##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043442_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1052080
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.550418219146835	31.0	31.0	33.0	30.0	34.0
2	30.543757128735457	31.0	31.0	34.0	26.0	34.0
3	31.476918105087066	31.0	31.0	34.0	28.0	34.0
4	35.09783001292677	37.0	35.0	37.0	32.0	37.0
5	35.41687134058247	37.0	35.0	37.0	33.0	37.0
6	35.84321249334651	37.0	35.0	37.0	35.0	37.0
7	33.66121967911185	37.0	35.0	37.0	32.0	37.0
8	34.74150825032317	37.0	35.0	37.0	31.0	37.0
9	36.77482795985096	39.0	37.0	39.0	32.0	39.0
10	36.67999581780853	39.0	35.0	39.0	32.0	39.0
11	36.46557961371759	38.0	35.0	39.0	32.0	39.0
12	36.36927705117481	39.0	35.0	39.0	32.0	39.0
13	36.42930575621626	39.0	35.0	39.0	32.0	39.0
14	37.78106797962132	40.0	36.0	41.0	33.0	41.0
15	37.92746654246825	40.0	36.0	41.0	33.0	41.0
16	37.883119154436926	40.0	36.0	41.0	33.0	41.0
17	37.8716551973234	40.0	36.0	41.0	33.0	41.0
18	37.602652840088204	39.0	36.0	41.0	33.0	41.0
19	37.317402668998554	38.0	35.0	41.0	33.0	41.0
20	37.02313702380047	38.0	35.0	41.0	33.0	41.0
21	36.914919968063266	38.0	35.0	41.0	32.0	41.0
22	36.82129685955441	38.0	35.0	40.0	32.0	41.0
23	36.74433313056041	38.0	35.0	40.0	32.0	41.0
24	36.60416508250323	38.0	35.0	40.0	32.0	41.0
25	36.51055813246141	37.0	35.0	40.0	32.0	41.0
26	36.22485362329861	37.0	35.0	40.0	31.0	41.0
27	36.16256653486427	37.0	35.0	40.0	31.0	41.0
28	36.06930366512052	37.0	35.0	40.0	31.0	41.0
29	36.026337350771804	37.0	35.0	40.0	31.0	41.0
30	35.82386415481712	36.0	35.0	40.0	31.0	41.0
31	35.405251501786935	36.0	35.0	40.0	29.0	41.0
32	35.23456581248574	37.0	35.0	40.0	27.0	41.0
33	35.125989468481485	37.0	35.0	40.0	27.0	41.0
34	34.95625237624515	37.0	35.0	40.0	25.0	41.0
35	34.72979146072542	37.0	34.0	40.0	24.0	41.0
36	34.56766975895369	36.0	34.0	40.0	23.0	41.0
37	34.497745418599344	36.0	34.0	40.0	23.0	41.0
38	34.406337921070644	36.0	34.0	40.0	23.0	41.0
39	34.2560271082047	36.0	34.0	40.0	22.0	41.0
40	34.19549749068512	36.0	34.0	40.0	22.0	41.0
41	34.10746331077485	36.0	34.0	40.0	21.0	41.0
42	34.008478442703975	35.0	33.0	40.0	21.0	41.0
43	33.83983347273971	35.0	33.0	40.0	20.0	41.0
44	33.629466390388565	35.0	33.0	40.0	18.0	41.0
45	33.44157288419132	35.0	33.0	40.0	18.0	41.0
46	33.45047810052468	35.0	33.0	40.0	20.0	41.0
47	33.44340734544902	35.0	33.0	40.0	20.0	41.0
48	33.39649361265303	35.0	33.0	40.0	19.0	41.0
49	33.3115304919778	35.0	33.0	40.0	18.0	41.0
50	33.177639533115354	35.0	33.0	40.0	18.0	41.0
51	32.75338947608547	35.0	32.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	3.0
13	9.0
14	25.0
15	47.0
16	110.0
17	251.0
18	507.0
19	1059.0
20	1745.0
21	2821.0
22	4484.0
23	7086.0
24	11703.0
25	17244.0
26	21811.0
27	23407.0
28	23927.0
29	24435.0
30	27098.0
31	31977.0
32	39829.0
33	52040.0
34	90320.0
35	129802.0
36	113490.0
37	98041.0
38	126729.0
39	201963.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.133849136947763	18.63318378830507	25.072523002053078	30.16044407269409
2	32.122081970952785	20.642441639419058	28.881739031252373	18.35373735837579
3	21.14639571135275	21.640559653258308	38.748954452132914	18.464090183256026
4	22.78020682837807	24.12126454262033	34.85942133678047	18.239107292221124
5	19.278096722682687	31.811934453653713	32.90709831951943	16.00287050414417
6	78.43253364763136	2.0601094973766254	17.885331913922894	1.6220249410691203
7	74.80153600486655	7.5122614249866935	16.087274731959546	1.5989278381872103
8	74.65154741084328	3.780606037563684	19.089803056801763	2.4780434947912706
9	38.75076039844878	27.332617291460725	24.19796973614174	9.718652573948749
10	27.950060831875902	23.136073302410463	37.93475781309406	10.979108052619573
11	26.874762375484757	20.888240437989506	37.85824271918485	14.378754467340887
12	17.856912021899475	30.807162953387575	36.87476237548476	14.461162649228196
13	13.92289559729298	33.17247737814615	36.57050794616379	16.33411907839708
14	13.21220819709528	24.939453273515323	46.36729146072542	15.481047068663981
15	14.711904037715762	23.100524674929662	45.75631130712494	16.43125998022964
16	15.26480875979013	23.01364915215573	37.59134286366056	24.130199224393582
17	16.637042810432668	23.13037031404456	41.80204927381948	18.430537601703293
18	19.931564139609158	25.044008060223554	37.6482776975135	17.37615010265379
19	21.42432134438446	25.26404836134134	36.66745874838415	16.644171545890046
20	17.491160368032848	28.50961904037716	37.397916508250326	16.60130408333967
21	18.24880237244316	24.46382404379895	36.273857501330696	21.01351608242719
22	14.371055433046918	28.002338225230023	36.59702684206525	21.02957949965782
23	13.438901984639951	24.060147517299065	46.313873469698116	16.18707702836286
24	15.068245760778648	25.480096570602996	42.02104402707019	17.43061364154817
25	14.189700403011177	31.00809824347958	36.59113375408714	18.211067599422098
26	13.999980990038779	31.49646414721314	37.0555471066839	17.448007756064175
27	13.602387651129192	28.88877271690366	40.52942741996806	16.979412211999087
28	13.429111854611817	25.272412744278	40.768002433275036	20.530472967835145
29	14.721694167743898	30.453007375864953	38.06991863736598	16.75537981902517
30	19.603452208957496	28.947038248041974	35.29569994677211	16.153809596228424
31	20.505664968443465	26.974659721694167	35.97074366968292	16.548931640179454
32	19.05919701923808	28.844859706486197	35.507946163789825	16.587997110485894
33	15.50176792639343	28.62006691506349	34.911223481104095	20.966941677438978
34	15.825317466352368	27.436316629914074	37.16371378602388	19.57465211770968
35	21.307885331913923	27.530035738727094	34.55830355106075	16.603775378298227
36	16.081001444757053	30.264143411147444	35.681221960307205	17.973633183788305
37	16.472511596076345	29.97433655235343	34.97167515778268	18.581476693787543
38	16.302277393354117	30.69728537753783	33.960440270701845	19.039996958406206
39	19.445954680252452	27.147840468405445	33.71768306592654	19.68852178541556
40	14.864839175728081	27.258003193673485	35.45462322256863	22.422534408029808
41	16.68722910805262	28.313531290396167	32.94492814234659	22.054311459204623
42	17.11267204014904	27.055832256102196	34.37352672800548	21.457968975743288
43	17.58535472587636	28.393848376549315	33.910063873469696	20.11073302410463
44	14.65763059843358	33.94247585734925	32.24783286442096	19.15206067979621
45	17.20087826020835	31.038229032012776	33.624629305756216	18.13626340202266
46	16.637137860238767	29.186848908828228	33.54887461029579	20.627138620637215
47	15.781499505741008	28.375693863584516	36.233841532963275	19.608965097711202
48	17.84427039768839	26.013896281651583	35.81220059311079	20.329632727549235
49	17.61073302410463	29.331989962740472	33.81026157706638	19.24701543608851
50	15.62504752490305	29.86740552049274	33.47331001444757	21.034236940156642
51	16.045833016500648	27.01952323017261	35.97036347045852	20.964280282868224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1126.0
1	6211.5
2	11297.0
3	41665.0
4	72033.0
5	42431.0
6	12829.0
7	12852.0
8	12875.0
9	14505.0
10	16135.0
11	17805.0
12	19475.0
13	19046.0
14	18617.0
15	17136.0
16	15655.0
17	13669.5
18	11684.0
19	10537.0
20	9390.0
21	8878.0
22	8366.0
23	7813.0
24	7260.0
25	8102.5
26	9950.0
27	10955.0
28	12095.0
29	13235.0
30	15200.0
31	17165.0
32	19564.5
33	21964.0
34	26512.0
35	31060.0
36	33066.0
37	35072.0
38	38591.5
39	42111.0
40	49660.0
41	57209.0
42	67864.0
43	78519.0
44	83922.5
45	89326.0
46	88831.5
47	88337.0
48	82372.0
49	76407.0
50	67762.0
51	59117.0
52	53800.0
53	48483.0
54	42698.0
55	36913.0
56	34139.0
57	31365.0
58	28260.0
59	25155.0
60	21511.0
61	17867.0
62	14979.0
63	12091.0
64	10646.0
65	9201.0
66	7905.5
67	6610.0
68	5316.5
69	4023.0
70	3798.0
71	3573.0
72	3807.5
73	4042.0
74	3617.5
75	2432.5
76	1672.0
77	1313.5
78	955.0
79	689.5
80	424.0
81	319.0
82	214.0
83	157.0
84	100.0
85	59.0
86	18.0
87	14.0
88	10.0
89	5.5
90	1.0
91	2.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1052080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.00433947781868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39083086795918	25.08846763046434
2	9.46051219027623	6.055548875432849
3	3.4932577517900043	3.3539822091542693
4	1.8454759240845524	2.3625295189017264
5	1.1101163619288184	1.776427045352547
6	0.7591066648842666	1.4576824441698488
7	0.5272701443827337	1.1812452888140437
8	0.4153112831633105	1.063341063626166
9	0.2907239803566033	0.837398606550788
>10	2.669500399838496	19.044773194696027
>50	0.7548042020913319	17.211348249816886
>100	0.27689569468770986	12.67335738614596
>500	0.0043361741897385	0.9844202407628801
>1k	0.001238906911353857	0.5785984236565231
>5k	3.0972672783846427E-4	0.6207269708646819
>10k+	3.0972672783846427E-4	5.710152851590546
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57605	5.4753440802980755	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6262	0.5952018857881529	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2500	0.23762451524598888	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1172	0.11139837274731959	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1119	0.10636073302410463	Illumina PCR Primer Index 7 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10550528476921907	0.0
2	0.0	0.0	0.0	0.3461713938103566	0.0
3	0.0	0.0	0.0	0.5508136263402023	0.0
4	0.0	0.0	0.0	0.6877803969279903	0.0
5	0.0	0.0	0.0	1.509771120066915	0.0
6	0.0	0.0	0.0	1.70557372062961	0.0
7	0.0	0.0	0.0	2.3148429777203257	0.0
8	0.0	0.0	0.0	3.034845258915672	0.0
9	0.0	0.0	0.0	3.5514409550604515	0.0
10	0.0	0.0	0.0	3.9288837350771804	0.0
11	0.0	0.0	0.0	4.396528781081287	0.0
12	0.0	0.0	0.0	4.7745418599346054	0.0
13	0.0	0.0	0.0	4.93127899019086	0.0
14	0.0	0.0	0.0	5.061972473576154	0.0
15	0.0	0.0	0.0	5.515550148277698	0.0
16	0.0	0.0	0.0	6.070926165310623	0.0
17	0.0	0.0	0.0	6.748156033761691	0.0
18	0.0	0.0	0.0	7.083206600258536	0.0
19	0.0	0.0	0.0	7.4718652573948745	0.0
20	0.0	0.0	0.0	7.883621017413124	0.0
21	0.0	0.0	0.0	8.30088966618508	0.0
22	9.504980609839556E-5	0.0	0.0	8.750190099612198	0.0
23	4.752490304919778E-4	0.0	0.0	9.110523914531214	0.0
24	0.0018059463158695156	0.0	0.0	9.42475857349251	0.0
25	0.0018059463158695156	0.0	0.0	9.724450612120751	0.0
26	0.0018059463158695156	0.0	0.0	10.034788229032014	0.0
27	0.0018059463158695156	0.0	0.0	10.33828225990419	0.0
28	0.0022811953463614935	0.0	0.0	10.660691202189948	0.0
29	0.0022811953463614935	0.0	0.0	11.07520340658505	0.0
30	0.0022811953463614935	0.0	0.0	11.442285757737054	0.0
31	0.0022811953463614935	0.0	0.0	11.761082807391073	0.0
32	0.0022811953463614935	0.0	0.0	12.0853927457988	0.0
33	0.0022811953463614935	0.0	0.0	12.447817656451981	0.0
34	0.0022811953463614935	0.0	0.0	12.84826248954452	0.0
35	0.0022811953463614935	0.0	0.0	13.222568625960003	0.0
36	0.0022811953463614935	0.0	0.0	13.574728157554558	0.0
37	0.0022811953463614935	0.0	0.0	13.943521405216334	0.0
38	0.0022811953463614935	0.0	0.0	14.31754239221352	0.0
39	0.0022811953463614935	0.0	0.0	14.709527792563303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	30	2.165325E-6	45.000004	41
CGTCTTA	120	0.0	45.000004	37
CCGCTAA	30	2.165325E-6	45.000004	15
GACTTCG	120	0.0	45.000004	32
ACGTCGA	30	2.165325E-6	45.000004	30
ACCAACG	30	2.165325E-6	45.000004	38
AACGTGC	25	3.89058E-5	45.0	44
TAATACG	25	3.89058E-5	45.0	15
CAGCGAC	20	7.033016E-4	45.0	30
GTCGATT	20	7.033016E-4	45.0	16
GTCGAAT	25	3.89058E-5	45.0	16
TTAGCGA	20	7.033016E-4	45.0	1
ACTTCGA	20	7.033016E-4	45.0	31
CGATTTA	25	3.89058E-5	45.0	8
CGCGATG	25	3.89058E-5	45.0	13
TATTGCG	25	3.89058E-5	45.0	35
TGCATCG	25	3.89058E-5	45.0	35
GCCGATC	20	7.033016E-4	45.0	8
CTACGGT	25	3.89058E-5	45.0	22
CGAGGTT	35	1.2117926E-7	45.0	5
>>END_MODULE