FastQCFastQC Report
Thu 26 May 2016
SRR1043453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043453_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2103471
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680613.235651929596367No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC178900.8504990085434979No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79290.3769483867379203No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC70960.33734717521658253No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA50000.2377023500680542No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC44800.21298130566097653No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30330.14419024555128165No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC29040.13805752491952586No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC21080.10021531078869164No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA253.8919134E-545.00000443
AATATCG302.1663654E-645.00000424
TAACACG253.8919134E-545.00000413
CGTAAAT207.034623E-445.00000435
ATCGGTA253.8919134E-545.00000426
GCGTAAT207.034623E-445.00000425
ATCGATA207.034623E-445.00000434
ATCGAAT253.8919134E-545.00000413
ACGTAGG2000.043.8750041
CGTTTTT179500.043.1573831
ACTACGG1500.042.0000041
TAATCGT1500.042.00000420
TAGTAGG4350.041.896551
TAACGGG6000.041.6250042
CGGTCTA5650.041.4159330
CACGACG5600.041.3839325
ACGATAC603.6379788E-1241.25000444
ACGGGTA2050.040.609764
ACGGGAT4750.040.2631574
TCACGAC5900.040.04237424