FastQCFastQC Report
Thu 26 May 2016
SRR1043458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043458_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences964321
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT644086.679103742426018No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72730.7542094385583225No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.29264114335371727No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.1497426686756796No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC11530.11956599514062226TruSeq Adapter, Index 14 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT10790.11189220187053896Illumina PCR Primer Index 3 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTT351.211647E-745.0000041
CGCGATA351.211647E-745.00000410
TCAGGTT351.211647E-745.00000433
GCCGCAA351.211647E-745.00000431
CCGTTGG351.211647E-745.0000041
ATTTACG207.032722E-445.010
CCGGGTA406.8139343E-945.04
CGAACTC253.8903367E-545.033
AGATCCG207.032722E-445.09
GCGACTA207.032722E-445.09
CGTATCG253.8903367E-545.010
ATTAGCG207.032722E-445.011
GGTCGTA253.8903367E-545.07
GCGAACT253.8903367E-545.032
CGGCCCA207.032722E-445.045
GGATCGT207.032722E-445.07
TCGATGG850.045.01
ACTTACG207.032722E-445.09
TGGCACG253.8903367E-545.010
CTAACGG502.1827873E-1145.01