##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043452_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1851856
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.543454782661286	31.0	31.0	33.0	30.0	34.0
2	30.527713817921047	31.0	31.0	34.0	26.0	34.0
3	31.460513668449384	31.0	31.0	34.0	28.0	34.0
4	35.079249682480715	37.0	35.0	37.0	32.0	37.0
5	35.409092283633285	37.0	35.0	37.0	33.0	37.0
6	35.88382304023639	37.0	35.0	37.0	35.0	37.0
7	33.69948581315178	37.0	35.0	37.0	32.0	37.0
8	34.763406549969325	37.0	35.0	37.0	31.0	37.0
9	36.82642062881779	39.0	37.0	39.0	32.0	39.0
10	36.68969509508299	39.0	35.0	39.0	32.0	39.0
11	36.7517220561426	39.0	35.0	39.0	33.0	39.0
12	36.8184756266146	39.0	37.0	39.0	33.0	39.0
13	36.84859513914689	39.0	37.0	39.0	33.0	39.0
14	38.12145598793859	40.0	37.0	41.0	33.0	41.0
15	38.216648594707145	40.0	37.0	41.0	33.0	41.0
16	38.18828299824608	40.0	37.0	41.0	33.0	41.0
17	38.09684716306236	40.0	37.0	41.0	33.0	41.0
18	37.82784568562566	39.0	37.0	41.0	33.0	41.0
19	37.5576216509275	39.0	36.0	41.0	33.0	41.0
20	37.16440911172359	39.0	35.0	41.0	33.0	41.0
21	37.11553274120666	38.0	35.0	41.0	33.0	41.0
22	37.056086434366385	38.0	35.0	40.0	33.0	41.0
23	36.966000596158665	38.0	35.0	40.0	33.0	41.0
24	36.81952160427161	38.0	35.0	40.0	32.0	41.0
25	36.74921106176722	38.0	35.0	40.0	32.0	41.0
26	36.491307099472095	38.0	35.0	40.0	32.0	41.0
27	36.37590827796546	38.0	35.0	40.0	31.0	41.0
28	36.27198659075004	38.0	35.0	40.0	31.0	41.0
29	36.195740921540335	38.0	35.0	40.0	31.0	41.0
30	35.96693857405759	38.0	35.0	40.0	30.0	41.0
31	35.5031341529795	38.0	35.0	40.0	29.0	41.0
32	35.271585911647556	38.0	35.0	40.0	26.0	41.0
33	34.95032065128174	38.0	34.0	40.0	24.0	41.0
34	34.733671516575804	38.0	34.0	40.0	23.0	41.0
35	34.53091439075177	38.0	34.0	40.0	21.0	41.0
36	34.348794938699335	38.0	34.0	40.0	20.0	41.0
37	34.21439733974996	37.0	34.0	40.0	18.0	41.0
38	34.08863000146879	37.0	34.0	40.0	18.0	41.0
39	33.995939749094966	37.0	34.0	40.0	18.0	41.0
40	33.91377029315454	37.0	33.0	40.0	18.0	41.0
41	33.814505015508765	37.0	33.0	40.0	18.0	41.0
42	33.713940500773276	37.0	33.0	40.0	18.0	41.0
43	33.55658377325235	37.0	33.0	40.0	16.0	41.0
44	33.36030177292403	36.0	33.0	40.0	15.0	41.0
45	33.14326275909142	36.0	33.0	40.0	15.0	41.0
46	33.15671466895914	36.0	33.0	40.0	15.0	41.0
47	33.11669751859756	36.0	33.0	40.0	15.0	41.0
48	33.04995096810983	36.0	33.0	40.0	15.0	41.0
49	32.967192373489084	35.0	33.0	40.0	13.0	41.0
50	32.85637382172264	35.0	33.0	40.0	12.0	41.0
51	32.44253926871204	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	8.0
13	14.0
14	45.0
15	87.0
16	180.0
17	479.0
18	951.0
19	1824.0
20	3284.0
21	5475.0
22	8678.0
23	13887.0
24	22833.0
25	34522.0
26	44048.0
27	47576.0
28	46636.0
29	46321.0
30	48835.0
31	55956.0
32	66690.0
33	85541.0
34	130826.0
35	175085.0
36	196824.0
37	188002.0
38	243241.0
39	383862.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.77109451274829	18.115717420792976	25.005399987904024	32.107788078554705
2	33.85614216224156	19.622368045895577	28.092411072999194	18.42907871886367
3	20.55170596417864	21.08997675845206	40.464323359915674	17.893993917453624
4	21.933670868577256	23.65470101346973	36.866905418131864	17.544722699821154
5	19.79851565132494	29.580917738744265	34.777326098789544	15.843240511141254
6	75.65264253808071	1.740632100984094	21.156072610397352	1.4506527505378388
7	71.83350109295755	7.347061542582145	18.984575474550937	1.8348618899093665
8	70.43063823537035	4.479343966269515	22.439595735305552	2.6504220630545787
9	32.926318245047135	32.167781944168446	26.358744956411297	8.547154854373126
10	19.6708059373947	22.568169447300438	45.6129418270103	12.148082788294554
11	19.738629785469282	20.63529777693298	44.380232588279	15.245839849318738
12	19.099379217390553	21.928864879342672	44.29269878435472	14.679057118912054
13	15.65283693764526	26.19361332630615	42.72745829049343	15.426091445555162
14	14.748068964325519	24.229043726942052	46.1707605774963	14.852126731236122
15	16.94230004924789	25.371735167313226	43.571584399650945	14.114380383787942
16	18.21394320076723	23.429845517146042	41.286957517215164	17.06925376487157
17	16.65556069154405	23.156984128355553	43.50786454238342	16.67959063771697
18	18.468552630442108	25.21707951374189	41.78062441140132	14.533743444414684
19	19.315594733067798	25.55009676778324	40.21030792891024	14.924000570238722
20	17.13362162068757	26.606766400843263	42.03787983514917	14.221732143319999
21	17.28897927268643	24.070230082684617	41.900774142265924	16.740016502363034
22	15.62680899594785	25.8766880362188	41.19456372417726	17.301939243656093
23	14.24009210219369	24.665902748377842	44.27450082511815	16.819504324310312
24	15.91614034784562	25.30531531609369	42.53997071046561	16.23857362559508
25	14.635965215437919	27.198767074761754	41.04422806092914	17.121039648871186
26	14.028952575146231	27.757611822949517	41.332209415850905	16.881226186053343
27	14.686779101614814	26.78210400808702	42.03458584252771	16.496531047770453
28	15.166730026524741	25.825604150646704	41.67975263735409	17.327913185474465
29	15.230341884034178	26.70223818698646	41.466507115024065	16.600912813955297
30	16.76712444164125	27.54506829904701	38.990936660301884	16.69687059900986
31	17.374029082174854	27.45893849197778	39.235232113080066	15.9318003127673
32	17.762234212595363	28.1137410252201	38.407845966425036	15.716178795759497
33	14.948570515202045	28.162880915146747	40.33396765191246	16.554580917738747
34	15.300433727028452	29.394510156297248	37.85342920831857	17.451626908355724
35	18.202981225322056	28.803913479233806	36.57843806429874	16.4146672311454
36	16.715932556311074	29.39407815726493	37.44265212845923	16.447337157964768
37	16.016742122497647	30.675981285801917	36.28694671723935	17.020329874461083
38	15.90604237046509	29.444784043683743	36.532862166388746	18.11631141946242
39	17.510000777598258	27.966267355561126	37.20235266673003	17.32137920011059
40	15.317767688200378	27.966915354109606	37.934645026395145	18.780671931294872
41	16.71155856610881	29.1611226790852	34.93387174812728	19.193447006678703
42	16.02149411185319	28.72302166043148	35.908569564804175	19.346914662911153
43	16.801684364227025	29.062680899594785	36.63432793910542	17.501306797072775
44	16.911466118315897	29.81700520990833	35.20819113365186	18.063337538123914
45	16.22275166103628	29.943148927346403	35.35993079375503	18.474168617862297
46	16.00772414269792	29.47064998574403	35.30274492185138	19.218880949706673
47	15.957990254101832	28.983139077768467	36.600092015793884	18.45877865233582
48	16.782838406441968	27.807021712271364	36.423080412299875	18.98705946898679
49	16.046280056332673	28.900303263320694	36.322370637889776	18.731046042456864
50	16.135811855781444	27.805347716021117	36.5397741509059	19.51906627729154
51	15.665850908493963	27.547552293482862	36.516824202313785	20.269772595709384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1291.0
1	10935.0
2	20579.0
3	79308.5
4	138038.0
5	83890.0
6	29742.0
7	28348.0
8	26954.0
9	29834.0
10	32714.0
11	35738.0
12	38762.0
13	38000.5
14	37239.0
15	34808.0
16	32377.0
17	29933.0
18	27489.0
19	25481.5
20	23474.0
21	22716.0
22	21958.0
23	22560.5
24	23163.0
25	24162.5
26	27572.5
27	29983.0
28	32364.5
29	34746.0
30	38088.0
31	41430.0
32	44493.5
33	47557.0
34	52366.5
35	57176.0
36	62019.5
37	66863.0
38	71130.5
39	75398.0
40	81376.5
41	87355.0
42	98121.5
43	108888.0
44	119725.5
45	130563.0
46	137661.5
47	144760.0
48	135567.0
49	126374.0
50	112866.0
51	99358.0
52	86313.0
53	73268.0
54	65668.0
55	58068.0
56	51918.5
57	45769.0
58	41611.0
59	37453.0
60	33738.5
61	30024.0
62	27806.5
63	25589.0
64	22801.5
65	20014.0
66	16228.0
67	12442.0
68	9597.0
69	6752.0
70	5528.0
71	4304.0
72	3913.5
73	3523.0
74	2994.5
75	1899.0
76	1332.0
77	1018.0
78	704.0
79	565.0
80	426.0
81	304.5
82	183.0
83	133.0
84	83.0
85	56.5
86	30.0
87	21.5
88	13.0
89	8.0
90	3.0
91	4.5
92	6.0
93	8.0
94	10.0
95	5.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1851856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.0421335870713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2449404231886	29.38562342504304
2	9.756612020769463	7.4232467570267495
3	3.476500895814684	3.9676053448246575
4	1.7893906615765645	2.7228895434861418
5	1.1564859134121703	2.1997595804795975
6	0.8572438542059727	1.9566831131039693
7	0.6489123367145834	1.7280206859716338
8	0.5177052230297958	1.5755689002579225
9	0.42837006228249486	1.4666500020647448
>10	3.795431866531528	29.61134774228691
>50	0.27108627205644104	6.753547250919645
>100	0.052593087023524235	3.268177266964259
>500	0.003397806814551113	0.9135859330371483
>1k	0.001034115117472078	0.8463146263291348
>5k	0.0	0.0
>10k+	2.954614621348794E-4	6.180979828204457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99172	5.355276004181751	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10810	0.5837386924253289	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4353	0.23506147346229944	No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	2667	0.14401767740040264	No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	2521	0.13613369506052306	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCC	2009	0.10848575699190434	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17971159744602172	0.0
2	0.0	0.0	0.0	0.5238528265696685	0.0
3	0.0	0.0	0.0	0.8213921600815615	0.0
4	0.0	0.0	0.0	1.0170337218444632	0.0
5	0.0	0.0	0.0	1.9637056012994532	0.0
6	0.0	0.0	0.0	2.2489869622692047	0.0
7	0.0	0.0	0.0	3.021725231335482	0.0
8	0.0	0.0	0.0	3.9205532179607916	0.0
9	0.0	0.0	0.0	4.622605645363354	0.0
10	0.0	0.0	0.0	5.137170492738096	0.0
11	0.0	0.0	0.0	5.801099005538227	0.0
12	0.0	0.0	0.0	6.342825792070226	0.0
13	0.0	0.0	0.0	6.593601230333244	0.0
14	0.0	0.0	0.0	6.779684813506018	0.0
15	0.0	0.0	0.0	7.302457642494881	0.0
16	0.0	0.0	0.0	7.962876163157395	0.0
17	0.0	0.0	0.0	8.785942319489205	0.0
18	0.0	0.0	0.0	9.280041212707683	0.0
19	0.0	0.0	0.0	9.812804019318996	0.0
20	2.159995161610838E-4	0.0	0.0	10.344918827381827	0.0
21	2.159995161610838E-4	0.0	0.0	10.898363587665564	0.0
22	2.6999939520135476E-4	0.0	0.0	11.442304369238213	0.0
23	2.6999939520135476E-4	0.0	0.0	11.877813393697998	0.0
24	4.8599891136243855E-4	0.0	0.0	12.250898557987231	0.0
25	4.8599891136243855E-4	0.0	0.0	12.636187694939563	0.0
26	4.8599891136243855E-4	0.0	0.0	13.051662764275408	0.0
27	5.939986694429804E-4	0.0	0.0	13.451693868205735	0.0
28	6.479985484832514E-4	0.0	0.0	13.879588909720843	0.0
29	6.479985484832514E-4	0.0	0.0	14.356893840557797	0.0
30	6.479985484832514E-4	0.0	0.0	14.804930836954926	0.0
31	6.479985484832514E-4	0.0	0.0	15.226993891533683	0.0
32	6.479985484832514E-4	0.0	0.0	15.638094970667266	0.0
33	6.479985484832514E-4	0.0	0.0	16.05562203540664	0.0
34	6.479985484832514E-4	0.0	0.0	16.52191099091938	0.0
35	6.479985484832514E-4	0.0	0.0	16.95812201380669	0.0
36	6.479985484832514E-4	0.0	0.0	17.437694939563336	0.0
37	6.479985484832514E-4	0.0	0.0	17.912083876932115	0.0
38	6.479985484832514E-4	0.0	0.0	18.426378724911658	0.0
39	7.019984275235223E-4	0.0	0.0	18.928037601195772	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCGT	30	2.1662236E-6	45.000004	33
CAACGAT	25	3.8917333E-5	45.0	34
CCCATCG	25	3.8917333E-5	45.0	28
CGTTTTT	27100	0.0	44.46863	1
CGGTCTA	155	0.0	43.54839	30
CGTTTTA	1440	0.0	43.125004	1
CTCGCGC	105	0.0	42.857143	32
TACGCGG	290	0.0	41.89655	1
CTAGCGG	355	0.0	41.830982	1
AGTACGG	140	0.0	41.785713	1
GCGATTG	260	0.0	41.538464	8
GCGATCG	65	0.0	41.538464	8
ACTGCGG	245	0.0	41.32653	1
AATACGG	55	6.184564E-11	40.909092	1
ACGGTCT	160	0.0	40.78125	29
CTTACGG	105	0.0	40.714287	1
CGACGGT	155	0.0	40.645164	27
ACGCGAG	45	1.929402E-8	40.000004	2
CGGGATC	980	0.0	39.94898	5
GTAGCGG	335	0.0	39.626865	1
>>END_MODULE