##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043457_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3749530
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.507496406216244	31.0	31.0	33.0	30.0	34.0
2	30.558059276762688	31.0	31.0	34.0	26.0	34.0
3	31.51975074209301	31.0	31.0	34.0	28.0	34.0
4	35.13735428173664	37.0	35.0	37.0	32.0	37.0
5	35.45467245228068	37.0	35.0	37.0	33.0	37.0
6	35.74990438801663	37.0	35.0	37.0	35.0	37.0
7	33.74969422834329	37.0	35.0	37.0	32.0	37.0
8	34.74572999815977	37.0	35.0	37.0	31.0	37.0
9	36.72001210818423	39.0	37.0	39.0	32.0	39.0
10	36.70913794528914	39.0	35.0	39.0	32.0	39.0
11	36.545898285918504	39.0	35.0	39.0	32.0	39.0
12	36.54912562374484	39.0	35.0	39.0	32.0	39.0
13	36.57455467751958	39.0	35.0	39.0	33.0	39.0
14	37.81597213517428	40.0	36.0	41.0	33.0	41.0
15	37.864218448712236	40.0	36.0	41.0	33.0	41.0
16	37.67978920024643	40.0	36.0	41.0	32.0	41.0
17	37.64729819470707	40.0	36.0	41.0	32.0	41.0
18	37.451654207327316	39.0	36.0	41.0	32.0	41.0
19	37.251242955783795	39.0	35.0	41.0	32.0	41.0
20	37.00681472077834	38.0	35.0	41.0	32.0	41.0
21	36.90461551181081	38.0	35.0	41.0	32.0	41.0
22	36.831144703469505	38.0	35.0	40.0	32.0	41.0
23	36.77915338722453	38.0	35.0	40.0	32.0	41.0
24	36.63842988321203	38.0	35.0	40.0	32.0	41.0
25	36.533159889372804	38.0	35.0	40.0	31.0	41.0
26	36.263772259456516	38.0	35.0	40.0	31.0	41.0
27	36.14534248292453	38.0	35.0	40.0	31.0	41.0
28	35.97166338181052	37.0	35.0	40.0	30.0	41.0
29	35.847907871119844	37.0	35.0	40.0	30.0	41.0
30	35.687028240872856	37.0	34.0	40.0	30.0	41.0
31	35.46325139417473	37.0	34.0	40.0	29.0	41.0
32	35.33814958141421	37.0	34.0	40.0	27.0	41.0
33	35.06364851061333	37.0	34.0	40.0	25.0	41.0
34	34.898306721109044	37.0	34.0	40.0	24.0	41.0
35	34.753150928249674	37.0	34.0	40.0	23.0	41.0
36	34.645400623544816	37.0	34.0	40.0	23.0	41.0
37	34.534137078513844	37.0	34.0	40.0	23.0	41.0
38	34.4109389710177	37.0	34.0	40.0	23.0	41.0
39	34.28358647617168	36.0	34.0	40.0	23.0	41.0
40	34.17006664835326	36.0	33.0	40.0	22.0	41.0
41	34.073292652679136	36.0	33.0	40.0	22.0	41.0
42	33.951779823071156	36.0	33.0	40.0	22.0	41.0
43	33.779651849698496	35.0	33.0	40.0	21.0	41.0
44	33.55562430491288	35.0	33.0	40.0	20.0	41.0
45	33.346677316890386	35.0	33.0	40.0	20.0	41.0
46	33.329197259389844	35.0	33.0	40.0	20.0	41.0
47	33.284271362010706	35.0	33.0	40.0	20.0	41.0
48	33.189717377911364	35.0	33.0	39.0	20.0	41.0
49	33.15043405440149	35.0	33.0	39.0	20.0	41.0
50	33.06719562185127	35.0	33.0	39.0	20.0	41.0
51	32.635814088699114	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	10.0
12	28.0
13	54.0
14	116.0
15	230.0
16	505.0
17	1087.0
18	2206.0
19	4157.0
20	7191.0
21	11127.0
22	16376.0
23	25253.0
24	38146.0
25	56602.0
26	74781.0
27	87389.0
28	92734.0
29	98321.0
30	107133.0
31	122928.0
32	145842.0
33	190708.0
34	335494.0
35	363187.0
36	371553.0
37	403493.0
38	516016.0
39	676541.0
40	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.064549423527748	17.18783420855414	23.588423082359654	30.159193285558455
2	32.65494608657619	20.009574533341514	27.29182057484538	20.043658805236923
3	26.054225462924684	21.33624747635037	33.90920995431427	18.70031710641067
4	22.92063271930082	23.98348593023659	31.567263096974823	21.52861825348777
5	21.274266374718962	33.13543297426611	29.602723541350517	15.98757710966441
6	80.71510295957093	2.1776596000032002	15.697914138572033	1.4093233018538325
7	76.61248209775626	7.292300635012921	14.605990617490724	1.4892266497401008
8	75.80651441647353	3.582262310209546	17.598739041959927	3.012484231356997
9	42.22222518555659	18.508239699375657	24.304352812219133	14.965182302848623
10	28.939307059818166	19.355919275215825	31.26367838102376	20.441095283942254
11	26.120980496222195	16.18781553954762	39.08740562150456	18.60379834272562
12	21.332326984982117	18.063437284139614	39.499350585273355	21.104885145604914
13	18.180091904852073	21.795878416761568	41.184335103332955	18.839694575053407
14	18.930665976802427	19.567412449026943	41.627670668057064	19.874250906113566
15	23.14652769813817	16.143569994105928	40.59255426680144	20.117348040954468
16	22.40830184049734	17.513234992119013	36.92404114649036	23.154422020893286
17	22.875267033468194	20.25307171832203	37.06317858504933	19.808482663160447
18	23.731587692324105	23.805383608078877	33.451712614647704	19.011316084949314
19	25.106373332124292	21.36654460692407	33.33300440321854	20.194077657733104
20	24.671465490341458	23.29147919872624	34.8278850949319	17.209170216000405
21	21.67140948332191	19.152480444215676	36.13754257200236	23.038567500460058
22	22.700365112427423	21.14889066096284	35.07500940117828	21.075734825431454
23	24.005808728027244	20.11172066899051	36.69099860515851	19.19147199782373
24	24.97507687630183	18.5397103103589	34.391750432720904	22.09346238061837
25	20.65226841764168	20.171754859942446	35.07418263088974	24.101794091526138
26	18.87844609857769	20.502222945275808	36.75730024829779	23.862030707848717
27	20.358631615162434	22.323997941075284	36.62136321085576	20.696007232906524
28	22.6471584438583	22.564108034873705	34.40524545743067	20.383488063837333
29	21.393427976306363	20.915821449621685	38.15928929759196	19.531461276479988
30	26.488973284651678	20.969348158302505	33.91729096713455	18.62438758991127
31	26.656247583030407	21.735124135558323	32.01131875195025	19.597309529461025
32	26.470544308219964	20.560070195464498	31.365904526700678	21.603480969614857
33	22.401154278002842	22.355441882049217	34.173083026405976	21.070320813541965
34	21.955045032310714	23.83650750894112	33.25878176731484	20.949665691433324
35	24.920216667155618	26.452142001797558	29.922470282942125	18.705171048104695
36	23.585836091456798	25.410358098214974	29.863582902390434	21.140222907937794
37	23.49803842081541	28.30706781916667	28.53082919725939	19.664064562758533
38	23.368982245774802	24.7843062997229	28.144594122463353	23.70211733203895
39	22.72135440975269	22.47161644259414	32.81499281243249	21.99203633522068
40	20.57788576168213	23.27931767448187	32.95685059194086	23.185945971895144
41	20.19895826943644	26.585652068392573	28.836387493899235	24.379002168271757
42	19.926604134384842	21.98011484106008	31.181108032206705	26.912172992348378
43	21.030635839691907	21.488559899507408	33.252354295071655	24.228449965729038
44	23.261235408171157	23.63733587943022	31.239035292423317	21.862393419975305
45	24.30032564081365	23.416401522324133	31.222526556661766	21.06074628020045
46	21.868287492032334	24.500164020557243	30.754467893309297	22.877080594101127
47	20.247364336330154	25.912100983323246	32.32661160198745	21.513923078359156
48	22.230039498283784	24.860635866361918	32.28265942664814	20.626665208706157
49	21.380252991708293	22.610780551162414	33.62338746456223	22.38557899256707
50	19.375095011908158	23.655978215936397	35.42518662339013	21.543740148765313
51	19.29652516448728	21.357423463740787	34.61175133950122	24.734300032270713
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2082.0
1	18272.0
2	34462.0
3	126513.5
4	218565.0
5	132822.5
6	47080.0
7	41727.0
8	36374.0
9	36092.0
10	35810.0
11	35942.5
12	36075.0
13	34486.5
14	32898.0
15	30818.0
16	28738.0
17	27028.0
18	25318.0
19	23008.5
20	20699.0
21	20150.5
22	19602.0
23	19737.0
24	19872.0
25	20176.0
26	23520.0
27	26560.0
28	29404.5
29	32249.0
30	36636.5
31	41024.0
32	47498.0
33	53972.0
34	62399.5
35	70827.0
36	76122.0
37	81417.0
38	86735.0
39	92053.0
40	98288.5
41	104524.0
42	117629.5
43	130735.0
44	140823.5
45	150912.0
46	161231.0
47	171550.0
48	194812.0
49	218074.0
50	219815.0
51	221556.0
52	207474.5
53	193393.0
54	191784.0
55	190175.0
56	198913.5
57	207652.0
58	212952.5
59	218253.0
60	222674.0
61	227095.0
62	216050.0
63	205005.0
64	185555.0
65	166105.0
66	139962.5
67	113820.0
68	94890.5
69	75961.0
70	65557.0
71	55153.0
72	53026.0
73	50899.0
74	43336.5
75	26855.5
76	17937.0
77	13783.5
78	9630.0
79	7253.0
80	4876.0
81	3601.5
82	2327.0
83	1607.5
84	888.0
85	813.0
86	738.0
87	444.0
88	150.0
89	120.0
90	90.0
91	80.0
92	70.0
93	45.5
94	21.0
95	14.5
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3749530.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.975453644536746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.81173560564618	19.433833956197656
2	8.871395980719104	4.431342781575591
3	2.9402459129658687	2.20301926508453
4	1.4442177620327503	1.4427997507306223
5	0.8800115492508374	1.0989343827485627
6	0.6508843620924004	0.975367932803557
7	0.4722486280692763	0.8256236603328199
8	0.38946921134701423	0.778173618717731
9	0.3131822011036933	0.703968079136335
>10	4.711099225076723	28.85564752327469
>50	1.260917839079967	21.443208481084117
>100	0.2442441291664648	9.005131049732212
>500	0.006822589087713716	1.1605757208046568
>1k	0.0026153258169566655	1.0925020164349584
>5k	4.5483927251420273E-4	0.741199670564246
>10k+	4.5483927251420273E-4	5.808672110777711
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	144791	3.8615773176904837	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	32521	0.8673353727000451	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15156	0.40421066106952075	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12066	0.32180033230831595	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9107	0.2428837747664374	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6214	0.1657274378388758	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5457	0.14553824079284605	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5321	0.1419111195269807	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4038	0.10769349758503065	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.039551623803516706	0.0
2	0.0	0.0	0.0	0.12428224337450294	0.0
3	0.0	0.0	0.0	0.19599789840326654	0.0
4	0.0	0.0	0.0	0.24525740559483455	0.0
5	0.0	0.0	0.0	0.5286795945091785	0.0
6	0.0	0.0	0.0	0.6392267830901472	0.0
7	0.0	0.0	0.0	0.9151546993889901	0.0
8	0.0	0.0	0.0	1.2291673889794188	0.0
9	0.0	0.0	0.0	1.5181636098390998	0.0
10	0.0	0.0	0.0	1.7120545775070475	0.0
11	0.0	0.0	0.0	1.9012782935461245	0.0
12	0.0	0.0	0.0	2.0520171861539978	0.0
13	0.0	0.0	0.0	2.1321072241054213	0.0
14	0.0	0.0	0.0	2.1899278042848036	0.0
15	0.0	0.0	0.0	2.353148261248743	0.0
16	0.0	0.0	0.0	2.5432520875949787	0.0
17	0.0	0.0	0.0	2.788589503217737	0.0
18	0.0	0.0	0.0	2.9325275434521125	0.0
19	0.0	0.0	0.0	3.0980949612351414	0.0
20	0.0	0.0	0.0	3.2893722679909216	0.0
21	4.26720148925332E-4	0.0	0.0	3.4876904572039695	0.0
22	4.26720148925332E-4	0.0	0.0	3.688648977338493	0.0
23	4.26720148925332E-4	0.0	0.0	3.8677380898405933	0.0
24	5.067301768488317E-4	0.0	0.0	4.0208239432675565	0.0
25	5.067301768488317E-4	0.0	0.0	4.174656556955139	0.0
26	5.067301768488317E-4	0.0	0.0	4.338757124226237	0.0
27	5.067301768488317E-4	0.0	0.0	4.520886617789429	0.0
28	5.33400186156665E-4	0.0	0.0	4.697948809584134	0.0
29	5.33400186156665E-4	0.0	0.0	4.894266748099095	0.0
30	5.33400186156665E-4	0.0	0.0	5.079569972769921	0.0
31	5.33400186156665E-4	0.0	0.0	5.272554160121402	0.0
32	5.33400186156665E-4	0.0	0.0	5.50090277981507	0.0
33	5.33400186156665E-4	0.0	0.0	5.719676866167227	0.0
34	5.33400186156665E-4	0.0	0.0	5.975202225345576	0.0
35	5.33400186156665E-4	0.0	0.0	6.19178937093449	0.0
36	5.33400186156665E-4	0.0	0.0	6.431472744583988	0.0
37	5.600701954644982E-4	0.0	0.0	6.731963739455345	0.0
38	6.134102140801647E-4	0.0	0.0	7.029067643144607	0.0
39	6.134102140801647E-4	0.0	0.0	7.391379719591522	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCGA	35	1.2129203E-7	45.0	42
CGTTTTT	39500	0.0	43.968987	1
ACGTAGG	280	0.0	41.785713	1
CGCACTT	9440	0.0	40.876587	34
CACTTAC	9510	0.0	40.764984	36
CGTTTTA	2175	0.0	40.75862	1
TAGCATA	3430	0.0	40.73615	29
ACCCGCA	9530	0.0	40.537773	31
GCACTTA	9535	0.0	40.516518	35
CCGCACT	9535	0.0	40.469326	33
CCCGCAC	9630	0.0	40.420563	32
TAGTAGG	705	0.0	40.21277	1
CCAGTAG	3515	0.0	40.199146	25
ACTTACT	9590	0.0	40.096455	37
TCAACGC	10145	0.0	40.032036	15
CTTACTG	9630	0.0	39.92991	38
TACGGGA	745	0.0	39.865772	3
CTTATGA	9950	0.0	39.84422	25
ATCAACG	10210	0.0	39.799217	14
CAACGCA	10200	0.0	39.75	16
>>END_MODULE