##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043449_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	606906
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.588259137329338	31.0	31.0	33.0	30.0	34.0
2	30.691322873723443	31.0	31.0	34.0	26.0	34.0
3	31.556124012614802	31.0	31.0	34.0	28.0	34.0
4	35.14759287270187	37.0	35.0	37.0	32.0	37.0
5	35.45089025318583	37.0	35.0	37.0	33.0	37.0
6	35.8385384227541	37.0	35.0	37.0	35.0	37.0
7	33.99865547547726	37.0	35.0	37.0	32.0	37.0
8	34.90816535015307	37.0	35.0	37.0	32.0	37.0
9	36.84595143234702	39.0	37.0	39.0	32.0	39.0
10	36.780389384847076	39.0	35.0	39.0	32.0	39.0
11	36.286464460723735	38.0	35.0	39.0	32.0	39.0
12	36.02532187851166	38.0	35.0	39.0	32.0	39.0
13	36.12089516333666	38.0	35.0	39.0	32.0	39.0
14	37.48288367556096	40.0	35.0	41.0	33.0	41.0
15	37.69311227768386	40.0	35.0	41.0	33.0	41.0
16	37.64090485182219	40.0	35.0	41.0	33.0	41.0
17	37.66903111849282	39.0	36.0	41.0	33.0	41.0
18	37.41345117695327	39.0	36.0	41.0	33.0	41.0
19	37.13260537875717	38.0	35.0	41.0	33.0	41.0
20	36.836368399719234	38.0	35.0	40.0	33.0	41.0
21	36.759745331237454	38.0	35.0	40.0	32.0	41.0
22	36.693886697445734	37.0	35.0	40.0	32.0	41.0
23	36.61843349711487	37.0	35.0	40.0	32.0	41.0
24	36.462766886470064	37.0	35.0	40.0	32.0	41.0
25	36.38033896517747	36.0	35.0	40.0	32.0	41.0
26	36.12823567405825	36.0	35.0	40.0	31.0	41.0
27	36.10597852056167	36.0	35.0	40.0	31.0	41.0
28	36.02271356684561	36.0	35.0	40.0	31.0	41.0
29	35.99527603945257	36.0	35.0	40.0	31.0	41.0
30	35.82354598570454	36.0	35.0	40.0	31.0	41.0
31	35.45671982152096	36.0	35.0	40.0	30.0	41.0
32	35.32629435200838	36.0	35.0	40.0	29.0	41.0
33	35.234822196518074	36.0	35.0	40.0	29.0	41.0
34	35.08836788563632	36.0	35.0	40.0	28.0	41.0
35	34.85048261180479	36.0	35.0	40.0	25.0	41.0
36	34.67588720493783	36.0	34.0	40.0	25.0	41.0
37	34.62395000214201	35.0	34.0	40.0	25.0	41.0
38	34.55842749948098	35.0	34.0	40.0	24.0	41.0
39	34.34963075006673	35.0	34.0	40.0	23.0	41.0
40	34.343179998220485	35.0	34.0	40.0	23.0	41.0
41	34.27964297601276	35.0	34.0	40.0	23.0	41.0
42	34.170270190111815	35.0	34.0	40.0	23.0	41.0
43	33.97541629181455	35.0	34.0	40.0	22.0	41.0
44	33.78083755968799	35.0	33.0	40.0	21.0	41.0
45	33.619712113572774	35.0	33.0	40.0	20.0	41.0
46	33.61380510326146	35.0	33.0	40.0	21.0	41.0
47	33.621163738700886	35.0	33.0	40.0	22.0	41.0
48	33.58980962455471	35.0	33.0	40.0	20.0	41.0
49	33.463740678128076	35.0	33.0	40.0	20.0	41.0
50	33.32718411088373	35.0	33.0	40.0	20.0	41.0
51	32.81058846015693	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	6.0
14	11.0
15	35.0
16	46.0
17	130.0
18	254.0
19	494.0
20	898.0
21	1485.0
22	2399.0
23	3806.0
24	6068.0
25	9170.0
26	11467.0
27	12719.0
28	12681.0
29	13660.0
30	15382.0
31	18517.0
32	23432.0
33	31773.0
34	59299.0
35	88156.0
36	60447.0
37	51140.0
38	68057.0
39	115289.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.921137705015276	18.622488490804177	24.815375033365957	29.64099877081459
2	30.518894194488112	21.871426547109436	29.09544476409856	18.514234494303896
3	22.318612767051242	21.614055553907853	37.53035890236709	18.536972776673817
4	22.957097145192172	25.280521200976757	33.65068066553964	18.11170098829143
5	18.897489891350556	31.83639641064679	32.79766553634336	16.468448161659303
6	79.8856824615344	2.1891363736723646	16.554128645951764	1.3710525188414682
7	76.72291920000791	6.62260712532089	14.863586782796675	1.790886891874524
8	76.00831100697637	3.670749671283527	17.674565748237782	2.6463735735023213
9	41.704316648706715	24.144430933291154	23.762658467703403	10.388593950298729
10	33.95418730412947	21.085802414212417	34.470576992153646	10.48943328950447
11	33.45905296701631	20.071971606805665	33.2896692403766	13.179306185801426
12	16.721535130646263	36.50697142555849	33.08008159418427	13.691411849610976
13	12.199253261625358	38.352726781412606	34.17267253907524	15.27534741788679
14	9.91619789555549	24.05479596510827	49.98846608865294	16.0405400506833
15	12.123458987058951	19.877872355850823	49.39150379136143	18.607164865728794
16	12.515611972859059	22.104246786157987	35.40268179915835	29.9774594418246
17	15.507673346449039	22.532649207620288	43.10519256688845	18.854484879042225
18	20.8781920099653	25.126625869574532	34.64259704138697	19.3525850790732
19	22.741413003002112	23.95642817833404	35.27795078644798	18.024208032215864
20	15.26101241378401	29.97350495793418	35.92384982188345	18.841632806398355
21	17.788916240735798	24.679110109308525	32.608674160413635	24.92329948954204
22	12.495510013082752	30.046003829258566	33.81166111391221	23.646825043746478
23	12.338154508276405	23.105884601569272	49.50305319110373	15.052907699050595
24	13.12460249198393	25.607754742909115	42.961677755698574	18.305965009408375
25	12.552685259331758	33.08354176758839	35.43514152109223	18.928631451987624
26	11.77167469097356	35.40383519029306	34.62282462193486	18.201665496798515
27	12.524509561612508	28.690769245978785	41.91522245619585	16.869498736212858
28	11.304056970931248	24.912424658843378	41.46457606284993	22.31894230737544
29	13.234009879618919	32.811671000121926	36.58754403482582	17.36677508543333
30	21.024343143748787	30.31045993943049	32.871976879450855	15.793220037369874
31	22.460644646782203	26.74862334529565	34.33579499955512	16.45493700836703
32	20.378938418799617	30.56255828744484	31.788118753151227	17.27038454060431
33	14.93872197671468	28.55944742678438	33.87608624729365	22.62574434920729
34	16.452300685773412	27.45021469552122	35.66911515127549	20.42836946742988
35	25.25283981374381	27.14308311336517	31.491038150883334	16.113038922007693
36	15.35361324488471	32.841823939786394	34.16657604307751	17.637986772251388
37	17.633702748036765	30.79455467568289	34.10132705888556	17.470415517394784
38	17.518363634566146	32.143198452478636	32.091625391741054	18.24681252121416
39	21.126500644251333	26.31774937140183	32.27929860637397	20.276451377972865
40	13.906272140990533	25.880284591023983	35.751994542812234	24.461448725173256
41	17.311906621453733	27.50673086112182	30.469792686182046	24.7115698312424
42	17.585589860703305	26.452366593838256	33.55445489087272	22.40758865458572
43	16.580162331563702	29.581022431809867	34.14169574860028	19.697119488026154
44	13.627810567040036	37.71951504845891	29.302890398183575	19.349783986317487
45	18.088303625273106	32.024234395441795	32.278474755563465	17.60898722372163
46	17.63831631257559	29.651708831351147	31.092788669085493	21.617186186987773
47	16.944469159968758	27.475424530322652	35.99025219721011	19.589854112498475
48	17.729269442055276	24.762978121817877	36.38784259835955	21.1199098377673
49	17.56005048557767	30.822565603240044	32.30615614279641	19.311227768385876
50	15.638006544670837	30.914836894016535	31.910707753754288	21.536448807558337
51	15.84660556989056	26.126945523689006	36.50680665539639	21.519642251024045
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	781.0
1	3404.0
2	6027.0
3	22957.0
4	39887.0
5	23079.0
6	6271.0
7	6436.0
8	6601.0
9	7469.5
10	8338.0
11	9243.0
12	10148.0
13	10010.5
14	9873.0
15	8746.0
16	7619.0
17	6586.0
18	5553.0
19	4989.5
20	4426.0
21	4203.0
22	3980.0
23	3567.5
24	3155.0
25	3278.0
26	4098.5
27	4796.0
28	4975.5
29	5155.0
30	7074.5
31	8994.0
32	10685.5
33	12377.0
34	16210.0
35	20043.0
36	20174.5
37	20306.0
38	21311.5
39	22317.0
40	28537.5
41	34758.0
42	43171.5
43	51585.0
44	53444.5
45	55304.0
46	53672.0
47	52040.0
48	50869.0
49	49698.0
50	43806.5
51	37915.0
52	33238.0
53	28561.0
54	25222.0
55	21883.0
56	19642.0
57	17401.0
58	15715.0
59	14029.0
60	11985.0
61	9941.0
62	8538.0
63	7135.0
64	6187.0
65	5239.0
66	4284.0
67	3329.0
68	2616.0
69	1903.0
70	1847.0
71	1791.0
72	1950.5
73	2110.0
74	1585.0
75	822.5
76	585.0
77	443.5
78	302.0
79	218.0
80	134.0
81	107.0
82	80.0
83	65.5
84	51.0
85	34.0
86	17.0
87	10.5
88	4.0
89	2.0
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	606906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.892918654596752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.11674710034147	24.132543137325555
2	9.524270298009197	5.884650151215802
3	3.551636689790475	3.291612700451353
4	1.8822099886662895	2.3258784028294865
5	1.1504152138139483	1.7769841809682416
6	0.7774384025226935	1.441040479685593
7	0.5145632597540439	1.1127452648358074
8	0.38442753914210637	0.9500870956243122
9	0.2930306280798647	0.8147314220918991
>10	2.604363872896167	18.700126740449246
>50	0.8971597801948408	19.38893056633258
>100	0.2972517344019045	12.91025917410334
>500	0.004323661591300429	0.7616859235458515
>1k	0.001621373096737661	1.0059530226575528
>5k	0.0	0.0
>10k+	5.404576989125536E-4	5.502771737883423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32958	5.430495002520983	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3425	0.5643378051955328	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1332	0.21947385591837948	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1268	0.20892856554392278	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	697	0.11484480298431718	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	615	0.10133364969204456	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10759491585187822	0.0
2	0.0	0.0	0.0	0.3751816591037162	0.0
3	0.0	0.0	0.0	0.6200301199856321	0.0
4	0.0	0.0	0.0	0.7671698747417228	0.0
5	0.0	0.0	0.0	1.6712637541892814	0.0
6	0.0	0.0	0.0	1.874754904383875	0.0
7	0.0	0.0	0.0	2.5297162987348947	0.0
8	0.0	0.0	0.0	3.326544802654777	0.0
9	0.0	0.0	0.0	3.8725931198571115	0.0
10	0.0	0.0	0.0	4.297535367915295	0.0
11	0.0	0.0	0.0	4.750323773368528	0.0
12	0.0	0.0	0.0	5.137039343819306	0.0
13	0.0	0.0	0.0	5.302962897054898	0.0
14	0.0	0.0	0.0	5.430495002520983	0.0
15	0.0	0.0	0.0	5.916566980718596	0.0
16	0.0	0.0	0.0	6.493427318233795	0.0
17	0.0	0.0	0.0	7.213472926614665	0.0
18	0.0	0.0	0.0	7.516979565204497	0.0
19	0.0	0.0	0.0	7.875519437936023	0.0
20	0.0	0.0	0.0	8.253007879309152	0.0
21	0.0019772419452106257	0.0	0.0	8.643677933650352	0.0
22	0.0019772419452106257	0.0	0.0	9.064336157493912	0.0
23	0.0019772419452106257	0.0	0.0	9.366030324300633	0.0
24	0.005437415349329221	0.0	0.0	9.602969817401707	0.0
25	0.005602185511430107	0.0	0.0	9.82870493947992	0.0
26	0.005602185511430107	0.0	0.0	10.082121448791082	0.0
27	0.005602185511430107	0.0	0.0	10.33702088956115	0.0
28	0.005931725835631877	0.0	0.0	10.606090564271897	0.0
29	0.005931725835631877	0.0	0.0	10.948647731279639	0.0
30	0.006096495997732762	0.0	0.0	11.268960926403759	0.0
31	0.006096495997732762	0.0	0.0	11.52040019376971	0.0
32	0.006096495997732762	0.0	0.0	11.814020622633489	0.0
33	0.006096495997732762	0.0	0.0	12.079630123940117	0.0
34	0.006096495997732762	0.0	0.0	12.434050742619121	0.0
35	0.006096495997732762	0.0	0.0	12.691586505982805	0.0
36	0.006096495997732762	0.0	0.0	12.973013942851116	0.0
37	0.006096495997732762	0.0	0.0	13.258560633771951	0.0
38	0.006096495997732762	0.0	0.0	13.547732268259006	0.0
39	0.006096495997732762	0.0	0.0	13.854534310090854	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGTA	55	1.8189894E-12	45.000004	8
ACACAGG	55	1.8189894E-12	45.000004	1
ACGGGTC	55	1.8189894E-12	45.000004	4
CTTATGG	55	1.8189894E-12	45.000004	1
TGATTTG	35	1.2106648E-7	45.000004	28
GTCGTGG	35	1.2106648E-7	45.000004	1
TTGCGTG	35	1.2106648E-7	45.000004	13
ATGACGG	35	1.2106648E-7	45.000004	1
GCGATAG	55	1.8189894E-12	45.000004	8
AGGGCGT	35	1.2106648E-7	45.000004	5
AGTACGG	55	1.8189894E-12	45.000004	1
TCACGCA	20	7.030653E-4	45.0	24
CAGGTTG	20	7.030653E-4	45.0	5
GTCATCA	20	7.030653E-4	45.0	20
GTGCAGA	25	3.8886228E-5	45.0	1
GGTACTC	20	7.030653E-4	45.0	11
TACCCTC	20	7.030653E-4	45.0	31
ACAAATG	20	7.030653E-4	45.0	26
GTCGCAT	25	3.8886228E-5	45.0	9
CGTATTT	20	7.030653E-4	45.0	1
>>END_MODULE