##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043456_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1124666
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462387055356878	31.0	31.0	33.0	30.0	34.0
2	30.475295776701707	31.0	31.0	34.0	26.0	34.0
3	31.387358558007445	31.0	31.0	34.0	28.0	34.0
4	35.013819213882165	37.0	35.0	37.0	32.0	37.0
5	35.32891276165546	37.0	35.0	37.0	33.0	37.0
6	35.787869465245684	37.0	35.0	37.0	35.0	37.0
7	33.66982019550693	37.0	35.0	37.0	31.0	37.0
8	34.71466906619388	37.0	35.0	37.0	31.0	37.0
9	36.66369837800734	39.0	37.0	39.0	32.0	39.0
10	36.63811122591063	39.0	35.0	39.0	32.0	39.0
11	36.4256508154421	39.0	35.0	39.0	32.0	39.0
12	36.36695605628693	39.0	35.0	39.0	32.0	39.0
13	36.416137768902054	39.0	35.0	39.0	32.0	39.0
14	37.75927697645346	40.0	36.0	41.0	33.0	41.0
15	37.91138169020847	40.0	36.0	41.0	33.0	41.0
16	37.86695427798119	40.0	36.0	41.0	33.0	41.0
17	37.81998566685576	40.0	36.0	41.0	33.0	41.0
18	37.51729313413938	39.0	36.0	41.0	33.0	41.0
19	37.19676864064531	38.0	35.0	41.0	33.0	41.0
20	36.8779246460727	38.0	35.0	41.0	32.0	41.0
21	36.76636708142684	38.0	35.0	40.0	32.0	41.0
22	36.68145031502686	38.0	35.0	40.0	32.0	41.0
23	36.56190815762191	37.0	35.0	40.0	32.0	41.0
24	36.36991337872755	37.0	35.0	40.0	32.0	41.0
25	36.248257704954185	37.0	35.0	40.0	31.0	41.0
26	35.96334200553765	36.0	35.0	40.0	31.0	41.0
27	35.867902114939014	36.0	35.0	40.0	31.0	41.0
28	35.75959796063898	36.0	35.0	40.0	31.0	41.0
29	35.68258843069854	36.0	35.0	40.0	30.0	41.0
30	35.52060167196306	36.0	35.0	40.0	30.0	41.0
31	35.029889762827366	35.0	34.0	40.0	27.0	41.0
32	34.87071094885059	35.0	34.0	40.0	25.0	41.0
33	34.72452087997681	36.0	34.0	40.0	25.0	41.0
34	34.518409020989345	36.0	34.0	40.0	23.0	41.0
35	34.29391214813998	35.0	34.0	40.0	22.0	41.0
36	34.13472444263452	35.0	34.0	40.0	21.0	41.0
37	34.03569682021151	35.0	33.0	40.0	21.0	41.0
38	33.92645105302374	35.0	33.0	40.0	21.0	41.0
39	33.82217653952373	35.0	33.0	40.0	21.0	41.0
40	33.732933155265656	35.0	33.0	40.0	20.0	41.0
41	33.65407685481734	35.0	33.0	40.0	20.0	41.0
42	33.568911125614186	35.0	33.0	40.0	19.0	41.0
43	33.400411322116966	35.0	33.0	40.0	18.0	41.0
44	33.20399389685471	35.0	33.0	40.0	18.0	41.0
45	33.03480588903728	35.0	33.0	40.0	18.0	41.0
46	33.04312124666345	35.0	33.0	40.0	18.0	41.0
47	33.01414642213777	35.0	33.0	40.0	18.0	41.0
48	32.959344374240885	35.0	33.0	40.0	18.0	41.0
49	32.86403518911392	35.0	33.0	40.0	17.0	41.0
50	32.757993039711344	35.0	33.0	39.0	17.0	41.0
51	32.386141307730476	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	6.0
12	3.0
13	14.0
14	25.0
15	79.0
16	166.0
17	375.0
18	739.0
19	1413.0
20	2395.0
21	3662.0
22	5655.0
23	8707.0
24	13669.0
25	20211.0
26	25509.0
27	27688.0
28	27845.0
29	28541.0
30	31080.0
31	36069.0
32	44297.0
33	57499.0
34	94145.0
35	134806.0
36	135726.0
37	100954.0
38	127398.0
39	195908.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.325563322799837	17.996542973647287	25.48285446523679	31.195039238316085
2	33.213682995662715	19.800634143825814	28.202150682958315	18.78353217755316
3	20.856147514017493	20.867350840160544	40.44409629169905	17.832405354122912
4	21.537505357146035	24.51403349972347	37.23985609949976	16.70860504363073
5	18.75605735391663	30.12618857509696	35.4111353948639	15.706618676122512
6	76.0712958336075	2.1525501793421333	20.22902799586722	1.54712599118316
7	72.37464278283508	7.546062564352439	18.38083484341129	1.698459809401191
8	71.39106187970474	4.317459583556363	21.483000286307224	2.808478250431684
9	37.835855267252676	23.079563176978766	28.161427481581196	10.923154074187359
10	27.686797680377996	25.4701395792173	36.2250659306852	10.617996809719509
11	26.03315117554901	22.534512468590677	38.672014624786385	12.760321731073937
12	19.543846795404146	29.638488226726867	35.94498277710893	14.872682200760048
13	13.381750670865838	32.27002505632783	37.68105375284751	16.667170519958816
14	12.742360843130315	26.302119918269067	44.656813667346576	16.298705571254043
15	15.551817161717347	23.927637182950313	44.258295351686634	16.262250303645704
16	16.557982547707496	25.330098002429164	37.39279039288109	20.71912905698225
17	17.78723638840331	25.368776152208746	40.505981331346376	16.338006128041567
18	20.457451367783857	28.476098681741956	36.294953346148986	14.771496604325197
19	20.807688682684457	27.402357677746103	36.58383911312336	15.206114526446074
20	16.491029336709744	30.154641466888837	36.76069161866723	16.59363757773419
21	16.775202593481087	27.071859556526118	36.71774553511887	19.435192314873927
22	14.406232605947011	30.339763094109717	36.502214879795424	18.751789420147848
23	14.18430004997039	27.424586499458503	44.14750690427202	14.243606546299079
24	14.879884338994867	28.323075473073782	40.93570891269052	15.861331275240827
25	14.799771665543371	31.831139200438173	37.017834628236294	16.351254505782162
26	14.13050630142638	32.29572157422737	36.83715876535789	16.736613358988357
27	14.328342814666756	29.761013492005628	40.42738021777132	15.4832634755563
28	14.003713102378839	27.761753267192212	39.88028445778569	18.354249172643257
29	14.394762533943412	31.51077742191904	38.165108574456774	15.929351469680778
30	18.549951718999242	31.16276298918968	35.31759651309811	14.96968877871297
31	17.517823069249	29.888695843921663	35.52885923465278	17.064621852176558
32	17.16945297537224	30.44379397972376	35.51961204482042	16.86714100008358
33	14.205372972953748	30.02260226591717	36.14833203813399	19.62369272299509
34	14.30860362098614	30.204611858098318	36.81484102835864	18.6719434925569
35	18.535903103677004	30.490385590032954	34.568129560242774	16.40558174604727
36	15.966518059583912	32.90639176431047	35.02764376268154	16.099446413424072
37	15.620459763165243	32.2561542715793	34.93028152358122	17.19310444167424
38	15.339487456720486	33.70574019308844	33.47082600523177	17.483946344959303
39	18.064207506939837	29.6313750037789	33.869699982039116	18.434717507242148
40	14.64559255814615	29.844238200496857	35.10562246924865	20.404546772108343
41	15.712309254480886	30.672661928074646	32.72153688295013	20.893491934494328
42	15.715332374233773	29.923194975219307	34.61756645973116	19.743906190815764
43	15.54630441393267	31.130486740063272	34.66771468151433	18.655494164489724
44	13.513345295403257	35.18173395479191	33.07221877428499	18.232701975519845
45	15.434537898362713	32.68605968349714	33.926250104475464	17.95315231366468
46	15.334863861804305	32.0964624163974	33.033007132784306	19.535666589013985
47	14.811419568120668	30.634339439442464	35.590388613152705	18.96385237928416
48	15.885249487403371	28.709501309722175	36.32145010163017	19.08379910124428
49	15.888361522443105	31.49966301106284	34.31845543476908	18.293520031724974
50	14.224223013765865	31.272395537875248	34.82740653669622	19.675974911662664
51	14.774075147643833	29.215429291896438	35.92791104203381	20.082584518425914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1287.0
1	7891.5
2	14496.0
3	55440.5
4	96385.0
5	56642.5
6	16900.0
7	16894.0
8	16888.0
9	20322.5
10	23757.0
11	26481.5
12	29206.0
13	28157.0
14	27108.0
15	23986.0
16	20864.0
17	17879.0
18	14894.0
19	12895.0
20	10896.0
21	9972.5
22	9049.0
23	8727.5
24	8406.0
25	8933.0
26	10420.5
27	11381.0
28	12205.0
29	13029.0
30	15140.5
31	17252.0
32	20407.5
33	23563.0
34	26617.0
35	29671.0
36	32967.5
37	36264.0
38	39645.0
39	43026.0
40	47822.0
41	52618.0
42	59967.5
43	67317.0
44	69024.5
45	70732.0
46	70689.5
47	70647.0
48	71266.0
49	71885.0
50	73800.5
51	75716.0
52	70201.5
53	64687.0
54	56617.5
55	48548.0
56	43173.0
57	37798.0
58	33767.0
59	29736.0
60	24858.5
61	19981.0
62	16752.0
63	13523.0
64	11427.0
65	9331.0
66	7420.5
67	5510.0
68	4550.5
69	3591.0
70	3282.0
71	2973.0
72	2777.5
73	2582.0
74	2311.5
75	1411.5
76	782.0
77	606.5
78	431.0
79	346.0
80	261.0
81	176.5
82	92.0
83	72.5
84	53.0
85	34.5
86	16.0
87	13.5
88	11.0
89	9.0
90	7.0
91	7.5
92	8.0
93	5.5
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1124666.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.905151976426254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89764429676956	26.75036620463992
2	9.009207580215627	6.1091710438876445
3	3.307960539345494	3.364707144555898
4	1.7585976079743044	2.385020766549939
5	1.095551179853716	1.857241462544667
6	0.7952380866133127	1.6177600910440078
7	0.6030697933807344	1.4313021097876049
8	0.46043917838583065	1.2489008255257943
9	0.36399630741866523	1.1107215109699036
>10	2.9134051724797945	21.77352930413857
>50	0.635533829638299	14.964798237133106
>100	0.15394534378487695	7.8859089454110505
>500	0.0037877588978641468	0.9355727817834244
>1k	0.0010822168279611848	0.6780815769137242
>5k	2.705542069902962E-4	0.7519256880359574
>10k+	2.705542069902962E-4	7.134992307078786
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77781	6.915919926449275	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8197	0.7288386063062278	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3297	0.293153700743154	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1550	0.13781869461689072	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1441	0.12812692835028355	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08935986328385494	0.0
2	0.0	0.0	0.0	0.28764095295847836	0.0
3	0.0	0.0	0.0	0.47543003878484813	0.0
4	0.0	0.0	0.0	0.5978663887767568	0.0
5	0.0	0.0	0.0	1.2973629504226143	0.0
6	0.0	0.0	0.0	1.5095148248457764	0.0
7	0.0	0.0	0.0	2.083463001459989	0.0
8	0.0	0.0	0.0	2.780647765647757	0.0
9	0.0	0.0	0.0	3.2900434440091546	0.0
10	0.0	0.0	0.0	3.6452600149733345	0.0
11	0.0	0.0	0.0	4.045112059936017	0.0
12	0.0	0.0	0.0	4.370364179231879	0.0
13	0.0	0.0	0.0	4.510050094872611	0.0
14	0.0	0.0	0.0	4.623061424458461	0.0
15	0.0	0.0	0.0	5.007264378935613	0.0
16	0.0	0.0	0.0	5.4716689221511094	0.0
17	0.0	0.0	0.0	6.027922956682251	0.0
18	0.0	0.0	0.0	6.3111181452982485	0.0
19	0.0	0.0	0.0	6.627656566482849	0.0
20	0.0	0.0	0.0	6.994698870598026	0.0
21	0.0	0.0	0.0	7.378990740362028	0.0
22	0.0	0.0	0.0	7.76070406680739	0.0
23	0.0	0.0	0.0	8.069062281601827	0.0
24	5.334917210976414E-4	0.0	0.0	8.331273462521317	0.0
25	5.334917210976414E-4	0.0	0.0	8.59321789758026	0.0
26	5.334917210976414E-4	0.0	0.0	8.89152868496069	0.0
27	5.334917210976414E-4	0.0	0.0	9.176324348739982	0.0
28	6.224070079472484E-4	0.0	0.0	9.465476861574903	0.0
29	7.113222947968552E-4	0.0	0.0	9.812691056722619	0.0
30	7.113222947968552E-4	0.0	0.0	10.140432804050269	0.0
31	7.113222947968552E-4	0.0	0.0	10.441766711183586	0.0
32	7.113222947968552E-4	0.0	0.0	10.769419543224389	0.0
33	7.113222947968552E-4	0.0	0.0	11.090848305185718	0.0
34	7.113222947968552E-4	0.0	0.0	11.46802695200175	0.0
35	7.113222947968552E-4	8.89152868496069E-5	0.0	11.788210899947185	0.0
36	7.113222947968552E-4	8.89152868496069E-5	0.0	12.108572678466318	0.0
37	7.113222947968552E-4	8.89152868496069E-5	0.0	12.450451956403057	0.0
38	7.113222947968552E-4	8.89152868496069E-5	0.0	12.798910965566666	0.0
39	7.113222947968552E-4	8.89152868496069E-5	0.0	13.182224767175322	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	30	2.1654596E-6	45.000004	37
GCAACCG	30	2.1654596E-6	45.000004	35
ACCGTGG	30	2.1654596E-6	45.000004	1
ACCGTAG	30	2.1654596E-6	45.000004	38
TTACCGG	75	0.0	45.000004	1
CAATACG	30	2.1654596E-6	45.000004	23
TCGCCGT	35	1.2119017E-7	45.0	24
CAGCGTT	25	3.890753E-5	45.0	22
GGTACGC	25	3.890753E-5	45.0	7
CAACGTC	25	3.890753E-5	45.0	26
CGACGGT	295	0.0	45.0	27
GCGCGAA	20	7.0332224E-4	45.0	32
CGGTTAG	20	7.0332224E-4	45.0	30
CAACCGG	35	1.2119017E-7	45.0	1
ATTCGCG	20	7.0332224E-4	45.0	10
CGTTCGA	35	1.2119017E-7	45.0	13
TACGACC	20	7.0332224E-4	45.0	32
GTCGTCG	35	1.2119017E-7	45.0	24
AACGATG	20	7.0332224E-4	45.0	28
GCGATAT	145	0.0	45.0	8
>>END_MODULE