FastQCFastQC Report
Thu 26 May 2016
SRR1043447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043447_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3148973
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1016793.2289575045578354No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC236250.7502446035580489No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108490.3445250245079904No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC100500.3191516726246938No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC87410.27758256422014416No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC49510.15722586379749842No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA48430.15379617418123306No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC47920.15217659852910775No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.13636191863188413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT290500.043.85371
GCGATCG900.042.58
ATAACGG1200.041.2499961
CATGCGG4700.041.170211
TAGTAGG7150.040.5944061
CGCACTT82200.040.59306734
CACTTAC82200.040.59306736
CTTACTG81700.040.48347538
CCGCACT82150.040.4260533
GCACTTA82600.040.3147735
TTCACGG2400.040.3124961
ACCCGCA82600.040.2058131
ACTTACT82900.040.08745637
CATATGC20700.040.032
TAGCATA20750.039.90361429
CCCGCAC83750.039.84179332
GTAGCAT21600.039.79166428
GTTGATC22300.039.34977315
ATAGGGC7450.039.2617453
GCCAGTA22200.039.22297324