FastQCFastQC Report
Thu 26 May 2016
SRR1043444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043444_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3636948
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1354423.724056544113361No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC306330.8422721468659987No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146600.40308522420447035No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC119460.3284622161218692No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC98000.2694566983085818No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61160.1681629762097231No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC60540.16645825015919943No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC53320.14660644034503656No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA50770.13959506707272143No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA207.035304E-445.019
CGTTTTT378400.043.691861
CGGTCTA11450.041.65938630
TCACGAC11500.041.4782624
CACGACG11350.041.0352425
CACTTAC101950.040.7184936
CGACGGT11550.040.71428727
ACTTACT101850.040.53755637
CGCACTT102950.040.30111334
GACGGTC11850.040.25316628
TAGTAGG7950.039.9056631
GCACTTA104200.039.86084435
CTTACTG103750.039.8602438
CGTTTTA19800.039.6590921
ACCCGCA104550.039.59827831
CATGCGG5350.039.532711
CCCGCAC105900.039.4334332
CCGCACT105350.039.34029433
GGGCGAT36150.039.3360986
ACGTAGG3500.039.2142871