##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043443_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	868561
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54279549738015	31.0	31.0	33.0	30.0	34.0
2	30.580741018765522	31.0	31.0	34.0	26.0	34.0
3	31.497491828438072	31.0	31.0	34.0	28.0	34.0
4	35.12012627783196	37.0	35.0	37.0	32.0	37.0
5	35.433141713708075	37.0	35.0	37.0	33.0	37.0
6	35.810121568893834	37.0	35.0	37.0	35.0	37.0
7	33.764957210834936	37.0	35.0	37.0	32.0	37.0
8	34.79935318302341	37.0	35.0	37.0	32.0	37.0
9	36.76567218652461	39.0	37.0	39.0	32.0	39.0
10	36.73025153098055	39.0	35.0	39.0	32.0	39.0
11	36.387588206239975	38.0	35.0	39.0	32.0	39.0
12	36.220924034120806	39.0	35.0	39.0	32.0	39.0
13	36.28404452882411	39.0	35.0	39.0	32.0	39.0
14	37.650169648418476	40.0	35.0	41.0	33.0	41.0
15	37.830341219557404	40.0	35.0	41.0	33.0	41.0
16	37.79207793119885	40.0	35.0	41.0	33.0	41.0
17	37.79550313679753	40.0	36.0	41.0	33.0	41.0
18	37.51319481302983	39.0	36.0	41.0	33.0	41.0
19	37.23943165764984	38.0	35.0	41.0	33.0	41.0
20	36.939076242198304	38.0	35.0	41.0	33.0	41.0
21	36.85046300720387	38.0	35.0	41.0	32.0	41.0
22	36.77026253769165	38.0	35.0	40.0	32.0	41.0
23	36.686453801172284	38.0	35.0	40.0	32.0	41.0
24	36.544464925319005	37.0	35.0	40.0	32.0	41.0
25	36.44872150603124	37.0	35.0	40.0	32.0	41.0
26	36.19233306584108	37.0	35.0	40.0	31.0	41.0
27	36.11655830736126	36.0	35.0	40.0	31.0	41.0
28	36.05244997184999	36.0	35.0	40.0	31.0	41.0
29	35.99468661383599	36.0	35.0	40.0	31.0	41.0
30	35.8296147305716	36.0	35.0	40.0	31.0	41.0
31	35.410457066343064	36.0	35.0	40.0	30.0	41.0
32	35.268693851093936	36.0	35.0	40.0	29.0	41.0
33	35.15617671067432	36.0	35.0	40.0	27.0	41.0
34	34.97577372228318	36.0	35.0	40.0	26.0	41.0
35	34.76255553726221	36.0	34.0	40.0	25.0	41.0
36	34.60208321580177	36.0	34.0	40.0	24.0	41.0
37	34.54209088365699	36.0	34.0	40.0	24.0	41.0
38	34.45979269158988	36.0	34.0	40.0	23.0	41.0
39	34.31789477077603	35.0	34.0	40.0	23.0	41.0
40	34.285041580268974	35.0	34.0	40.0	23.0	41.0
41	34.18924750247824	35.0	34.0	40.0	23.0	41.0
42	34.098812864036034	35.0	34.0	40.0	22.0	41.0
43	33.93903479433224	35.0	34.0	40.0	21.0	41.0
44	33.743512545463126	35.0	33.0	40.0	21.0	41.0
45	33.57927077084972	35.0	33.0	40.0	20.0	41.0
46	33.58724603107899	35.0	33.0	40.0	20.0	41.0
47	33.59176039449158	35.0	33.0	40.0	20.0	41.0
48	33.526507637344984	35.0	33.0	40.0	20.0	41.0
49	33.428073560751635	35.0	33.0	40.0	20.0	41.0
50	33.31855102865544	35.0	33.0	40.0	20.0	41.0
51	32.88461259485517	35.0	33.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	4.0
13	11.0
14	30.0
15	42.0
16	90.0
17	188.0
18	414.0
19	848.0
20	1419.0
21	2339.0
22	3741.0
23	5673.0
24	9388.0
25	13942.0
26	17194.0
27	18445.0
28	18602.0
29	19358.0
30	22038.0
31	26045.0
32	32843.0
33	43948.0
34	78870.0
35	116639.0
36	92267.0
37	77897.0
38	98592.0
39	167602.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.826785913712452	18.906559239938243	24.954378564084735	29.31227628226457
2	30.81004097582093	21.59099936561738	29.266223097744433	18.33273656081726
3	21.435685000823202	21.797202499306323	37.271878428803504	19.49523407106697
4	22.85446848292751	25.347327361002854	33.880176521856264	17.91802763421337
5	19.332090664904364	32.2802888916265	32.045532783535066	16.342087659934073
6	79.55607032781809	2.402940035299766	16.894956140098394	1.1460334967837609
7	76.28640935984922	7.211468164009206	15.177748022303557	1.3243744538380149
8	76.5060830500103	3.570042864001492	17.585408509016638	2.338465576971566
9	39.843833651292194	25.85068866780802	24.16779017248069	10.137687508419098
10	31.286691435604407	23.42518257209338	34.03952054029596	11.248605452006249
11	29.78432142359604	21.8217258200633	35.52036068854116	12.873592067799496
12	17.88820819723658	34.637060609444816	33.381190267580514	14.093540925738088
13	12.97398801005341	36.11041711520549	34.15557456528672	16.760020309454372
14	10.79210326045033	25.796000511190346	47.6656216431546	15.746274585204723
15	13.477234183897274	22.447933996575944	45.51263526683791	18.562196552688874
16	14.088935607286073	23.967343686856765	35.00836440963847	26.93535629621869
17	16.403914060152367	24.05829872628405	41.573476128907465	17.964311084656114
18	20.254881349726734	25.873600127106787	34.6956632867467	19.17585523641978
19	22.21386868625232	25.62157407482031	34.9854529503397	17.179104288587677
20	16.061163234361203	29.95805706219828	36.16314801148106	17.81763169195946
21	18.46122494562846	25.501260130261432	33.2792975968297	22.758217327280413
22	12.987458566525554	29.47231109847207	34.088106649964715	23.452123685037666
23	12.878197386251514	24.761530853906635	46.758489041069076	15.601782718772775
24	13.358071568951404	27.664493340133856	41.47434664922786	17.50308844168688
25	13.554143002045912	32.89843776084812	34.76681545683032	18.78060378027565
26	12.436086814858138	34.13922568478207	35.49318930967428	17.931498190685513
27	12.268338090243518	30.056495744110084	40.722873810820424	16.95229235482597
28	12.452550828324089	25.073886577914507	40.279151377968844	22.19441121579256
29	13.893554971959368	31.52593772918655	37.553148253260275	17.027359045593805
30	19.91662070942628	30.555136599501935	33.30739003938699	16.220852651684798
31	19.636271948659907	27.59875241923135	34.59331008415068	18.17166554795806
32	18.91266128688716	31.24317117623287	32.34982920025191	17.494338336628054
33	14.880935248071234	29.36546771038534	33.661308762424284	22.092288279119142
34	15.227255195662712	28.501970500632655	35.415244294873936	20.855530008830698
35	22.23321102375078	28.93245264293469	31.885958499172766	16.94837783414176
36	15.492521538498735	33.07424579275376	34.164554936268146	17.268677732479354
37	15.882822277306948	31.639689094951308	33.472720971814304	19.00476765592745
38	15.535351000102468	33.77379366561474	32.26796966476736	18.42288566951544
39	18.677674912873133	29.25943025302771	32.044266320960766	20.0186285131384
40	14.740818434168702	28.0332642151789	35.16978082138157	22.056136529270827
41	16.259076794836517	29.41083009713768	31.777157850743933	22.552935257281874
42	16.56843906185058	27.654016240655523	33.522803809979955	22.254740887513947
43	15.697227943690773	29.22730815682491	33.715996919041956	21.359466980442363
44	13.607334430166679	37.814845474295986	29.07855637082485	19.499263724712485
45	17.846069533400648	32.40831674459249	31.451331570263918	18.29428215174294
46	15.988859734664576	32.76200520170719	31.240983649968168	20.008151413660066
47	15.902049481844108	29.699007899272473	33.62423594888557	20.774706669997848
48	17.520243252920636	27.56398226491864	35.51702183266345	19.398752649497272
49	17.54787516363272	31.75067726964485	31.784065828421955	18.917381738300477
50	14.868616021212096	31.499111749203568	31.347942171016197	22.284330058568138
51	14.921922582294162	28.41631157742519	35.61949016822077	21.04227567205988
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	882.0
1	4841.0
2	8800.0
3	33755.5
4	58711.0
5	34211.5
6	9712.0
7	9716.0
8	9720.0
9	11563.5
10	13407.0
11	14896.0
12	16385.0
13	16504.5
14	16624.0
15	14993.0
16	13362.0
17	11477.5
18	9593.0
19	8448.5
20	7304.0
21	7063.0
22	6822.0
23	6214.5
24	5607.0
25	6402.5
26	7689.0
27	8180.0
28	9481.0
29	10782.0
30	12314.5
31	13847.0
32	16572.5
33	19298.0
34	23324.0
35	27350.0
36	29334.5
37	31319.0
38	33028.5
39	34738.0
40	41176.0
41	47614.0
42	54812.5
43	62011.0
44	65839.5
45	69668.0
46	67133.0
47	64598.0
48	60757.0
49	56916.0
50	55959.0
51	55002.0
52	52051.5
53	49101.0
54	42175.5
55	35250.0
56	31273.5
57	27297.0
58	24625.5
59	21954.0
60	18229.0
61	14504.0
62	12117.5
63	9731.0
64	7974.5
65	6218.0
66	5342.5
67	4467.0
68	4040.0
69	3613.0
70	3277.5
71	2942.0
72	2974.0
73	3006.0
74	2641.5
75	1829.0
76	1381.0
77	1013.0
78	645.0
79	499.5
80	354.0
81	288.0
82	222.0
83	154.5
84	87.0
85	65.5
86	44.0
87	26.5
88	9.0
89	5.0
90	1.0
91	2.0
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	868561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.22122055835218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41430309250335	24.481902517805246
2	9.516387069492122	5.942264392305285
3	3.4216760472611782	3.2048670765231573
4	1.7413619065030952	2.1746977661938316
5	1.1502611454815892	1.7956278461391761
6	0.7646717761115888	1.432439170603609
7	0.5333901428116709	1.16571639046619
8	0.3889475402230898	0.9714733551146915
9	0.3182844757850579	0.8943506836906318
>10	2.6459805786351915	18.35820301684242
>50	0.7960542882168027	17.864437224354617
>100	0.3021631879956854	13.895732863443122
>500	0.004601469867954601	0.9462636238156134
>1k	0.0015338232893182002	1.100582172790604
>5k	0.0	0.0
>10k+	3.8345582232955004E-4	5.771441899911828
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48208	5.5503297983676445	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4997	0.5753194076178876	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2001	0.2303810555620158	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1160	0.13355423510841494	No Hit
GAAAGGGGCCTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCT	1035	0.11916261494587024	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	896	0.10315913332512051	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06631658570900605	0.0
2	0.0	0.0	0.0	0.2369436343561362	0.0
3	0.0	0.0	0.0	0.390070472885612	0.0
4	0.0	0.0	0.0	0.4913874788299267	0.0
5	0.0	0.0	0.0	1.1334840040020218	0.0
6	0.0	0.0	0.0	1.307680174449463	0.0
7	0.0	0.0	0.0	1.8082782901834182	0.0
8	0.0	0.0	0.0	2.3677093491418564	0.0
9	0.0	0.0	0.0	2.8061356657736187	0.0
10	0.0	0.0	0.0	3.1220605115818003	0.0
11	0.0	0.0	0.0	3.476670032386902	0.0
12	0.0	0.0	0.0	3.777051928419535	0.0
13	0.0	0.0	0.0	3.9017409255078226	0.0
14	0.0	0.0	0.0	4.004784925871643	0.0
15	0.0	0.0	0.0	4.382421038936816	0.0
16	0.0	0.0	0.0	4.835123842769823	0.0
17	0.0	0.0	0.0	5.391676577695752	0.0
18	0.0	0.0	0.0	5.662469302674193	0.0
19	0.0	0.0	0.0	5.971025638959152	0.0
20	0.0	0.0	0.0	6.314006730672918	0.0
21	0.0	0.0	0.0	6.664931996716408	0.0
22	2.3026592260071545E-4	0.0	0.0	7.062140713202642	0.0
23	2.3026592260071545E-4	0.0	0.0	7.358147556705862	0.0
24	4.605318452014309E-4	0.0	0.0	7.617426985554268	0.0
25	4.605318452014309E-4	0.0	0.0	7.873597824447563	0.0
26	4.605318452014309E-4	0.0	0.0	8.144160283503403	0.0
27	4.605318452014309E-4	0.0	0.0	8.395840936905985	0.0
28	8.05930729102504E-4	0.0	0.0	8.674692969175453	0.0
29	8.05930729102504E-4	0.0	0.0	9.031374883283961	0.0
30	8.05930729102504E-4	0.0	0.0	9.351214249776355	0.0
31	8.05930729102504E-4	0.0	0.0	9.61981944849009	0.0
32	8.05930729102504E-4	0.0	0.0	9.907421585818382	0.0
33	8.05930729102504E-4	0.0	0.0	10.21229366734173	0.0
34	8.05930729102504E-4	0.0	0.0	10.57335063397965	0.0
35	8.05930729102504E-4	0.0	0.0	10.877762183657797	0.0
36	8.05930729102504E-4	0.0	0.0	11.168818309825102	0.0
37	8.05930729102504E-4	0.0	0.0	11.507539481970754	0.0
38	8.05930729102504E-4	0.0	0.0	11.844073127851699	0.0
39	8.05930729102504E-4	0.0	0.0	12.20743275371563	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	30	2.1648866E-6	45.000004	40
CCAATCG	45	3.8380676E-10	45.000004	23
ATTCGGT	30	2.1648866E-6	45.000004	43
AACGGGT	45	3.8380676E-10	45.000004	3
CCCCGAT	30	2.1648866E-6	45.000004	39
GGACGTT	30	2.1648866E-6	45.000004	8
ACCACGG	60	0.0	45.000004	1
TGGGCGC	45	3.8380676E-10	45.000004	5
AGTAGCG	45	3.8380676E-10	45.000004	32
ATACCGT	30	2.1648866E-6	45.000004	10
CTTACGG	45	3.8380676E-10	45.000004	1
CATATTG	25	3.8900158E-5	45.0	14
CGTTGCA	20	7.0323347E-4	45.0	39
TCGGGTA	20	7.0323347E-4	45.0	4
ATTGTCG	20	7.0323347E-4	45.0	41
GCGTTGC	20	7.0323347E-4	45.0	38
GCCATAC	20	7.0323347E-4	45.0	12
ATCGTTC	20	7.0323347E-4	45.0	36
ACCTCGG	20	7.0323347E-4	45.0	1
GCACCGC	20	7.0323347E-4	45.0	26
>>END_MODULE