FastQCFastQC Report
Thu 26 May 2016
SRR1043442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043442_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1052080
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT576055.4753440802980755No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62620.5952018857881529No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25000.23762451524598888No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.11139837274731959No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC11190.10636073302410463Illumina PCR Primer Index 7 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT302.165325E-645.00000441
CGTCTTA1200.045.00000437
CCGCTAA302.165325E-645.00000415
GACTTCG1200.045.00000432
ACGTCGA302.165325E-645.00000430
ACCAACG302.165325E-645.00000438
AACGTGC253.89058E-545.044
TAATACG253.89058E-545.015
CAGCGAC207.033016E-445.030
GTCGATT207.033016E-445.016
GTCGAAT253.89058E-545.016
TTAGCGA207.033016E-445.01
ACTTCGA207.033016E-445.031
CGATTTA253.89058E-545.08
CGCGATG253.89058E-545.013
TATTGCG253.89058E-545.035
TGCATCG253.89058E-545.035
GCCGATC207.033016E-445.08
CTACGGT253.89058E-545.022
CGAGGTT351.2117926E-745.05