##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043458_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	964321
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.614052789475704	31.0	31.0	34.0	30.0	34.0
2	30.60510452432333	31.0	31.0	34.0	26.0	34.0
3	31.525242113362665	31.0	31.0	34.0	28.0	34.0
4	35.13821746078329	37.0	35.0	37.0	32.0	37.0
5	35.4735103767314	37.0	35.0	37.0	33.0	37.0
6	35.9032718358306	37.0	35.0	37.0	35.0	37.0
7	33.7183323810225	37.0	35.0	37.0	32.0	37.0
8	34.79922142108281	37.0	35.0	37.0	31.0	37.0
9	36.868536514293474	39.0	37.0	39.0	32.0	39.0
10	36.80557304051244	39.0	35.0	39.0	32.0	39.0
11	36.527038195787505	39.0	35.0	39.0	33.0	39.0
12	36.419809378827175	39.0	35.0	39.0	32.0	39.0
13	36.46670869969647	39.0	35.0	39.0	32.0	39.0
14	37.83501655569048	40.0	36.0	41.0	33.0	41.0
15	38.004181180333106	40.0	36.0	41.0	33.0	41.0
16	37.98519165298692	40.0	36.0	41.0	33.0	41.0
17	37.95430567207393	40.0	36.0	41.0	33.0	41.0
18	37.632741587085626	39.0	36.0	41.0	33.0	41.0
19	37.27164398576822	38.0	35.0	41.0	33.0	41.0
20	36.92824277393109	38.0	35.0	40.0	33.0	41.0
21	36.82066034028088	38.0	35.0	40.0	33.0	41.0
22	36.748595125482076	38.0	35.0	40.0	33.0	41.0
23	36.645014471322305	37.0	35.0	40.0	33.0	41.0
24	36.485874516888046	37.0	35.0	40.0	32.0	41.0
25	36.382174607832866	37.0	35.0	40.0	32.0	41.0
26	36.1190526805908	36.0	35.0	40.0	32.0	41.0
27	36.03080405798484	36.0	35.0	40.0	31.0	41.0
28	35.96555089021187	36.0	35.0	40.0	31.0	41.0
29	35.8735058139354	36.0	35.0	40.0	31.0	41.0
30	35.671261955303265	36.0	35.0	40.0	31.0	41.0
31	35.18961839470467	35.0	35.0	40.0	29.0	41.0
32	34.980535527070344	35.0	34.0	40.0	27.0	41.0
33	34.800843287660435	36.0	34.0	40.0	25.0	41.0
34	34.58359923718347	36.0	34.0	40.0	24.0	41.0
35	34.32129239122657	35.0	34.0	40.0	22.0	41.0
36	34.144007026705836	35.0	34.0	40.0	21.0	41.0
37	34.05130864100232	35.0	34.0	40.0	21.0	41.0
38	33.944387812771886	35.0	34.0	40.0	21.0	41.0
39	33.796668329321875	35.0	33.0	40.0	20.0	41.0
40	33.74556397714039	35.0	33.0	40.0	20.0	41.0
41	33.64036353040118	35.0	33.0	40.0	18.0	41.0
42	33.54581099032376	35.0	33.0	40.0	18.0	41.0
43	33.35472524190596	35.0	33.0	40.0	18.0	41.0
44	33.17327321503939	35.0	33.0	40.0	18.0	41.0
45	32.95723519450473	35.0	33.0	40.0	15.0	41.0
46	32.95881039612328	35.0	33.0	40.0	17.0	41.0
47	32.929629241715155	35.0	33.0	40.0	17.0	41.0
48	32.87650896330164	35.0	33.0	40.0	15.0	41.0
49	32.780281669692975	35.0	33.0	40.0	15.0	41.0
50	32.644484564787035	35.0	33.0	39.0	15.0	41.0
51	32.199568400978514	35.0	32.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	12.0
14	20.0
15	32.0
16	96.0
17	226.0
18	471.0
19	874.0
20	1573.0
21	2585.0
22	4438.0
23	7130.0
24	11617.0
25	17629.0
26	22004.0
27	23840.0
28	23123.0
29	23665.0
30	25253.0
31	29827.0
32	36432.0
33	48552.0
34	84627.0
35	123696.0
36	110063.0
37	86556.0
38	108659.0
39	171204.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.982470567373312	18.75516555171981	25.35493886371862	31.907425017188263
2	33.1824153990217	20.01657124546702	29.099335179883045	17.701678175628242
3	20.057740109361923	21.042992945295186	41.074393277757096	17.824873667585795
4	21.35378157273356	23.3656635083131	38.26682193999716	17.013732978956178
5	18.90407862112305	30.747748934224184	35.2496730860367	15.098499358616063
6	75.55305753996853	1.911811523341294	21.175106629431486	1.3600243072586826
7	71.71284250783711	7.817728743851891	18.899101025488402	1.570327722822587
8	70.53481154097028	4.198498217917063	22.530982940327963	2.7357073007846973
9	39.4247351245073	22.041311969769403	27.934163001739048	10.599789903984254
10	29.990013698757984	22.606994973665408	37.54071517679279	9.862276150783817
11	28.17526529029234	21.1864099195185	38.253029852092816	12.385294938096338
12	18.385890175574314	29.93142325014181	38.09965768660021	13.583028887683666
13	13.842278660321613	32.7680305624372	37.72467881545668	15.665011961784508
14	12.328156288206936	25.103362884350748	47.230642078726895	15.337838748715418
15	14.371562996139254	22.025342183774903	47.224420084183585	16.37867473590226
16	15.530202080012776	23.63103157558531	37.94006352656429	22.89870281783763
17	16.793266972304867	24.303214386080985	42.3267770794165	16.57674156219765
18	20.638252200252822	26.032825169212327	37.724367715729514	15.604554914805341
19	21.79160258876453	24.794129755548205	37.517797496891596	15.896470158795669
20	16.925173256623054	28.84589260215219	38.543182197629214	15.685751943595546
21	17.274330850411847	26.745139844512355	36.908871630919585	19.07165767415622
22	14.118742617862722	28.934970824030586	36.35034392074838	20.59594263735831
23	13.370340374211493	25.5227253165699	46.83616762468099	14.270766684537616
24	14.046463781251264	26.44389160870706	43.315659412166696	16.193985197874984
25	13.60656876703919	31.191999344616573	38.785943684727386	16.415488203616846
26	12.907942479734446	32.22402083953372	38.3217828917964	16.546253788935427
27	13.57089599832421	28.988168877375898	42.67469027429663	14.766244850003266
28	12.658025698911462	26.234002992779377	41.68197104491139	19.426000263397768
29	14.055278273520955	30.59997656382055	40.13632390044394	15.208421262214552
30	18.82734068842222	29.620012423249104	36.56707673067371	14.985570157654971
31	18.98475715036798	28.032366815614303	37.478391531450626	15.504484502567092
32	18.641095651759112	30.425449616880684	35.91366360371702	15.019791127643181
33	14.479410901556639	29.08336539388855	36.953981091358585	19.48324261319623
34	15.870337781713765	27.879098350030745	38.20906109065342	18.041502777602066
35	20.602475731628783	28.11999323876593	35.46132460041832	15.81620642918696
36	16.578193360924423	31.329401724114685	35.53878843248254	16.553616482478343
37	16.212028982050583	30.38231045471373	36.621933982563895	16.78372658067179
38	15.980881884766587	31.66404133063575	35.212963318231175	17.14211346636649
39	19.093227255239697	28.741259393915513	33.94326163175955	18.222251719085243
40	14.56568922589055	27.51905226579116	37.13908542902208	20.77617307929621
41	16.131350452805655	28.32262286105975	34.73044764139742	20.815579044737177
42	16.271137930212035	28.16385830029627	35.94809197352334	19.616911795968356
43	15.812680632279086	29.240367056198092	35.979616745876115	18.96733556564671
44	14.311728148614414	34.299159719636926	33.250027739725674	18.13908439202299
45	16.85787201564624	31.313950437665465	34.32964749289915	17.498530053789143
46	16.66758268252999	29.78489527864684	34.52678102001305	19.020741018810128
47	15.330061255536279	28.099149557045838	37.78161006552797	18.78917912188991
48	16.355964455819173	26.925681386177423	37.17040280155675	19.54795135644666
49	17.045361451218007	29.779399183466914	35.50767845976599	17.667560905549085
50	15.019272628097905	29.41313110468402	35.05482095692202	20.512775310296053
51	15.038871910909336	27.461809915992703	36.9162343244625	20.583083848635464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1321.0
1	7052.5
2	12784.0
3	46601.0
4	80418.0
5	47526.0
6	14634.0
7	14487.0
8	14340.0
9	16176.0
10	18012.0
11	19736.5
12	21461.0
13	21279.0
14	21097.0
15	19043.0
16	16989.0
17	14840.5
18	12692.0
19	11227.0
20	9762.0
21	9363.0
22	8964.0
23	8149.5
24	7335.0
25	7452.0
26	8162.0
27	8755.0
28	9531.5
29	10308.0
30	12328.0
31	14348.0
32	16284.5
33	18221.0
34	21500.0
35	24779.0
36	27990.5
37	31202.0
38	32895.0
39	34588.0
40	40694.5
41	46801.0
42	56215.0
43	65629.0
44	68930.0
45	72231.0
46	73813.0
47	75395.0
48	74265.0
49	73135.0
50	69645.0
51	66155.0
52	57431.5
53	48708.0
54	41867.0
55	35026.0
56	30389.5
57	25753.0
58	22631.5
59	19510.0
60	17133.0
61	14756.0
62	12434.0
63	10112.0
64	8264.5
65	6417.0
66	5446.5
67	4476.0
68	3657.5
69	2839.0
70	2634.5
71	2430.0
72	2296.5
73	2163.0
74	1808.0
75	1157.0
76	861.0
77	665.5
78	470.0
79	340.5
80	211.0
81	144.0
82	77.0
83	65.0
84	53.0
85	52.0
86	51.0
87	37.5
88	24.0
89	14.5
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	964321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.35836859721693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69083623967664	27.03688756746046
2	9.646462433042352	6.628734238673505
3	3.411993970173687	3.516916394361274
4	1.759336604590777	2.4179174218842405
5	1.107871693613506	1.9032332003797912
6	0.7298647805604169	1.5046177893972996
7	0.5717940943200298	1.3752138578051585
8	0.4070405365137201	1.1188199030037866
9	0.32580365772924025	1.0074673946324468
>10	2.501315681524515	18.505198818235623
>50	0.6298844683818559	15.223661801150303
>100	0.21215508430820895	10.471293123168818
>500	0.003760503710041311	0.8724552268518598
>1k	0.001253501236680437	0.6996438435250383
>5k	3.1337530917010923E-4	0.7830885924834724
>10k+	3.1337530917010923E-4	6.934850826986867
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64408	6.679103742426018	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7273	0.7542094385583225	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2822	0.29264114335371727	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1444	0.1497426686756796	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1153	0.11956599514062226	TruSeq Adapter, Index 14 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	1079	0.11189220187053896	Illumina PCR Primer Index 3 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1172845971414083	0.0
2	0.0	0.0	0.0	0.38514146223093765	0.0
3	0.0	0.0	0.0	0.6174292585145403	0.0
4	0.0	0.0	0.0	0.7825195137303864	0.0
5	0.0	0.0	0.0	1.7021303072317207	0.0
6	0.0	0.0	0.0	1.9335885042428818	0.0
7	0.0	0.0	0.0	2.6416514832716493	0.0
8	0.0	0.0	0.0	3.488050140980026	0.0
9	0.0	0.0	0.0	4.09894630522409	0.0
10	0.0	0.0	0.0	4.553359306703888	0.0
11	0.0	0.0	0.0	5.076214248160104	0.0
12	0.0	0.0	0.0	5.511857566100915	0.0
13	0.0	0.0	0.0	5.700591400581342	0.0
14	0.0	0.0	0.0	5.844215774622766	0.0
15	0.0	0.0	0.0	6.36302641962583	0.0
16	0.0	0.0	0.0	6.97703358114155	0.0
17	0.0	0.0	0.0	7.725643224610892	0.0
18	0.0	0.0	0.0	8.115036383113091	0.0
19	0.0	0.0	0.0	8.540102310330274	0.0
20	0.0	0.0	0.0	9.02251428725497	0.0
21	2.0739981811035952E-4	0.0	0.0	9.490719376639106	0.0
22	3.110997271655393E-4	0.0	0.0	9.969190757019707	0.0
23	0.001762898453938056	0.0	0.0	10.349562023434105	0.0
24	0.003007297362600213	0.0	0.0	10.679638833956743	0.0
25	0.003007297362600213	0.0	0.0	10.985760965487634	0.0
26	0.003007297362600213	0.0	0.0	11.338133256457134	0.0
27	0.003007297362600213	0.0	0.0	11.663439871163233	0.0
28	0.003007297362600213	0.0	0.0	12.031263448581955	0.0
29	0.003007297362600213	0.0	0.0	12.458714473707406	0.0
30	0.003007297362600213	0.0	0.0	12.861692320295836	0.0
31	0.003007297362600213	0.0	0.0	13.212405412720454	0.0
32	0.003007297362600213	0.0	0.0	13.577843892230906	0.0
33	0.003007297362600213	0.0	0.0	13.94068987401498	0.0
34	0.003007297362600213	0.0	0.0	14.368451998867597	0.0
35	0.003007297362600213	0.0	0.0	14.74529746837412	0.0
36	0.003007297362600213	0.0	0.0	15.106380551704255	0.0
37	0.003007297362600213	0.0	0.0	15.497329208842284	0.0
38	0.003007297362600213	0.0	0.0	15.8850631687996	0.0
39	0.003007297362600213	0.0	0.0	16.303492301837252	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTT	35	1.211647E-7	45.000004	1
CGCGATA	35	1.211647E-7	45.000004	10
TCAGGTT	35	1.211647E-7	45.000004	33
GCCGCAA	35	1.211647E-7	45.000004	31
CCGTTGG	35	1.211647E-7	45.000004	1
ATTTACG	20	7.032722E-4	45.0	10
CCGGGTA	40	6.8139343E-9	45.0	4
CGAACTC	25	3.8903367E-5	45.0	33
AGATCCG	20	7.032722E-4	45.0	9
GCGACTA	20	7.032722E-4	45.0	9
CGTATCG	25	3.8903367E-5	45.0	10
ATTAGCG	20	7.032722E-4	45.0	11
GGTCGTA	25	3.8903367E-5	45.0	7
GCGAACT	25	3.8903367E-5	45.0	32
CGGCCCA	20	7.032722E-4	45.0	45
GGATCGT	20	7.032722E-4	45.0	7
TCGATGG	85	0.0	45.0	1
ACTTACG	20	7.032722E-4	45.0	9
TGGCACG	25	3.8903367E-5	45.0	10
CTAACGG	50	2.1827873E-11	45.0	1
>>END_MODULE