
26	36.301832210088584	38.0	35.0	40.0	31.0	41.0
27	36.17620575231022	38.0	35.0	40.0	30.0	41.0
28	35.9928869957908	38.0	35.0	40.0	30.0	41.0
29	35.899757911625876	38.0	35.0	40.0	30.0	41.0
30	35.76259280791328	37.0	34.0	40.0	30.0	41.0
31	35.6105805326689	38.0	34.0	40.0	29.0	41.0
32	35.54726215783082	38.0	34.0	40.0	28.0	41.0
33	35.28227476495179	37.0	34.0	40.0	26.0	41.0
34	35.19588957120894	37.0	34.0	40.0	26.0	41.0
35	35.07773080201025	37.0	34.0	40.0	26.0	41.0
36	34.96251959026042	37.0	34.0	40.0	25.0	41.0
37	34.85022381887079	37.0	34.0	40.0	25.0	41.0
38	34.7276317277737	37.0	34.0	40.0	24.0	41.0
39	34.57841643203349	36.0	34.0	40.0	24.0	41.0
40	34.48400778443755	36.0	34.0	40.0	24.0	41.0
41	34.39182382706298	36.0	33.0	40.0	23.0	41.0
42	34.25918839651479	35.0	33.0	40.0	23.0	41.0
43	34.08256814757476	35.0	33.0	40.0	23.0	41.0
44	33.855799624742076	35.0	33.0	40.0	23.0	41.0
45	33.62143983235811	35.0	33.0	40.0	22.0	41.0
46	33.60460535778204	35.0	33.0	39.0	22.0	41.0
47	33.55609621907392	35.0	33.0	39.0	23.0	41.0
48	33.44856285337525	35.0	33.0	39.0	22.0	41.0
49	33.414629985538	35.0	33.0	39.0	22.0	41.0
50	33.34989170955539	35.0	33.0	39.0	22.0	41.0
51	32.9212163160396	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	5.0
11	17.0
12	37.0
13	62.0
14	115.0
15	215.0
16	524.0
17	1190.0
18	2342.0
19	4354.0
20	7280.0
21	11800.0
22	17308.0
23	25910.0
24	38783.0
25	56237.0
26	74035.0
27	89610.0
28	98421.0
29	106506.0
30	119955.0
31	140257.0
32	170336.0
33	225897.0
34	403874.0
35	425711.0
36	430334.0
37	498579.0
38	610901.0
39	735784.0
40	392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.89882698151964	17.262074047111582	24.025839826958713	27.81325914441007
2	30.475858887752636	21.262047981068605	27.283746099984686	20.978347031194073
3	27.5677728753964	21.86678017807762	31.06620821462035	19.499238731905628
4	24.533243281361056	24.68721912093201	27.890409976964136	22.889127620742798
5	22.5906016089265	34.74161336703921	25.94320674700904	16.724578277025252
6	84.16257484096222	2.237839609121067	12.194300008052545	1.4052855418641597
7	79.39893566760955	7.517190788074227	11.554960484344317	1.5289130599719138
8	78.85280552078791	3.6543661320179908	14.508941350937842	2.9838869962562646
9	45.93809594072582	18.25423608108215	20.713055224170297	15.094612754021735
10	30.306243422396967	18.18720911343337	27.775928847150123	23.730618617019545
11	26.473092163476913	14.570056912412044	38.11681683345055	20.840034090660488
12	21.961572136144806	16.99627022852938	38.25189479186498	22.790262843460845
13	18.534329676795032	21.580519170410945	40.04984665253825	19.835304500255774
14	19.46525978302812	18.739064480408345	40.37185537628428	21.42382036027926
15	24.324994216596714	14.28395881751396	39.510814847776636	21.880232118112687
16	23.727895646458812	15.665878013711671	35.03909208248599	25.567134257343525
17	24.129894539582917	19.722452498127666	34.14914617417982	21.9985067881096
18	25.504668847215285	23.034934548631554	30.201606878456367	21.25878972569679
19	26.953033179745383	20.399448144633027	29.823928534357602	22.82359014126399
20	27.333574133920813	21.93392351198968	32.14541221352371	18.587090140565792
21	23.71402478787594	17.923779166622058	33.70688455396113	24.65531149154087
22	25.307963315768234	20.237140480611284	31.829338169204924	22.625558034415555
23	26.502090636286074	19.661569669180025	32.78163312137606	21.05470657315784
24	27.980873110037336	17.151875195786236	31.04689141491601	23.820360279260413
25	22.228679256875733	19.558608799430644	31.71813856801538	26.494573375678243
26	20.628387131008395	19.16319623680814	34.30475114143668	25.903665490746786
27	22.230913489130693	21.417140936767037	33.61688688594105	22.73505868816122
28	23.98066644355375	22.71846485901762	31.068628632896555	22.232240064532075
29	23.07056589844763	19.461117144055383	35.41206922954828	22.056247727948712
30	29.146653608820376	20.072342578555375	30.06578417595696	20.71521963666729
31	28.70134319250378	20.636532769437938	29.37359109546735	21.288532942590937
32	29.115909642062025	18.82024158567187	28.144414256449835	23.91943451581627
33	24.049601817920312	21.979329627921203	31.39654876281716	22.574519791341327
34	24.265763788587634	22.065766488284943	30.15540947193455	23.513060251192872
35	27.224655311491542	25.186663122926063	26.858124855414918	20.73055671016748
36	25.466151614069894	24.21661067897853	27.107939949422565	23.20929775752901
37	26.047145093369963	27.778093259647115	25.333703533205576	20.841058113777347
38	25.16571719553582	23.277744393589803	25.728487718006377	25.828050692868004
39	23.989975744616082	20.683684379318642	31.466531433553236	23.85980844251204
40	21.96320126383071	21.988662202236185	31.29135366081282	24.75678287312028
41	21.92265925770426	25.686479496962605	26.72872557064852	25.66213567468461
42	21.270030706729553	20.565805354616877	29.1086018407281	29.05556209792547
43	22.16495709110408	20.02023376585898	31.88179608069116	25.933013062345783
44	25.451233459117507	21.804896599265774	29.78324688871522	22.9606230529015
45	26.190135957687367	22.71755720216404	29.23627890479797	21.856027935350628
46	23.963816608630932	22.821588641535588	28.6519108038628	24.56268394597068
47	21.945211039527756	25.1766323510314	30.50578829066803	22.37236831877281
48	24.454067675825932	24.276190205777446	29.619310097007574	21.650432021389047
49	22.834435477001605	21.560597266137556	31.316768052712984	24.288199204147855
50	21.10099707269028	22.63486733547789	33.820225294395016	22.443910297436823
51	21.345994603398175	20.37235807854164	31.72954246181674	26.552104856243446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1839.0
1	16870.5
2	31902.0
3	108098.0
4	184294.0
5	112207.0
6	40120.0
7	35954.5
8	31789.0
9	31360.5
10	30932.0
11	30393.5
12	29855.0
13	28347.5
14	26840.0
15	24911.0
16	22982.0
17	21753.0
18	20524.0
19	19240.5
20	17957.0
21	17928.0
22	17899.0
23	18023.0
24	18147.0
25	18346.0
26	21140.0
27	23735.0
28	26770.0
29	29805.0
30	33612.0
31	37419.0
32	43810.5
33	50202.0
34	54173.5
35	58145.0
36	64931.0
37	71717.0
38	73908.0
39	76099.0
40	87817.0
41	99535.0
42	114473.0
43	129411.0
44	142660.0
45	155909.0
46	168041.0
47	180173.0
48	211883.5
49	243594.0
50	246037.0
51	248480.0
52	236521.0
53	224562.0
54	231674.5
55	238787.0
56	253500.0
57	268213.0
58	278022.0
59	287831.0
60	292958.5
61	298086.0
62	288211.0
63	278336.0
64	258661.0
65	238986.0
66	204397.0
67	169808.0
68	142499.0
69	115190.0
70	101167.5
71	87145.0
72	82115.0
73	77085.0
74	65958.5
75	41772.5
76	28713.0
77	21920.5
78	15128.0
79	11985.0
80	8842.0
81	6453.0
82	4064.0
83	3003.0
84	1942.0
85	1417.0
86	892.0
87	584.5
88	277.0
89	197.0
90	117.0
91	90.0
92	63.0
93	39.0
94	15.0
95	13.5
96	12.0
97	7.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4296778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.155279029733393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67043822181584	17.208102311659996
2	9.299937279100504	4.120854107549825
3	2.8597278756915854	1.9007420710516139
4	1.4546667341488702	1.289141895613569
5	0.8522315071761002	0.9440713419708369
6	0.5842719100782174	0.7766824318210925
7	0.4237596163065397	0.6571958778562903
8	0.3499422589044982	0.620245471225949
9	0.276510356205097	0.5513547686701408
>10	4.158267180683374	23.229888959041997
>50	1.5445102046144021	24.0494145346593
>100	0.5137480082527185	16.215406945363064
>500	0.00712544681508606	1.0438468083787988
>1k	0.0038454792335378624	1.473769262980593
>5k	4.5240932159268973E-4	0.7209977727027681
>10k+	5.655116519908621E-4	5.198285439454229
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118887	2.7668871884933317	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	44592	1.0378008824286478	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	17131	0.39869409124697625	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13928	0.3241498629903616	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12911	0.3004809650393853	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8292	0.19298181102211936	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8065	0.18769878266924658	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	7253	0.16880090151271487	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5163	0.12015980346203596	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03225672818097654	0.0
2	0.0	0.0	0.0	0.10631221813181878	0.0
3	0.0	0.0	0.0	0.16435571025545187	0.0
4	0.0	0.0	0.0	0.20329186194865081	0.0
5	0.0	2.3273252655827228E-5	0.0	0.4393059171313947	0.0
6	0.0	2.3273252655827228E-5	0.0	0.520227016615706	0.0
7	0.0	2.3273252655827228E-5	0.0	0.7421840271943303	0.0
8	0.0	2.3273252655827228E-5	0.0	0.99735196931282	0.0
9	0.0	2.3273252655827228E-5	0.0	1.2301775888817155	0.0
10	0.0	2.3273252655827228E-5	0.0	1.3916706890605006	0.0
11	0.0	2.3273252655827228E-5	0.0	1.5506502779524565	0.0
12	0.0	2.3273252655827228E-5	0.0	1.6861937014199941	0.0
13	0.0	2.3273252655827228E-5	0.0	1.7559669128821642	0.0
14	0.0	2.3273252655827228E-5	0.0	1.805050202733304	0.0
15	0.0	2.3273252655827228E-5	0.0	1.9509036771273731	0.0
16	0.0	2.3273252655827228E-5	0.0	2.1145379165504945	0.0
17	0.0	2.3273252655827228E-5	0.0	2.3378680490358126	0.0
18	0.0	2.3273252655827228E-5	0.0	2.4667553222437837	0.0
19	0.0	2.3273252655827228E-5	0.0	2.61435429058704	0.0
20	0.0	2.3273252655827228E-5	0.0	2.7787332740951474	0.0
21	0.0	2.3273252655827228E-5	0.0	2.9515604483173203	0.0
22	2.3273252655827228E-5	2.3273252655827228E-5	0.0	3.130508487987976	0.0
23	2.3273252655827228E-5	2.3273252655827228E-5	0.0	3.291536123113645	0.0
24	6.981975796748168E-5	2.3273252655827228E-5	0.0	3.4273122791077406	0.0
25	6.981975796748168E-5	2.3273252655827228E-5	0.0	3.5612498481420265	0.0
26	6.981975796748168E-5	2.3273252655827228E-5	0.0	3.711618333551326	0.0
27	6.981975796748168E-5	2.3273252655827228E-5	0.0	3.8778126307665883	0.0
28	6.981975796748168E-5	2.3273252655827228E-5	0.0	4.042191614274696	0.0
29	6.981975796748168E-5	2.3273252655827228E-5	0.0	4.215763532581856	0.0
30	6.981975796748168E-5	2.3273252655827228E-5	0.0	4.386216835033134	0.0
31	9.309301062330891E-5	2.3273252655827228E-5	0.0	4.567212920937503	0.0
32	9.309301062330891E-5	2.3273252655827228E-5	0.0	4.776230003039487	0.0
33	9.309301062330891E-5	2.3273252655827228E-5	0.0	4.976286882868978	0.0
34	9.309301062330891E-5	2.3273252655827228E-5	0.0	5.209275415206464	0.0
35	9.309301062330891E-5	2.3273252655827228E-5	0.0	5.415034241936633	0.0
36	9.309301062330891E-5	2.3273252655827228E-5	0.0	5.637270531547126	0.0
37	9.309301062330891E-5	2.3273252655827228E-5	0.0	5.913012029013368	0.0
38	9.309301062330891E-5	2.3273252655827228E-5	0.0	6.194990758191371	0.0
39	9.309301062330891E-5	2.3273252655827228E-5	0.0	6.55607527314653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAT	20	7.035447E-4	45.0	14
CGTTTTT	33415	0.0	43.88224	1
TATACGG	245	0.0	42.2449	1
TTCCGTA	615	0.0	41.341465	45
ACTTACT	13515	0.0	40.738068	37
CACTTAC	13595	0.0	40.531445	36
CGTTTTA	1815	0.0	40.413223	1
CTTACTG	13650	0.0	40.25275	38
CGCACTT	13705	0.0	40.222546	34
ACCCGCA	13735	0.0	39.987263	31
CCGCACT	13770	0.0	39.95098	33
GCACTTA	13870	0.0	39.67916	35
CCCGCAC	13940	0.0	39.673603	32
TTATGAC	14355	0.0	39.373043	26
CTTATGA	14400	0.0	39.25	25
GCGCGAC	5370	0.0	39.217876	8
CAACGCA	14705	0.0	39.185654	16
CGGTCTA	1005	0.0	39.179104	30
AGGGTAC	2125	0.0	39.176468	5
TAGCATA	4665	0.0	39.163986	29
>>END_MODULE