FastQCFastQC Report
Thu 26 May 2016
SRR1043445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043445_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2064238
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT744533.6068030915039837No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80540.3901681879705732No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC73350.355336933047449No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33720.16335325674655732No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC33020.15996217490425038No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC32120.15560221253557002No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA26720.1294424383234879No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAA207.034594E-445.029
TGCGTAC207.034594E-445.024
CGTAATC253.89189E-544.99999633
CGTTTTT234450.044.145871
TCACGAC6550.043.62595724
CGGTCTA6100.043.52458630
ACGGTCT6200.043.18548229
CACGACG6450.042.5581425
CCGCACT28950.041.89119333
CGACGGT6400.041.83593827
CTAGCGG5400.041.6666681
CCCGCAC29450.041.5619732
ACGGGAT7500.041.1000024
ACCCGCA29400.041.0969431
CACTTAC29650.041.05396336
CGTTTTA12450.041.0240971
CGCACTT29600.040.8952734
ATCAACG31600.040.65664714
GACGGTC6600.040.5681828
CAACGCA31650.040.37914716
Produced by FastQC (version 0.11.5)