FastQCFastQC Report
Thu 26 May 2016
SRR1043446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043446_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences556691
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470298.447954071468732No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47750.8577469368105465No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.34489510338769624No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.1552027965244633No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC5970.1072408212096118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATG253.8882034E-545.00000416
AGGCGTG253.8882034E-545.0000042
ATACTCC302.163477E-645.00000437
CGGATCA253.8882034E-545.00000429
AATCTGC253.8882034E-545.00000444
CTCATGG253.8882034E-545.0000041
GCCAGCG302.163477E-645.00000439
GGCGCAG253.8882034E-545.0000047
GTCCACT302.163477E-645.00000427
CATCCGG302.163477E-645.0000041
CGTCCCC302.163477E-645.00000438
CTGTGTT302.163477E-645.00000411
AATGTAG302.163477E-645.00000415
TCGCCGT207.030149E-445.024
TGTCAGT207.030149E-445.020
CTGTCGG351.2104101E-745.01
ACCGGAC207.030149E-445.033
ACTGCGG551.8189894E-1245.01
CGTATTT207.030149E-445.01
GATAATC207.030149E-445.08