FastQCFastQC Report
Thu 26 May 2016
SRR1043448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043448_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3441304
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1443444.194456519970337No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC324850.9439735635096462No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145590.4230663725146049No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC134610.3911598626567138No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC88430.2569665452398277No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65370.18995706278782695No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA63360.18411625360619113No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC54590.1586317279728847No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG53320.1549412664501596No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC50950.14805434219121588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT422350.043.8599471
CGCACTT100100.041.58341634
GCACTTA100900.041.25371635
CACTTAC100850.041.25185836
ACTTACT100250.041.2069837
CGTTTTA22350.040.8724821
CTTACTG100850.040.85027338
CCGCACT102150.040.77092733
ACCCGCA102050.040.70063831
CCCGCAC102900.040.62682332
ACGGGTA4950.040.04
CACGACG8350.039.8802425
CTTATGA106800.039.71208225
TGACCCG104500.039.63875629
AACGCAA108650.039.63644417
TCAACGC109150.039.5579515
TTATGAC107100.039.53781526
ATCAACG109400.039.4675514
CTTAATC110450.039.43866310
CAACGCA109200.039.43681316