##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043444_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3636948
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.543319288590325	31.0	31.0	33.0	30.0	34.0
2	30.550431570646598	31.0	31.0	34.0	26.0	34.0
3	31.547377911369644	31.0	31.0	34.0	28.0	34.0
4	35.164763697473816	37.0	35.0	37.0	32.0	37.0
5	35.494828081127366	37.0	35.0	37.0	33.0	37.0
6	35.782358999908716	37.0	35.0	37.0	35.0	37.0
7	33.664853058113565	37.0	35.0	37.0	32.0	37.0
8	34.7043856552252	37.0	35.0	37.0	31.0	37.0
9	36.73219221171158	39.0	37.0	39.0	32.0	39.0
10	36.772178760873125	39.0	35.0	39.0	32.0	39.0
11	36.70226904536441	39.0	35.0	39.0	33.0	39.0
12	36.72778934425238	39.0	35.0	39.0	33.0	39.0
13	36.768241393608044	39.0	35.0	39.0	33.0	39.0
14	38.022411098536466	40.0	37.0	41.0	33.0	41.0
15	38.115948042149626	40.0	37.0	41.0	33.0	41.0
16	37.97640549163749	40.0	36.0	41.0	33.0	41.0
17	37.92468245352972	40.0	36.0	41.0	33.0	41.0
18	37.71056556211417	39.0	36.0	41.0	33.0	41.0
19	37.49032842922142	39.0	36.0	41.0	33.0	41.0
20	37.20018323055485	39.0	35.0	41.0	33.0	41.0
21	37.10598089387035	39.0	35.0	41.0	32.0	41.0
22	37.04084138678914	38.0	35.0	41.0	32.0	41.0
23	36.95658722643271	38.0	35.0	40.0	32.0	41.0
24	36.826177883214164	38.0	35.0	40.0	32.0	41.0
25	36.73013306761604	38.0	35.0	40.0	32.0	41.0
26	36.45489102401244	38.0	35.0	40.0	31.0	41.0
27	36.31740624281678	38.0	35.0	40.0	31.0	41.0
28	36.14327012649067	38.0	35.0	40.0	30.0	41.0
29	36.059676135045095	38.0	35.0	40.0	30.0	41.0
30	35.93156652225987	38.0	35.0	40.0	30.0	41.0
31	35.660539826249924	38.0	35.0	40.0	29.0	41.0
32	35.52756871970674	38.0	35.0	40.0	27.0	41.0
33	35.26975090103021	38.0	34.0	40.0	26.0	41.0
34	35.140746307068454	38.0	34.0	40.0	25.0	41.0
35	34.955463756974254	38.0	34.0	40.0	24.0	41.0
36	34.80008100198298	37.0	34.0	40.0	24.0	41.0
37	34.65553865493815	37.0	34.0	40.0	23.0	41.0
38	34.5246753596697	37.0	34.0	40.0	23.0	41.0
39	34.38188833054528	37.0	34.0	40.0	23.0	41.0
40	34.25661818645744	36.0	34.0	40.0	22.0	41.0
41	34.14165173656593	36.0	33.0	40.0	22.0	41.0
42	34.02218838432664	36.0	33.0	40.0	21.0	41.0
43	33.850471604213205	36.0	33.0	40.0	21.0	41.0
44	33.63102524424325	35.0	33.0	40.0	20.0	41.0
45	33.40371734762224	35.0	33.0	40.0	20.0	41.0
46	33.3845853721307	35.0	33.0	40.0	20.0	41.0
47	33.34543001439668	35.0	33.0	40.0	20.0	41.0
48	33.235077598029996	35.0	33.0	39.0	20.0	41.0
49	33.17827557611492	35.0	33.0	39.0	20.0	41.0
50	33.09218388604951	35.0	33.0	39.0	20.0	41.0
51	32.66564438094798	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	4.0
11	6.0
12	16.0
13	31.0
14	77.0
15	162.0
16	424.0
17	921.0
18	1939.0
19	3567.0
20	6044.0
21	9917.0
22	15362.0
23	23560.0
24	36554.0
25	54304.0
26	71335.0
27	82759.0
28	87035.0
29	89829.0
30	96549.0
31	111491.0
32	134992.0
33	176413.0
34	293538.0
35	353873.0
36	382001.0
37	417866.0
38	517089.0
39	668891.0
40	396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.337662237678405	16.763863547127976	22.93051756582717	29.967956649366446
2	32.588258066928645	20.20694274430099	26.465129553680722	20.73966963508964
3	26.33837492314985	21.023314053431612	33.047461772893094	19.59084925052544
4	23.262471720794466	23.927259889335783	30.727137149060148	22.083131240809603
5	21.588623208250436	33.423326371452106	28.947348161150504	16.040702259146954
6	80.45152144050451	2.332367688512456	15.735858747499275	1.4802521234837562
7	75.62304987588494	7.806572983721516	14.87051780778829	1.6998593326052505
8	74.23512791494406	4.160961333513705	18.25266679644581	3.351243955096416
9	41.031738699590974	19.328568898977935	24.934258064728997	14.705434336702092
10	26.395648219331154	19.963496866053625	31.418321075803117	22.222533838812105
11	23.001373679249745	16.572026875281143	40.09237965458951	20.334219790879608
12	21.064639912366083	18.06228739041636	40.40376711462468	20.469305582592877
13	18.609916886356363	21.995062893393033	40.771685490141735	18.62333473010887
14	17.829207346379437	19.76354899767607	41.421955991672135	20.985287664272352
15	21.238659447426798	16.59982490813726	41.004105640223614	21.157410004212323
16	21.941611483034677	17.26947979459701	36.83800263297688	23.950906089391435
17	21.36901599912894	20.032070846215014	37.14394596788296	21.454967186773086
18	23.56462066545906	22.074635106138444	33.52151309284598	20.839231135556517
19	24.924194681914617	19.781393630043652	33.300916042791926	21.99349564524981
20	24.50829651674976	21.186142886838084	36.009890710562814	18.295669885849346
21	23.16615469893988	18.11736104008086	34.897062042129825	23.819422218849432
22	23.22735986326997	20.833567045775744	33.75374077385764	22.18533231709664
23	22.1461236179346	20.399439310102867	36.72131138526039	20.73312568670215
24	25.180151049726305	18.25698360273504	34.7053628481903	21.857502499348357
25	20.6837986135628	20.876955073319717	34.24464688524554	24.19459942787194
26	19.15974602881317	20.746186087895673	36.26642448558517	23.827643397705987
27	21.000107782679322	21.222739505761425	36.952824181154085	20.824328530405165
28	22.464165008683104	21.690851780118937	34.597112743982045	21.247870467215918
29	20.760593772580744	20.548107919057408	37.29585905544979	21.39543925291206
30	25.56495171225984	20.908437514091485	32.458781373833226	21.067829399815448
31	24.359215474073316	21.692831461984056	33.083453489024315	20.86449957491831
32	24.54470066660288	20.53133561436677	32.172854822230065	22.751108896800286
33	21.51933434297108	22.396250922476757	34.17684278136504	21.907571953187123
34	22.397020798757637	22.544039672824578	32.754688821506384	22.3042507069114
35	25.696023149079945	24.16974342223205	30.730161663020755	19.404071765667258
36	23.85552391730649	23.566600347324183	30.560898863552627	22.0169768718167
37	23.473802759896483	26.922188604291293	29.386287623578887	20.217721012233334
38	22.984711356884947	23.011189601830985	29.67364394541797	24.330455095866093
39	22.454624041916464	22.225035936724968	33.86864480877923	21.451695212579338
40	21.20610467897809	22.5630941107764	33.8539346726981	22.376866537547414
41	22.139799634198784	25.332641544503797	29.43594464369576	23.09161417760166
42	20.820891582722655	21.973973782413168	31.320244336735087	25.884890298129093
43	21.675289281012542	21.79486756478234	34.18839092557826	22.34145222862686
44	23.313256059751197	23.760334214291763	31.921627694429507	21.004782031527533
45	23.85626629800591	23.149519872156542	31.693744315288537	21.30046951454901
46	22.348628575387934	24.15830526034466	31.33165500304101	22.16141116122639
47	22.10897708738206	25.71815709215529	31.847032181928363	20.325833638534288
48	22.93068253931593	24.97635379994435	31.381312023157882	20.71165163758184
49	21.605890433407353	23.03073896024909	33.547468921744276	21.815901684599286
50	21.20145792571134	23.623131262806066	33.83721735917038	21.33819345231221
51	21.0637325581779	20.98366542496621	34.12792814194759	23.824673874908303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2048.0
1	16839.5
2	31631.0
3	113663.5
4	195696.0
5	119417.0
6	43138.0
7	39625.0
8	36112.0
9	36255.0
10	36398.0
11	36236.0
12	36074.0
13	34814.0
14	33554.0
15	31407.0
16	29260.0
17	27739.5
18	26219.0
19	25110.0
20	24001.0
21	23067.5
22	22134.0
23	21831.0
24	21528.0
25	21954.5
26	24787.5
27	27194.0
28	29686.5
29	32179.0
30	35434.5
31	38690.0
32	43290.5
33	47891.0
34	56925.0
35	65959.0
36	70570.0
37	75181.0
38	79031.0
39	82881.0
40	90928.0
41	98975.0
42	113915.5
43	128856.0
44	145080.5
45	161305.0
46	173636.0
47	185967.0
48	208647.5
49	231328.0
50	217802.0
51	204276.0
52	185693.5
53	167111.0
54	170484.5
55	173858.0
56	182200.0
57	190542.0
58	197135.5
59	203729.0
60	199822.0
61	195915.0
62	192035.0
63	188155.0
64	174743.0
65	161331.0
66	141259.0
67	121187.0
68	102539.0
69	83891.0
70	75092.5
71	66294.0
72	61885.0
73	57476.0
74	48961.0
75	31462.5
76	22479.0
77	17074.0
78	11669.0
79	8838.5
80	6008.0
81	4597.0
82	3186.0
83	2327.5
84	1469.0
85	1141.0
86	813.0
87	578.0
88	343.0
89	238.0
90	133.0
91	83.0
92	33.0
93	25.0
94	17.0
95	11.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3636948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.054071572690123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90943404708831	19.519485368035312
2	8.897628478661845	4.458436414631996
3	2.911622400062425	2.188439880114353
4	1.4608804153677561	1.4640400994306013
5	0.8964410946038628	1.1229749672452927
6	0.6465246403607293	0.9718844767863272
7	0.4923656569312764	0.8635035086083552
8	0.37926308892472493	0.7601667659839677
9	0.3148938554864377	0.7100435873841795
>10	4.58980082337253	28.374446410924254
>50	1.233797548437096	21.152726958112737
>100	0.2574723997464906	9.555645455925063
>500	0.006231002389252307	1.1032109608585285
>1k	0.002586453821953764	1.1258324703885938
>5k	5.87830414080401E-4	0.9537263763987305
>10k+	4.7026433126432074E-4	5.675436299171802
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	135442	3.724056544113361	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	30633	0.8422721468659987	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14660	0.40308522420447035	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	11946	0.3284622161218692	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9800	0.2694566983085818	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6116	0.1681629762097231	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6054	0.16645825015919943	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5332	0.14660644034503656	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5077	0.13959506707272143	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04869467476576514	0.0
2	0.0	0.0	0.0	0.14776125476635904	0.0
3	0.0	0.0	0.0	0.23470228334306678	0.0
4	0.0	0.0	0.0	0.29478012883329646	0.0
5	0.0	0.0	0.0	0.6290714082246983	0.0
6	0.0	0.0	0.0	0.7528840115393456	0.0
7	0.0	0.0	0.0	1.08371084766678	0.0
8	0.0	0.0	0.0	1.4174247198475205	0.0
9	0.0	0.0	0.0	1.729307100348974	0.0
10	0.0	0.0	0.0	1.9305472610551484	0.0
11	0.0	0.0	0.0	2.145370239002592	0.0
12	0.0	0.0	0.0	2.3242839875631987	0.0
13	0.0	0.0	0.0	2.416504167780238	0.0
14	0.0	0.0	0.0	2.4816412002591184	0.0
15	0.0	0.0	0.0	2.678674537001904	0.0
16	0.0	0.0	0.0	2.891682806572984	0.0
17	0.0	0.0	0.0	3.17700445538402	0.0
18	0.0	0.0	0.0	3.329027525276688	0.0
19	0.0	0.0	0.0	3.512533035941124	0.0
20	0.0	0.0	0.0	3.71894236596179	0.0
21	2.749558146005937E-5	0.0	0.0	3.9326380250693713	0.0
22	2.749558146005937E-5	0.0	0.0	4.146168710688192	0.0
23	2.749558146005937E-5	0.0	0.0	4.342789613709077	0.0
24	5.499116292011874E-5	0.0	0.0	4.502318977340342	0.0
25	5.499116292011874E-5	0.0	0.0	4.666495094238356	0.0
26	5.499116292011874E-5	0.0	0.0	4.843594134422598	0.0
27	5.499116292011874E-5	0.0	0.0	5.033643593474529	0.0
28	5.499116292011874E-5	0.0	0.0	5.218303918560287	0.0
29	5.499116292011874E-5	0.0	0.0	5.42325598276357	0.0
30	5.499116292011874E-5	0.0	0.0	5.621059195787237	0.0
31	5.499116292011874E-5	0.0	0.0	5.820402161372668	0.0
32	5.499116292011874E-5	0.0	0.0	6.050402700286064	0.0
33	5.499116292011874E-5	0.0	0.0	6.255574729141027	0.0
34	8.248674438017811E-5	0.0	0.0	6.505097130891066	0.0
35	8.248674438017811E-5	0.0	0.0	6.721487356981733	0.0
36	8.248674438017811E-5	0.0	0.0	6.960039021729208	0.0
37	8.248674438017811E-5	0.0	0.0	7.226801153054704	0.0
38	8.248674438017811E-5	0.0	0.0	7.511765359306759	0.0
39	8.248674438017811E-5	0.0	0.0	7.840007610776948	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCA	20	7.035304E-4	45.0	19
CGTTTTT	37840	0.0	43.69186	1
CGGTCTA	1145	0.0	41.659386	30
TCACGAC	1150	0.0	41.47826	24
CACGACG	1135	0.0	41.03524	25
CACTTAC	10195	0.0	40.71849	36
CGACGGT	1155	0.0	40.714287	27
ACTTACT	10185	0.0	40.537556	37
CGCACTT	10295	0.0	40.301113	34
GACGGTC	1185	0.0	40.253166	28
TAGTAGG	795	0.0	39.905663	1
GCACTTA	10420	0.0	39.860844	35
CTTACTG	10375	0.0	39.86024	38
CGTTTTA	1980	0.0	39.659092	1
ACCCGCA	10455	0.0	39.598278	31
CATGCGG	535	0.0	39.53271	1
CCCGCAC	10590	0.0	39.43343	32
CCGCACT	10535	0.0	39.340294	33
GGGCGAT	3615	0.0	39.336098	6
ACGTAGG	350	0.0	39.214287	1
>>END_MODULE