##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043455_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3747207
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.541683712695882	31.0	31.0	33.0	30.0	34.0
2	30.597735593470016	31.0	31.0	34.0	26.0	34.0
3	31.569973849856705	31.0	31.0	34.0	28.0	34.0
4	35.188130786476435	37.0	35.0	37.0	33.0	37.0
5	35.50662933753059	37.0	35.0	37.0	33.0	37.0
6	35.76293863669661	37.0	35.0	37.0	35.0	37.0
7	33.776214658010616	37.0	35.0	37.0	32.0	37.0
8	34.758076615463196	37.0	35.0	37.0	32.0	37.0
9	36.785942436593444	39.0	37.0	39.0	32.0	39.0
10	36.71667591355375	39.0	35.0	39.0	32.0	39.0
11	36.49156264919446	39.0	35.0	39.0	32.0	39.0
12	36.49358175302299	39.0	35.0	39.0	32.0	39.0
13	36.513915030581444	39.0	35.0	39.0	32.0	39.0
14	37.6919396232981	40.0	36.0	41.0	33.0	41.0
15	37.76706784546464	40.0	36.0	41.0	33.0	41.0
16	37.577548024435266	40.0	35.0	41.0	32.0	41.0
17	37.50996675657363	40.0	36.0	41.0	32.0	41.0
18	37.362486246423	39.0	35.0	41.0	32.0	41.0
19	37.21331380945862	39.0	35.0	41.0	32.0	41.0
20	36.958623315979075	38.0	35.0	41.0	32.0	41.0
21	36.869015509418084	38.0	35.0	41.0	32.0	41.0
22	36.82303993347578	38.0	35.0	40.0	32.0	41.0
23	36.78043246610075	38.0	35.0	40.0	32.0	41.0
24	36.6618945790825	38.0	35.0	40.0	32.0	41.0
25	36.56027676079811	38.0	35.0	40.0	31.0	41.0
26	36.29050036467161	38.0	35.0	40.0	31.0	41.0
27	36.14584969551989	38.0	35.0	40.0	31.0	41.0
28	35.97788459511311	37.0	35.0	40.0	30.0	41.0
29	35.88115601833579	37.0	35.0	40.0	30.0	41.0
30	35.73814150112337	37.0	34.0	40.0	30.0	41.0
31	35.55681097948418	37.0	34.0	40.0	29.0	41.0
32	35.461740704476696	37.0	34.0	40.0	28.0	41.0
33	35.15785997410872	37.0	34.0	40.0	26.0	41.0
34	35.058050969695564	37.0	34.0	40.0	26.0	41.0
35	34.910835990645836	37.0	34.0	40.0	25.0	41.0
36	34.818875498471265	37.0	34.0	40.0	25.0	41.0
37	34.69736152819954	37.0	34.0	40.0	24.0	41.0
38	34.58355596581667	36.0	34.0	40.0	24.0	41.0
39	34.47496095091624	36.0	34.0	40.0	23.0	41.0
40	34.36246142793819	36.0	34.0	40.0	23.0	41.0
41	34.25534618183623	35.0	33.0	40.0	23.0	41.0
42	34.12695882559997	35.0	33.0	40.0	23.0	41.0
43	33.93835061687278	35.0	33.0	40.0	23.0	41.0
44	33.705542021030595	35.0	33.0	40.0	22.0	41.0
45	33.46893379522402	35.0	33.0	40.0	21.0	41.0
46	33.455896351602675	35.0	33.0	39.0	22.0	41.0
47	33.41470086920739	35.0	33.0	39.0	22.0	41.0
48	33.32076183674934	35.0	33.0	39.0	22.0	41.0
49	33.268209629198495	35.0	33.0	39.0	22.0	41.0
50	33.19930204015951	35.0	33.0	39.0	22.0	41.0
51	32.76505754819523	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	3.0
10	6.0
11	9.0
12	25.0
13	36.0
14	89.0
15	206.0
16	471.0
17	1017.0
18	2046.0
19	3620.0
20	6136.0
21	9867.0
22	15016.0
23	22803.0
24	33887.0
25	50590.0
26	66892.0
27	81050.0
28	88861.0
29	95074.0
30	106165.0
31	122669.0
32	149875.0
33	197827.0
34	358859.0
35	376783.0
36	377691.0
37	428851.0
38	524840.0
39	625577.0
40	362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.52820594111828	17.68287687336195	24.41498428029196	28.37393290522781
2	31.161102122193945	20.85139678699362	27.282800229611016	20.704700861201424
3	26.373616402830162	21.776699285627934	32.02801446517366	19.82166984636824
4	23.84789524571234	24.543133058835554	29.138769221983203	22.470202473468905
5	22.211823366043028	33.74257146722879	27.2926742504484	16.75293091627978
6	83.19294877491423	2.2901857303319515	13.041286483506248	1.475579011247577
7	79.07948506714467	7.145962312730522	12.219047413180004	1.5555052069447992
8	78.15047313906064	3.6603528975047284	15.029167056957354	3.1600069064772778
9	47.08434842270523	17.020783746400987	21.09248301468267	14.802384816211115
10	30.725390937837165	17.577518402372753	28.835183111047773	22.861907548742302
11	26.59989159926313	14.7652371486283	38.12861152319581	20.50625972891276
12	22.925448207158023	16.66398466911489	38.185053561225736	22.22551356250135
13	19.356389972584914	19.765094375624297	40.962508876611295	19.916006775179486
14	19.916060148265093	17.9361054780267	41.16439257292164	20.983441800786558
15	24.677739980737652	15.241698683846394	38.65364256631673	21.426918769099228
16	24.710430995672244	16.242710904414942	34.61354016471468	24.433317935198133
17	24.438094826360008	19.40095649906717	34.998386798487516	21.16256187608531
18	26.083240130582592	22.236508418136495	31.022572278499695	20.657679172781222
19	27.155985778207608	20.826845167614174	30.407207287988093	21.609961766190125
20	27.046304087284213	22.018132438373435	33.06521363778408	17.87034983655827
21	24.463607161280386	18.22013035308698	33.78551011460002	23.530752371032612
22	26.117665770799427	19.570282613156945	32.32095264553039	21.99109897051324
23	26.32309877730267	18.91072470776234	33.374217116908675	21.391959398026316
24	27.773592438314726	17.373126171038855	31.51742084170957	23.33586054893685
25	22.278459663423984	19.182367026961682	32.521235149272506	26.017938160341824
26	20.76701393864817	19.396206294448103	34.207851341012116	25.628928425891605
27	21.884059247327407	21.132192590374643	34.208224952611374	22.775523209686575
28	24.408659569647472	22.33012481029204	31.394796177526356	21.86641944253413
29	23.725510760414355	19.71986068557195	35.15092707715373	21.403701476859965
30	28.883699245864985	19.668996134988006	30.725817922522026	20.72148669662498
31	29.931039304740835	20.255859897785204	29.779619860872376	20.03348093660158
32	29.858104983258198	18.87245620538177	29.045099456742047	22.224339354617985
33	24.860275933515283	21.665309655965096	32.43882710509454	21.03558730542508
34	24.86852207524164	23.193407783450446	30.979500198414446	20.95856994289347
35	26.86865177183967	26.361740891282494	27.66879438472441	19.100812952153433
36	25.869107311125326	24.574970104400425	27.31372993272056	22.242192651753694
37	25.392245477765172	28.200817302059907	26.371908464090723	20.0350287560842
38	24.795427634502175	24.02578774004212	26.6538251022695	24.5249595231862
39	24.35891585386129	21.5022548794342	30.56754003715301	23.571289229551503
40	22.111588711272155	22.76588936773442	30.511951968492802	24.610569952500622
41	22.05093019947924	26.004568202397145	26.634130433680337	25.31037116444328
42	21.548182419599453	21.092376268511455	28.550144147360957	28.809297164528143
43	22.473378172062553	20.164378429053958	32.08627118811424	25.275972210769247
44	25.07827830167909	22.052184466991015	30.28925276879553	22.58028446253436
45	25.254462857269427	23.114308870580142	30.047552750621996	21.583675521528434
46	23.30055425280749	23.476632062226614	29.53076784922744	23.69204583573846
47	22.292763650366794	24.64088586512568	31.28487430771772	21.78147617678981
48	24.402521664802613	23.51377972980943	30.44230009177502	21.641398513612938
49	22.60755810927979	21.87066260283993	32.29837583031842	23.223403457561858
50	21.802772037947197	22.770319333839844	33.5674810598934	21.85942756831955
51	21.241020311928324	20.654636906901594	32.69160203853163	25.41274074263845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1771.0
1	15107.0
2	28443.0
3	102527.0
4	176611.0
5	107363.0
6	38115.0
7	33861.0
8	29607.0
9	28935.0
10	28263.0
11	28165.5
12	28068.0
13	26840.0
14	25612.0
15	23804.0
16	21996.0
17	20359.5
18	18723.0
19	17197.5
20	15672.0
21	15593.5
22	15515.0
23	15412.5
24	15310.0
25	16533.0
26	18975.0
27	20194.0
28	21987.0
29	23780.0
30	27794.5
31	31809.0
32	36562.5
33	41316.0
34	46415.5
35	51515.0
36	57074.5
37	62634.0
38	66927.0
39	71220.0
40	78593.5
41	85967.0
42	97463.0
43	108959.0
44	122991.5
45	137024.0
46	148820.0
47	160616.0
48	185555.5
49	210495.0
50	211993.0
51	213491.0
52	197693.0
53	181895.0
54	192395.0
55	202895.0
56	217444.0
57	231993.0
58	244734.5
59	257476.0
60	263048.0
61	268620.0
62	257852.5
63	247085.0
64	228943.5
65	210802.0
66	178693.5
67	146585.0
68	123468.0
69	100351.0
70	86925.5
71	73500.0
72	67101.0
73	60702.0
74	50571.5
75	31617.0
76	22793.0
77	17010.5
78	11228.0
79	8446.0
80	5664.0
81	4231.5
82	2799.0
83	1966.5
84	1134.0
85	793.0
86	452.0
87	317.0
88	182.0
89	121.5
90	61.0
91	49.0
92	37.0
93	30.0
94	23.0
95	13.5
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3747207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.552698659929558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.66317193239497	18.29177285497998
2	9.109396960069525	4.291017631483918
3	2.8566311068655748	2.0184411492775776
4	1.3428530962471759	1.2651125728185248
5	0.8617065364156566	1.0147757192744788
6	0.561753398568128	0.7938485110599854
7	0.4532807194744565	0.7473188935896556
8	0.358147904775311	0.6748279741486437
9	0.2957848173864318	0.6269877604876428
>10	4.693144218234723	27.623531371143144
>50	1.464083157433866	23.984141636952096
>100	0.329471070780799	10.98373200685542
>500	0.006928501576411637	1.1315585192299313
>1k	0.0027957111624116867	1.225919477595055
>5k	3.646579777058722E-4	0.5791632851112296
>10k+	4.8621063694116295E-4	4.7478506359926556
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112816	3.0106690129475098	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	30101	0.8032916249355853	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11747	0.3134868183156148	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	11177	0.29827548891747907	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8621	0.230064685511102	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6459	0.17236837996940121	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5150	0.1374356954392965	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4697	0.1253466915491992	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4320	0.11528586491218659	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03421214787440352	0.0
2	0.0	0.0	0.0	0.11339112037312056	0.0
3	0.0	0.0	0.0	0.1846441896591248	0.0
4	0.0	0.0	0.0	0.23219960893540176	0.0
5	0.0	0.0	0.0	0.506003538101845	0.0
6	0.0	0.0	0.0	0.6111485167486077	0.0
7	0.0	0.0	0.0	0.9006975061692616	0.0
8	0.0	0.0	0.0	1.2128766838874927	0.0
9	0.0	0.0	0.0	1.5045605967324462	0.0
10	0.0	0.0	0.0	1.6942485429814793	0.0
11	0.0	0.0	0.0	1.8746495723348082	0.0
12	0.0	0.0	0.0	2.0211853788701823	0.0
13	0.0	0.0	0.0	2.0957475794638514	0.0
14	0.0	0.0	0.0	2.154164421661253	0.0
15	0.0	0.0	0.0	2.313589828370837	0.0
16	0.0	0.0	0.0	2.4881731913929497	2.6686542803746898E-5
17	0.0	0.0	0.0	2.7258969146887266	2.6686542803746898E-5
18	0.0	0.0	0.0	2.8624786407583036	2.6686542803746898E-5
19	0.0	0.0	0.0	3.019422198987139	2.6686542803746898E-5
20	0.0	0.0	0.0	3.1995296763696266	2.6686542803746898E-5
21	0.0	0.0	0.0	3.3865756548810886	2.6686542803746898E-5
22	0.0	0.0	0.0	3.573675006478158	2.6686542803746898E-5
23	0.0	0.0	0.0	3.7415867337993336	2.6686542803746898E-5
24	8.005962841124069E-5	0.0	0.0	3.8902574637590077	2.6686542803746898E-5
25	8.005962841124069E-5	0.0	0.0	4.033510825529521	2.6686542803746898E-5
26	8.005962841124069E-5	0.0	0.0	4.180233437864522	2.6686542803746898E-5
27	8.005962841124069E-5	0.0	0.0	4.337363801892983	2.6686542803746898E-5
28	8.005962841124069E-5	0.0	0.0	4.498390401170792	2.6686542803746898E-5
29	8.005962841124069E-5	0.0	0.0	4.674681702932344	2.6686542803746898E-5
30	8.005962841124069E-5	0.0	0.0	4.8499322295245495	2.6686542803746898E-5
31	8.005962841124069E-5	0.0	0.0	5.030359945420683	2.6686542803746898E-5
32	8.005962841124069E-5	0.0	0.0	5.2358996980951416	2.6686542803746898E-5
33	8.005962841124069E-5	0.0	0.0	5.436609186522122	2.6686542803746898E-5
34	8.005962841124069E-5	0.0	0.0	5.668355124229859	2.6686542803746898E-5
35	8.005962841124069E-5	0.0	0.0	5.878805200780207	2.6686542803746898E-5
36	8.005962841124069E-5	0.0	0.0	6.105907680040094	2.6686542803746898E-5
37	8.005962841124069E-5	0.0	0.0	6.398979293110842	2.6686542803746898E-5
38	8.005962841124069E-5	0.0	0.0	6.709530591718045	2.6686542803746898E-5
39	8.005962841124069E-5	0.0	0.0	7.0660361170333	2.6686542803746898E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTA	20	7.0353306E-4	45.0	41
CGTAATC	20	7.0353306E-4	45.0	33
CGTTTTT	31605	0.0	43.860943	1
TCTACGG	330	0.0	42.272728	1
CTTACTG	9295	0.0	40.812267	38
CACTTAC	9340	0.0	40.6879	36
CGCACTT	9335	0.0	40.66149	34
ACTTACT	9340	0.0	40.54336	37
CCGCACT	9440	0.0	40.209217	33
GCACTTA	9460	0.0	40.171776	35
ACTACGG	185	0.0	40.135136	1
GGGTACC	3390	0.0	39.955753	6
CCCGCAC	9560	0.0	39.93985	32
TATACGG	220	0.0	39.886364	1
ACCCGCA	9540	0.0	39.882072	31
ACGACCA	740	0.0	39.83108	27
CCAGTAG	3455	0.0	39.659912	25
GTTGATC	3560	0.0	39.56461	15
CATATGC	3330	0.0	39.527027	32
AACACGT	695	0.0	39.496403	40
>>END_MODULE