##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043447_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3148973
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.486875244722643	31.0	31.0	33.0	30.0	34.0
2	30.527302711074373	31.0	31.0	34.0	26.0	34.0
3	31.497218616990367	31.0	31.0	34.0	28.0	34.0
4	35.12703633851417	37.0	35.0	37.0	32.0	37.0
5	35.448976539335206	37.0	35.0	37.0	33.0	37.0
6	35.717607613656895	37.0	35.0	37.0	35.0	37.0
7	33.670165161784496	37.0	35.0	37.0	31.0	37.0
8	34.68347076967633	37.0	35.0	37.0	31.0	37.0
9	36.73576369184493	39.0	37.0	39.0	32.0	39.0
10	36.652500990005315	39.0	35.0	39.0	32.0	39.0
11	36.2163714963577	38.0	35.0	39.0	32.0	39.0
12	36.159245252341	38.0	35.0	39.0	32.0	39.0
13	36.140994857688526	38.0	35.0	39.0	32.0	39.0
14	37.24606975035988	39.0	35.0	41.0	32.0	41.0
15	37.32554836132288	39.0	35.0	41.0	32.0	41.0
16	37.04597467174219	39.0	35.0	41.0	31.0	41.0
17	36.95927942221162	39.0	35.0	41.0	31.0	41.0
18	36.89774221627178	39.0	35.0	40.0	31.0	41.0
19	36.774328011069	38.0	35.0	40.0	31.0	41.0
20	36.55321973227462	38.0	35.0	40.0	31.0	41.0
21	36.484914605492015	38.0	35.0	40.0	31.0	41.0
22	36.45021757887413	38.0	35.0	40.0	31.0	41.0
23	36.420547905618754	37.0	35.0	40.0	31.0	41.0
24	36.30886482672287	37.0	35.0	40.0	31.0	41.0
25	36.18766181863103	37.0	35.0	40.0	31.0	41.0
26	35.892765355561956	37.0	35.0	40.0	30.0	41.0
27	35.79318209460672	36.0	35.0	40.0	30.0	41.0
28	35.62974817503993	36.0	34.0	40.0	30.0	41.0
29	35.453317002082905	36.0	34.0	40.0	29.0	41.0
30	35.227338246469564	36.0	34.0	40.0	29.0	41.0
31	35.110131779472226	36.0	34.0	40.0	28.0	41.0
32	35.035414720926475	36.0	34.0	40.0	27.0	41.0
33	34.65659534076666	36.0	33.0	40.0	24.0	41.0
34	34.53015125883899	36.0	33.0	40.0	24.0	41.0
35	34.51027938315127	36.0	34.0	40.0	24.0	41.0
36	34.45912746790779	35.0	34.0	40.0	24.0	41.0
37	34.35747178524554	35.0	34.0	40.0	23.0	41.0
38	34.26320962421717	35.0	34.0	40.0	23.0	41.0
39	34.171764254568075	35.0	34.0	40.0	23.0	41.0
40	34.1075801539105	35.0	33.0	40.0	23.0	41.0
41	34.01169682941073	35.0	33.0	40.0	23.0	41.0
42	33.87267436081542	35.0	33.0	40.0	23.0	41.0
43	33.67703756113501	35.0	33.0	40.0	23.0	41.0
44	33.44199743852996	35.0	33.0	39.0	21.0	41.0
45	33.19360407345506	35.0	33.0	39.0	20.0	41.0
46	33.19185366149535	35.0	33.0	39.0	20.0	41.0
47	33.184908857586265	35.0	33.0	39.0	22.0	41.0
48	33.09784047052801	35.0	33.0	39.0	21.0	41.0
49	33.05835934445929	35.0	33.0	39.0	21.0	41.0
50	33.009038184830416	35.0	33.0	38.0	21.0	41.0
51	32.58022472723647	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	6.0
11	13.0
12	14.0
13	40.0
14	98.0
15	194.0
16	449.0
17	1011.0
18	1978.0
19	3561.0
20	5971.0
21	9709.0
22	14106.0
23	21030.0
24	30946.0
25	44144.0
26	59110.0
27	71486.0
28	80454.0
29	88796.0
30	99471.0
31	115887.0
32	138177.0
33	182257.0
34	353121.0
35	335566.0
36	296598.0
37	324113.0
38	398064.0
39	472328.0
40	271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.046200142078067	17.568648572090012	24.486491310023933	28.898659975807984
2	31.49468096423818	20.648890924120337	27.488422415816206	20.36800569582527
3	26.34601820974648	21.880752867680986	32.43241526681874	19.3408136557538
4	23.625512190799984	24.296238805477213	29.872088455505967	22.20616054821683
5	21.88040354744229	33.14445693881783	27.802937656181875	17.172201857558004
6	82.65796499366618	2.080837149127668	13.84765763313944	1.4135402240667037
7	78.49476638891474	7.210731879885918	12.856223282956062	1.4382784482432844
8	78.17059720740698	3.176178392129751	15.708486544660753	2.944737855802511
9	49.804809377533566	15.800643574905216	20.743461439650325	13.651085607910895
10	35.54657343838769	16.14843950710279	27.646410432861764	20.658576621647757
11	32.158198879444186	13.415294446792652	35.575535261813926	18.85097141194923
12	24.90164253551872	15.892006695516283	36.400121563443065	22.806229205521927
13	18.924487444001585	18.66005202331046	41.96399905620023	20.45146147648773
14	22.33172529583455	16.087943593038112	42.14088212252058	19.43944898860676
15	29.22892638330021	13.51300884447088	38.27228750452926	18.98577726769966
16	26.733573136384464	14.763067196828935	35.86985344110604	22.633506225680563
17	26.724300271866415	18.15884099355568	35.787667915857014	19.3291908187209
18	27.112617351752462	23.63596004157546	30.51102692846207	18.740395678210007
19	28.714155377007046	22.04826780032728	29.151154995612856	20.08642182705282
20	28.097287591859317	24.240284054515552	31.572103031686837	16.090325321938295
21	23.19978608898838	19.349641930877144	35.65727619766826	21.79329578246622
22	27.220207985270118	18.636901618400668	34.069965033044106	20.07292536328511
23	30.067421981706417	17.58036667827892	32.86106295608124	19.491148383933428
24	28.463915060561014	15.626745608806427	30.96022099903683	24.94911833159573
25	20.851560175333354	17.578842371782798	34.56758759125594	27.0020098616279
26	18.621817335366163	18.456938182702743	36.273223047641245	26.64802143428985
27	20.143869128125267	23.027634724083057	33.754401831962355	23.07409431582932
28	25.659349889630683	23.131224053048406	30.817507803337783	20.391918253983125
29	24.693892262651982	18.924932033396285	36.047054071279746	20.334121632671987
30	30.79750128057624	18.35239616217732	32.343211580410504	18.50689097683594
31	32.96646239901072	18.76535619708394	29.39237649862352	18.875804905281818
32	31.83272768613767	17.97935390363779	28.928352196096952	21.259566214127588
33	24.60163996325151	20.38871085906421	34.392832202753084	20.616816974931194
34	21.81654145653202	25.402948834429512	32.19233699368016	20.588172715358308
35	25.21148958724003	29.32778401085052	26.84811841829066	18.612607983618787
36	26.540303775230846	26.15335221991424	25.97872385695273	21.32762014790219
37	25.118824454830197	30.211310163662887	24.807230801915416	19.862634579591504
38	25.194023575305348	24.84651345057579	25.68431040850461	24.27515256561425
39	24.104906583829077	20.196267163929317	29.89803342232531	25.80079282991629
40	19.759267545323507	22.953801128177346	30.394989096445098	26.89194223005405
41	19.840087546003094	25.264014648585427	26.75132495578717	28.144572849624304
42	19.06764522909533	19.516077146422024	28.961569375158184	32.45470824932446
43	20.350095094495888	18.636806349244658	31.57137263482411	29.441725921435335
44	24.99465698816725	20.983634981944906	29.287548670630077	24.734159359257767
45	25.149151802825877	23.531799097674067	29.59618897970862	21.722860119791438
46	21.068869120186168	23.584419428175472	29.48542270765738	25.861288743980975
47	20.128467281237405	23.20785221086367	32.9381357032912	23.725544804607722
48	23.90299948586412	21.914351123366252	32.24527488803493	21.937374502734702
49	21.86169903647951	20.467339669155628	32.996503939538385	24.67445735482648
50	19.724526059766152	20.872741684352327	35.41040205806782	23.9923301978137
51	19.097115154686943	18.963261990496587	34.1822873679768	27.75733548683968
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1670.0
1	14663.0
2	27656.0
3	99493.5
4	171331.0
5	103306.0
6	35281.0
7	29745.0
8	24209.0
9	23626.5
10	23044.0
11	22593.0
12	22142.0
13	21303.0
14	20464.0
15	18788.0
16	17112.0
17	15908.5
18	14705.0
19	13932.5
20	13160.0
21	12593.5
22	12027.0
23	11919.0
24	11811.0
25	11823.0
26	14172.0
27	16509.0
28	17335.5
29	18162.0
30	20346.0
31	22530.0
32	26563.5
33	30597.0
34	33406.0
35	36215.0
36	38035.0
37	39855.0
38	44819.0
39	49783.0
40	58397.0
41	67011.0
42	75976.5
43	84942.0
44	91594.5
45	98247.0
46	105345.5
47	112444.0
48	134698.0
49	156952.0
50	162247.5
51	167543.0
52	164440.5
53	161338.0
54	169994.0
55	178650.0
56	189637.0
57	200624.0
58	212246.0
59	223868.0
60	237314.0
61	250760.0
62	247614.5
63	244469.0
64	226317.0
65	208165.0
66	171225.0
67	134285.0
68	105162.0
69	76039.0
70	65068.0
71	54097.0
72	50644.0
73	47191.0
74	38385.0
75	22562.0
76	15545.0
77	12187.5
78	8830.0
79	6440.0
80	4050.0
81	3043.0
82	2036.0
83	1715.5
84	1395.0
85	961.5
86	528.0
87	342.5
88	157.0
89	112.5
90	68.0
91	50.0
92	32.0
93	24.5
94	17.0
95	13.5
96	10.0
97	5.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3148973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.406705461822355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.12682537184422	18.82411710056009
2	8.586797421256646	4.191508710418933
3	2.7920886666247355	2.0443705712880664
4	1.4050025792590892	1.3716593650030922
5	0.9072870732585376	1.1071944183169982
6	0.6528892583323043	0.9560925496382519
7	0.5258534918174147	0.8984045903600918
8	0.42797453411002884	0.8356358719347293
9	0.38915485506838626	0.8548189134023042
>10	5.752479559707595	33.42900699514525
>50	1.2214951638605136	19.997856675388903
>100	0.20282745671717128	7.632252915809181
>500	0.00542773369538971	0.9416119844517978
>1k	0.0032009711536913677	1.6524150718814838
>5k	1.3917265885614641E-4	0.296909575992683
>10k+	5.566906354245857E-4	4.966144690408218
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101679	3.2289575045578354	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	23625	0.7502446035580489	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10849	0.3445250245079904	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10050	0.3191516726246938	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8741	0.27758256422014416	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4951	0.15722586379749842	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4843	0.15379617418123306	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4792	0.15217659852910775	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4294	0.13636191863188413	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03289961520787889	0.0
2	0.0	0.0	0.0	0.1041927002867284	0.0
3	0.0	0.0	0.0	0.16643521554487764	0.0
4	0.0	0.0	0.0	0.20765500371073362	0.0
5	0.0	0.0	0.0	0.4642783536092561	0.0
6	0.0	0.0	0.0	0.5724723584482941	0.0
7	0.0	0.0	0.0	0.8525001643392941	0.0
8	0.0	0.0	0.0	1.2105216526149953	0.0
9	0.0	0.0	0.0	1.5383110620510243	0.0
10	0.0	0.0	0.0	1.7442194645682894	0.0
11	0.0	0.0	0.0	1.928978114451918	0.0
12	0.0	0.0	0.0	2.080710123586325	0.0
13	0.0	0.0	0.0	2.1602598688524797	0.0
14	0.0	0.0	0.0	2.2206605137611533	0.0
15	0.0	0.0	0.0	2.369153371591309	0.0
16	0.0	0.0	0.0	2.532222410290593	0.0
17	0.0	0.0	0.0	2.7475624592525882	0.0
18	0.0	0.0	0.0	2.8805264446535426	0.0
19	0.0	0.0	0.0	3.0358786817162295	0.0
20	0.0	0.0	0.0	3.205489535794686	0.0
21	0.0	0.0	0.0	3.382499627656382	0.0
22	0.0	0.0	0.0	3.5501733422293555	0.0
23	0.0	0.0	0.0	3.7145443927274067	0.0
24	9.526915600737129E-5	0.0	0.0	3.8624338792361828	0.0
25	9.526915600737129E-5	0.0	0.0	4.0038768195217935	0.0
26	9.526915600737129E-5	0.0	0.0	4.151194691094525	0.0
27	9.526915600737129E-5	0.0	0.0	4.313406307389743	0.0
28	1.9053831201474257E-4	0.0	0.0	4.473744296950149	0.0
29	1.9053831201474257E-4	0.0	0.0	4.647197673654236	0.0
30	1.9053831201474257E-4	0.0	0.0	4.815951105328626	0.0
31	1.9053831201474257E-4	0.0	0.0	5.000169896661546	0.0
32	1.9053831201474257E-4	0.0	0.0	5.223861874966854	0.0
33	1.9053831201474257E-4	0.0	0.0	5.428277727373337	0.0
34	1.9053831201474257E-4	0.0	0.0	5.672706625302916	0.0
35	1.9053831201474257E-4	0.0	0.0	5.883124434537864	0.0
36	1.9053831201474257E-4	0.0	0.0	6.126314833439347	0.0
37	1.9053831201474257E-4	0.0	0.0	6.487384934707284	0.0
38	1.9053831201474257E-4	0.0	0.0	6.8863086472954835	0.0
39	1.9053831201474257E-4	0.0	0.0	7.338074985082438	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	29050	0.0	43.8537	1
GCGATCG	90	0.0	42.5	8
ATAACGG	120	0.0	41.249996	1
CATGCGG	470	0.0	41.17021	1
TAGTAGG	715	0.0	40.594406	1
CGCACTT	8220	0.0	40.593067	34
CACTTAC	8220	0.0	40.593067	36
CTTACTG	8170	0.0	40.483475	38
CCGCACT	8215	0.0	40.42605	33
GCACTTA	8260	0.0	40.31477	35
TTCACGG	240	0.0	40.312496	1
ACCCGCA	8260	0.0	40.20581	31
ACTTACT	8290	0.0	40.087456	37
CATATGC	2070	0.0	40.0	32
TAGCATA	2075	0.0	39.903614	29
CCCGCAC	8375	0.0	39.841793	32
GTAGCAT	2160	0.0	39.791664	28
GTTGATC	2230	0.0	39.349773	15
ATAGGGC	745	0.0	39.261745	3
GCCAGTA	2220	0.0	39.222973	24
>>END_MODULE