FastQCFastQC Report
Thu 26 May 2016
SRR1043441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043441_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3006450
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1362234.5310249629962245No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC249610.8302482994894311No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139070.46257213657303464No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC102610.3412995393237872No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC85580.2846546591494953No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60620.2016331553825941No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA46230.1537693957990321No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC45280.15060952285918608No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC42410.14106338039880922No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG41850.13920071845532106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT362050.044.0864561
CGTTTTA17700.043.3474581
CACTTAC80300.041.4694936
CGCACTT80700.041.18029834
ATTACGG2350.041.170211
ACTTACT80450.041.08452637
GCACTTA81300.040.93173235
TCACGAC4900.040.86734824
ACCCGCA81650.040.5909431
CGGTCTA5100.040.58823430
CCGCACT81900.040.5769233
CTTACTG81800.040.29645538
CCCGCAC82850.040.1931232
GCGCGAC23950.039.5511478
TTATGAC85800.039.51923426
TATGACC85650.039.5096327
GCATAGG4450.039.43821
CATGCGG5150.039.3203931
CTTATGA86400.039.27083625
AACGCAA88150.039.205917