##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043445_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2064238
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.533170109260656	31.0	31.0	33.0	30.0	34.0
2	30.567944684672987	31.0	31.0	34.0	26.0	34.0
3	31.530682508509194	31.0	31.0	34.0	28.0	34.0
4	35.13871801604272	37.0	35.0	37.0	32.0	37.0
5	35.45469853766862	37.0	35.0	37.0	33.0	37.0
6	35.817996277561015	37.0	35.0	37.0	35.0	37.0
7	33.744039689221886	37.0	35.0	37.0	32.0	37.0
8	34.76219505696533	37.0	35.0	37.0	31.0	37.0
9	36.74804746351923	39.0	37.0	39.0	32.0	39.0
10	36.72167598891213	39.0	35.0	39.0	32.0	39.0
11	36.54622916543538	39.0	35.0	39.0	32.0	39.0
12	36.555799282834634	39.0	35.0	39.0	32.0	39.0
13	36.585093385549534	39.0	35.0	39.0	32.0	39.0
14	37.87708297202164	40.0	36.0	41.0	33.0	41.0
15	37.97456204178007	40.0	36.0	41.0	33.0	41.0
16	37.83937801745729	40.0	36.0	41.0	33.0	41.0
17	37.78751045179868	40.0	36.0	41.0	33.0	41.0
18	37.57031069091839	39.0	36.0	41.0	33.0	41.0
19	37.35227236394253	39.0	35.0	41.0	33.0	41.0
20	37.08131668925773	38.0	35.0	41.0	32.0	41.0
21	36.97537299478064	38.0	35.0	41.0	32.0	41.0
22	36.90211739150234	38.0	35.0	40.0	32.0	41.0
23	36.84092241301633	38.0	35.0	40.0	32.0	41.0
24	36.70916483467507	38.0	35.0	40.0	32.0	41.0
25	36.608697737373305	38.0	35.0	40.0	32.0	41.0
26	36.358334164955785	38.0	35.0	40.0	31.0	41.0
27	36.22927540332074	38.0	35.0	40.0	31.0	41.0
28	36.068510026460125	37.0	35.0	40.0	30.0	41.0
29	35.97339502518605	37.0	35.0	40.0	30.0	41.0
30	35.821431443467276	37.0	35.0	40.0	30.0	41.0
31	35.469666288480305	37.0	34.0	40.0	29.0	41.0
32	35.32774127789528	38.0	34.0	40.0	27.0	41.0
33	35.07376426555465	38.0	34.0	40.0	25.0	41.0
34	34.89314410450733	38.0	34.0	40.0	24.0	41.0
35	34.70775172242735	38.0	34.0	40.0	23.0	41.0
36	34.55677107000259	37.0	34.0	40.0	22.0	41.0
37	34.42821612624126	37.0	34.0	40.0	22.0	41.0
38	34.323374533362916	37.0	34.0	40.0	21.0	41.0
39	34.19425812333655	37.0	34.0	40.0	21.0	41.0
40	34.11364241913965	37.0	34.0	40.0	21.0	41.0
41	34.01387776021951	37.0	33.0	40.0	20.0	41.0
42	33.91562746156209	36.0	33.0	40.0	20.0	41.0
43	33.75648205294157	36.0	33.0	40.0	18.0	41.0
44	33.555880184358585	36.0	33.0	40.0	18.0	41.0
45	33.35670450791042	35.0	33.0	40.0	18.0	41.0
46	33.365795029449124	35.0	33.0	40.0	18.0	41.0
47	33.33283662058348	35.0	33.0	40.0	18.0	41.0
48	33.23726527658148	35.0	33.0	40.0	18.0	41.0
49	33.181270279880515	35.0	33.0	40.0	18.0	41.0
50	33.09488828323091	35.0	33.0	40.0	18.0	41.0
51	32.676377433222335	35.0	32.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	3.0
12	11.0
13	31.0
14	45.0
15	101.0
16	218.0
17	536.0
18	1157.0
19	2160.0
20	3836.0
21	6031.0
22	9465.0
23	14726.0
24	22990.0
25	34592.0
26	44670.0
27	50183.0
28	51815.0
29	52691.0
30	56831.0
31	64558.0
32	77705.0
33	100659.0
34	169160.0
35	199196.0
36	197596.0
37	212375.0
38	283282.0
39	407411.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.470814896344315	17.321064722188044	24.69502063231081	30.51309974915683
2	31.594467304642198	20.42124018645137	27.720834516174975	20.26345799273146
3	23.601590514272093	21.34027180974287	36.021863757958144	19.036273918026893
4	22.74364680816844	24.523964775379582	32.78139439347595	19.950994022976033
5	20.39241599079176	32.48312452343189	31.207593310461295	15.916866175315056
6	80.42178275954613	2.1789638597874856	16.193094013384115	1.206159367282261
7	76.42437548383472	7.322605242224976	14.946386996073127	1.306632277867184
8	75.50665184925381	4.103548137375633	17.773628816057062	2.6161711973134882
9	39.16549351382931	23.067543568135072	24.1537555262523	13.61320739178331
10	28.14161932877895	22.67461407066433	34.168153090874206	15.015613509682508
11	26.234571788718164	19.24201569780229	38.52588703434391	15.997525479135643
12	22.462090127204323	21.528040855754035	37.263435708479356	18.74643330856229
13	17.92748704364516	24.20840038793976	39.27250636796726	18.591606200447817
14	18.26533568319157	21.771181423847445	40.58873056304554	19.374752329915445
15	21.932209367330703	19.303539611227	39.9262100591114	18.838040962330894
16	22.05443364573271	19.59294422445474	37.273027625690446	21.079594504122102
17	22.31317319030073	20.922684302875926	37.42325255130465	19.340889955518694
18	23.161476535166972	24.194787616544218	34.646392518692124	17.997343329596685
19	24.031676579929254	23.318483624465784	34.347686652411205	18.30215314319376
20	22.468194074520476	25.273539194608375	36.09113871559384	16.167128015277306
21	20.622573559831764	21.343469115479902	36.950341966381785	21.083615358306552
22	20.26520197767893	23.382478183232745	36.49947341343391	19.852846425654405
23	20.569333574907546	22.489025005837505	38.64099004087707	18.30065137837788
24	21.556719719334687	21.822483647718915	36.91512315924811	19.705673473698283
25	18.197465602319113	24.663919567414222	36.391394790716966	20.747220039549703
26	17.728382095475425	24.241875210125965	36.513667513145286	21.516075181253324
27	19.274134087251568	24.32723358449946	37.92828152567679	18.470350802572185
28	19.54261088110964	24.106183492407368	36.50063607006556	19.850569556417426
29	19.8450469374171	23.458922856763607	38.44605127897074	18.249978926848552
30	23.62615163561566	23.704243406041357	34.581719743556704	18.08788521478628
31	22.749217871195086	23.703371413567623	35.17569195024992	18.37171876498737
32	23.148154427929338	23.389696343154228	33.53479589078391	19.927353338132523
33	19.516402662871236	25.381278709141096	35.04416641879473	20.05815220919293
34	19.44097531389307	25.271020105239806	34.47945440399799	20.80855017686914
35	22.386081449910332	27.007302452527277	31.62755457461785	18.979061522944544
36	20.546177330327218	27.204566527696905	31.577124343220113	20.672131798755764
37	21.10071609959704	29.012255369778096	30.78240009146232	19.10462843916254
38	21.368078680849788	27.08641154750567	30.260270375799692	21.28523939584486
39	21.347296193559075	25.147342506048236	32.78037706892326	20.724984231469435
40	19.237413515302016	25.84130318306319	32.89896804535136	22.02231525628343
41	19.175695825772028	27.584997466377427	30.83166766622841	22.407639041622136
42	19.100074700688584	25.00409352022393	31.60604542693236	24.28978635215513
43	19.589359366507157	24.750634374524644	33.15339607157702	22.506610187391182
44	19.89547716881484	27.760316397624692	32.13839683214823	20.205809601412238
45	20.97248476193152	27.36535225104857	32.56082874164704	19.10133424537287
46	19.51853420002926	27.105546937901543	31.276819824070675	22.099099037998528
47	19.103029786294023	27.17966629816911	33.38524918153817	20.332054733998696
48	21.072231012121666	26.203131615637343	32.480944542247556	20.24369282999344
49	19.964461462292622	25.543178645098095	32.755379951342825	21.736979941266462
50	18.63782180155583	26.59523756466066	34.03003917183968	20.736901461943827
51	17.929957688987415	24.689352681231526	34.3201704454622	23.060519184318863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	688.0
1	8964.5
2	17241.0
3	64767.5
4	112294.0
5	70040.5
6	27787.0
7	26052.5
8	24318.0
9	24968.0
10	25618.0
11	26353.0
12	27088.0
13	26273.5
14	25459.0
15	23499.5
16	21540.0
17	19484.0
18	17428.0
19	15968.5
20	14509.0
21	14029.5
22	13550.0
23	13144.5
24	12739.0
25	13124.5
26	15299.0
27	17088.0
28	18655.5
29	20223.0
30	24389.5
31	28556.0
32	31087.5
33	33619.0
34	38752.0
35	43885.0
36	49572.0
37	55259.0
38	60968.0
39	66677.0
40	71835.0
41	76993.0
42	87959.0
43	98925.0
44	105125.5
45	111326.0
46	117290.0
47	123254.0
48	131156.0
49	139058.0
50	138038.5
51	137019.0
52	131182.5
53	125346.0
54	119248.5
55	113151.0
56	108955.5
57	104760.0
58	99520.5
59	94281.0
60	88028.0
61	81775.0
62	75965.5
63	70156.0
64	61024.0
65	51892.0
66	42854.0
67	33816.0
68	28226.5
69	22637.0
70	20222.0
71	17807.0
72	18464.0
73	19121.0
74	15941.5
75	9319.5
76	5877.0
77	4084.0
78	2291.0
79	1781.0
80	1271.0
81	999.0
82	727.0
83	658.0
84	589.0
85	405.0
86	221.0
87	134.5
88	48.0
89	37.5
90	27.0
91	23.0
92	19.0
93	12.5
94	6.0
95	5.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2064238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.47787600617505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7656508980089	23.70131865609011
2	9.121971681292306	5.560366436685342
3	3.2333363117962097	2.9563566959156464
4	1.6790777883627852	2.046988985537744
5	1.0295513078058809	1.5689268550651503
6	0.7384783188701745	1.3504350381464267
7	0.5629663428742036	1.2010612875638866
8	0.4567865532256621	1.1137507144399847
9	0.3615124632404861	0.9916318826397408
>10	4.045094134050632	28.55024421824658
>50	0.8373546242332467	17.23574204300073
>100	0.1630717299713328	7.517102921167555
>500	0.003321191140740182	0.7019298656703956
>1k	0.0013284764562960729	0.9571126750084974
>5k	3.321191140740182E-4	0.7788592330234132
>10k+	1.660595570370091E-4	3.7681724917988295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74453	3.6068030915039837	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8054	0.3901681879705732	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7335	0.355336933047449	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3372	0.16335325674655732	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3302	0.15996217490425038	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3212	0.15560221253557002	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2672	0.1294424383234879	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04229163497619945	0.0
2	0.0	0.0	0.0	0.1403423442451888	0.0
3	0.0	0.0	0.0	0.23156244580324556	0.0
4	0.0	0.0	0.0	0.2880966245171342	0.0
5	0.0	0.0	0.0	0.6115089442205792	0.0
6	0.0	0.0	0.0	0.7160511530162704	0.0
7	0.0	0.0	0.0	1.0173729967184018	0.0
8	0.0	0.0	0.0	1.3683015233708515	0.0
9	0.0	0.0	0.0	1.6780526276524315	0.0
10	0.0	0.0	0.0	1.8786108966117279	0.0
11	0.0	0.0	0.0	2.100775201309151	0.0
12	0.0	0.0	0.0	2.2906273404520214	0.0
13	0.0	0.0	0.0	2.386594956589308	0.0
14	0.0	0.0	0.0	2.4614409772516543	0.0
15	0.0	0.0	0.0	2.6910656620021527	0.0
16	0.0	0.0	0.0	2.9614317728866535	0.0
17	0.0	0.0	0.0	3.2865396335112522	0.0
18	0.0	0.0	0.0	3.4806064029438466	0.0
19	0.0	0.0	0.0	3.699524957877919	0.0
20	9.688805263734123E-5	0.0	0.0	3.959475603103906	0.0
21	9.688805263734123E-5	0.0	0.0	4.231198146725329	0.0
22	1.4533207895601186E-4	0.0	0.0	4.511931279242026	0.0
23	1.4533207895601186E-4	0.0	0.0	4.746933250913896	0.0
24	2.422201315933531E-4	0.0	0.0	4.962848276216212	0.0
25	2.422201315933531E-4	0.0	0.0	5.172853130307649	0.0
26	2.422201315933531E-4	0.0	0.0	5.406934665479465	0.0
27	2.422201315933531E-4	0.0	0.0	5.642663297546116	0.0
28	2.422201315933531E-4	0.0	0.0	5.899949521324576	0.0
29	2.422201315933531E-4	0.0	0.0	6.174675594577757	0.0
30	2.422201315933531E-4	0.0	0.0	6.4487234514624765	0.0
31	2.422201315933531E-4	0.0	0.0	6.711290074109671	0.0
32	2.422201315933531E-4	0.0	0.0	7.006750190627243	0.0
33	2.422201315933531E-4	0.0	0.0	7.295476587486521	0.0
34	2.422201315933531E-4	0.0	0.0	7.618259134847823	0.0
35	2.422201315933531E-4	0.0	0.0	7.9005424762067165	0.0
36	2.422201315933531E-4	0.0	0.0	8.212861113883186	0.0
37	2.422201315933531E-4	0.0	0.0	8.567616718614811	0.0
38	2.422201315933531E-4	0.0	0.0	8.905610690240175	0.0
39	2.422201315933531E-4	0.0	0.0	9.32780037960739	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCAA	20	7.034594E-4	45.0	29
TGCGTAC	20	7.034594E-4	45.0	24
CGTAATC	25	3.89189E-5	44.999996	33
CGTTTTT	23445	0.0	44.14587	1
TCACGAC	655	0.0	43.625957	24
CGGTCTA	610	0.0	43.524586	30
ACGGTCT	620	0.0	43.185482	29
CACGACG	645	0.0	42.55814	25
CCGCACT	2895	0.0	41.891193	33
CGACGGT	640	0.0	41.835938	27
CTAGCGG	540	0.0	41.666668	1
CCCGCAC	2945	0.0	41.56197	32
ACGGGAT	750	0.0	41.100002	4
ACCCGCA	2940	0.0	41.09694	31
CACTTAC	2965	0.0	41.053963	36
CGTTTTA	1245	0.0	41.024097	1
CGCACTT	2960	0.0	40.89527	34
ATCAACG	3160	0.0	40.656647	14
GACGGTC	660	0.0	40.56818	28
CAACGCA	3165	0.0	40.379147	16
>>END_MODULE