##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043451_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1193771
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52696371414618	31.0	31.0	33.0	30.0	34.0
2	30.545086955538373	31.0	31.0	34.0	26.0	34.0
3	31.46730235530935	31.0	31.0	34.0	28.0	34.0
4	35.08303937689892	37.0	35.0	37.0	32.0	37.0
5	35.39573419022577	37.0	35.0	37.0	33.0	37.0
6	35.83490887280726	37.0	35.0	37.0	35.0	37.0
7	33.73915935300824	37.0	35.0	37.0	32.0	37.0
8	34.77511013418822	37.0	35.0	37.0	31.0	37.0
9	36.75418149712131	39.0	37.0	39.0	32.0	39.0
10	36.727994732658104	39.0	35.0	39.0	32.0	39.0
11	36.513757663739526	39.0	35.0	39.0	32.0	39.0
12	36.46732413503092	39.0	35.0	39.0	32.0	39.0
13	36.50352370764577	39.0	35.0	39.0	32.0	39.0
14	37.86952355183699	40.0	36.0	41.0	33.0	41.0
15	38.01379661593388	40.0	36.0	41.0	33.0	41.0
16	37.97350161798201	40.0	36.0	41.0	33.0	41.0
17	37.93139136400532	40.0	36.0	41.0	33.0	41.0
18	37.60639184567224	39.0	36.0	41.0	33.0	41.0
19	37.273596862379804	38.0	35.0	41.0	33.0	41.0
20	36.92529471732853	38.0	35.0	41.0	32.0	41.0
21	36.82691571499056	38.0	35.0	41.0	32.0	41.0
22	36.735934278852476	38.0	35.0	40.0	32.0	41.0
23	36.64063291870887	38.0	35.0	40.0	32.0	41.0
24	36.4587420870502	37.0	35.0	40.0	32.0	41.0
25	36.350376244690146	37.0	35.0	40.0	32.0	41.0
26	36.06633516813526	37.0	35.0	40.0	31.0	41.0
27	35.95482634441614	36.0	35.0	40.0	31.0	41.0
28	35.852614948763204	36.0	35.0	40.0	31.0	41.0
29	35.775291911095174	36.0	35.0	40.0	31.0	41.0
30	35.586100684302096	36.0	35.0	40.0	30.0	41.0
31	35.065328274853385	36.0	34.0	40.0	27.0	41.0
32	34.846906148666704	36.0	34.0	40.0	25.0	41.0
33	34.642827644497984	36.0	34.0	40.0	24.0	41.0
34	34.40274558520856	36.0	34.0	40.0	22.0	41.0
35	34.16425512095704	36.0	34.0	40.0	21.0	41.0
36	33.98320364626046	36.0	33.0	40.0	20.0	41.0
37	33.89379537616511	36.0	33.0	40.0	20.0	41.0
38	33.78177556667066	35.0	33.0	40.0	18.0	41.0
39	33.66640419309901	35.0	33.0	40.0	18.0	41.0
40	33.59526575867566	35.0	33.0	40.0	18.0	41.0
41	33.51698357557689	35.0	33.0	40.0	18.0	41.0
42	33.42709196319897	35.0	33.0	40.0	18.0	41.0
43	33.27857352875887	35.0	33.0	40.0	17.0	41.0
44	33.09176215538826	35.0	33.0	40.0	16.0	41.0
45	32.91981963039812	35.0	33.0	40.0	15.0	41.0
46	32.929000620722064	35.0	33.0	40.0	15.0	41.0
47	32.90543161125542	35.0	33.0	40.0	15.0	41.0
48	32.836574183825874	35.0	33.0	40.0	15.0	41.0
49	32.762632866772606	35.0	33.0	40.0	15.0	41.0
50	32.64736033962963	35.0	33.0	40.0	15.0	41.0
51	32.240087085379024	35.0	31.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	1.0
9	1.0
10	1.0
11	2.0
12	5.0
13	20.0
14	24.0
15	80.0
16	170.0
17	375.0
18	795.0
19	1481.0
20	2459.0
21	4001.0
22	6120.0
23	9507.0
24	15508.0
25	23411.0
26	28808.0
27	31436.0
28	30719.0
29	30433.0
30	32510.0
31	37509.0
32	45066.0
33	58566.0
34	95606.0
35	135306.0
36	136881.0
37	108137.0
38	137049.0
39	221678.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.98150817870429	17.432405377580793	25.22502221950441	32.36106422421051
2	32.98404802931215	19.95659133954502	28.38735402351037	18.67200660763245
3	20.442949275866145	20.850732678210477	39.96310850238446	18.743209543538917
4	21.50135997607581	24.639231477393906	37.36378250099894	16.495626045531345
5	19.281252434512147	29.630305979957626	35.13529814344627	15.95314344208395
6	75.85156617140139	1.911673176848826	20.77316336215237	1.4635972895974185
7	72.36278984830425	7.348980667146379	18.843563799087097	1.444665685462287
8	71.39995861852901	4.09374997382245	21.714466174835877	2.7918252328126583
9	37.65152613022095	23.04001353693464	27.759511665135108	11.5489486677093
10	27.657817119028692	25.40093535527333	36.189017826702106	10.75222969899587
11	25.413835651896388	22.476337589035083	39.46318012416117	12.646646634907366
12	18.732319682753225	28.362977488982395	38.16988350362004	14.734819324644342
13	14.526404142838114	30.76544831462651	37.99908022560441	16.70906731693097
14	13.23495042181457	25.266906299449392	44.91874907331473	16.57939420542131
15	15.99511129018882	23.396530825426318	44.01162366986633	16.59673421451853
16	16.99404659687662	24.855018257270448	37.2806844863881	20.87025065946484
17	17.72668292327423	24.440868474774476	41.70305695145887	16.12939165049243
18	20.568433979381307	27.08827739993684	36.8808590592333	15.462429561448552
19	21.31011726704703	26.578213074366857	37.04462581181818	15.06704384676793
20	16.70194702334032	29.470811403527144	37.79619374235092	16.031047830781617
21	17.288826751529395	25.704259862234885	37.74124182946311	19.265671556772617
22	14.779132681226132	29.663645707593833	37.12965049410649	18.427571117073544
23	14.49582876447828	26.172775180499443	44.60445093740759	14.726945117614685
24	15.141597509069998	27.50594544514819	41.28765064656454	16.06480639921727
25	14.956302339393401	30.993046405047536	37.84176362133106	16.208887634228006
26	14.18119555593158	31.540806402567995	37.522188091350856	16.75580995014957
27	14.105217834911384	29.34247858257572	40.8382344687549	15.714069113758
28	13.601938730292492	27.374596970440727	40.29432780658937	18.729136492677405
29	14.412982054347106	31.075893115178705	38.956298988667	15.55482584180718
30	18.124246610111992	30.422669004356784	36.20811696715702	15.24496741837421
31	17.911140411351926	29.424152538468434	36.008748746618906	16.655958303560734
32	16.849043912107096	32.44298948458289	34.857187852611595	15.850778750698415
33	14.368501161445538	30.365120278512375	35.98470728473049	19.281671275311595
34	14.277780244284708	31.583193091472317	36.485808417192246	17.653218247050734
35	19.355638560494434	30.48909715514952	34.19751359347815	15.957750690877898
36	15.138163014514507	33.72548001249821	34.75993301897935	16.37642395400793
37	16.13307744952759	32.7099586101522	34.86941800395553	16.28754593636468
38	15.788120167100725	34.767555921529336	33.355140977624686	16.08918293374525
39	17.98259465173806	31.24812045191247	33.64355475212582	17.125730144223642
40	14.818336180054633	31.189063899190046	34.579328866256596	19.413271054498725
41	15.906903417824692	31.330967162043642	32.82924446983551	19.932884950296163
42	15.606343260139507	30.90450346004384	34.295354804229625	19.193798475587027
43	15.805543944357838	31.614522383271165	34.541716962466005	18.038216709904997
44	14.152714381568995	35.94843567149814	32.42615208444501	17.472697862487866
45	16.44620283119627	33.317445305674205	33.625041988790144	16.611309874339383
46	15.561778599078046	33.28728876811382	32.46183732055813	18.689095312250004
47	15.150141861378774	31.5082205883708	34.85534495309402	18.486292597156407
48	15.286683961999412	30.480720339160527	35.608336942344884	18.624258756495173
49	15.764078705212306	32.573081436892	33.96798883537965	17.694851022516044
50	14.813310090461234	32.37220538947587	34.167105751438086	18.647378768624804
51	14.396312190529004	31.28899931393877	35.37110551353651	18.94358298199571
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1033.0
1	7546.5
2	14060.0
3	55260.5
4	96461.0
5	57920.0
6	19379.0
7	19121.5
8	18864.0
9	21950.0
10	25036.0
11	27302.0
12	29568.0
13	28837.5
14	28107.0
15	25122.5
16	22138.0
17	19054.5
18	15971.0
19	14289.0
20	12607.0
21	11807.5
22	11008.0
23	10254.5
24	9501.0
25	9879.5
26	11266.0
27	12274.0
28	13574.5
29	14875.0
30	16227.5
31	17580.0
32	19287.5
33	20995.0
34	27462.5
35	33930.0
36	38449.5
37	42969.0
38	44734.0
39	46499.0
40	52565.5
41	58632.0
42	67608.5
43	76585.0
44	84254.0
45	91923.0
46	91397.0
47	90871.0
48	84966.0
49	79061.0
50	72632.5
51	66204.0
52	61148.0
53	56092.0
54	50520.0
55	44948.0
56	40869.5
57	36791.0
58	33171.0
59	29551.0
60	24636.0
61	19721.0
62	16573.0
63	13425.0
64	10778.0
65	8131.0
66	6823.0
67	5515.0
68	4581.0
69	3647.0
70	3126.0
71	2605.0
72	2552.5
73	2500.0
74	2304.0
75	1565.0
76	1022.0
77	903.5
78	785.0
79	525.0
80	265.0
81	200.0
82	135.0
83	95.0
84	55.0
85	49.0
86	43.0
87	32.5
88	22.0
89	14.5
90	7.0
91	6.5
92	6.0
93	5.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1193771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.46737606155482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.43237181420169	27.817904265339845
2	9.134874296001968	6.479800438626652
3	3.341450288796709	3.5553742195123155
4	1.7760791294295537	2.5197146559422756
5	1.1954647309710231	2.119999859083737
6	0.8734856174165143	1.8588145726362546
7	0.5982944744553036	1.485395458473959
8	0.4664776834351724	1.3235791538174546
9	0.38008634869494434	1.2132598918524162
>10	3.141989982601865	24.133259535801386
>50	0.5454887173748189	13.161415343033958
>100	0.10953591984609876	5.83713986926124
>500	0.003178497670403794	0.7494141120307264
>1k	7.334994624008755E-4	0.5033093619794418
>5k	2.4449982080029185E-4	0.6894054837588839
>10k+	2.4449982080029185E-4	6.552213778849528
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75558	6.3293546249657595	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7950	0.6659568711251991	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3200	0.26805811164787885	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1505	0.12607108063439304	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08393569620974208	0.0
2	0.0	0.0	0.0	0.26428854445283056	0.0
3	0.0	0.0	0.0	0.4370184901459325	0.0
4	0.0	0.0	0.0	0.5485976791193621	0.0
5	0.0	0.0	0.0	1.196795700347889	0.0
6	0.0	0.0	0.0	1.4329381430776924	0.0
7	0.0	0.0	0.0	1.9767610370833266	0.0
8	0.0	0.0	0.0	2.6015039735426644	0.0
9	0.0	0.0	0.0	3.084176110828626	0.0
10	0.0	0.0	0.0	3.4000658417736735	0.0
11	0.0	0.0	0.0	3.7527297949104144	0.0
12	0.0	0.0	0.0	4.056054301871967	0.0
13	0.0	0.0	0.0	4.202564813519511	0.0
14	0.0	0.0	0.0	4.31439530697261	0.0
15	0.0	0.0	0.0	4.666472882990122	0.0
16	0.0	0.0	0.0	5.101816009938254	0.0
17	0.0	0.0	0.0	5.594121485611562	0.0
18	0.0	0.0	0.0	5.870305108768767	0.0
19	0.0	0.0	0.0	6.1809174456407465	0.0
20	0.0	0.0	0.0	6.557957933305467	0.0
21	0.0	0.0	0.0	6.937343929447105	0.0
22	0.0	0.0	0.0	7.338928487959584	0.0
23	8.376815988996214E-5	0.0	0.0	7.65573966866342	0.0
24	2.5130447966988643E-4	0.0	0.0	7.952446490993666	0.0
25	2.5130447966988643E-4	0.0	0.0	8.244294760050295	0.0
26	2.5130447966988643E-4	0.0	0.0	8.545776367494268	0.0
27	2.5130447966988643E-4	0.0	0.0	8.846504061499232	0.0
28	3.3507263955984856E-4	0.0	0.0	9.158791761569011	0.0
29	3.3507263955984856E-4	0.0	0.0	9.529130796442534	0.0
30	3.3507263955984856E-4	0.0	0.0	9.872915324630938	0.0
31	3.3507263955984856E-4	0.0	0.0	10.187799837657305	0.0
32	3.3507263955984856E-4	0.0	0.0	10.522286100097926	0.0
33	3.3507263955984856E-4	0.0	0.0	10.862384829251171	0.0
34	4.1884079944981073E-4	0.0	0.0	11.26371808328398	0.0
35	4.1884079944981073E-4	0.0	0.0	11.607000002513045	0.0
36	4.1884079944981073E-4	0.0	0.0	11.95111960334101	0.0
37	4.1884079944981073E-4	0.0	0.0	12.313416894865096	0.0
38	4.1884079944981073E-4	0.0	0.0	12.6822481028606	0.0
39	4.1884079944981073E-4	0.0	0.0	13.05736192284785	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATGT	30	2.1655724E-6	45.000004	5
CGTAGGC	35	1.2119744E-7	45.000004	2
GACGGAC	30	2.1655724E-6	45.000004	34
CGTAAAT	35	1.2119744E-7	45.000004	15
CGGTCTA	140	0.0	45.000004	30
ACATCGC	35	1.2119744E-7	45.000004	17
CTATGCG	20	7.033396E-4	45.0	23
TCCGCAT	20	7.033396E-4	45.0	11
CGAGTAC	20	7.033396E-4	45.0	5
TTAGCGA	40	6.8157533E-9	45.0	1
GGCAACG	45	3.8562575E-10	45.0	7
GTGATCG	20	7.033396E-4	45.0	19
CTAAGCG	25	3.8908962E-5	45.0	17
TATGCGC	20	7.033396E-4	45.0	24
CGGATCG	20	7.033396E-4	45.0	5
AGCGATA	20	7.033396E-4	45.0	7
CTCGCGA	20	7.033396E-4	45.0	1
CGTCCAA	20	7.033396E-4	45.0	9
CGTCATG	20	7.033396E-4	45.0	17
GGTTACG	20	7.033396E-4	45.0	26
>>END_MODULE