FastQCFastQC Report
Thu 26 May 2016
SRR1043457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043457_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3749530
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1447913.8615773176904837No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC325210.8673353727000451No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151560.40421066106952075No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC120660.32180033230831595No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC91070.2428837747664374No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62140.1657274378388758No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC54570.14553824079284605No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC53210.1419111195269807No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA40380.10769349758503065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGA351.2129203E-745.042
CGTTTTT395000.043.9689871
ACGTAGG2800.041.7857131
CGCACTT94400.040.87658734
CACTTAC95100.040.76498436
CGTTTTA21750.040.758621
TAGCATA34300.040.7361529
ACCCGCA95300.040.53777331
GCACTTA95350.040.51651835
CCGCACT95350.040.46932633
CCCGCAC96300.040.42056332
TAGTAGG7050.040.212771
CCAGTAG35150.040.19914625
ACTTACT95900.040.09645537
TCAACGC101450.040.03203615
CTTACTG96300.039.9299138
TACGGGA7450.039.8657723
CTTATGA99500.039.8442225
ATCAACG102100.039.79921714
CAACGCA102000.039.7516