FastQCFastQC Report
Thu 26 May 2016
SRR1043443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043443_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences868561
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482085.5503297983676445No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49970.5753194076178876No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20010.2303810555620158No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT11600.13355423510841494No Hit
GAAAGGGGCCTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCT10350.11916261494587024No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8960.10315913332512051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC302.1648866E-645.00000440
CCAATCG453.8380676E-1045.00000423
ATTCGGT302.1648866E-645.00000443
AACGGGT453.8380676E-1045.0000043
CCCCGAT302.1648866E-645.00000439
GGACGTT302.1648866E-645.0000048
ACCACGG600.045.0000041
TGGGCGC453.8380676E-1045.0000045
AGTAGCG453.8380676E-1045.00000432
ATACCGT302.1648866E-645.00000410
CTTACGG453.8380676E-1045.0000041
CATATTG253.8900158E-545.014
CGTTGCA207.0323347E-445.039
TCGGGTA207.0323347E-445.04
ATTGTCG207.0323347E-445.041
GCGTTGC207.0323347E-445.038
GCCATAC207.0323347E-445.012
ATCGTTC207.0323347E-445.036
ACCTCGG207.0323347E-445.01
GCACCGC207.0323347E-445.026