##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043454_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3437604
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54319636584086	31.0	31.0	33.0	30.0	34.0
2	30.58413185462898	31.0	31.0	34.0	26.0	34.0
3	31.557233177527138	31.0	31.0	34.0	28.0	34.0
4	35.171279763463154	37.0	35.0	37.0	32.0	37.0
5	35.49791395402146	37.0	35.0	37.0	33.0	37.0
6	35.78763493409945	37.0	35.0	37.0	35.0	37.0
7	33.74144782237861	37.0	35.0	37.0	32.0	37.0
8	34.74246655519367	37.0	35.0	37.0	31.0	37.0
9	36.734074081831416	39.0	37.0	39.0	32.0	39.0
10	36.72492555861583	39.0	35.0	39.0	32.0	39.0
11	36.48434549180185	39.0	35.0	39.0	32.0	39.0
12	36.46030636454926	39.0	35.0	39.0	32.0	39.0
13	36.443017287622425	39.0	35.0	39.0	32.0	39.0
14	37.673255849132126	40.0	36.0	41.0	33.0	41.0
15	37.75147137366608	40.0	36.0	41.0	33.0	41.0
16	37.55238299699442	40.0	35.0	41.0	32.0	41.0
17	37.47185045165179	40.0	35.0	41.0	32.0	41.0
18	37.34068467455821	39.0	35.0	41.0	32.0	41.0
19	37.1786578675147	38.0	35.0	41.0	32.0	41.0
20	36.9727531152512	38.0	35.0	41.0	32.0	41.0
21	36.86886534923743	38.0	35.0	41.0	32.0	41.0
22	36.80523963784078	38.0	35.0	40.0	32.0	41.0
23	36.76594337218597	38.0	35.0	40.0	32.0	41.0
24	36.6278253690652	38.0	35.0	40.0	32.0	41.0
25	36.5253115251204	38.0	35.0	40.0	31.0	41.0
26	36.25617755855532	38.0	35.0	40.0	31.0	41.0
27	36.13409048860776	38.0	35.0	40.0	31.0	41.0
28	35.96565369367734	37.0	35.0	40.0	30.0	41.0
29	35.82219563393573	37.0	35.0	40.0	30.0	41.0
30	35.652555675406475	37.0	34.0	40.0	30.0	41.0
31	35.50959185525733	37.0	34.0	40.0	29.0	41.0
32	35.447865722753406	37.0	34.0	40.0	28.0	41.0
33	35.17495150692168	37.0	34.0	40.0	26.0	41.0
34	35.03209910158355	37.0	34.0	40.0	25.0	41.0
35	34.942659189365614	37.0	34.0	40.0	25.0	41.0
36	34.832929563731014	37.0	34.0	40.0	25.0	41.0
37	34.73554574639778	37.0	34.0	40.0	24.0	41.0
38	34.599697347338434	36.0	34.0	40.0	24.0	41.0
39	34.471127564431505	36.0	34.0	40.0	23.0	41.0
40	34.3752997727487	36.0	34.0	40.0	23.0	41.0
41	34.27778097768097	36.0	33.0	40.0	23.0	41.0
42	34.172197262977356	35.0	33.0	40.0	23.0	41.0
43	33.99055417668818	35.0	33.0	40.0	23.0	41.0
44	33.77855273614995	35.0	33.0	40.0	22.0	41.0
45	33.54863707396198	35.0	33.0	40.0	21.0	41.0
46	33.54248569643275	35.0	33.0	40.0	22.0	41.0
47	33.51278797674194	35.0	33.0	39.0	22.0	41.0
48	33.42012721651476	35.0	33.0	39.0	22.0	41.0
49	33.37340862996436	35.0	33.0	39.0	22.0	41.0
50	33.3167150724749	35.0	33.0	39.0	22.0	41.0
51	32.89148517397583	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	4.0
11	6.0
12	15.0
13	37.0
14	69.0
15	158.0
16	398.0
17	815.0
18	1710.0
19	3226.0
20	5598.0
21	9089.0
22	14185.0
23	21133.0
24	31897.0
25	46791.0
26	61555.0
27	73497.0
28	80790.0
29	87849.0
30	97916.0
31	113885.0
32	137826.0
33	182530.0
34	334830.0
35	344119.0
36	336246.0
37	377119.0
38	476581.0
39	597389.0
40	336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.705137648199155	17.20797392602522	24.561380542959572	28.525507882816054
2	31.126069204015355	20.812170337246524	27.66839926879303	20.39336118994509
3	26.93239244543583	21.293813947156217	31.972152697052948	19.801640910355005
4	23.742816217342078	24.853880784406815	29.291215625767247	22.112087372483856
5	22.033747924426432	33.628189867128384	27.65850865893803	16.679553549507155
6	82.5904903531646	2.343114564679352	13.575531096659185	1.4908639854968753
7	78.16409336270263	7.493591466614537	12.729883954056373	1.6124312166264643
8	77.30023586195502	3.567310254467938	15.913351276063212	3.219102607513838
9	44.964603252730676	17.696279152572547	22.84442885218891	14.494688742507863
10	31.483818380476635	17.703958920224668	29.071731357073123	21.740491342225575
11	27.947139926530223	15.1164008419818	37.7056810499406	19.230778181547382
12	23.458257553807826	16.99526763408467	38.071429984372834	21.475044827734667
13	18.758181570652116	20.566388682349686	40.61424759803631	20.061182148961894
14	19.948720096904704	18.156657951293983	41.31552092678505	20.57910102501626
15	25.312456001331157	15.013393049344833	39.316715945175766	20.35743500414824
16	25.048987608811252	15.766097549339598	35.47883933111551	23.70607551073364
17	24.614091675480946	18.907704319636583	35.75030748160638	20.727896523276097
18	25.820600627646467	23.12471128146232	31.333568380767534	19.72111971012368
19	26.928639831696728	21.39885804182215	30.584878304772744	21.087623821708377
20	27.30654839824482	22.436150295380155	32.728667990844784	17.528633315530236
21	23.086312443201717	18.970916952621653	34.78134770613485	23.161422898041774
22	24.634483785799645	19.956545314701753	34.05965317703842	21.349317722460178
23	26.456915921671026	19.480196090067384	34.19719083408095	19.865697154180644
24	27.061144913724792	17.20006143814122	32.36326813676037	23.375525511373617
25	21.32328214651833	19.15258418363488	33.71778715640312	25.806346513443668
26	19.770223679050876	19.281569372155722	35.954199494764374	24.994007454029028
27	21.13908408298338	21.739967721703838	34.671794656976196	22.449153538336585
28	23.634804939719643	22.858101165812002	32.19760042168906	21.309493472779295
29	23.647546372415206	19.436008336038707	36.708969386817095	20.20747590472899
30	28.082786731688696	19.350425470763938	32.7589216209895	19.80786617655786
31	28.762940699394115	20.38207425869879	30.975441033929446	19.87954400797765
32	28.576473613598306	18.985025616679525	29.675669448837038	22.762831320885127
33	23.749216023718848	21.14769473156303	33.608757727766196	21.494331516951924
34	22.540321689176533	23.433298308938436	32.60256271519349	21.423817286691545
35	25.548550676575893	26.51977947430827	28.38587574368659	19.545794105429245
36	24.277723670323866	25.434081412518722	28.106902365717517	22.1812925514399
37	25.33383717263536	27.681867952213228	26.617871052046716	20.3664238231047
38	24.226787029570595	24.48909182093109	26.987168970015162	24.296952179483153
39	24.166687029686955	21.33715809034432	31.2580215754927	23.238133304476026
40	21.114822998809636	22.23438185433808	31.65527501131602	24.995520135536264
41	21.031014625302973	25.97384690034105	28.188354446876374	24.806784027479605
42	20.868197733072222	20.52371360982824	29.815999748662147	28.792088908437393
43	21.69176554367519	19.85699923551404	32.51892888186074	25.932306338950035
44	23.685770670501896	22.428208717467164	30.362863203556895	23.52315740847404
45	25.18643799576682	22.933909781347705	30.49240110262846	21.387251120257016
46	22.420412589699104	23.57956297467655	30.424737695208638	23.575286740415706
47	21.139927693823953	24.524465296177222	31.88491170012602	22.450695309872806
48	23.660258715081785	23.534298889575414	31.60553106175115	21.199911333591654
49	22.814815202681867	21.493284275908454	32.412575735890464	23.27932478551922
50	20.36802377469889	22.795499423435626	34.19765627454471	22.638820527320775
51	20.1185767761499	20.511728517886297	33.96255066028548	25.407144045678326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1705.0
1	14778.5
2	27852.0
3	95621.5
4	163391.0
5	99917.5
6	36444.0
7	32274.5
8	28105.0
9	27360.0
10	26615.0
11	26345.0
12	26075.0
13	25037.0
14	23999.0
15	22403.5
16	20808.0
17	19543.5
18	18279.0
19	17981.0
20	17683.0
21	16624.0
22	15565.0
23	15185.0
24	14805.0
25	15630.0
26	18866.0
27	21277.0
28	22757.0
29	24237.0
30	28833.0
31	33429.0
32	37281.5
33	41134.0
34	47105.5
35	53077.0
36	57123.0
37	61169.0
38	63187.5
39	65206.0
40	74991.5
41	84777.0
42	98715.5
43	112654.0
44	124158.0
45	135662.0
46	146819.0
47	157976.0
48	183703.0
49	209430.0
50	209167.0
51	208904.0
52	191816.5
53	174729.0
54	178809.0
55	182889.0
56	192518.0
57	202147.0
58	212716.0
59	223285.0
60	225530.5
61	227776.0
62	221445.0
63	215114.0
64	195891.5
65	176669.0
66	149211.0
67	121753.0
68	100540.0
69	79327.0
70	69517.5
71	59708.0
72	55707.0
73	51706.0
74	44250.0
75	28304.5
76	19815.0
77	14626.5
78	9438.0
79	7455.5
80	5473.0
81	3927.5
82	2382.0
83	1705.0
84	1028.0
85	725.5
86	423.0
87	299.5
88	176.0
89	181.0
90	186.0
91	119.5
92	53.0
93	33.0
94	13.0
95	8.0
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3437604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.125898285874158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.62479811431739	18.727679837675375
2	9.457657871065125	4.563489836398287
3	3.0086305937046687	2.177577470504641
4	1.459795423635156	1.408755036352254
5	0.8513110305781373	1.0269321666685423
6	0.577235369561276	0.8355793087826593
7	0.43299727677025435	0.7312513780193761
8	0.3603049136452787	0.6954143758805333
9	0.28148638536800696	0.6112000712022221
>10	4.1737063799532	25.333557083808223
>50	1.4093454194424853	23.629079139983187
>100	0.3519630996212973	12.44204011021622
>500	0.007524711995041094	1.2573240212193502
>1k	0.002205519033029286	0.997032595766024
>5k	5.189456548304202E-4	0.7706078516908241
>10k+	5.189456548304202E-4	4.792479715832203
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102923	2.994033053254534	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	27814	0.8091100661972699	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11652	0.3389570177367725	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10725	0.31199056086739485	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8040	0.23388383304185126	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5860	0.17046756985388659	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5399	0.15705706649166107	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5321	0.15478804423080728	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4691	0.1364613259700652	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.040667860521456226	0.0
2	2.909002898530488E-5	0.0	0.0	0.13067241020198953	0.0
3	2.909002898530488E-5	0.0	0.0	0.19929578857832375	0.0
4	2.909002898530488E-5	0.0	0.0	0.24877792788232733	0.0
5	2.909002898530488E-5	0.0	0.0	0.5339183919962858	0.0
6	2.909002898530488E-5	0.0	0.0	0.624592012343481	0.0
7	2.909002898530488E-5	0.0	0.0	0.9042926410371875	0.0
8	2.909002898530488E-5	0.0	0.0	1.2118906075278013	0.0
9	2.909002898530488E-5	0.0	0.0	1.4996782642794226	0.0
10	2.909002898530488E-5	0.0	0.0	1.6878325717563745	0.0
11	2.909002898530488E-5	0.0	0.0	1.8843066275231237	0.0
12	2.909002898530488E-5	0.0	0.0	2.0505852332031265	0.0
13	2.909002898530488E-5	0.0	0.0	2.138117130419909	0.0
14	2.909002898530488E-5	0.0	0.0	2.1995261816078875	0.0
15	2.909002898530488E-5	0.0	0.0	2.3828224542442933	0.0
16	2.909002898530488E-5	0.0	0.0	2.590496171170385	0.0
17	2.909002898530488E-5	0.0	0.0	2.8614697911685	0.0
18	2.909002898530488E-5	0.0	0.0	3.0204758896021766	0.0
19	2.909002898530488E-5	0.0	0.0	3.2113937498327325	0.0
20	2.909002898530488E-5	0.0	0.0	3.4215982992805456	0.0
21	2.909002898530488E-5	0.0	0.0	3.6311046880327114	0.0
22	2.909002898530488E-5	0.0	0.0	3.842356478523995	0.0
23	2.909002898530488E-5	0.0	0.0	4.0353688208414935	0.0
24	8.727008695591465E-5	0.0	0.0	4.2063891012461	0.0
25	8.727008695591465E-5	0.0	0.0	4.376565770810134	0.0
26	8.727008695591465E-5	0.0	0.0	4.560327483910305	0.0
27	8.727008695591465E-5	0.0	0.0	4.758692391561099	0.0
28	8.727008695591465E-5	0.0	0.0	4.965202507327779	0.0
29	8.727008695591465E-5	0.0	0.0	5.16618551758725	0.0
30	8.727008695591465E-5	0.0	0.0	5.373684694339429	0.0
31	8.727008695591465E-5	0.0	0.0	5.584529224424919	0.0
32	8.727008695591465E-5	0.0	0.0	5.838077917060836	0.0
33	8.727008695591465E-5	0.0	0.0	6.07597617410266	0.0
34	8.727008695591465E-5	0.0	0.0	6.350178787318144	0.0
35	8.727008695591465E-5	0.0	0.0	6.59116058743241	0.0
36	8.727008695591465E-5	0.0	0.0	6.865275930560937	0.0
37	8.727008695591465E-5	0.0	0.0	7.197454971544134	0.0
38	1.1636011594121952E-4	0.0	0.0	7.584701437396512	0.0
39	1.1636011594121952E-4	0.0	0.0	8.020906422031159	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	20	7.0352474E-4	45.000004	16
ACAATCG	20	7.0352474E-4	45.000004	38
ACGGGTA	345	0.0	44.347828	4
CGTTTTT	30160	0.0	43.63478	1
ACGTAGG	320	0.0	42.89063	1
GCGATAT	165	0.0	42.272728	8
CGAAACG	75	0.0	42.0	13
CGCACTT	8670	0.0	40.97751	34
TCACGAC	710	0.0	40.880283	24
ACATACG	540	0.0	40.833332	16
CCGCACT	8760	0.0	40.556507	33
CACTTAC	8760	0.0	40.556507	36
CTTACTG	8665	0.0	40.53376	38
GCACTTA	8775	0.0	40.435898	35
ACTTACT	8720	0.0	40.40711	37
ACCCGCA	8785	0.0	40.287422	31
CACGACG	715	0.0	40.27972	25
ACGGGAT	770	0.0	40.032467	4
CCCGCAC	8905	0.0	40.02246	32
AATGCGG	480	0.0	39.843746	1
>>END_MODULE