FastQCFastQC Report
Thu 26 May 2016
SRR1043449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043449_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences606906
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329585.430495002520983No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34250.5643378051955328No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.21947385591837948No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT12680.20892856554392278No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6970.11484480298431718No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.10133364969204456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTA551.8189894E-1245.0000048
ACACAGG551.8189894E-1245.0000041
ACGGGTC551.8189894E-1245.0000044
CTTATGG551.8189894E-1245.0000041
TGATTTG351.2106648E-745.00000428
GTCGTGG351.2106648E-745.0000041
TTGCGTG351.2106648E-745.00000413
ATGACGG351.2106648E-745.0000041
GCGATAG551.8189894E-1245.0000048
AGGGCGT351.2106648E-745.0000045
AGTACGG551.8189894E-1245.0000041
TCACGCA207.030653E-445.024
CAGGTTG207.030653E-445.05
GTCATCA207.030653E-445.020
GTGCAGA253.8886228E-545.01
GGTACTC207.030653E-445.011
TACCCTC207.030653E-445.031
ACAAATG207.030653E-445.026
GTCGCAT253.8886228E-545.09
CGTATTT207.030653E-445.01