##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043448_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3441304
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52841190432464	31.0	31.0	33.0	30.0	34.0
2	30.547670592310357	31.0	31.0	34.0	26.0	34.0
3	31.542689050429722	31.0	31.0	34.0	28.0	34.0
4	35.18604430181117	37.0	35.0	37.0	33.0	37.0
5	35.52444276936882	37.0	35.0	37.0	33.0	37.0
6	35.751765319192955	37.0	35.0	37.0	35.0	37.0
7	33.65208072288876	37.0	35.0	37.0	32.0	37.0
8	34.71178396328833	37.0	35.0	37.0	31.0	37.0
9	36.727505910550185	39.0	37.0	39.0	32.0	39.0
10	36.820846981260594	39.0	35.0	39.0	32.0	39.0
11	36.779051487459405	39.0	35.0	39.0	33.0	39.0
12	36.820019969174474	39.0	35.0	39.0	33.0	39.0
13	36.86527287330617	39.0	35.0	39.0	33.0	39.0
14	38.15351244760707	40.0	37.0	41.0	33.0	41.0
15	38.217003205761536	40.0	37.0	41.0	33.0	41.0
16	38.081238100441	40.0	37.0	41.0	33.0	41.0
17	38.03372733126745	40.0	37.0	41.0	33.0	41.0
18	37.77921131059622	39.0	36.0	41.0	33.0	41.0
19	37.51102895879004	39.0	36.0	41.0	33.0	41.0
20	37.22391715465998	39.0	35.0	41.0	33.0	41.0
21	37.11843475612733	39.0	35.0	41.0	33.0	41.0
22	37.03663001001946	38.0	35.0	41.0	32.0	41.0
23	36.972158228392495	38.0	35.0	40.0	32.0	41.0
24	36.817372426266324	38.0	35.0	40.0	32.0	41.0
25	36.71991954212705	38.0	35.0	40.0	32.0	41.0
26	36.458054853625256	38.0	35.0	40.0	31.0	41.0
27	36.32218658973459	38.0	35.0	40.0	31.0	41.0
28	36.1352016561164	38.0	35.0	40.0	31.0	41.0
29	36.073504694731994	38.0	35.0	40.0	30.0	41.0
30	35.88960027942896	38.0	35.0	40.0	30.0	41.0
31	35.59751855691912	38.0	35.0	40.0	29.0	41.0
32	35.440957265036744	38.0	35.0	40.0	27.0	41.0
33	35.16232974477117	38.0	34.0	40.0	25.0	41.0
34	34.94314626083601	38.0	34.0	40.0	24.0	41.0
35	34.741591559478614	38.0	34.0	40.0	23.0	41.0
36	34.59444995269235	37.0	34.0	40.0	23.0	41.0
37	34.44472676636531	37.0	34.0	40.0	22.0	41.0
38	34.29714985947187	37.0	34.0	40.0	21.0	41.0
39	34.16529083161499	37.0	34.0	40.0	21.0	41.0
40	34.04342656155922	36.0	33.0	40.0	21.0	41.0
41	33.92377046607914	36.0	33.0	40.0	20.0	41.0
42	33.80183674560573	36.0	33.0	40.0	20.0	41.0
43	33.6200730885734	36.0	33.0	40.0	18.0	41.0
44	33.399483161034304	35.0	33.0	40.0	18.0	41.0
45	33.18225155348089	35.0	33.0	40.0	18.0	41.0
46	33.16804560131857	35.0	33.0	40.0	18.0	41.0
47	33.126653152409666	35.0	33.0	40.0	18.0	41.0
48	33.019843640666444	35.0	33.0	39.0	18.0	41.0
49	32.971276295264815	35.0	33.0	39.0	18.0	41.0
50	32.89690826500652	35.0	33.0	39.0	18.0	41.0
51	32.47649466597546	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	4.0
11	11.0
12	25.0
13	32.0
14	74.0
15	159.0
16	367.0
17	790.0
18	1670.0
19	3162.0
20	5552.0
21	9273.0
22	14382.0
23	22098.0
24	34575.0
25	52796.0
26	72703.0
27	85655.0
28	90071.0
29	90314.0
30	94377.0
31	106998.0
32	127123.0
33	163883.0
34	271896.0
35	323498.0
36	354638.0
37	390440.0
38	499990.0
39	624433.0
40	311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.22621773606749	16.471634008503752	22.155874633569137	32.14627362185962
2	34.9294627850373	19.47668674432715	25.626041756264485	19.967808714371063
3	26.02068866917889	20.449079767436995	34.60862510257739	18.921606460806718
4	22.445328863709804	23.26798213700388	32.39330207386502	21.893386925421293
5	20.61578982850687	32.657126484611645	30.98110483700365	15.745978849877837
6	78.54972998607505	2.2467645985359037	17.726536219991026	1.4769691953980235
7	73.82869981844091	7.706875068288068	16.775762908478878	1.688662204792137
8	72.46828527790629	4.050673814344795	20.14436969241892	3.3366712153300027
9	38.885521302390025	18.113221034817034	28.563213247071463	14.438044415721482
10	25.884780885385304	18.977980439972754	33.146359635765975	21.99087903887596
11	23.134805875912154	15.747170258715881	41.751876614213685	19.36614725115828
12	20.74251504662186	16.44001808616734	42.426272134051516	20.39119473315929
13	18.07329430936645	20.30352447793046	42.252558913714104	19.370622298988987
14	17.59588807033613	19.18034558992754	42.70419003958965	20.51957630014669
15	21.66884994757801	15.409769087531936	42.16314513335643	20.75823583153363
16	22.139950437392336	16.182760953406035	38.32768043741559	23.349608171786045
17	21.782353433465918	19.804876291080358	37.49012002426987	20.922650251183853
18	23.227793882783967	21.614306669797262	35.10201365528881	20.05588579212996
19	23.959347968095816	19.58969623142855	34.84339076117658	21.607565039299057
20	25.32107015247708	20.589840362839205	36.536963895081634	17.55212558960208
21	21.926891666647293	18.047373902450932	36.648578562079955	23.37715586882182
22	22.57222843433768	20.303117655400392	36.484280377438324	20.640373532823606
23	23.16334157052094	19.675564844024244	37.383852167666674	19.77724141778814
24	25.506958989964268	17.74243135741568	35.500786911008156	21.2498227416119
25	21.061231440175003	19.514317828358088	35.59046222013516	23.833988511331754
26	19.914514962932657	19.1626197511176	38.149608404256064	22.773256881693683
27	21.077475282625425	19.952814398262984	38.134991851925896	20.834718467185695
28	21.684745085002664	21.9773667191274	35.74214890634481	20.595739289525135
29	21.6075940980512	19.155500356841475	38.90891359786872	20.327991947238605
30	25.39057868761376	19.869473897104122	34.993508274770264	19.74643914051185
31	24.635777600583964	21.022496123562465	33.79506721870547	20.546659057148105
32	25.071542647787005	19.485694957492857	33.1455169319537	22.29724546276644
33	22.01996684977555	21.550609885090072	34.64474513149666	21.784678133637712
34	22.4487577964632	21.752422918754053	33.33646199231454	22.462357292468205
35	24.336617747226054	24.065209002168945	31.224326592477738	20.373846658127267
36	23.012817234397193	23.549299916543266	30.71789066005212	22.719992189007424
37	23.565049760207177	26.226192164365603	29.45377682413411	20.754981251293113
38	22.59393532219182	23.164794508128313	29.300579082812796	24.94069108686707
39	22.30622461718	21.936858818633866	34.036167685272794	21.720748878913344
40	21.345426036176985	22.04792136934139	34.12145512282553	22.485197471656093
41	20.952261119622094	26.12097042283971	30.325481271053068	22.601287186485123
42	20.65653019901758	21.92157391500431	31.566696810278895	25.855199075699208
43	21.699012932307056	21.30128579166502	34.29124541162303	22.70845586440489
44	23.638364991875175	22.326769154948238	32.62318586210344	21.41167999107315
45	24.110191950493185	22.034554343353566	32.73326041523795	21.121993290915302
46	22.374890448504402	23.45674779095366	31.924497225470343	22.243864535071587
47	21.12649739749816	25.642285598714903	32.34308855015424	20.888128453632692
48	22.119551193384833	24.771743501881847	32.22917824173627	20.87952706299705
49	21.03583409079814	21.989019278738525	34.34363834174488	22.631508288718464
50	20.4569837480211	22.735335210141272	35.211216445858895	21.596464595978734
51	20.16404246762274	21.140445598528927	34.17091311898048	24.52459881486785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1383.0
1	16794.0
2	32205.0
3	126394.0
4	220583.0
5	135731.5
6	50880.0
7	45830.0
8	40780.0
9	39656.0
10	38532.0
11	37261.5
12	35991.0
13	34065.5
14	32140.0
15	29718.5
16	27297.0
17	25480.0
18	23663.0
19	22177.5
20	20692.0
21	20264.0
22	19836.0
23	19449.0
24	19062.0
25	19597.0
26	21657.5
27	23183.0
28	25339.0
29	27495.0
30	32958.5
31	38422.0
32	43531.0
33	48640.0
34	53541.0
35	58442.0
36	61984.0
37	65526.0
38	69135.0
39	72744.0
40	79907.5
41	87071.0
42	93926.0
43	100781.0
44	109221.5
45	117662.0
46	130301.0
47	142940.0
48	170945.5
49	198951.0
50	202509.0
51	206067.0
52	191259.5
53	176452.0
54	177325.0
55	178198.0
56	185039.0
57	191880.0
58	195138.0
59	198396.0
60	198093.0
61	197790.0
62	193217.5
63	188645.0
64	174378.0
65	160111.0
66	136924.0
67	113737.0
68	96821.5
69	79906.0
70	70067.0
71	60228.0
72	55959.5
73	51691.0
74	43690.0
75	27415.0
76	19141.0
77	14074.0
78	9007.0
79	6914.0
80	4821.0
81	3645.5
82	2470.0
83	1790.5
84	1111.0
85	854.5
86	598.0
87	400.0
88	202.0
89	139.5
90	77.0
91	53.5
92	30.0
93	23.0
94	16.0
95	12.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3441304.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.654413444783486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.75379100671651	20.191728655027426
2	8.150094984811524	4.344720026788455
3	2.706923797014151	2.16454398147415
4	1.563263511711022	1.6667148785715884
5	1.056979442500362	1.40865835315207
6	0.8890815473715282	1.421876828986114
7	0.7310044706846531	1.36391467741297
8	0.6199659398940941	1.3219862786896777
9	0.5427277997050033	1.301948204518332
>10	7.4336758183714045	43.79356402123938
>50	0.46855209483132043	8.005671171328311
>100	0.07438297559476578	3.5237103165034647
>500	0.00656297367762599	1.2567191621317229
>1k	0.0018422382252971873	0.7934556941008725
>5k	6.908393344864452E-4	1.1540001937642534
>10k+	4.6055955632429683E-4	6.286787556311195
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	144344	4.194456519970337	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	32485	0.9439735635096462	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14559	0.4230663725146049	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13461	0.3911598626567138	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8843	0.2569665452398277	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6537	0.18995706278782695	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	6336	0.18411625360619113	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5459	0.1586317279728847	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	5332	0.1549412664501596	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5095	0.14805434219121588	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.036614027705776646	0.0
2	0.0	0.0	0.0	0.10908655556149646	0.0
3	0.0	0.0	0.0	0.17844979693743998	0.0
4	0.0	0.0	0.0	0.2274137943058794	0.0
5	0.0	0.0	0.0	0.49152879257397775	0.0
6	0.0	0.0	0.0	0.6385370196878858	0.0
7	0.0	0.0	0.0	0.96960338290369	0.0
8	0.0	0.0	0.0	1.308689961712188	0.0
9	0.0	0.0	0.0	1.670645778460723	0.0
10	0.0	0.0	0.0	1.8731562221762448	0.0
11	0.0	0.0	0.0	2.0585510608769235	0.0
12	0.0	0.0	0.0	2.2116035084374994	0.0
13	0.0	0.0	0.0	2.29235778065524	0.0
14	0.0	0.0	0.0	2.349923168659322	0.0
15	0.0	0.0	0.0	2.48955047272778	0.0
16	0.0	0.0	0.0	2.638592812492009	0.0
17	0.0	0.0	0.0	2.852319934536443	0.0
18	0.0	0.0	0.0	2.960854373807138	0.0
19	0.0	0.0	0.0	3.1042011981504687	0.0
20	0.0	0.0	0.0	3.252749539128191	0.0
21	0.0	0.0	0.0	3.4134734972556915	0.0
22	0.0	0.0	0.0	3.5775973293844427	0.0
23	0.0	0.0	0.0	3.724082498959697	0.0
24	0.0	0.0	0.0	3.8558348811961975	0.0
25	0.0	0.0	0.0	3.9861343258253266	0.0
26	0.0	0.0	0.0	4.126139393671701	0.0
27	0.0	0.0	0.0	4.2789303124629505	0.0
28	0.0	0.0	0.0	4.434510871460353	0.0
29	0.0	0.0	0.0	4.590992251774328	0.0
30	0.0	0.0	0.0	4.748694099678494	0.0
31	0.0	0.0	0.0	4.907500180164264	0.0
32	0.0	0.0	0.0	5.097718771721417	0.0
33	0.0	0.0	0.0	5.276546332436774	0.0
34	0.0	0.0	0.0	5.4941963860211125	0.0
35	0.0	0.0	0.0	5.68575168017705	0.0
36	0.0	0.0	0.0	5.890180001534302	0.0
37	0.0	0.0	0.0	6.128374592886883	0.0
38	0.0	0.0	0.0	6.380313974005203	0.0
39	0.0	0.0	0.0	6.674010781959397	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	42235	0.0	43.859947	1
CGCACTT	10010	0.0	41.583416	34
GCACTTA	10090	0.0	41.253716	35
CACTTAC	10085	0.0	41.251858	36
ACTTACT	10025	0.0	41.20698	37
CGTTTTA	2235	0.0	40.872482	1
CTTACTG	10085	0.0	40.850273	38
CCGCACT	10215	0.0	40.770927	33
ACCCGCA	10205	0.0	40.700638	31
CCCGCAC	10290	0.0	40.626823	32
ACGGGTA	495	0.0	40.0	4
CACGACG	835	0.0	39.88024	25
CTTATGA	10680	0.0	39.712082	25
TGACCCG	10450	0.0	39.638756	29
AACGCAA	10865	0.0	39.636444	17
TCAACGC	10915	0.0	39.55795	15
TTATGAC	10710	0.0	39.537815	26
ATCAACG	10940	0.0	39.46755	14
CTTAATC	11045	0.0	39.438663	10
CAACGCA	10920	0.0	39.436813	16
>>END_MODULE